CA2414396A1 - Production de vaccins par utilisation de plantes transgeniques ou de virus de plantes modifies comme vecteurs d'expression et proteines de coque virales transencapsidees utiliseescomme systemes de presentation d'epitopes - Google Patents

Production de vaccins par utilisation de plantes transgeniques ou de virus de plantes modifies comme vecteurs d'expression et proteines de coque virales transencapsidees utiliseescomme systemes de presentation d'epitopes Download PDF

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Publication number
CA2414396A1
CA2414396A1 CA002414396A CA2414396A CA2414396A1 CA 2414396 A1 CA2414396 A1 CA 2414396A1 CA 002414396 A CA002414396 A CA 002414396A CA 2414396 A CA2414396 A CA 2414396A CA 2414396 A1 CA2414396 A1 CA 2414396A1
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CA
Canada
Prior art keywords
plant
coat protein
bymv
seq
epitope
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002414396A
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English (en)
Inventor
Rosemarie Hammond
Yan Zhao
John Hammond
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
US Department of Agriculture USDA
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2414396A1 publication Critical patent/CA2414396A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16111—Human Immunodeficiency Virus, HIV concerning HIV env
    • C12N2740/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/18011—Paramyxoviridae
    • C12N2760/18111—Avulavirus, e.g. Newcastle disease virus
    • C12N2760/18122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/34011—Potyviridae
    • C12N2770/34022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Virology (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Selon l'invention, des plantes infectées pas le virus Y de la pomme de terre (PVY) ont été inoculées avec de l'ARN de PVX infectieux codant pour la protéine de coque (PC) de PVX et pour la protéine PC chimérique de NDV/BYMV. L'ELISA indirect d'une plaque recouverte d'antigène et l'immunoélectromicroscopie d'un virus purifié à partir de plantes infectées ont montré que les virions descendants présentaient une concentration de protéine PC chimérique comprise entre moins de 1 % et 25 %. Une méthode peut être utilisée pour produire et purifier le vaccin contre NDV en grande quantité. Des virions de PVY transencapsidés exprimant NDV ont été purifiés et utilisés pour induire des anticorps dirigés contre NDV dans des souris. Par ailleurs, des plantes de tabac transgéniques exprimant la protéine PC de VIH/BYMV ont été inoculées avec BYMV. Les virions descendants issus de plantes transgéniques contenaient la protéine PC transgénique de VIH/BYMV.
CA002414396A 2000-06-26 2001-06-26 Production de vaccins par utilisation de plantes transgeniques ou de virus de plantes modifies comme vecteurs d'expression et proteines de coque virales transencapsidees utiliseescomme systemes de presentation d'epitopes Abandoned CA2414396A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US60399700A 2000-06-26 2000-06-26
US09/603,997 2000-06-26
PCT/US2001/020272 WO2002000169A2 (fr) 2000-06-26 2001-06-26 Production de vaccins par utilisation de plantes transgeniques ou de virus de plantes modifies comme vecteurs d'expression et proteines de coque virales transencapsidees utilisees comme systemes de presentation d'epitopes

Publications (1)

Publication Number Publication Date
CA2414396A1 true CA2414396A1 (fr) 2002-01-03

Family

ID=24417757

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002414396A Abandoned CA2414396A1 (fr) 2000-06-26 2001-06-26 Production de vaccins par utilisation de plantes transgeniques ou de virus de plantes modifies comme vecteurs d'expression et proteines de coque virales transencapsidees utiliseescomme systemes de presentation d'epitopes

Country Status (3)

Country Link
AU (1) AU2001270164A1 (fr)
CA (1) CA2414396A1 (fr)
WO (1) WO2002000169A2 (fr)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001270164A1 (en) * 2000-06-26 2002-01-08 The United States Of America As Represented By The Secretary Of Agriculture Production of vaccines using transgenic plants
DK1523329T3 (da) 2002-07-05 2013-10-14 Folia Biotech Inc Viruspartikeladjuvant
US8101189B2 (en) 2002-07-05 2012-01-24 Folia Biotech Inc. Vaccines and immunopotentiating compositions and methods for making and using them
NZ543348A (en) * 2003-05-05 2008-08-29 Thompson Boyce Plant Res Vectors and cells for preparing immunoprotective compositions derived from transgenic plants
WO2005108564A2 (fr) * 2004-03-25 2005-11-17 Large Scale Biology Corporation Production de peptides dans des plantes sous forme de fusion de protéines d'enveloppe virale
EP2290071B1 (fr) 2004-05-28 2014-12-31 Asuragen, Inc. Procédés et compositions impliquant du microARN
US20050287539A1 (en) * 2004-06-29 2005-12-29 Emmanuel Labourier Methods and compositions for preparing capped RNA
DK2302055T3 (da) 2004-11-12 2014-10-13 Asuragen Inc Fremgangsmåder og sammensætninger involverende miRNA og miRNA-inhibitormolekyler
US20100047264A1 (en) 2006-11-15 2010-02-25 Folia Biotech Inc. Immunogenic Affinity-Conjugated Antigen Systems Based on Papaya Mosaic Virus and Uses Thereof
WO2009036332A1 (fr) 2007-09-14 2009-03-19 Asuragen, Inc. Microarn exprimés de manière différentielle dans le cancer du col de l'utérus et leurs utilisations
RU2370280C2 (ru) * 2007-10-08 2009-10-20 Институт физико-химической биологии им. А.Н.Белозерского СПОСОБ СУПЕРПРОДУКЦИИ В РАСТЕНИИ АНТИТЕЛ ПРОТИВ ОНКОГЕНА HER2/neu
WO2009137807A2 (fr) 2008-05-08 2009-11-12 Asuragen, Inc. Compositions et procédés liés à la modulation de miarn de néovascularisation ou d’angiogenèse
EP2327764B1 (fr) 2009-11-30 2011-12-28 United Cancer Research Institute Nouveau clone du virus de la maladie de Newcastle, sa fabrication et son application dans le traitement médical du cancer
WO2013040251A2 (fr) 2011-09-13 2013-03-21 Asurgen, Inc. Méthodes et compositions incluant mir-135b, permettant de faire la distinction entre un cancer du pancréas et une maladie pancréatique bénigne
CN108315493A (zh) * 2018-04-23 2018-07-24 上海市农业科学院 一种用于检测大麦黄花叶病毒的lamp引物组及检测方法
CN109486992B (zh) * 2018-12-11 2021-08-20 贵州省烟草科学研究院 烟草eIF4E-1位点大片段缺失突变的特异性共显性分子标记及应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5041385A (en) * 1986-11-01 1991-08-20 Oxford Gene Systems Limited Vector expressing fusion proteins and particles
AU639891B2 (en) * 1988-08-19 1993-08-12 Seminis Vegetable Seeds, Inc. Expression cassette for plants
US4970168A (en) * 1989-01-27 1990-11-13 Monsanto Company Virus-resistant plants
WO1991015587A1 (fr) * 1990-04-06 1991-10-17 Commonwealth Scientific And Industrial Research Organisation Systeme d'expression auto-polymerisante a base de proteines enveloppantes de potyvirus modifiees
GB9108386D0 (en) * 1991-04-19 1991-06-05 Agricultural Genetics Co Modified plant viruses as vectors
US5955342A (en) * 1994-08-15 1999-09-21 Connaught Laboratories Limited Non-infectious, replication-defective, self-assembling HIV-1 viral particles containing antigenic markers in the gag coding region
CA2202761A1 (fr) * 1994-10-18 1996-04-25 Sean Nicholas Chapman Procede de production de proteines chimeres
GB9712282D0 (en) * 1997-06-12 1997-08-13 Innes John Centre Innov Ltd Epitope presentation system
AU2001270164A1 (en) * 2000-06-26 2002-01-08 The United States Of America As Represented By The Secretary Of Agriculture Production of vaccines using transgenic plants

Also Published As

Publication number Publication date
AU2001270164A1 (en) 2002-01-08
WO2002000169A2 (fr) 2002-01-03
WO2002000169A3 (fr) 2002-07-18

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FZDE Discontinued