CA2476177C - Procede de purification d'erythrocytes par separation et diafiltration - Google Patents
Procede de purification d'erythrocytes par separation et diafiltration Download PDFInfo
- Publication number
- CA2476177C CA2476177C CA2476177A CA2476177A CA2476177C CA 2476177 C CA2476177 C CA 2476177C CA 2476177 A CA2476177 A CA 2476177A CA 2476177 A CA2476177 A CA 2476177A CA 2476177 C CA2476177 C CA 2476177C
- Authority
- CA
- Canada
- Prior art keywords
- red blood
- blood
- blood cells
- whole blood
- cell fraction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0641—Erythrocytes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/14—Blood; Artificial blood
- A61K35/16—Blood plasma; Blood serum
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/41—Porphyrin- or corrin-ring-containing peptides
- A61K38/42—Haemoglobins; Myoglobins
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3692—Washing or rinsing blood or blood constituents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3693—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging
- A61M1/3696—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/08—Plasma substitutes; Perfusion solutions; Dialytics or haemodialytics; Drugs for electrolytic or acid-base disorders, e.g. hypovolemic shock
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3693—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M2202/00—Special media to be introduced, removed or treated
- A61M2202/04—Liquids
- A61M2202/0413—Blood
- A61M2202/0429—Red blood cells; Erythrocytes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- General Health & Medical Sciences (AREA)
- Vascular Medicine (AREA)
- Heart & Thoracic Surgery (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Cardiology (AREA)
- Anesthesiology (AREA)
- Cell Biology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Epidemiology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Diabetes (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Developmental Biology & Embryology (AREA)
- Virology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- External Artificial Organs (AREA)
Abstract
L'invention concerne un procédé de purification d'érythrocytes, consistant à séparer du sang total par centrifugation, par exemple, pour obtenir une fraction d'érythrocytes et une fraction liquide. Le sang total peut être défibriné ou traité pour éviter qu'il y ait coagulation avant la séparation. De préférence, le sang total est du sang de bovin. Le procédé consiste ensuite à soumettre la fraction d'érythrocytes à une diafiltration pour purifier les érythrocytes. Les érythrocytes purifiés peuvent ensuite être lysés pour former un lysat d'érythrocytes purifiés. Les érythrocytes purifiés et le lysat d'érythrocytes purifiés peuvent être utilisés pour produire un succédané d'hémoglobine.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/US2002/006799 WO2003074077A1 (fr) | 2002-02-28 | 2002-02-28 | Procede de purification d'erythrocytes par separation et diafiltration |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2476177A1 CA2476177A1 (fr) | 2003-09-12 |
CA2476177C true CA2476177C (fr) | 2011-06-14 |
Family
ID=27787374
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2476177A Expired - Fee Related CA2476177C (fr) | 2002-02-28 | 2002-02-28 | Procede de purification d'erythrocytes par separation et diafiltration |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP1478388A1 (fr) |
JP (1) | JP2006500317A (fr) |
CN (1) | CN100348267C (fr) |
AU (1) | AU2002245600B2 (fr) |
CA (1) | CA2476177C (fr) |
WO (1) | WO2003074077A1 (fr) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
MX336273B (es) * | 2009-05-14 | 2016-01-13 | Biotechnology Inst I Mas D Sl | Metodo para la preparacion de al menos un compuesto a partir de sangre, y dispositivo de extraccion para ser utilizado en la ejecucion de dicho metodo. |
CA2937484C (fr) * | 2014-01-20 | 2023-09-19 | Halcyon Biomedical, Incorporated | Separation et concentration de particules |
EP3110467B1 (fr) | 2014-02-25 | 2019-07-10 | Biomet Biologics, LLC | Système de séparation par filtre de cellules |
GB201421013D0 (en) * | 2014-11-26 | 2015-01-07 | Turzi Antoine | New standardizations & medical devices for the preparation of platelet rich plasma (PRP) or bone marrow centrate (BMC) |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5854209A (en) | 1995-03-23 | 1998-12-29 | Biopure Corporation | Method for oxygenating tissue having reduced red blood cell flow |
CA1312009C (fr) | 1986-11-10 | 1992-12-29 | Carl W. Rausch | Substitut sanguin semi-synthetique extra pur |
US5084558A (en) | 1987-10-13 | 1992-01-28 | Biopure Corporation | Extra pure semi-synthetic blood substitute |
EP0804244A1 (fr) * | 1993-03-26 | 1997-11-05 | Biorelease Technologies, Inc. | Compositions comportant des proteines heminiques et methodes associees |
US5691452A (en) | 1995-03-23 | 1997-11-25 | Biopure Corporation | Method for preserving a hemoglobin blood substitute |
US5895810A (en) | 1995-03-23 | 1999-04-20 | Biopure Corporation | Stable polymerized hemoglobin and use thereof |
EP1211261A3 (fr) * | 1995-03-23 | 2004-03-17 | Biopure Corporation | Succédané de sang à base d' hémoglobine polymérisée |
US5691453A (en) | 1995-06-07 | 1997-11-25 | Biopure Corporation | Separation of polymerized hemoglobin from unpolymerized hemoglobin on hydroxyapatite using HPLC |
US6518010B2 (en) * | 2001-02-28 | 2003-02-11 | Biopure Corporation | Use of defibrinated blood for manufacture of a hemoglobin-based oxygen carrier |
-
2002
- 2002-02-28 EP EP02713771A patent/EP1478388A1/fr not_active Withdrawn
- 2002-02-28 CN CNB028283376A patent/CN100348267C/zh not_active Expired - Fee Related
- 2002-02-28 AU AU2002245600A patent/AU2002245600B2/en not_active Ceased
- 2002-02-28 JP JP2003572593A patent/JP2006500317A/ja active Pending
- 2002-02-28 CA CA2476177A patent/CA2476177C/fr not_active Expired - Fee Related
- 2002-02-28 WO PCT/US2002/006799 patent/WO2003074077A1/fr active Application Filing
Also Published As
Publication number | Publication date |
---|---|
EP1478388A1 (fr) | 2004-11-24 |
AU2002245600B2 (en) | 2006-12-21 |
JP2006500317A (ja) | 2006-01-05 |
AU2002245600A1 (en) | 2003-09-16 |
CN100348267C (zh) | 2007-11-14 |
CA2476177A1 (fr) | 2003-09-12 |
CN1622823A (zh) | 2005-06-01 |
WO2003074077A1 (fr) | 2003-09-12 |
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CA2476177C (fr) | Procede de purification d'erythrocytes par separation et diafiltration | |
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WO2006108047A2 (fr) | Solutions oxygenees d'hemoglobine polymerisee et leurs utilisations pour la visualisation de tissus oxygenated polymerized hemoglobin solutions and their uses for tissue visualization | |
CZ299357B6 (cs) | Vodný roztok pyridoxylovaného zpolymerovaného hemoglobinu a zpusoby jeho prípravy | |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKLA | Lapsed |
Effective date: 20150302 |