CA2438798A1 - Biosynthese de substrats de polycetide synthase - Google Patents

Biosynthese de substrats de polycetide synthase Download PDF

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Publication number
CA2438798A1
CA2438798A1 CA002438798A CA2438798A CA2438798A1 CA 2438798 A1 CA2438798 A1 CA 2438798A1 CA 002438798 A CA002438798 A CA 002438798A CA 2438798 A CA2438798 A CA 2438798A CA 2438798 A1 CA2438798 A1 CA 2438798A1
Authority
CA
Canada
Prior art keywords
polyketide
coa
hydroxybenzoate
production
didomain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002438798A
Other languages
English (en)
Inventor
Chaitan Khosla
Blaine Pfeifer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Leland Stanford Junior University
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/798,033 external-priority patent/US20020045220A1/en
Application filed by Individual filed Critical Individual
Publication of CA2438798A1 publication Critical patent/CA2438798A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1288—Transferases for other substituted phosphate groups (2.7.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/188—Heterocyclic compound containing in the condensed system at least one hetero ring having nitrogen atoms and oxygen atoms as the only ring heteroatoms
    • C12P17/189—Heterocyclic compound containing in the condensed system at least one hetero ring having nitrogen atoms and oxygen atoms as the only ring heteroatoms containing the rifamycin nucleus

Abstract

L'invention concerne l'utilisation d'enzymes qui catalysent la production d'unités d'amorçage et d'extension de polycétides. Elle concerne de plus des modules de chargement modifiés, pouvant recevoir diverses unités d'amorçage telles que des benzoates substitués, et qui peuvent servir à produire des dérivés substitués de produits naturels. Ces enzymes peuvent servir à améliorer le rendement de polycétides produits de façon native ou de polycétides mis au point rationnellement. A l'aide de ces techniques, la synthèse d'un polycétide complet a pu être mise en oeuvre chez <i>E. coli</i>. On peut améliorer la production de polycétides et d'autres métabolites secondaires chez des organismes microbiens en reportant celle-ci après une phase exponentielle et en maintenant des taux de nutriments relativement constants dans le milieu. De plus, on améliore la production de polycétides en prévoyant un système d'expression pour une thioestérase II. Une modification de l'organisme hôte et des conditions de culture permet ainsi d'améliorer la synthèse d'un métabolite secondaire. L'invention permet aussi d'obtenir un organisme hôte qui possède des caractéristiques souhaitables en vue de la production de polycétides et de l'évaluation des résultats de réarrangement génique.
CA002438798A 2001-02-28 2002-02-28 Biosynthese de substrats de polycetide synthase Abandoned CA2438798A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US09/798,033 2001-02-28
US09/798,033 US20020045220A1 (en) 1999-10-13 2001-02-28 Biosynthesis of polyketide synthase substrates
US35521102P 2002-02-08 2002-02-08
US60/355,211 2002-02-08
PCT/US2002/006399 WO2002068613A1 (fr) 2001-02-28 2002-02-28 Biosynthese de substrats de polycetide synthase

Publications (1)

Publication Number Publication Date
CA2438798A1 true CA2438798A1 (fr) 2002-09-06

Family

ID=26998747

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002438798A Abandoned CA2438798A1 (fr) 2001-02-28 2002-02-28 Biosynthese de substrats de polycetide synthase

Country Status (4)

Country Link
EP (1) EP1381672A4 (fr)
JP (1) JP2005506044A (fr)
CA (1) CA2438798A1 (fr)
WO (1) WO2002068613A1 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004007688A2 (fr) 2002-07-15 2004-01-22 Kosan Biosciences, Inc. Voies metaboliques pour des unites d'amorce dans la biosynthese polyketidique
CA2608579C (fr) * 2005-05-26 2019-09-10 Cytos Biotechnology Ag Procede de fermentation extensible
WO2012045088A2 (fr) * 2010-10-01 2012-04-05 The Board Of Trustees Of The University Of Illinois Conception combinatoire de trajets hétérologues très efficaces
US8852902B2 (en) 2010-11-22 2014-10-07 The Regents Of The University Of California Producing a trimethylpentanoic acid using hybrid polyketide synthases
US10233431B2 (en) 2014-02-26 2019-03-19 The Regents Of The University Of California Producing 3-hydroxycarboxylic acid and ketone using polyketide synthases
US11130784B2 (en) 2016-10-17 2021-09-28 Newsouth Innovations Pty Ltd Recombinant microcystin production
CN111944734B (zh) * 2019-05-17 2023-01-10 中国科学院微生物研究所 提高链霉菌聚酮化合物产量的方法
CN111394277B (zh) * 2020-03-05 2022-06-24 福建省微生物研究所 一种微生物转化制备雷帕霉素衍生物的菌株及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5672491A (en) * 1993-09-20 1997-09-30 The Leland Stanford Junior University Recombinant production of novel polyketides
US6033883A (en) * 1996-12-18 2000-03-07 Kosan Biosciences, Inc. Production of polyketides in bacteria and yeast
WO2000020601A2 (fr) * 1998-10-02 2000-04-13 Kosan Biosciences, Inc. Enzymes polyketides synthase et produits d'adn de recombinaisons associes
JP5142435B2 (ja) * 1999-10-13 2013-02-13 ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティ ポリケチド合成基質の生合成
WO2001031035A2 (fr) * 1999-10-27 2001-05-03 Kosan Biosciences, Inc. Production heterologue de polycetides

Also Published As

Publication number Publication date
JP2005506044A (ja) 2005-03-03
EP1381672A4 (fr) 2006-01-25
WO2002068613A1 (fr) 2002-09-06
EP1381672A1 (fr) 2004-01-21

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