CA2530994A1 - Transformation de chloroplastes de pourpier - Google Patents
Transformation de chloroplastes de pourpier Download PDFInfo
- Publication number
- CA2530994A1 CA2530994A1 CA002530994A CA2530994A CA2530994A1 CA 2530994 A1 CA2530994 A1 CA 2530994A1 CA 002530994 A CA002530994 A CA 002530994A CA 2530994 A CA2530994 A CA 2530994A CA 2530994 A1 CA2530994 A1 CA 2530994A1
- Authority
- CA
- Canada
- Prior art keywords
- duckweed
- nucleotide sequence
- plant
- nucleic acid
- plastome
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 244000207740 Lemna minor Species 0.000 title claims abstract description 213
- 235000006439 Lemna minor Nutrition 0.000 title claims abstract description 170
- 235000001855 Portulaca oleracea Nutrition 0.000 title claims abstract description 168
- 210000003763 chloroplast Anatomy 0.000 title claims description 55
- 230000009466 transformation Effects 0.000 title abstract description 35
- 210000002706 plastid Anatomy 0.000 claims abstract description 96
- 210000004027 cell Anatomy 0.000 claims abstract description 69
- 230000014509 gene expression Effects 0.000 claims abstract description 68
- 241000196324 Embryophyta Species 0.000 claims abstract description 54
- 238000000034 method Methods 0.000 claims abstract description 50
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 48
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 47
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 47
- 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract description 29
- 239000002773 nucleotide Substances 0.000 claims description 125
- 125000003729 nucleotide group Chemical group 0.000 claims description 125
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 62
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 62
- 229920001184 polypeptide Polymers 0.000 claims description 60
- 230000008685 targeting Effects 0.000 claims description 29
- 239000003550 marker Substances 0.000 claims description 28
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims description 17
- 241000208125 Nicotiana Species 0.000 claims description 16
- 239000012634 fragment Substances 0.000 claims description 15
- 230000006801 homologous recombination Effects 0.000 claims description 11
- 238000002744 homologous recombination Methods 0.000 claims description 11
- 238000004519 manufacturing process Methods 0.000 claims description 11
- 241000209499 Lemna Species 0.000 claims description 10
- 108020004465 16S ribosomal RNA Proteins 0.000 claims description 9
- 101150075980 psbA gene Proteins 0.000 claims description 8
- UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 claims description 8
- 229960000268 spectinomycin Drugs 0.000 claims description 8
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 claims description 8
- 230000001172 regenerating effect Effects 0.000 claims description 7
- 230000005030 transcription termination Effects 0.000 claims description 7
- 108091026890 Coding region Proteins 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 5
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims description 4
- 229940079322 interferon Drugs 0.000 claims description 4
- 229960005322 streptomycin Drugs 0.000 claims description 4
- 230000004083 survival effect Effects 0.000 claims description 4
- 229960005486 vaccine Drugs 0.000 claims description 4
- -1 .beta-glucoronidase Proteins 0.000 claims description 3
- 244000183376 Lemna aequinoctialis Species 0.000 claims description 3
- 230000003115 biocidal effect Effects 0.000 claims description 3
- 101150074945 rbcL gene Proteins 0.000 claims description 3
- 101100301006 Allochromatium vinosum (strain ATCC 17899 / DSM 180 / NBRC 103801 / NCIMB 10441 / D) cbbL2 gene Proteins 0.000 claims description 2
- 108010079709 Angiostatins Proteins 0.000 claims description 2
- 102000012936 Angiostatins Human genes 0.000 claims description 2
- 102100025064 Cellular tumor antigen p53 Human genes 0.000 claims description 2
- 108090000394 Erythropoietin Proteins 0.000 claims description 2
- 102000003951 Erythropoietin Human genes 0.000 claims description 2
- 108010017213 Granulocyte-Macrophage Colony-Stimulating Factor Proteins 0.000 claims description 2
- 102000004457 Granulocyte-Macrophage Colony-Stimulating Factor Human genes 0.000 claims description 2
- 102000018997 Growth Hormone Human genes 0.000 claims description 2
- 108010051696 Growth Hormone Proteins 0.000 claims description 2
- 102100023915 Insulin Human genes 0.000 claims description 2
- 108090001061 Insulin Proteins 0.000 claims description 2
- 244000207747 Lemna gibba Species 0.000 claims description 2
- 108010092277 Leptin Proteins 0.000 claims description 2
- 102000016267 Leptin Human genes 0.000 claims description 2
- 241001465754 Metazoa Species 0.000 claims description 2
- 108010051456 Plasminogen Proteins 0.000 claims description 2
- 102000013566 Plasminogen Human genes 0.000 claims description 2
- 108050002653 Retinoblastoma protein Proteins 0.000 claims description 2
- 102000007562 Serum Albumin Human genes 0.000 claims description 2
- 108010071390 Serum Albumin Proteins 0.000 claims description 2
- 241000339989 Wolffia Species 0.000 claims description 2
- FZCSTZYAHCUGEM-UHFFFAOYSA-N aspergillomarasmine B Natural products OC(=O)CNC(C(O)=O)CNC(C(O)=O)CC(O)=O FZCSTZYAHCUGEM-UHFFFAOYSA-N 0.000 claims description 2
- 101150004101 cbbL gene Proteins 0.000 claims description 2
- 229940105423 erythropoietin Drugs 0.000 claims description 2
- 239000000122 growth hormone Substances 0.000 claims description 2
- 229940125396 insulin Drugs 0.000 claims description 2
- 229940039781 leptin Drugs 0.000 claims description 2
- NRYBAZVQPHGZNS-ZSOCWYAHSA-N leptin Chemical compound O=C([C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](N)CC(C)C)CCSC)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CS)C(O)=O NRYBAZVQPHGZNS-ZSOCWYAHSA-N 0.000 claims description 2
- OXCMYAYHXIHQOA-UHFFFAOYSA-N potassium;[2-butyl-5-chloro-3-[[4-[2-(1,2,4-triaza-3-azanidacyclopenta-1,4-dien-5-yl)phenyl]phenyl]methyl]imidazol-4-yl]methanol Chemical compound [K+].CCCCC1=NC(Cl)=C(CO)N1CC1=CC=C(C=2C(=CC=CC=2)C2=N[N-]N=N2)C=C1 OXCMYAYHXIHQOA-UHFFFAOYSA-N 0.000 claims description 2
- 230000001737 promoting effect Effects 0.000 claims description 2
- 102000004547 Glucosylceramidase Human genes 0.000 claims 1
- 108010017544 Glucosylceramidase Proteins 0.000 claims 1
- 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 claims 1
- 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 claims 1
- 235000006438 Lemna gibba Nutrition 0.000 claims 1
- 241000209501 Spirodela Species 0.000 claims 1
- 108091036066 Three prime untranslated region Proteins 0.000 claims 1
- 239000003242 anti bacterial agent Substances 0.000 claims 1
- 239000000203 mixture Substances 0.000 abstract description 9
- 230000001131 transforming effect Effects 0.000 abstract description 7
- 230000014616 translation Effects 0.000 abstract description 6
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract description 5
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract description 5
- 210000001519 tissue Anatomy 0.000 description 31
- 108090000623 proteins and genes Proteins 0.000 description 30
- 108700019146 Transgenes Proteins 0.000 description 21
- 239000013598 vector Substances 0.000 description 20
- 108020004414 DNA Proteins 0.000 description 17
- 239000002609 medium Substances 0.000 description 11
- HFCYZXMHUIHAQI-UHFFFAOYSA-N Thidiazuron Chemical compound C=1C=CC=CC=1NC(=O)NC1=CN=NS1 HFCYZXMHUIHAQI-UHFFFAOYSA-N 0.000 description 10
- 230000010354 integration Effects 0.000 description 9
- 239000006870 ms-medium Substances 0.000 description 9
- 239000004009 herbicide Substances 0.000 description 8
- 239000002245 particle Substances 0.000 description 8
- 239000000047 product Substances 0.000 description 8
- OVSKIKFHRZPJSS-DOMIDYPGSA-N 2-(2,4-dichlorophenoxy)acetic acid Chemical compound OC(=O)[14CH2]OC1=CC=C(Cl)C=C1Cl OVSKIKFHRZPJSS-DOMIDYPGSA-N 0.000 description 7
- 229920001817 Agar Polymers 0.000 description 7
- 229930006000 Sucrose Natural products 0.000 description 7
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 7
- 108020004566 Transfer RNA Proteins 0.000 description 7
- 239000008272 agar Substances 0.000 description 7
- 229930027917 kanamycin Natural products 0.000 description 7
- 229960000318 kanamycin Drugs 0.000 description 7
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 7
- 229930182823 kanamycin A Natural products 0.000 description 7
- 239000005720 sucrose Substances 0.000 description 7
- 229920002148 Gellan gum Polymers 0.000 description 6
- 235000018102 proteins Nutrition 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- IAJOBQBIJHVGMQ-UHFFFAOYSA-N 2-amino-4-[hydroxy(methyl)phosphoryl]butanoic acid Chemical compound CP(O)(=O)CCC(N)C(O)=O IAJOBQBIJHVGMQ-UHFFFAOYSA-N 0.000 description 5
- 238000010586 diagram Methods 0.000 description 5
- 230000002363 herbicidal effect Effects 0.000 description 5
- 238000012986 modification Methods 0.000 description 5
- 230000004048 modification Effects 0.000 description 5
- 239000013612 plasmid Substances 0.000 description 5
- 241000894007 species Species 0.000 description 5
- 230000035897 transcription Effects 0.000 description 5
- 238000013518 transcription Methods 0.000 description 5
- 238000011426 transformation method Methods 0.000 description 5
- 108010000700 Acetolactate synthase Proteins 0.000 description 4
- 108091029795 Intergenic region Proteins 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- IAJOBQBIJHVGMQ-BYPYZUCNSA-N glufosinate-P Chemical compound CP(O)(=O)CC[C@H](N)C(O)=O IAJOBQBIJHVGMQ-BYPYZUCNSA-N 0.000 description 4
- 230000001939 inductive effect Effects 0.000 description 4
- 230000000442 meristematic effect Effects 0.000 description 4
- 230000000813 microbial effect Effects 0.000 description 4
- 230000006798 recombination Effects 0.000 description 4
- 238000005215 recombination Methods 0.000 description 4
- 108020005345 3' Untranslated Regions Proteins 0.000 description 3
- 108010020183 3-phosphoshikimate 1-carboxyvinyltransferase Proteins 0.000 description 3
- 239000002028 Biomass Substances 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 108700028146 Genetic Enhancer Elements Proteins 0.000 description 3
- 108010025815 Kanamycin Kinase Proteins 0.000 description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- 108020000999 Viral RNA Proteins 0.000 description 3
- 229940126575 aminoglycoside Drugs 0.000 description 3
- 239000012737 fresh medium Substances 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000008929 regeneration Effects 0.000 description 3
- 238000011069 regeneration method Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- OVSKIKFHRZPJSS-UHFFFAOYSA-N 2,4-D Chemical compound OC(=O)COC1=CC=C(Cl)C=C1Cl OVSKIKFHRZPJSS-UHFFFAOYSA-N 0.000 description 2
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 2
- 229940087195 2,4-dichlorophenoxyacetate Drugs 0.000 description 2
- 229930024421 Adenine Natural products 0.000 description 2
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 2
- 102100024085 Alpha-aminoadipic semialdehyde dehydrogenase Human genes 0.000 description 2
- 241000209524 Araceae Species 0.000 description 2
- 108010049668 Betaine-Aldehyde Dehydrogenase Proteins 0.000 description 2
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 2
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 2
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 description 2
- 244000061176 Nicotiana tabacum Species 0.000 description 2
- 102000004316 Oxidoreductases Human genes 0.000 description 2
- 108090000854 Oxidoreductases Proteins 0.000 description 2
- 101150067314 aadA gene Proteins 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 229960000643 adenine Drugs 0.000 description 2
- 101150103518 bar gene Proteins 0.000 description 2
- GINJFDRNADDBIN-FXQIFTODSA-N bilanafos Chemical compound OC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](N)CCP(C)(O)=O GINJFDRNADDBIN-FXQIFTODSA-N 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 244000309464 bull Species 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- 230000034303 cell budding Effects 0.000 description 2
- 238000004520 electroporation Methods 0.000 description 2
- 239000013604 expression vector Substances 0.000 description 2
- XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 239000010931 gold Substances 0.000 description 2
- 229910052737 gold Inorganic materials 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 210000004962 mammalian cell Anatomy 0.000 description 2
- 108010082527 phosphinothricin N-acetyltransferase Proteins 0.000 description 2
- 230000029553 photosynthesis Effects 0.000 description 2
- 238000010672 photosynthesis Methods 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000012552 review Methods 0.000 description 2
- 238000013341 scale-up Methods 0.000 description 2
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
- 238000013519 translation Methods 0.000 description 2
- 230000003442 weekly effect Effects 0.000 description 2
- NDUPDOJHUQKPAG-UHFFFAOYSA-M 2,2-Dichloropropanoate Chemical compound CC(Cl)(Cl)C([O-])=O NDUPDOJHUQKPAG-UHFFFAOYSA-M 0.000 description 1
- UPMXNNIRAGDFEH-UHFFFAOYSA-N 3,5-dibromo-4-hydroxybenzonitrile Chemical compound OC1=C(Br)C=C(C#N)C=C1Br UPMXNNIRAGDFEH-UHFFFAOYSA-N 0.000 description 1
- CAAMSDWKXXPUJR-UHFFFAOYSA-N 3,5-dihydro-4H-imidazol-4-one Chemical compound O=C1CNC=N1 CAAMSDWKXXPUJR-UHFFFAOYSA-N 0.000 description 1
- 108091000044 4-hydroxy-tetrahydrodipicolinate synthase Proteins 0.000 description 1
- 102000000452 Acetyl-CoA carboxylase Human genes 0.000 description 1
- 108010016219 Acetyl-CoA carboxylase Proteins 0.000 description 1
- 241000589158 Agrobacterium Species 0.000 description 1
- 101710154825 Aminoglycoside 3'-phosphotransferase Proteins 0.000 description 1
- 241001167018 Aroa Species 0.000 description 1
- 102000003823 Aromatic-L-amino-acid decarboxylases Human genes 0.000 description 1
- 108090000121 Aromatic-L-amino-acid decarboxylases Proteins 0.000 description 1
- 108010055400 Aspartate kinase Proteins 0.000 description 1
- 241000686404 Australina Species 0.000 description 1
- 229930192334 Auxin Natural products 0.000 description 1
- 108700003860 Bacterial Genes Proteins 0.000 description 1
- 108010006654 Bleomycin Proteins 0.000 description 1
- 239000005489 Bromoxynil Substances 0.000 description 1
- 241000269333 Caudata Species 0.000 description 1
- 108020004638 Circular DNA Proteins 0.000 description 1
- 108020004705 Codon Proteins 0.000 description 1
- 108700010070 Codon Usage Proteins 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 108030005585 Cyanamide hydratases Proteins 0.000 description 1
- 108010075021 Cytochromes f Proteins 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- YAHZABJORDUQGO-NQXXGFSBSA-N D-ribulose 1,5-bisphosphate Chemical compound OP(=O)(O)OC[C@@H](O)[C@@H](O)C(=O)COP(O)(O)=O YAHZABJORDUQGO-NQXXGFSBSA-N 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- 102100024746 Dihydrofolate reductase Human genes 0.000 description 1
- 108700016256 Dihydropteroate synthases Proteins 0.000 description 1
- 101100491986 Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) aromA gene Proteins 0.000 description 1
- 108700039887 Essential Genes Proteins 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- 229930182566 Gentamicin Natural products 0.000 description 1
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 1
- 239000005980 Gibberellic acid Substances 0.000 description 1
- 241001315191 Gladiata Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 239000005562 Glyphosate Substances 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
- FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 1
- 241000339550 Landoltia Species 0.000 description 1
- 240000005010 Landoltia punctata Species 0.000 description 1
- 241000339996 Lemna obscura Species 0.000 description 1
- 241000339991 Lemna tenera Species 0.000 description 1
- 240000000263 Lemna trisulca Species 0.000 description 1
- 241000339987 Lemna valdiviana Species 0.000 description 1
- OJMMVQQUTAEWLP-UHFFFAOYSA-N Lincomycin Natural products CN1CC(CCC)CC1C(=O)NC(C(C)O)C1C(O)C(O)C(O)C(SC)O1 OJMMVQQUTAEWLP-UHFFFAOYSA-N 0.000 description 1
- 241000199616 Lingulata Species 0.000 description 1
- 240000005847 Lysimachia japonica Species 0.000 description 1
- 101150051060 MRPS8 gene Proteins 0.000 description 1
- 102000008109 Mixed Function Oxygenases Human genes 0.000 description 1
- 108010074633 Mixed Function Oxygenases Proteins 0.000 description 1
- 101150109579 Mrps7 gene Proteins 0.000 description 1
- 241001308575 Neglecta Species 0.000 description 1
- 101100363725 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) crp-15 gene Proteins 0.000 description 1
- 108010033272 Nitrilase Proteins 0.000 description 1
- 108091005461 Nucleic proteins Proteins 0.000 description 1
- 108700026244 Open Reading Frames Proteins 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 239000005590 Oxyfluorfen Substances 0.000 description 1
- OQMBBFQZGJFLBU-UHFFFAOYSA-N Oxyfluorfen Chemical compound C1=C([N+]([O-])=O)C(OCC)=CC(OC=2C(=CC(=CC=2)C(F)(F)F)Cl)=C1 OQMBBFQZGJFLBU-UHFFFAOYSA-N 0.000 description 1
- 102000004020 Oxygenases Human genes 0.000 description 1
- 108090000417 Oxygenases Proteins 0.000 description 1
- 101150053185 P450 gene Proteins 0.000 description 1
- 238000010222 PCR analysis Methods 0.000 description 1
- 101150075130 PNOC gene Proteins 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 108010060806 Photosystem II Protein Complex Proteins 0.000 description 1
- 108010039918 Polylysine Proteins 0.000 description 1
- 108010001267 Protein Subunits Proteins 0.000 description 1
- 102000002067 Protein Subunits Human genes 0.000 description 1
- 101710142009 Protein insensitive Proteins 0.000 description 1
- 101150020647 RPS7 gene Proteins 0.000 description 1
- 101100363771 Schizosaccharomyces pombe (strain 972 / ATCC 24843) rps801 gene Proteins 0.000 description 1
- 244000300264 Spinacia oleracea Species 0.000 description 1
- 235000009337 Spinacia oleracea Nutrition 0.000 description 1
- 240000000067 Spirodela polyrhiza Species 0.000 description 1
- 229940100389 Sulfonylurea Drugs 0.000 description 1
- 108010022394 Threonine synthase Proteins 0.000 description 1
- 101100363753 Xenopus laevis rps7 gene Proteins 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 101150008263 accD gene Proteins 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 101150037081 aroA gene Proteins 0.000 description 1
- 101150026213 atpB gene Proteins 0.000 description 1
- 101150035600 atpD gene Proteins 0.000 description 1
- 101150038923 atpF gene Proteins 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- 229960003237 betaine Drugs 0.000 description 1
- SXKNCCSPZDCRFD-UHFFFAOYSA-N betaine aldehyde Chemical compound C[N+](C)(C)CC=O SXKNCCSPZDCRFD-UHFFFAOYSA-N 0.000 description 1
- 108091008324 binding proteins Proteins 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000001486 biosynthesis of amino acids Effects 0.000 description 1
- ZCILODAAHLISPY-UHFFFAOYSA-N biphenyl ether Natural products C1=C(CC=C)C(O)=CC(OC=2C(=CC(CC=C)=CC=2)O)=C1 ZCILODAAHLISPY-UHFFFAOYSA-N 0.000 description 1
- 229960001561 bleomycin Drugs 0.000 description 1
- OYVAGSVQBOHSSS-UAPAGMARSA-O bleomycin A2 Chemical compound N([C@H](C(=O)N[C@H](C)[C@@H](O)[C@H](C)C(=O)N[C@@H]([C@H](O)C)C(=O)NCCC=1SC=C(N=1)C=1SC=C(N=1)C(=O)NCCC[S+](C)C)[C@@H](O[C@H]1[C@H]([C@@H](O)[C@H](O)[C@H](CO)O1)O[C@@H]1[C@H]([C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O1)O)C=1N=CNC=1)C(=O)C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C OYVAGSVQBOHSSS-UAPAGMARSA-O 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000010307 cell transformation Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 229930002868 chlorophyll a Natural products 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 229930002869 chlorophyll b Natural products 0.000 description 1
- NSMUHPMZFPKNMZ-VBYMZDBQSA-M chlorophyll b Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C=O)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 NSMUHPMZFPKNMZ-VBYMZDBQSA-M 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 1
- 239000004062 cytokinin Substances 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 102000004419 dihydrofolate reductase Human genes 0.000 description 1
- 108020001096 dihydrofolate reductase Proteins 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 239000008393 encapsulating agent Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 210000001339 epidermal cell Anatomy 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 1
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical compound C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 description 1
- KWIUHFFTVRNATP-UHFFFAOYSA-N glycine betaine Chemical compound C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 description 1
- 229940097068 glyphosate Drugs 0.000 description 1
- 239000001307 helium Substances 0.000 description 1
- 229910052734 helium Inorganic materials 0.000 description 1
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 description 1
- 108010002685 hygromycin-B kinase Proteins 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 229960000310 isoleucine Drugs 0.000 description 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
- OJMMVQQUTAEWLP-KIDUDLJLSA-N lincomycin Chemical compound CN1C[C@H](CCC)C[C@H]1C(=O)N[C@H]([C@@H](C)O)[C@@H]1[C@H](O)[C@H](O)[C@@H](O)[C@@H](SC)O1 OJMMVQQUTAEWLP-KIDUDLJLSA-N 0.000 description 1
- 229960005287 lincomycin Drugs 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229960000485 methotrexate Drugs 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 239000005648 plant growth regulator Substances 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 229920000656 polylysine Polymers 0.000 description 1
- 230000001323 posttranslational effect Effects 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 210000001938 protoplast Anatomy 0.000 description 1
- 101150065117 psbE gene Proteins 0.000 description 1
- 108700022487 rRNA Genes Proteins 0.000 description 1
- 238000010188 recombinant method Methods 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 101150077391 rps8 gene Proteins 0.000 description 1
- 101150036132 rpsG gene Proteins 0.000 description 1
- 101150094975 rpsH gene Proteins 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 101150090749 sulI gene Proteins 0.000 description 1
- 229940124530 sulfonamide Drugs 0.000 description 1
- 150000003456 sulfonamides Chemical class 0.000 description 1
- YROXIXLRRCOBKF-UHFFFAOYSA-N sulfonylurea Chemical class OC(=N)N=S(=O)=O YROXIXLRRCOBKF-UHFFFAOYSA-N 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 229940113082 thymine Drugs 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 description 1
- 229910052721 tungsten Inorganic materials 0.000 description 1
- 239000010937 tungsten Substances 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
- 108700026220 vif Genes Proteins 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8214—Plastid transformation
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Developmental Biology & Embryology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US48416603P | 2003-07-01 | 2003-07-01 | |
US60/484,166 | 2003-07-01 | ||
US49217903P | 2003-08-01 | 2003-08-01 | |
US60/492,179 | 2003-08-01 | ||
PCT/US2004/021106 WO2005005643A2 (fr) | 2003-07-01 | 2004-06-30 | Transformation de chloroplastes de pourpier |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2530994A1 true CA2530994A1 (fr) | 2005-01-20 |
Family
ID=34068186
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002530994A Abandoned CA2530994A1 (fr) | 2003-07-01 | 2004-06-30 | Transformation de chloroplastes de pourpier |
Country Status (10)
Country | Link |
---|---|
US (1) | US20050044593A1 (fr) |
EP (1) | EP1639114A2 (fr) |
JP (1) | JP2007527218A (fr) |
KR (1) | KR20060107908A (fr) |
AU (1) | AU2004256442A1 (fr) |
CA (1) | CA2530994A1 (fr) |
IL (1) | IL172761A0 (fr) |
MX (1) | MXPA06000092A (fr) |
RU (1) | RU2006102864A (fr) |
WO (1) | WO2005005643A2 (fr) |
Families Citing this family (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2005212431B2 (en) | 2004-02-11 | 2010-07-15 | Synthon Biopharmaceuticals B.V. | Expression of plasminogen and microplasminogen in duckweed |
JP5086999B2 (ja) | 2005-09-16 | 2012-11-28 | バイエル・クロップサイエンス・アーゲー | 内腔指向性タンパク質を発現する色素体形質転換植物 |
US7622573B2 (en) * | 2006-01-17 | 2009-11-24 | Biolex, Inc. | Expression control elements from the lemnaceae family |
JP2008171800A (ja) | 2006-10-31 | 2008-07-24 | Fei Co | 荷電粒子ビーム処理用保護層 |
AU2008308486B2 (en) * | 2007-10-05 | 2014-08-07 | Corteva Agriscience Llc | Methods for transferring molecular substances into plant cells |
WO2009114537A1 (fr) | 2008-03-10 | 2009-09-17 | Monsanto Technology Llc | Molécules chimériques promotrices pour l’expression génétique chez les procaryotes |
AU2009324539B2 (en) | 2008-12-10 | 2015-10-01 | Synthetic Genomics, Inc. | Production of branched-chain alcohols by photosynthetic microorganisms |
EP2417262B1 (fr) * | 2009-04-07 | 2015-05-20 | Dow AgroSciences LLC | Distribution à médiation par nanoparticules de nucléases à spécificité de séquence |
US8956834B2 (en) | 2009-06-29 | 2015-02-17 | Synthetic Genomics, Inc. | Acyl-ACP thioesterase genes and uses therefor |
EP2464722B1 (fr) | 2009-08-11 | 2017-10-04 | Synthetic Genomics, Inc. | Production microbienne d'alcools gras |
NZ598419A (en) * | 2009-10-16 | 2013-11-29 | Dow Agrosciences Llc | Use of dendrimer nanotechnology for delivery of biomolecules into plant cells |
AU2011238826B2 (en) * | 2010-03-31 | 2014-11-13 | Corteva Agriscience Llc | Plant peptide gamma-zein for delivery of biomolecules into plant cells |
US8530207B2 (en) | 2010-12-23 | 2013-09-10 | Exxonmobil Research And Engineering Company | Photosynthetic microorganisms comprising exogenous prokaryotic acyl-ACP thioesterases and methods for producing fatty acids |
ES2665516T3 (es) | 2010-12-23 | 2018-04-26 | Exxonmobil Research And Engineering Company | Microorganismos modificados genéticamente que comprenden 4-hidroxibenzoíl-CoA-tioesterasas, y métodos de uso de los mismos para producir ácidos grasos libres, derivados de ácidos grasos y 4-hidroxibenzoíl-CoA-tioesterasas |
WO2012087676A2 (fr) | 2010-12-23 | 2012-06-28 | Exxonmobil Research And Enginnering Company | Production d'acides gras et de dérivés d'acides gras par des microorganismes recombinés exprimant des polypeptides présentant une activité lipolytique |
WO2012092033A1 (fr) | 2010-12-31 | 2012-07-05 | Exxonmobil Research And Engineering Company | Amélioration du procédé de production de biomasse par perturbation des voies de dissipation de l'énergie lumineuse |
WO2012129443A2 (fr) * | 2011-03-23 | 2012-09-27 | Dow Agrosciences Llc | Conjugués peptidiques supports de points quantiques adaptés pour des applications d'imagerie et d'administration dans des plantes |
US9096834B2 (en) | 2012-02-24 | 2015-08-04 | Exxonmobil Research And Engineering Company | Recombinant microorganisms comprising thioesterase and lysophosphatidic acid acyltransferase genes for fatty acid production |
US9181568B2 (en) | 2012-04-23 | 2015-11-10 | Exxonmobil Research And Engineering Company | Cell systems and methods for improving fatty acid synthesis by expression of dehydrogenases |
EP2909304A4 (fr) | 2012-10-16 | 2016-09-21 | Exxonmobil Res & Eng Co | Gènes de dgat et procédés d'utilisation pour la production de triglycéride dans des microorganismes recombinés |
ES2740648T3 (es) | 2012-12-06 | 2020-02-06 | Synthetic Genomics Inc | Mutantes de algas que tienen un fenotipo aclimatado a alta luz bloqueado |
BR112015012811A2 (pt) | 2012-12-06 | 2017-11-14 | Exxonmobil Res & Eng Co | genes dgat compreendendo domínios de homologia de plecstrina e uso em micro-organismos recombinantes |
CN103667328B (zh) * | 2013-12-03 | 2017-01-18 | 中国海洋大学 | 一种条斑紫菜质体遗传转化载体的构建方法 |
US20170267984A1 (en) | 2016-03-18 | 2017-09-21 | Exxonmobil Research And Engineering Company | Chlorophyllase overproduction to enhance photosynthetic efficiency |
Family Cites Families (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5932479A (en) * | 1988-09-26 | 1999-08-03 | Auburn University | Genetic engineering of plant chloroplasts |
US5693507A (en) * | 1988-09-26 | 1997-12-02 | Auburn University | Genetic engineering of plant chloroplasts |
US5877402A (en) * | 1990-05-01 | 1999-03-02 | Rutgers, The State University Of New Jersey | DNA constructs and methods for stably transforming plastids of multicellular plants and expressing recombinant proteins therein |
US5451513A (en) * | 1990-05-01 | 1995-09-19 | The State University of New Jersey Rutgers | Method for stably transforming plastids of multicellular plants |
US5576198A (en) * | 1993-12-14 | 1996-11-19 | Calgene, Inc. | Controlled expression of transgenic constructs in plant plastids |
US5545817A (en) * | 1994-03-11 | 1996-08-13 | Calgene, Inc. | Enhanced expression in a plant plastid |
JP2000506019A (ja) * | 1996-03-06 | 2000-05-23 | ラトガーズ・ユニバーシティ | アラビドプシス・サリアナアにおけるプラスチド形質転換 |
US6239332B1 (en) * | 1996-12-05 | 2001-05-29 | Queen's University At Kingston | Constructs and methods for enhancing protein levels in photosynthetic organisms |
US7161064B2 (en) * | 1997-08-12 | 2007-01-09 | North Carolina State University | Method for producing stably transformed duckweed using microprojectile bombardment |
US6040498A (en) * | 1998-08-11 | 2000-03-21 | North Caroline State University | Genetically engineered duckweed |
US6271444B1 (en) * | 1998-07-10 | 2001-08-07 | Calgene Llc | Enhancer elements for increased translation in plant plastids |
US6512162B2 (en) * | 1998-07-10 | 2003-01-28 | Calgene Llc | Expression of eukaryotic peptides in plant plastids |
US6541682B1 (en) * | 1998-11-12 | 2003-04-01 | Calgene Llc | Plastid transformation of solanaceous plants |
US20030041353A1 (en) * | 2001-04-18 | 2003-02-27 | Henry Daniell | Mutiple gene expression for engineering novel pathways and hyperexpression of foreign proteins in plants |
WO2001081605A2 (fr) * | 2000-04-26 | 2001-11-01 | Monsanto Technology Llc | Procede de transformation des plastes de cellules vegetales |
US20030113921A1 (en) * | 2001-07-20 | 2003-06-19 | Gilbertson Larry A. | Nucleic acid sequences capable of improving homologous recombination in plants and plant plastids |
FR2848064B1 (fr) * | 2002-12-06 | 2006-01-13 | Bayer Cropscience Sa | Plantes legumineuses transplastomiques fertiles |
-
2004
- 2004-06-30 AU AU2004256442A patent/AU2004256442A1/en not_active Abandoned
- 2004-06-30 EP EP04756479A patent/EP1639114A2/fr not_active Withdrawn
- 2004-06-30 KR KR1020057025499A patent/KR20060107908A/ko not_active Application Discontinuation
- 2004-06-30 CA CA002530994A patent/CA2530994A1/fr not_active Abandoned
- 2004-06-30 MX MXPA06000092A patent/MXPA06000092A/es not_active Application Discontinuation
- 2004-06-30 JP JP2006518748A patent/JP2007527218A/ja not_active Withdrawn
- 2004-06-30 WO PCT/US2004/021106 patent/WO2005005643A2/fr active Application Filing
- 2004-06-30 RU RU2006102864/13A patent/RU2006102864A/ru not_active Application Discontinuation
- 2004-06-30 US US10/881,813 patent/US20050044593A1/en not_active Abandoned
-
2005
- 2005-12-22 IL IL172761A patent/IL172761A0/en unknown
Also Published As
Publication number | Publication date |
---|---|
JP2007527218A (ja) | 2007-09-27 |
EP1639114A2 (fr) | 2006-03-29 |
WO2005005643A2 (fr) | 2005-01-20 |
RU2006102864A (ru) | 2006-06-10 |
MXPA06000092A (es) | 2006-04-07 |
US20050044593A1 (en) | 2005-02-24 |
IL172761A0 (en) | 2006-04-10 |
WO2005005643A3 (fr) | 2005-09-15 |
KR20060107908A (ko) | 2006-10-16 |
AU2004256442A1 (en) | 2005-01-20 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US20050044593A1 (en) | Chloroplast transformation of duckweed | |
US6040498A (en) | Genetically engineered duckweed | |
US20050060776A1 (en) | Expression of biologically active polypeptides in duckweed | |
JP2009148286A (ja) | 遺伝子工学的に作製されるウキクサ | |
US20070044177A1 (en) | Genetically engineered duckweed | |
AU729122B2 (en) | Methods for improving transformation efficiency | |
CA2540559C (fr) | Expression de polypeptides biologiquement actifs dans du pourpier | |
AU2013302947A1 (en) | Use of a maize untranslated region for transgene expression in plants | |
AU2002257341B2 (en) | Use of duckweed in high throughput screening | |
Ahmad et al. | Green factories: plastids for the production of foreign proteins at high levels | |
AU2002257341A1 (en) | Use of duckweed in high throughput screening | |
JP2009523431A (ja) | ウキクサ科由来の発現制御因子 | |
JP4740567B2 (ja) | キク科植物の葉緑体の形質転換方法 | |
Staub | Expression of recombinant proteins via the plastid genome | |
Beeramganti et al. | Chloroplast expression vector system & its transformation | |
AU2008201438B2 (en) | Genetically engineered duckweed |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FZDE | Discontinued |