CA2473741A1 - Methode de generation de cellules genetiquement modifiees pour la regulation et l'analyse de genes specifiques d'un locus - Google Patents

Methode de generation de cellules genetiquement modifiees pour la regulation et l'analyse de genes specifiques d'un locus Download PDF

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Publication number
CA2473741A1
CA2473741A1 CA002473741A CA2473741A CA2473741A1 CA 2473741 A1 CA2473741 A1 CA 2473741A1 CA 002473741 A CA002473741 A CA 002473741A CA 2473741 A CA2473741 A CA 2473741A CA 2473741 A1 CA2473741 A1 CA 2473741A1
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CA
Canada
Prior art keywords
leu
glu
ser
lys
cell
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Granted
Application number
CA002473741A
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English (en)
Other versions
CA2473741C (fr
Inventor
Luigi Grasso
J. Bradford Kline
Nicholas C. Nicolaides
Philip M. Sass
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Eisai Inc
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Individual
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Publication of CA2473741A1 publication Critical patent/CA2473741A1/fr
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Publication of CA2473741C publication Critical patent/CA2473741C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2473741A 2002-01-18 2003-01-17 Methode de generation de cellules genetiquement modifiees pour la regulation et l'analyse de genes specifiques d'un locus Expired - Lifetime CA2473741C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US34956502P 2002-01-18 2002-01-18
US60/349,565 2002-01-18
PCT/US2003/001361 WO2003062435A1 (fr) 2002-01-18 2003-01-17 Methode de generation de cellules genetiquement modifiees pour la regulation et l'analyse de genes specifiques d'un locus

Publications (2)

Publication Number Publication Date
CA2473741A1 true CA2473741A1 (fr) 2003-07-31
CA2473741C CA2473741C (fr) 2015-12-22

Family

ID=27613291

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2473741A Expired - Lifetime CA2473741C (fr) 2002-01-18 2003-01-17 Methode de generation de cellules genetiquement modifiees pour la regulation et l'analyse de genes specifiques d'un locus

Country Status (6)

Country Link
US (1) US7638334B2 (fr)
EP (1) EP1474522B1 (fr)
AT (1) ATE548461T1 (fr)
AU (1) AU2003205173C1 (fr)
CA (1) CA2473741C (fr)
WO (1) WO2003062435A1 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6982169B2 (en) * 2001-01-15 2006-01-03 Morphotek, Inc. Chemical inhibitors of mismatch repair
JP4555089B2 (ja) * 2002-11-15 2010-09-29 モーフオテク・インコーポレーテツド ｉｎｖｉｔｒｏ免疫感作により創製されたハイブリドーマからの抗体の高生産量の生成方法
WO2011058555A1 (fr) * 2009-11-12 2011-05-19 Yeda Research And Development Co. Ltd. Procédé d'édition de l'adn dans une cellule et constructions ayant cette capacité
US20150307876A1 (en) * 2012-02-10 2015-10-29 Regents Of The University Of Minnesota Dna assimilation

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2641793B1 (fr) 1988-12-26 1993-10-01 Setratech Procede de recombinaison in vivo de sequences d'adn presentant des mesappariements de bases
US5272071A (en) * 1989-12-22 1993-12-21 Applied Research Systems Ars Holding N.V. Method for the modification of the expression characteristics of an endogenous gene of a given cell line
US5922601A (en) * 1995-01-19 1999-07-13 Biotransplant, Inc. High efficiency gene trap selection of regulated genetic loci
US5681744A (en) * 1995-03-17 1997-10-28 Greenstein; Robert J. Delivery and expression of heterologus genes using upstream enhancer regions of mammalian gene promoters
CA2263958C (fr) 1995-07-26 2012-06-19 Mixis France Recombinaison homologue dans des cellules eucaryotes a systeme de reparation des mesappariements inactive
ATE245194T1 (de) * 1996-10-01 2003-08-15 Geron Corp Katalytische untereinheit der menschlichen telomerase
JP4139561B2 (ja) * 1997-12-05 2008-08-27 ユーロペーイシェ ラボラトリウム フュール モレキュラーバイオロジー（イーエムビーエル） 新規ｄｎａクローニング方法
US6146894A (en) 1998-04-14 2000-11-14 The Johns Hopkins University Method for generating hypermutable organisms
US6355412B1 (en) 1999-07-09 2002-03-12 The European Molecular Biology Laboratory Methods and compositions for directed cloning and subcloning using homologous recombination
US7026119B2 (en) * 2000-02-11 2006-04-11 Morphotek, Inc. Methods for generating hypermutable microbes
WO2001061012A1 (fr) 2000-02-18 2001-08-23 Nicolaides Nicholas C Procede de generation de plantes tres sujettes aux mutations
AU2001238558A1 (en) * 2000-02-23 2001-09-03 Luigi Grasso Methods for generating hypermutable yeast
US6569681B1 (en) 2000-03-14 2003-05-27 Transkaryotic Therapies, Inc. Methods of improving homologous recombination
US6596541B2 (en) * 2000-10-31 2003-07-22 Regeneron Pharmaceuticals, Inc. Methods of modifying eukaryotic cells
ATE501635T1 (de) 2001-01-15 2011-04-15 Morphotek Inc Chemische hemmstoffe des mismatch-repair
JP2005531291A (ja) 2002-02-21 2005-10-20 モーフオテク・インコーポレーテツド Ｐｍｓｒ相同体を使用する超変異性細胞の作成方法

Also Published As

Publication number Publication date
WO2003062435A1 (fr) 2003-07-31
AU2003205173B2 (en) 2008-05-22
CA2473741C (fr) 2015-12-22
AU2003205173C1 (en) 2009-01-29
EP1474522B1 (fr) 2012-03-07
US20030176386A1 (en) 2003-09-18
EP1474522A1 (fr) 2004-11-10
EP1474522A4 (fr) 2005-09-07
US7638334B2 (en) 2009-12-29
ATE548461T1 (de) 2012-03-15

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Effective date: 20230117