CA2468713A1 - Multiplex real-time quantitative pcr - Google Patents
Multiplex real-time quantitative pcr Download PDFInfo
- Publication number
- CA2468713A1 CA2468713A1 CA002468713A CA2468713A CA2468713A1 CA 2468713 A1 CA2468713 A1 CA 2468713A1 CA 002468713 A CA002468713 A CA 002468713A CA 2468713 A CA2468713 A CA 2468713A CA 2468713 A1 CA2468713 A1 CA 2468713A1
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- Prior art keywords
- pair
- pcr
- seq
- primer
- target nucleic
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
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| US33609501P | 2001-11-30 | 2001-11-30 | |
| US60/336,095 | 2001-11-30 | ||
| US39747502P | 2002-07-19 | 2002-07-19 | |
| US60/397,475 | 2002-07-19 | ||
| PCT/US2002/038806 WO2003048377A2 (en) | 2001-11-30 | 2002-12-02 | Multiplex real-time quantitative pcr |
Publications (1)
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|---|---|
| CA2468713A1 true CA2468713A1 (en) | 2003-06-12 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002468713A Abandoned CA2468713A1 (en) | 2001-11-30 | 2002-12-02 | Multiplex real-time quantitative pcr |
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| Country | Link |
|---|---|
| US (2) | US20050089862A1 (enExample) |
| EP (1) | EP1461350A4 (enExample) |
| JP (1) | JP2005511055A (enExample) |
| AU (2) | AU2002346660A1 (enExample) |
| CA (1) | CA2468713A1 (enExample) |
| WO (1) | WO2003048377A2 (enExample) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN100516211C (zh) * | 2002-02-20 | 2009-07-22 | 希森美康株式会社 | 用于持家基因mRNA检测的核酸扩增用引物和使用该引物的检查方法 |
| AU2003218925A1 (en) * | 2003-04-11 | 2004-11-01 | Dna Landmarks Inc. | Methods for relative quantification of specific nucleic acid sequences |
| GB2401175A (en) * | 2003-05-02 | 2004-11-03 | Hong Kong Dna Chips Ltd | Detection of SARS virus by PCR |
| WO2005042713A2 (en) | 2003-10-28 | 2005-05-12 | The Johns Hopkins University | Quantitative multiplex methylation-specific pcr |
| US20060057611A1 (en) * | 2004-06-30 | 2006-03-16 | Applera Corporation | Log-linear amplification |
| JP4898210B2 (ja) * | 2005-08-09 | 2012-03-14 | 株式会社ビー・エム・エル | サポウイルスの検出方法 |
| EP1777523A1 (en) | 2005-10-19 | 2007-04-25 | INSERM (Institut National de la Santé et de la Recherche Médicale) | An in vitro method for the prognosis of progression of a cancer and of the outcome in a patient and means for performing said method |
| DE102006027675B4 (de) | 2006-06-14 | 2011-05-12 | Siemens Ag | Verfahren zur Bestimmung der Konzentration von Nukleinsäuren |
| US20080050724A1 (en) * | 2006-08-24 | 2008-02-28 | Microfluidic Systems, Inc. | Method of detecting one or more limited copy targets |
| EP1961825A1 (en) | 2007-02-26 | 2008-08-27 | INSERM (Institut National de la Santé et de la Recherche Medicale) | Method for predicting the occurrence of metastasis in breast cancer patients |
| CA2688558A1 (en) * | 2007-06-04 | 2009-03-26 | Diagnoplex | Biomarker combinations for colorectal cancer |
| US20090253121A1 (en) * | 2008-04-04 | 2009-10-08 | Micah Halpern | Method for amt-rflp dna fingerprinting |
| US8346485B2 (en) | 2008-11-25 | 2013-01-01 | Quest Diagnostics Investments Incorporated | Methods and apparatuses for estimating initial target nucleic acid concentration in a sample by modeling background signal and cycle-dependent amplification efficiency of a polymerase chain reaction |
| FI20095544A0 (fi) | 2009-05-15 | 2009-05-15 | Juha Kirveskari | Menetelmä ja kitti antibioottiresistenttien bakteerien tunnistusta varten |
| CA2768391C (en) | 2009-07-21 | 2016-09-06 | Gen-Probe Incorporated | Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range |
| EP2721173A1 (en) | 2011-06-14 | 2014-04-23 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Methods for determining the expression level of a gene of interest including correction of rt-qpcr data for genomic dna-derived signals |
| EP2728015A1 (en) | 2012-10-31 | 2014-05-07 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Method for the Sezary's Syndrome diagnosis |
| WO2015150900A2 (en) | 2014-03-31 | 2015-10-08 | Debiopharm International Sa | Fgfr fusions |
| EP3126518B1 (en) | 2014-04-04 | 2018-12-12 | Life Technologies Corporation | Methods and systems for pcr quantitation |
| US10435747B2 (en) * | 2014-08-19 | 2019-10-08 | Arizona Board Of Regents On Behalf Of Arizona State University | Radiation biodosimetry systems |
| EP3020827A1 (en) * | 2014-11-13 | 2016-05-18 | Carpegen GmbH | Method for determining a property of a starting sample |
| JP7101397B2 (ja) * | 2016-12-02 | 2022-07-15 | 学校法人近畿大学 | 遺伝子量の測定方法 |
| JP6871598B2 (ja) * | 2017-01-18 | 2021-05-12 | 国立大学法人広島大学 | 遺伝子の変異検出キット及び遺伝子の変異検出方法 |
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| US6180349B1 (en) * | 1999-05-18 | 2001-01-30 | The Regents Of The University Of California | Quantitative PCR method to enumerate DNA copy number |
| US6605451B1 (en) * | 2000-06-06 | 2003-08-12 | Xtrana, Inc. | Methods and devices for multiplexing amplification reactions |
| US20040115671A1 (en) * | 2001-01-18 | 2004-06-17 | Zlokovic Berislav V | Gene expression profiling of endothelium in alzheimer's disease |
-
2002
- 2002-12-02 EP EP02784731A patent/EP1461350A4/en not_active Withdrawn
- 2002-12-02 CA CA002468713A patent/CA2468713A1/en not_active Abandoned
- 2002-12-02 WO PCT/US2002/038806 patent/WO2003048377A2/en not_active Ceased
- 2002-12-02 AU AU2002346660A patent/AU2002346660A1/en not_active Abandoned
- 2002-12-02 US US10/496,626 patent/US20050089862A1/en not_active Abandoned
- 2002-12-02 JP JP2003549554A patent/JP2005511055A/ja active Pending
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2008
- 2008-03-10 US US12/045,567 patent/US20090081655A1/en not_active Abandoned
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2009
- 2009-12-07 AU AU2009245826A patent/AU2009245826A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20050089862A1 (en) | 2005-04-28 |
| AU2002346660A1 (en) | 2003-06-17 |
| JP2005511055A (ja) | 2005-04-28 |
| AU2009245826A1 (en) | 2010-01-14 |
| US20090081655A1 (en) | 2009-03-26 |
| WO2003048377A2 (en) | 2003-06-12 |
| WO2003048377A3 (en) | 2004-05-06 |
| EP1461350A4 (en) | 2006-07-26 |
| EP1461350A2 (en) | 2004-09-29 |
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| EEER | Examination request | ||
| FZDE | Discontinued |