CA2498951C - Method for enriching procaryotic dna - Google Patents

Method for enriching procaryotic dna Download PDF

Info

Publication number
CA2498951C
CA2498951C CA2498951A CA2498951A CA2498951C CA 2498951 C CA2498951 C CA 2498951C CA 2498951 A CA2498951 A CA 2498951A CA 2498951 A CA2498951 A CA 2498951A CA 2498951 C CA2498951 C CA 2498951C
Authority
CA
Canada
Prior art keywords
dna
procaryotic
polypeptide
protein
procaryotic dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2498951A
Other languages
English (en)
French (fr)
Other versions
CA2498951A1 (en
Inventor
Karl-Hermann Schmidt
Eberhard Straube
Stefan Russwurm
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SIRS Lab GmbH
Original Assignee
SIRS Lab GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SIRS Lab GmbH filed Critical SIRS Lab GmbH
Publication of CA2498951A1 publication Critical patent/CA2498951A1/en
Application granted granted Critical
Publication of CA2498951C publication Critical patent/CA2498951C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2498951A 2002-09-18 2003-08-08 Method for enriching procaryotic dna Expired - Fee Related CA2498951C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP02020904.5 2002-09-18
EP02020904A EP1400589B1 (de) 2002-09-18 2002-09-18 Verfahren zum Nachweis prokaryontischer DNA
PCT/EP2003/008825 WO2004033683A1 (de) 2002-09-18 2003-08-08 Verfahren zur anreicherung prokaryontischer dna

Publications (2)

Publication Number Publication Date
CA2498951A1 CA2498951A1 (en) 2004-04-22
CA2498951C true CA2498951C (en) 2013-04-23

Family

ID=31896878

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2498951A Expired - Fee Related CA2498951C (en) 2002-09-18 2003-08-08 Method for enriching procaryotic dna

Country Status (12)

Country Link
US (2) US8062854B2 (https=)
EP (1) EP1400589B1 (https=)
JP (1) JP4447459B2 (https=)
KR (1) KR20050057152A (https=)
CN (1) CN1681925A (https=)
AT (1) ATE335816T1 (https=)
AU (1) AU2003260392A1 (https=)
CA (1) CA2498951C (https=)
DE (1) DE50207801D1 (https=)
DK (1) DK1400589T3 (https=)
ES (1) ES2266374T3 (https=)
WO (1) WO2004033683A1 (https=)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1400589T3 (da) 2002-09-18 2006-11-27 Sirs Lab Gmbh Fremgangsmåde til påvisning af prokaryotisk DNA
DE102005001889B4 (de) * 2005-01-14 2012-03-29 Sirs-Lab Gmbh Verfahren zur Steigerung der Bindungskapazität und -effizienz eines Proteins, das nicht methylierte CPG-Motive enthaltende DNA spezifisch bindet
ES2384571T3 (es) * 2004-03-05 2012-07-09 Sirs-Lab Gmbh Procedimiento para el enriquecimiento y/o la separación de ADN procariota mediante una proteína que se une específicamente a ADN que contiene motivos CpG no metilados
GB0413688D0 (en) * 2004-06-18 2004-07-21 Novartis Forschungsstiftung Analysis of methylated nucleic acid
DE202005009490U1 (de) * 2005-06-16 2006-10-19 Sirs-Lab Gmbh Vorrichtung zur Anreicherung/Abtrennung von nicht-methylierte CpG-Motive enthaltender DNA
DE102005034628B4 (de) * 2005-07-19 2007-08-23 Epigenomics Ag Verfahren zur Untersuchung von Cytosin-Methylierungen in DNA
DE102007041864B4 (de) 2007-09-04 2012-05-03 Sirs-Lab Gmbh Verfahren zum Nachweis von Bakterien und Pilzen
DK3067431T3 (da) 2010-09-15 2019-10-14 Debiopharm Int Sa Indretning omfattende blodkomponenter til at adskille målmolekyler eller partikler fra prøver
US8980553B2 (en) * 2011-04-02 2015-03-17 New England Biolabs, Inc. Methods and compositions for enriching either target polynucleotides or non-target polynucleotides from a mixture of target and non-target polynucleotides
US9145580B2 (en) * 2011-04-02 2015-09-29 New England Biolabs, Inc. Methods and compositions for enriching either target polynucleotides or non-target polynucleotides from a mixture of target and non-target polynucleotides
DE102012219142B4 (de) 2012-10-19 2016-02-25 Analytik Jena Ag Verfahren zum Trennen, Erfassen oder Anreichern von unterschiedlichen DNA-Spezies
US12612615B2 (en) 2019-04-26 2026-04-28 Thermo Fisher Scientific Baltics, UAB Isolated nucleic acid binding domains

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6239116B1 (en) 1994-07-15 2001-05-29 University Of Iowa Research Foundation Immunostimulatory nucleic acid molecules
WO1997005484A1 (en) 1995-07-28 1997-02-13 Beth Israel Deaconess Medical Center, Inc. A method of and test kit for mutagenesis testing
EP1248798A2 (en) 1999-08-18 2002-10-16 Fraunhofer-Gesellschaft Zur Förderung Der Angewandten Forschung E.V. Human dna sequences
JP2002034565A (ja) 2000-07-19 2002-02-05 Japan Science & Technology Corp 細菌dnaを特異的に認識する受容体タンパク質
DK1400589T3 (da) 2002-09-18 2006-11-27 Sirs Lab Gmbh Fremgangsmåde til påvisning af prokaryotisk DNA
ES2384571T3 (es) 2004-03-05 2012-07-09 Sirs-Lab Gmbh Procedimiento para el enriquecimiento y/o la separación de ADN procariota mediante una proteína que se une específicamente a ADN que contiene motivos CpG no metilados
GB0423991D0 (en) 2004-10-29 2004-12-01 Univ Edinburgh Applications of nucleic acid fragments

Also Published As

Publication number Publication date
EP1400589A1 (de) 2004-03-24
JP4447459B2 (ja) 2010-04-07
US20120040363A1 (en) 2012-02-16
ATE335816T1 (de) 2006-09-15
WO2004033683A1 (de) 2004-04-22
DK1400589T3 (da) 2006-11-27
DE50207801D1 (de) 2006-09-21
JP2005538737A (ja) 2005-12-22
KR20050057152A (ko) 2005-06-16
ES2266374T3 (es) 2007-03-01
US8062854B2 (en) 2011-11-22
CN1681925A (zh) 2005-10-12
CA2498951A1 (en) 2004-04-22
EP1400589B1 (de) 2006-08-09
US8288115B2 (en) 2012-10-16
AU2003260392A1 (en) 2004-05-04
US20100316993A1 (en) 2010-12-16

Similar Documents

Publication Publication Date Title
US8288115B2 (en) Method for enriching a prokaryotic DNA
CN1187362C (zh) 核酸的固相分离法
EP2505666B1 (en) Methods and compositions for enriching either target polynucleotides or non-target polynucleotides from a mixture of target and non-target polynucleotides
US20080305528A1 (en) Isolation of nucleic acids
JP2004501054A (ja) 核酸の単離
US20150159207A1 (en) Use of markers including nucleotide sequence based codes to monitor methods of detection and identification of genetic material
WO1999054506A1 (en) Method of isolating target specific oligonucleotide ligands
CN103695419B (zh) 一种病毒核酸提取试剂
CN101268189A (zh) 分离核酸的方法
CA2558515A1 (en) Method of enriching and/or separating prokaryotic dna by means of a protein which specifically binds dna containing non-methylated cpg motifs
Blackwell [41] Selection of protein binding sites from random nucleic acid sequences
CN111944874B (zh) 一种筛选鉴定胁迫应答基因表达调控因子的方法
US11345959B2 (en) Method for exploring useful genetic resources through bulk metagenome analysis and use thereof
WO1996013608A3 (en) Polynucleic acid sequences for use in the detection and differentiation of prokaryotic organisms
Kapustin et al. High-throughput method of one-step DNA isolation for PCR diagnostics of Mycobacterium tuberculosis
CN115927244A (zh) 一种基于微流控技术的单细胞ChIP-seq文库的构建方法
CN111363748A (zh) 一种核酸适配体及其构建方法和其在检测中华鳖彩虹病毒中的应用
CN114561380B (zh) 细菌核酸提取检测试剂、试剂盒及其方法和应用
CN119592714A (zh) 细菌16s扩增试剂盒、检测方法及应用
Matsumura et al. SuperSAGE as an analytical tool for host and viral gene expression
WO2004056995A1 (en) Process for producing an in vitro peptide expression library
CN113025731A (zh) 一种基于靶向扩增子富集策略的高通量病原菌检测试剂盒
Uhlén et al. Affinity separation of nucleic acids on monosized magnetic beads
Lew et al. [37] Affinity selection of polymerase chain reaction products by DNA-binding proteins
KR20170000804A (ko) 대용량 메타지놈 분석을 통한 유용 유전자원 탐색 방법 및 이의 이용

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed

Effective date: 20160808