AU2001238558A1 - Methods for generating hypermutable yeast - Google Patents

Methods for generating hypermutable yeast

Info

Publication number
AU2001238558A1
AU2001238558A1 AU2001238558A AU3855801A AU2001238558A1 AU 2001238558 A1 AU2001238558 A1 AU 2001238558A1 AU 2001238558 A AU2001238558 A AU 2001238558A AU 3855801 A AU3855801 A AU 3855801A AU 2001238558 A1 AU2001238558 A1 AU 2001238558A1
Authority
AU
Australia
Prior art keywords
methods
repair system
mismatch repair
yeast
yeast cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001238558A
Inventor
Luigi Grasso
Kenneth W. Kinzler
Nicholas C. Nicolaides
Philip M. Sass
Bert Vogelstein
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Johns Hopkins University
Original Assignee
Johns Hopkins University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johns Hopkins University filed Critical Johns Hopkins University
Publication of AU2001238558A1 publication Critical patent/AU2001238558A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

Yeast cells are mutagenized to obtain desirable mutants. Mutagenesis is mediated by a defective mismatch repair system which can be enhanced using conventional exogenously applied mutagens. Yeast cells with the defective mismatch repair system are hypermutable, but after selection of desired mutant yeast strains, they can be rendered genetically stable by restoring the mismatch repair system to proper functionality.
AU2001238558A 2000-02-23 2001-02-21 Methods for generating hypermutable yeast Abandoned AU2001238558A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US18433600P 2000-02-23 2000-02-23
US60184336 2000-02-23
PCT/US2001/005447 WO2001062945A1 (en) 2000-02-23 2001-02-21 Methods for generating hypermutable yeast

Publications (1)

Publication Number Publication Date
AU2001238558A1 true AU2001238558A1 (en) 2001-09-03

Family

ID=22676473

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001238558A Abandoned AU2001238558A1 (en) 2000-02-23 2001-02-21 Methods for generating hypermutable yeast

Country Status (8)

Country Link
US (4) US6656736B2 (en)
EP (1) EP1259628B1 (en)
AT (1) ATE351914T1 (en)
AU (1) AU2001238558A1 (en)
CA (1) CA2399191C (en)
DE (1) DE60126065T2 (en)
DK (1) DK1259628T5 (en)
WO (1) WO2001062945A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2398094C (en) * 2000-02-11 2013-01-08 The Johns Hopkins University Methods for generating hypermutable microbes
DK1259628T5 (en) * 2000-02-23 2018-06-14 Univ Johns Hopkins Process for the formation of hypermutable yeast
US6825038B2 (en) 2000-05-12 2004-11-30 The Johns Hopkins University Method for generating hypermutable organisms
IL154099A0 (en) * 2000-07-27 2003-07-31 Univ Delaware A method and kit for enhancing oligonucleotide-mediated gene alteration
CA2473741C (en) * 2002-01-18 2015-12-22 Morphotek, Inc. A method for generating engineered cells for locus specific gene regulation and analysis
EP1572935A4 (en) * 2002-02-21 2006-09-13 Morphotek Inc Methods of making hypermutable cells using pmsr homologs
ATE428804T1 (en) * 2002-02-21 2009-05-15 Morphotek Inc REGULATED VECTORS FOR CONTROLLING DNA HYPERMUTABILITY IN EUKARYONTIC CELLS
WO2003104451A2 (en) 2002-06-06 2003-12-18 Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food Modifying dna recombination and repair
WO2008089355A1 (en) * 2007-01-17 2008-07-24 Life Technologies Corporation Methods and compositions for improving the health of cells in culture
WO2008103474A1 (en) 2007-02-20 2008-08-28 Anaptysbio, Inc. Methods of generating libraries and uses thereof
US20150307876A1 (en) * 2012-02-10 2015-10-29 Regents Of The University Of Minnesota Dna assimilation

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6191268B1 (en) 1993-12-17 2001-02-20 Dana-Farber Cancer Institute Compositions and methods relating to DNA mismatch repair genes
US5587290A (en) * 1995-06-26 1996-12-24 The Regents Of The University Of California Stress tolerant yeast mutants
US5843757A (en) 1995-08-24 1998-12-01 The Johns Hopkins University Human JTV1 gene overlaps PMS2 gene
US5907079A (en) 1996-01-18 1999-05-25 Amgen Canada Inc. MSH2 disrupted mice develop lymphomas
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
AUPO974597A0 (en) 1997-10-10 1997-11-06 Rhone-Poulenc Agro Methods for obtaining plant varieties
US6146894A (en) * 1998-04-14 2000-11-14 The Johns Hopkins University Method for generating hypermutable organisms
DK1259628T5 (en) * 2000-02-23 2018-06-14 Univ Johns Hopkins Process for the formation of hypermutable yeast

Also Published As

Publication number Publication date
CA2399191A1 (en) 2001-08-30
US20050272140A1 (en) 2005-12-08
US6656736B2 (en) 2003-12-02
CA2399191C (en) 2010-12-14
ATE351914T1 (en) 2007-02-15
US20040092021A1 (en) 2004-05-13
DK1259628T3 (en) 2007-05-21
DE60126065D1 (en) 2007-03-08
US20080176329A1 (en) 2008-07-24
US6921666B2 (en) 2005-07-26
EP1259628A1 (en) 2002-11-27
US7514216B2 (en) 2009-04-07
DE60126065T2 (en) 2007-08-30
WO2001062945A1 (en) 2001-08-30
EP1259628B1 (en) 2007-01-17
US7759121B2 (en) 2010-07-20
DK1259628T5 (en) 2018-06-14
US20020123149A1 (en) 2002-09-05

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