CA2473741A1 - Methode de generation de cellules genetiquement modifiees pour la regulation et l'analyse de genes specifiques d'un locus - Google Patents
Methode de generation de cellules genetiquement modifiees pour la regulation et l'analyse de genes specifiques d'un locus Download PDFInfo
- Publication number
- CA2473741A1 CA2473741A1 CA002473741A CA2473741A CA2473741A1 CA 2473741 A1 CA2473741 A1 CA 2473741A1 CA 002473741 A CA002473741 A CA 002473741A CA 2473741 A CA2473741 A CA 2473741A CA 2473741 A1 CA2473741 A1 CA 2473741A1
- Authority
- CA
- Canada
- Prior art keywords
- leu
- glu
- ser
- lys
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US34956502P | 2002-01-18 | 2002-01-18 | |
US60/349,565 | 2002-01-18 | ||
PCT/US2003/001361 WO2003062435A1 (fr) | 2002-01-18 | 2003-01-17 | Methode de generation de cellules genetiquement modifiees pour la regulation et l'analyse de genes specifiques d'un locus |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2473741A1 true CA2473741A1 (fr) | 2003-07-31 |
CA2473741C CA2473741C (fr) | 2015-12-22 |
Family
ID=27613291
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2473741A Expired - Lifetime CA2473741C (fr) | 2002-01-18 | 2003-01-17 | Methode de generation de cellules genetiquement modifiees pour la regulation et l'analyse de genes specifiques d'un locus |
Country Status (6)
Country | Link |
---|---|
US (1) | US7638334B2 (fr) |
EP (1) | EP1474522B1 (fr) |
AT (1) | ATE548461T1 (fr) |
AU (1) | AU2003205173C1 (fr) |
CA (1) | CA2473741C (fr) |
WO (1) | WO2003062435A1 (fr) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6982169B2 (en) * | 2001-01-15 | 2006-01-03 | Morphotek, Inc. | Chemical inhibitors of mismatch repair |
JP4555089B2 (ja) * | 2002-11-15 | 2010-09-29 | モーフオテク・インコーポレーテツド | invitro免疫感作により創製されたハイブリドーマからの抗体の高生産量の生成方法 |
WO2011058555A1 (fr) * | 2009-11-12 | 2011-05-19 | Yeda Research And Development Co. Ltd. | Procédé d'édition de l'adn dans une cellule et constructions ayant cette capacité |
US20150307876A1 (en) * | 2012-02-10 | 2015-10-29 | Regents Of The University Of Minnesota | Dna assimilation |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2641793B1 (fr) | 1988-12-26 | 1993-10-01 | Setratech | Procede de recombinaison in vivo de sequences d'adn presentant des mesappariements de bases |
US5272071A (en) * | 1989-12-22 | 1993-12-21 | Applied Research Systems Ars Holding N.V. | Method for the modification of the expression characteristics of an endogenous gene of a given cell line |
US5922601A (en) * | 1995-01-19 | 1999-07-13 | Biotransplant, Inc. | High efficiency gene trap selection of regulated genetic loci |
US5681744A (en) * | 1995-03-17 | 1997-10-28 | Greenstein; Robert J. | Delivery and expression of heterologus genes using upstream enhancer regions of mammalian gene promoters |
CA2263958C (fr) | 1995-07-26 | 2012-06-19 | Mixis France | Recombinaison homologue dans des cellules eucaryotes a systeme de reparation des mesappariements inactive |
ATE245194T1 (de) * | 1996-10-01 | 2003-08-15 | Geron Corp | Katalytische untereinheit der menschlichen telomerase |
JP4139561B2 (ja) * | 1997-12-05 | 2008-08-27 | ユーロペーイシェ ラボラトリウム フュール モレキュラーバイオロジー(イーエムビーエル) | 新規dnaクローニング方法 |
US6146894A (en) | 1998-04-14 | 2000-11-14 | The Johns Hopkins University | Method for generating hypermutable organisms |
US6355412B1 (en) | 1999-07-09 | 2002-03-12 | The European Molecular Biology Laboratory | Methods and compositions for directed cloning and subcloning using homologous recombination |
US7026119B2 (en) * | 2000-02-11 | 2006-04-11 | Morphotek, Inc. | Methods for generating hypermutable microbes |
WO2001061012A1 (fr) | 2000-02-18 | 2001-08-23 | Nicolaides Nicholas C | Procede de generation de plantes tres sujettes aux mutations |
AU2001238558A1 (en) * | 2000-02-23 | 2001-09-03 | Luigi Grasso | Methods for generating hypermutable yeast |
US6569681B1 (en) | 2000-03-14 | 2003-05-27 | Transkaryotic Therapies, Inc. | Methods of improving homologous recombination |
US6596541B2 (en) * | 2000-10-31 | 2003-07-22 | Regeneron Pharmaceuticals, Inc. | Methods of modifying eukaryotic cells |
ATE501635T1 (de) | 2001-01-15 | 2011-04-15 | Morphotek Inc | Chemische hemmstoffe des mismatch-repair |
JP2005531291A (ja) | 2002-02-21 | 2005-10-20 | モーフオテク・インコーポレーテツド | Pmsr相同体を使用する超変異性細胞の作成方法 |
-
2003
- 2003-01-17 EP EP03703842A patent/EP1474522B1/fr not_active Expired - Lifetime
- 2003-01-17 AT AT03703842T patent/ATE548461T1/de active
- 2003-01-17 AU AU2003205173A patent/AU2003205173C1/en not_active Expired
- 2003-01-17 WO PCT/US2003/001361 patent/WO2003062435A1/fr not_active Application Discontinuation
- 2003-01-17 CA CA2473741A patent/CA2473741C/fr not_active Expired - Lifetime
- 2003-01-17 US US10/348,074 patent/US7638334B2/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
WO2003062435A1 (fr) | 2003-07-31 |
AU2003205173B2 (en) | 2008-05-22 |
CA2473741C (fr) | 2015-12-22 |
AU2003205173C1 (en) | 2009-01-29 |
EP1474522B1 (fr) | 2012-03-07 |
US20030176386A1 (en) | 2003-09-18 |
EP1474522A1 (fr) | 2004-11-10 |
EP1474522A4 (fr) | 2005-09-07 |
US7638334B2 (en) | 2009-12-29 |
ATE548461T1 (de) | 2012-03-15 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKEX | Expiry |
Effective date: 20230117 |