CA2425100A1 - Procede pour creer des animaux clones par rearrangement chromosomique - Google Patents
Procede pour creer des animaux clones par rearrangement chromosomique Download PDFInfo
- Publication number
- CA2425100A1 CA2425100A1 CA002425100A CA2425100A CA2425100A1 CA 2425100 A1 CA2425100 A1 CA 2425100A1 CA 002425100 A CA002425100 A CA 002425100A CA 2425100 A CA2425100 A CA 2425100A CA 2425100 A1 CA2425100 A1 CA 2425100A1
- Authority
- CA
- Canada
- Prior art keywords
- isogenic
- animal
- cell
- haploid
- chromosome
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 241001465754 Metazoa Species 0.000 title claims abstract description 297
- 238000000034 method Methods 0.000 title claims abstract description 172
- 210000000349 chromosome Anatomy 0.000 title claims abstract description 63
- 238000012546 transfer Methods 0.000 claims abstract description 105
- 210000003765 sex chromosome Anatomy 0.000 claims abstract description 61
- 238000009395 breeding Methods 0.000 claims abstract description 41
- 230000001488 breeding effect Effects 0.000 claims abstract description 41
- 210000001766 X chromosome Anatomy 0.000 claims abstract description 32
- 210000002593 Y chromosome Anatomy 0.000 claims abstract description 21
- 230000014639 sexual reproduction Effects 0.000 claims abstract description 19
- 230000002759 chromosomal effect Effects 0.000 claims abstract description 15
- 241000124008 Mammalia Species 0.000 claims abstract description 11
- 208000031404 Chromosome Aberrations Diseases 0.000 claims abstract description 7
- 210000004027 cell Anatomy 0.000 claims description 117
- 210000003783 haploid cell Anatomy 0.000 claims description 116
- 210000001082 somatic cell Anatomy 0.000 claims description 94
- 210000004940 nucleus Anatomy 0.000 claims description 43
- 210000000287 oocyte Anatomy 0.000 claims description 42
- 210000002459 blastocyst Anatomy 0.000 claims description 36
- 210000000582 semen Anatomy 0.000 claims description 32
- 210000001161 mammalian embryo Anatomy 0.000 claims description 29
- 230000031864 metaphase Effects 0.000 claims description 28
- 210000003754 fetus Anatomy 0.000 claims description 19
- 230000021121 meiosis Effects 0.000 claims description 18
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 17
- 108090000623 proteins and genes Proteins 0.000 claims description 17
- 210000001840 diploid cell Anatomy 0.000 claims description 15
- 230000001939 inductive effect Effects 0.000 claims description 15
- 238000004519 manufacturing process Methods 0.000 claims description 14
- 239000008267 milk Substances 0.000 claims description 12
- 210000004080 milk Anatomy 0.000 claims description 12
- 235000013336 milk Nutrition 0.000 claims description 12
- 230000000392 somatic effect Effects 0.000 claims description 12
- 210000002308 embryonic cell Anatomy 0.000 claims description 11
- 239000003550 marker Substances 0.000 claims description 11
- 241000894007 species Species 0.000 claims description 11
- 241000283690 Bos taurus Species 0.000 claims description 10
- 230000004913 activation Effects 0.000 claims description 10
- 230000000735 allogeneic effect Effects 0.000 claims description 10
- 230000004927 fusion Effects 0.000 claims description 10
- 102000004169 proteins and genes Human genes 0.000 claims description 10
- 210000004508 polar body Anatomy 0.000 claims description 9
- 230000001568 sexual effect Effects 0.000 claims description 9
- 230000009027 insemination Effects 0.000 claims description 8
- 230000001404 mediated effect Effects 0.000 claims description 8
- 102000002322 Egg Proteins Human genes 0.000 claims description 7
- 108010000912 Egg Proteins Proteins 0.000 claims description 7
- 241000699666 Mus <mouse, genus> Species 0.000 claims description 7
- GBOGMAARMMDZGR-UHFFFAOYSA-N UNPD149280 Natural products N1C(=O)C23OC(=O)C=CC(O)CCCC(C)CC=CC3C(O)C(=C)C(C)C2C1CC1=CC=CC=C1 GBOGMAARMMDZGR-UHFFFAOYSA-N 0.000 claims description 7
- GBOGMAARMMDZGR-TYHYBEHESA-N cytochalasin B Chemical compound C([C@H]1[C@@H]2[C@@H](C([C@@H](O)[C@@H]3/C=C/C[C@H](C)CCC[C@@H](O)/C=C/C(=O)O[C@@]23C(=O)N1)=C)C)C1=CC=CC=C1 GBOGMAARMMDZGR-TYHYBEHESA-N 0.000 claims description 7
- GBOGMAARMMDZGR-JREHFAHYSA-N cytochalasin B Natural products C[C@H]1CCC[C@@H](O)C=CC(=O)O[C@@]23[C@H](C=CC1)[C@H](O)C(=C)[C@@H](C)[C@@H]2[C@H](Cc4ccccc4)NC3=O GBOGMAARMMDZGR-JREHFAHYSA-N 0.000 claims description 7
- 238000003780 insertion Methods 0.000 claims description 7
- 230000037431 insertion Effects 0.000 claims description 7
- 210000004681 ovum Anatomy 0.000 claims description 7
- 238000002689 xenotransplantation Methods 0.000 claims description 7
- 230000003213 activating effect Effects 0.000 claims description 6
- 230000008901 benefit Effects 0.000 claims description 6
- 230000022131 cell cycle Effects 0.000 claims description 6
- 108700028369 Alleles Proteins 0.000 claims description 5
- 206010008805 Chromosomal abnormalities Diseases 0.000 claims description 5
- 108010003272 Hyaluronate lyase Proteins 0.000 claims description 5
- 102000001974 Hyaluronidases Human genes 0.000 claims description 5
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 5
- 108700019146 Transgenes Proteins 0.000 claims description 5
- OPQGFIAVPSXOBO-UHFFFAOYSA-N bohemine Chemical compound N1=C(NCCCO)N=C2N(C(C)C)C=NC2=C1NCC1=CC=CC=C1 OPQGFIAVPSXOBO-UHFFFAOYSA-N 0.000 claims description 5
- 238000012239 gene modification Methods 0.000 claims description 5
- 230000005017 genetic modification Effects 0.000 claims description 5
- 235000013617 genetically modified food Nutrition 0.000 claims description 5
- 229960002773 hyaluronidase Drugs 0.000 claims description 5
- PGHMRUGBZOYCAA-UHFFFAOYSA-N ionomycin Natural products O1C(CC(O)C(C)C(O)C(C)C=CCC(C)CC(C)C(O)=CC(=O)C(C)CC(C)CC(CCC(O)=O)C)CCC1(C)C1OC(C)(C(C)O)CC1 PGHMRUGBZOYCAA-UHFFFAOYSA-N 0.000 claims description 5
- PGHMRUGBZOYCAA-ADZNBVRBSA-N ionomycin Chemical compound O1[C@H](C[C@H](O)[C@H](C)[C@H](O)[C@H](C)/C=C/C[C@@H](C)C[C@@H](C)C(/O)=C/C(=O)[C@@H](C)C[C@@H](C)C[C@@H](CCC(O)=O)C)CC[C@@]1(C)[C@@H]1O[C@](C)([C@@H](C)O)CC1 PGHMRUGBZOYCAA-ADZNBVRBSA-N 0.000 claims description 5
- -1 Ca2+ ions Chemical class 0.000 claims description 4
- 241000699670 Mus sp. Species 0.000 claims description 4
- 241000288906 Primates Species 0.000 claims description 4
- 206010052779 Transplant rejections Diseases 0.000 claims description 4
- 230000008859 change Effects 0.000 claims description 4
- 238000001125 extrusion Methods 0.000 claims description 4
- 238000002347 injection Methods 0.000 claims description 4
- 239000007924 injection Substances 0.000 claims description 4
- 108020004707 nucleic acids Proteins 0.000 claims description 4
- 102000039446 nucleic acids Human genes 0.000 claims description 4
- 150000007523 nucleic acids Chemical class 0.000 claims description 4
- 238000011282 treatment Methods 0.000 claims description 4
- 241000251468 Actinopterygii Species 0.000 claims description 3
- 241000283707 Capra Species 0.000 claims description 3
- 241000282326 Felis catus Species 0.000 claims description 3
- 241000283973 Oryctolagus cuniculus Species 0.000 claims description 3
- 241001494479 Pecora Species 0.000 claims description 3
- 241000282887 Suidae Species 0.000 claims description 3
- 210000002230 centromere Anatomy 0.000 claims description 3
- 238000012217 deletion Methods 0.000 claims description 3
- 230000037430 deletion Effects 0.000 claims description 3
- 239000000835 fiber Substances 0.000 claims description 3
- 210000004602 germ cell Anatomy 0.000 claims description 3
- 239000010985 leather Substances 0.000 claims description 3
- 150000002632 lipids Chemical class 0.000 claims description 3
- 235000013372 meat Nutrition 0.000 claims description 3
- 230000000394 mitotic effect Effects 0.000 claims description 3
- 239000003755 preservative agent Substances 0.000 claims description 3
- 230000002335 preservative effect Effects 0.000 claims description 3
- 230000000644 propagated effect Effects 0.000 claims description 3
- 230000005945 translocation Effects 0.000 claims description 3
- 210000002268 wool Anatomy 0.000 claims description 3
- 241000271566 Aves Species 0.000 claims description 2
- 241001416152 Bos frontalis Species 0.000 claims description 2
- 241000700198 Cavia Species 0.000 claims description 2
- 241000700199 Cavia porcellus Species 0.000 claims description 2
- 241000282693 Cercopithecidae Species 0.000 claims description 2
- 241000699800 Cricetinae Species 0.000 claims description 2
- 241000282412 Homo Species 0.000 claims description 2
- 241000700159 Rattus Species 0.000 claims description 2
- 235000015278 beef Nutrition 0.000 claims description 2
- 230000018486 cell cycle phase Effects 0.000 claims description 2
- 230000035558 fertility Effects 0.000 claims description 2
- 210000004962 mammalian cell Anatomy 0.000 claims description 2
- 235000016709 nutrition Nutrition 0.000 claims description 2
- 238000012360 testing method Methods 0.000 claims description 2
- 230000001225 therapeutic effect Effects 0.000 claims description 2
- 230000013011 mating Effects 0.000 claims 7
- 241000283705 Capra hircus Species 0.000 claims 2
- 108091000080 Phosphotransferase Proteins 0.000 claims 2
- 230000004077 genetic alteration Effects 0.000 claims 2
- 231100000118 genetic alteration Toxicity 0.000 claims 2
- 102000020233 phosphotransferase Human genes 0.000 claims 2
- 241000270322 Lepidosauria Species 0.000 claims 1
- 241001482592 Oreamnos americanus Species 0.000 claims 1
- 125000003158 alcohol group Chemical group 0.000 claims 1
- 238000010171 animal model Methods 0.000 claims 1
- 230000009261 transgenic effect Effects 0.000 abstract description 8
- 210000002257 embryonic structure Anatomy 0.000 description 12
- 238000010367 cloning Methods 0.000 description 10
- 238000005516 engineering process Methods 0.000 description 8
- 239000004615 ingredient Substances 0.000 description 8
- 238000011161 development Methods 0.000 description 7
- 230000018109 developmental process Effects 0.000 description 7
- 210000003917 human chromosome Anatomy 0.000 description 7
- 239000010902 straw Substances 0.000 description 7
- 108020004414 DNA Proteins 0.000 description 5
- 244000309464 bull Species 0.000 description 5
- 210000002950 fibroblast Anatomy 0.000 description 5
- 239000008188 pellet Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 4
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 4
- 230000002068 genetic effect Effects 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 241001550206 Colla Species 0.000 description 3
- 244000309466 calf Species 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 229910000162 sodium phosphate Inorganic materials 0.000 description 3
- 238000010186 staining Methods 0.000 description 3
- 239000006188 syrup Substances 0.000 description 3
- 235000020357 syrup Nutrition 0.000 description 3
- IAKHMKGGTNLKSZ-INIZCTEOSA-N (S)-colchicine Chemical compound C1([C@@H](NC(C)=O)CC2)=CC(=O)C(OC)=CC=C1C1=C2C=C(OC)C(OC)=C1OC IAKHMKGGTNLKSZ-INIZCTEOSA-N 0.000 description 2
- 241000282452 Ailuropoda melanoleuca Species 0.000 description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 2
- 102000011782 Keratins Human genes 0.000 description 2
- 108010076876 Keratins Proteins 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 102000006601 Thymidine Kinase Human genes 0.000 description 2
- 108020004440 Thymidine kinase Proteins 0.000 description 2
- 102000004243 Tubulin Human genes 0.000 description 2
- 108090000704 Tubulin Proteins 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000001110 calcium chloride Substances 0.000 description 2
- 235000011148 calcium chloride Nutrition 0.000 description 2
- 229910001628 calcium chloride Inorganic materials 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 235000013601 eggs Nutrition 0.000 description 2
- 230000001605 fetal effect Effects 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 238000010353 genetic engineering Methods 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 210000000723 mammalian artificial chromosome Anatomy 0.000 description 2
- 238000010449 nuclear transplantation Methods 0.000 description 2
- 229960003531 phenolsulfonphthalein Drugs 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 239000001103 potassium chloride Substances 0.000 description 2
- 235000011164 potassium chloride Nutrition 0.000 description 2
- 230000035935 pregnancy Effects 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 230000002062 proliferating effect Effects 0.000 description 2
- RXWNCPJZOCPEPQ-NVWDDTSBSA-N puromycin Chemical compound C1=CC(OC)=CC=C1C[C@H](N)C(=O)N[C@H]1[C@@H](O)[C@H](N2C3=NC=NC(=C3N=C2)N(C)C)O[C@@H]1CO RXWNCPJZOCPEPQ-NVWDDTSBSA-N 0.000 description 2
- 238000005204 segregation Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000011550 stock solution Substances 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- CYDQOEWLBCCFJZ-UHFFFAOYSA-N 4-(4-fluorophenyl)oxane-4-carboxylic acid Chemical compound C=1C=C(F)C=CC=1C1(C(=O)O)CCOCC1 CYDQOEWLBCCFJZ-UHFFFAOYSA-N 0.000 description 1
- 206010000234 Abortion spontaneous Diseases 0.000 description 1
- 241001455214 Acinonyx jubatus Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 241000282985 Cervus Species 0.000 description 1
- 241000938605 Crocodylia Species 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- 241000283086 Equidae Species 0.000 description 1
- 230000010337 G2 phase Effects 0.000 description 1
- 102100028701 General vesicular transport factor p115 Human genes 0.000 description 1
- 101150104214 HGPRT gene Proteins 0.000 description 1
- 101000767151 Homo sapiens General vesicular transport factor p115 Proteins 0.000 description 1
- 102000018251 Hypoxanthine Phosphoribosyltransferase Human genes 0.000 description 1
- 108010091358 Hypoxanthine Phosphoribosyltransferase Proteins 0.000 description 1
- 108010025815 Kanamycin Kinase Proteins 0.000 description 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 206010027476 Metastases Diseases 0.000 description 1
- 229930193140 Neomycin Natural products 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241000283903 Ovis aries Species 0.000 description 1
- 241000282376 Panthera tigris Species 0.000 description 1
- 206010060862 Prostate cancer Diseases 0.000 description 1
- 208000000236 Prostatic Neoplasms Diseases 0.000 description 1
- 241000982634 Tragelaphus eurycerus Species 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 102000006646 aminoglycoside phosphotransferase Human genes 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 238000012832 cell culture technique Methods 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 229960001338 colchicine Drugs 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 210000001771 cumulus cell Anatomy 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 230000003511 endothelial effect Effects 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000003328 fibroblastic effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- IRSCQMHQWWYFCW-UHFFFAOYSA-N ganciclovir Chemical compound O=C1NC(N)=NC2=C1N=CN2COC(CO)CO IRSCQMHQWWYFCW-UHFFFAOYSA-N 0.000 description 1
- 229960002963 ganciclovir Drugs 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 210000002503 granulosa cell Anatomy 0.000 description 1
- 230000006801 homologous recombination Effects 0.000 description 1
- 238000002744 homologous recombination Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000009399 inbreeding Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 229940001447 lactate Drugs 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 238000002826 magnetic-activated cell sorting Methods 0.000 description 1
- 210000000415 mammalian chromosome Anatomy 0.000 description 1
- 210000005075 mammary gland Anatomy 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012092 media component Substances 0.000 description 1
- 230000009401 metastasis Effects 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 238000002406 microsurgery Methods 0.000 description 1
- 230000011278 mitosis Effects 0.000 description 1
- 229960004927 neomycin Drugs 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 210000003899 penis Anatomy 0.000 description 1
- RLZZZVKAURTHCP-UHFFFAOYSA-N phenanthrene-3,4-diol Chemical compound C1=CC=C2C3=C(O)C(O)=CC=C3C=CC2=C1 RLZZZVKAURTHCP-UHFFFAOYSA-N 0.000 description 1
- 210000002826 placenta Anatomy 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 229950010131 puromycin Drugs 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 238000007634 remodeling Methods 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 230000008672 reprogramming Effects 0.000 description 1
- 210000002027 skeletal muscle Anatomy 0.000 description 1
- 238000007390 skin biopsy Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000001540 sodium lactate Substances 0.000 description 1
- 235000011088 sodium lactate Nutrition 0.000 description 1
- 229940005581 sodium lactate Drugs 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 208000000995 spontaneous abortion Diseases 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 210000001550 testis Anatomy 0.000 description 1
- 229940126585 therapeutic drug Drugs 0.000 description 1
- 208000019179 thyroid gland undifferentiated (anaplastic) carcinoma Diseases 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 231100000588 tumorigenic Toxicity 0.000 description 1
- 230000000381 tumorigenic effect Effects 0.000 description 1
- 210000001215 vagina Anatomy 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
- C12N15/877—Techniques for producing new mammalian cloned embryos
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0273—Cloned vertebrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/101—Bovine
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/30—Bird
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/40—Fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/02—Animal zootechnically ameliorated
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/02—Animal zootechnically ameliorated
- A01K2267/025—Animal producing cells or organs for transplantation
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Developmental Biology & Embryology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Veterinary Medicine (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US23801400P | 2000-10-06 | 2000-10-06 | |
US60/238,014 | 2000-10-06 | ||
PCT/US2001/031216 WO2002029002A2 (fr) | 2000-10-06 | 2001-10-05 | Procede pour creer des animaux clones par rearrangement chromosomique |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2425100A1 true CA2425100A1 (fr) | 2002-04-11 |
Family
ID=22896126
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002425100A Abandoned CA2425100A1 (fr) | 2000-10-06 | 2001-10-05 | Procede pour creer des animaux clones par rearrangement chromosomique |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP1334356A4 (fr) |
AU (1) | AU2002213033A1 (fr) |
CA (1) | CA2425100A1 (fr) |
WO (1) | WO2002029002A2 (fr) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE10248361A1 (de) * | 2002-06-30 | 2004-01-22 | Beisswanger, Roland, Dr. | Unterdrückung eines Geschlechtes durch die Veränderung von Geschlechtschromosomen |
CN114457360B (zh) * | 2022-02-11 | 2023-08-25 | 中国华能集团清洁能源技术研究院有限公司 | 一种无隔膜微电解槽放大设备及加工方法和应用 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5270201A (en) * | 1988-03-24 | 1993-12-14 | The General Hospital Corporation | Artificial chromosome vector |
US5240840A (en) * | 1991-04-05 | 1993-08-31 | Regents Of The University Of Michigan | Dna superfragment cloning |
US6077697A (en) * | 1996-04-10 | 2000-06-20 | Chromos Molecular Systems, Inc. | Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes |
US6331659B1 (en) * | 1998-01-21 | 2001-12-18 | University Of Hawaii | Cumulus cells as nuclear donors |
EP1141265B1 (fr) * | 1999-01-13 | 2008-09-03 | Revivicor, Inc. | Procede de double transfert de noyau et resultats de ce transfert |
AU2002250402A1 (en) * | 2001-03-23 | 2002-10-08 | Advanced Cell Technology, Inc. | Method for generating cloned animals using chromosome shuffling |
-
2001
- 2001-10-05 WO PCT/US2001/031216 patent/WO2002029002A2/fr active Application Filing
- 2001-10-05 AU AU2002213033A patent/AU2002213033A1/en not_active Abandoned
- 2001-10-05 CA CA002425100A patent/CA2425100A1/fr not_active Abandoned
- 2001-10-05 EP EP01981389A patent/EP1334356A4/fr not_active Withdrawn
Also Published As
Publication number | Publication date |
---|---|
EP1334356A2 (fr) | 2003-08-13 |
WO2002029002A2 (fr) | 2002-04-11 |
AU2002213033A1 (en) | 2002-04-15 |
EP1334356A4 (fr) | 2004-10-06 |
WO2002029002A3 (fr) | 2002-09-19 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Li et al. | Cloned ferrets produced by somatic cell nuclear transfer | |
Krimpenfort et al. | Generation of transgenic dairy cattle using ‘in vitro’embryo production | |
US20020174449A1 (en) | Method for generating cloned animals using chromosome shuffling | |
Betthauser et al. | Production of cloned pigs from in vitro systems | |
US5523226A (en) | Transgenic swine compositions and methods | |
Heyman | Nuclear transfer: a new tool for reproductive biotechnology in cattle | |
JP4102450B2 (ja) | 胎仔線維芽細胞による効率的な核移入 | |
JP2003512848A (ja) | 遺伝的診断、修飾および増殖のための一倍体ゲノムの使用 | |
WO1998027214A1 (fr) | Production d'ongules chimeriques et transgeniques a l'aide de cellules souches embryonnaires | |
EA017100B1 (ru) | Способ переноса клеточных ядер, способ получения генетически модифицированного или трансгенного млекопитающего, способ клонирования млекопитающего, генетически модифицированное млекопитающее и генетически модифицированный эмбрион | |
KR20180128386A (ko) | 유전적 상보성에 의한 인간화 신장의 조작 | |
Skrzyszowska et al. | Generation of transgenic rabbits by the novel technique of chimeric somatic cell cloning | |
US20040040050A1 (en) | Production of agricultural animals from embryonic stem (es) cells | |
US20040139489A1 (en) | Method for generating cloned animals using chromosome shuffling | |
WO2020047514A2 (fr) | Procédé de production de modèles animaux avec des phénotypes associés à une maladie | |
US6498285B1 (en) | Methods for producing transgenic pigs by microinjecting a blastomere | |
CA2396210A1 (fr) | Procede de clonage d'animaux avec modifications genetiques ciblees, par transfert de noyaux cellulaires somatiques males ou femelles a culture a long terme, comprenant des modifications genetiques provoquees artificiellement, dans des cellules receveuses enuclees | |
CA2425100A1 (fr) | Procede pour creer des animaux clones par rearrangement chromosomique | |
Basrur et al. | Genetics then and now: breeding the best and biotechnology | |
JP4845073B2 (ja) | 再構築受精卵の作製方法及びそれを用いたトランスジェニック胚の作製方法 | |
US20040031069A1 (en) | Cloning endangered and extinct species | |
WO2021193596A1 (fr) | Milieu de traitement temporaire, kit de traitement, inhibiteur d'arrêt de développement embryonnaire, procédé d'inhibition d'arrêt de développement embryonnaire, procédé de production de produit d'ingénierie de développement, procédé de transfert, méthode thérapeutique et produit d'ingénierie de développement | |
JP4903392B2 (ja) | 遺伝子ホモ改変哺乳動物細胞、遺伝子ホモ改変非ヒト哺乳動物、及びそれらの樹立、作出方法。 | |
US20060174358A1 (en) | Method for cloning animals with targetted genetic alterations by transfer of long-term cultured male or female somatic cell nuclei, comprising artificially-induced genetic alterations, to enucleated recipient cells | |
Gou et al. | Transgenic twin lambs cloned by granulosa cells |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FZDE | Discontinued |