CA2542478C - Procede de dissociation de la molecule d'hemoglobine extracellulaire d'arenicola marina, caracterisation des chaines proteiques constituant ladite molecule et des sequences nucleotidiques codant pour lesdites chaines proteiques - Google Patents
Procede de dissociation de la molecule d'hemoglobine extracellulaire d'arenicola marina, caracterisation des chaines proteiques constituant ladite molecule et des sequences nucleotidiques codant pour lesdites chaines proteiques Download PDFInfo
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- CA2542478C CA2542478C CA2542478A CA2542478A CA2542478C CA 2542478 C CA2542478 C CA 2542478C CA 2542478 A CA2542478 A CA 2542478A CA 2542478 A CA2542478 A CA 2542478A CA 2542478 C CA2542478 C CA 2542478C
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- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 239000002473 artificial blood Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 230000036770 blood supply Effects 0.000 description 1
- UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 1
- 210000004899 c-terminal region Anatomy 0.000 description 1
- 238000010804 cDNA synthesis Methods 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 238000003981 capillary liquid chromatography Methods 0.000 description 1
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 1
- 150000007942 carboxylates Chemical group 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000012636 effector Substances 0.000 description 1
- 238000001437 electrospray ionisation time-of-flight quadrupole detection Methods 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 239000012149 elution buffer Substances 0.000 description 1
- 238000005562 fading Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000000834 fixative Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000011544 gradient gel Substances 0.000 description 1
- ZRALSGWEFCBTJO-UHFFFAOYSA-O guanidinium Chemical class NC(N)=[NH2+] ZRALSGWEFCBTJO-UHFFFAOYSA-O 0.000 description 1
- 238000003505 heat denaturation Methods 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 125000000487 histidyl group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C([H])=N1 0.000 description 1
- 230000009851 immunogenic response Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000001155 isoelectric focusing Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 238000004895 liquid chromatography mass spectrometry Methods 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000010413 mother solution Substances 0.000 description 1
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 description 1
- YFCUZWYIPBUQBD-ZOWNYOTGSA-N n-[(3s)-7-amino-1-chloro-2-oxoheptan-3-yl]-4-methylbenzenesulfonamide;hydron;chloride Chemical compound Cl.CC1=CC=C(S(=O)(=O)N[C@@H](CCCCN)C(=O)CCl)C=C1 YFCUZWYIPBUQBD-ZOWNYOTGSA-N 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000006213 oxygenation reaction Methods 0.000 description 1
- 239000006174 pH buffer Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
- WTWWXOGTJWMJHI-UHFFFAOYSA-N perflubron Chemical compound FC(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)Br WTWWXOGTJWMJHI-UHFFFAOYSA-N 0.000 description 1
- 229960001217 perflubron Drugs 0.000 description 1
- 229950011087 perflunafene Drugs 0.000 description 1
- UWEYRJFJVCLAGH-IJWZVTFUSA-N perfluorodecalin Chemical compound FC1(F)C(F)(F)C(F)(F)C(F)(F)[C@@]2(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)[C@@]21F UWEYRJFJVCLAGH-IJWZVTFUSA-N 0.000 description 1
- JRKICGRDRMAZLK-UHFFFAOYSA-L peroxydisulfate Chemical compound [O-]S(=O)(=O)OOS([O-])(=O)=O JRKICGRDRMAZLK-UHFFFAOYSA-L 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 150000004032 porphyrins Chemical class 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- NNFCIKHAZHQZJG-UHFFFAOYSA-N potassium cyanide Chemical compound [K+].N#[C-] NNFCIKHAZHQZJG-UHFFFAOYSA-N 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 239000012429 reaction media Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000008707 rearrangement Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000033764 rhythmic process Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 125000000467 secondary amino group Chemical group [H]N([*:1])[*:2] 0.000 description 1
- 230000000276 sedentary effect Effects 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000001338 self-assembly Methods 0.000 description 1
- 238000002864 sequence alignment Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 235000015424 sodium Nutrition 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- WROMPOXWARCANT-UHFFFAOYSA-N tfa trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F.OC(=O)C(F)(F)F WROMPOXWARCANT-UHFFFAOYSA-N 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- BSVBQGMMJUBVOD-UHFFFAOYSA-N trisodium borate Chemical compound [Na+].[Na+].[Na+].[O-]B([O-])[O-] BSVBQGMMJUBVOD-UHFFFAOYSA-N 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 239000002753 trypsin inhibitor Substances 0.000 description 1
- 210000002268 wool Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/795—Porphyrin- or corrin-ring-containing peptides
- C07K14/805—Haemoglobins; Myoglobins
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR0311992A FR2860796B1 (fr) | 2003-10-14 | 2003-10-14 | Procede de dissociation de la molecule d'hemoglobine extracellulaire d'arenicola marina, chaines proteiques constituant ladite molecule et sequences nucleotidiques codant pour lesdites chaines proteiques |
| FR0311992 | 2003-10-14 | ||
| PCT/FR2004/002602 WO2005037392A2 (fr) | 2003-10-14 | 2004-10-13 | Procede de dissociation de la molecule d'hemoglobine extracellulaire d'arenicola marina, caracterisation des chaines proteiques constituant ladite molecule et des sequences nucleotidiques codant pour lesdites chaines proteiques |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2542478A1 CA2542478A1 (fr) | 2005-04-28 |
| CA2542478C true CA2542478C (fr) | 2013-08-06 |
Family
ID=34355459
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2542478A Expired - Lifetime CA2542478C (fr) | 2003-10-14 | 2004-10-13 | Procede de dissociation de la molecule d'hemoglobine extracellulaire d'arenicola marina, caracterisation des chaines proteiques constituant ladite molecule et des sequences nucleotidiques codant pour lesdites chaines proteiques |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US7776526B2 (enExample) |
| EP (1) | EP1673155B1 (enExample) |
| JP (1) | JP2007508024A (enExample) |
| CA (1) | CA2542478C (enExample) |
| DK (1) | DK1673155T3 (enExample) |
| FR (1) | FR2860796B1 (enExample) |
| WO (1) | WO2005037392A2 (enExample) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5318585B2 (ja) * | 2006-01-24 | 2013-10-16 | サントル、ナショナール、ド、ラ、ルシェルシュ、シアンティフィク、（セーエヌエルエス） | カルシウムの抑制による疾患を治療および／または予防するための薬剤の製造のための高分子量細胞外ヘモグロビンの使用 |
| FR2919785B1 (fr) | 2007-08-09 | 2017-10-20 | Hemarina Sa | Utilisation d'une globine, d'un protomere de globine ou d'une hemoglobine extracellulaire pour la preservation d'organes, de tissus, ou de cellules d'organes ou de tissus, ou de culture de cellules |
| US8273857B2 (en) * | 2009-09-22 | 2012-09-25 | Jen-Chang Hsia | Compositions and methods of use of neurovascular protective multifunctional polynitroxylated pegylated carboxy hemoglobins for transfusion and critical care medicine |
| FR3005663B1 (fr) * | 2013-05-16 | 2016-02-26 | Hemarina | Lyophilisat de ver marin et ses utilisations |
| CL2018002378A1 (es) * | 2018-08-20 | 2018-10-12 | Univ Austral De Chile | Medicamento útil como sustituto sanguineo para tratar, a la vez, la anemia aguda por pedida de sangre y la infección bacteriana en mamíferos |
| WO2022072475A1 (en) * | 2020-09-29 | 2022-04-07 | Hbo2 Therapeutics Llc | Partially oxidized hemoglobin-based oxygen carrier as a treatment for cyanide with or without carbon monoxide poisoning |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4659568A (en) * | 1985-02-27 | 1987-04-21 | American Cyanamid Company | Process for solubilization, purification and characterization of protein from insoluble protein aggregates or complexes and compositions of matter therefrom |
| KR100253916B1 (ko) * | 1997-12-29 | 2000-05-01 | 김충환 | 사람 인슐린 전구체의 제조방법 |
| DE60045005D1 (de) * | 1999-06-28 | 2010-11-04 | Oklahoma Med Res Found | Inhibitoren des memapsin 2 und ihre verwendung |
| FR2809624B1 (fr) * | 2000-05-31 | 2002-08-02 | Centre Nat Rech Scient | Utilisation comme substitut sanguin d'une hemoglobine extracellulaire de poids moleculaire eleve |
-
2003
- 2003-10-14 FR FR0311992A patent/FR2860796B1/fr not_active Expired - Lifetime
-
2004
- 2004-10-13 WO PCT/FR2004/002602 patent/WO2005037392A2/fr not_active Ceased
- 2004-10-13 CA CA2542478A patent/CA2542478C/fr not_active Expired - Lifetime
- 2004-10-13 US US10/575,628 patent/US7776526B2/en active Active
- 2004-10-13 DK DK04817226.6T patent/DK1673155T3/da active
- 2004-10-13 EP EP04817226A patent/EP1673155B1/fr not_active Expired - Lifetime
- 2004-10-13 JP JP2006534789A patent/JP2007508024A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JP2007508024A (ja) | 2007-04-05 |
| FR2860796A1 (fr) | 2005-04-15 |
| WO2005037392A2 (fr) | 2005-04-28 |
| FR2860796B1 (fr) | 2006-02-10 |
| EP1673155A2 (fr) | 2006-06-28 |
| WO2005037392A3 (fr) | 2006-09-14 |
| DK1673155T3 (da) | 2012-11-05 |
| US7776526B2 (en) | 2010-08-17 |
| EP1673155B1 (fr) | 2012-08-22 |
| CA2542478A1 (fr) | 2005-04-28 |
| US20070037160A1 (en) | 2007-02-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |