CA2414396A1 - Production de vaccins par utilisation de plantes transgeniques ou de virus de plantes modifies comme vecteurs d'expression et proteines de coque virales transencapsidees utiliseescomme systemes de presentation d'epitopes - Google Patents
Production de vaccins par utilisation de plantes transgeniques ou de virus de plantes modifies comme vecteurs d'expression et proteines de coque virales transencapsidees utiliseescomme systemes de presentation d'epitopes Download PDFInfo
- Publication number
- CA2414396A1 CA2414396A1 CA002414396A CA2414396A CA2414396A1 CA 2414396 A1 CA2414396 A1 CA 2414396A1 CA 002414396 A CA002414396 A CA 002414396A CA 2414396 A CA2414396 A CA 2414396A CA 2414396 A1 CA2414396 A1 CA 2414396A1
- Authority
- CA
- Canada
- Prior art keywords
- plant
- coat protein
- bymv
- seq
- epitope
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
- C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16111—Human Immunodeficiency Virus, HIV concerning HIV env
- C12N2740/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/18011—Paramyxoviridae
- C12N2760/18111—Avulavirus, e.g. Newcastle disease virus
- C12N2760/18122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/34011—Potyviridae
- C12N2770/34022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Pharmacology & Pharmacy (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Virology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Selon l'invention, des plantes infectées pas le virus Y de la pomme de terre (PVY) ont été inoculées avec de l'ARN de PVX infectieux codant pour la protéine de coque (PC) de PVX et pour la protéine PC chimérique de NDV/BYMV. L'ELISA indirect d'une plaque recouverte d'antigène et l'immunoélectromicroscopie d'un virus purifié à partir de plantes infectées ont montré que les virions descendants présentaient une concentration de protéine PC chimérique comprise entre moins de 1 % et 25 %. Une méthode peut être utilisée pour produire et purifier le vaccin contre NDV en grande quantité. Des virions de PVY transencapsidés exprimant NDV ont été purifiés et utilisés pour induire des anticorps dirigés contre NDV dans des souris. Par ailleurs, des plantes de tabac transgéniques exprimant la protéine PC de VIH/BYMV ont été inoculées avec BYMV. Les virions descendants issus de plantes transgéniques contenaient la protéine PC transgénique de VIH/BYMV.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US60399700A | 2000-06-26 | 2000-06-26 | |
US09/603,997 | 2000-06-26 | ||
PCT/US2001/020272 WO2002000169A2 (fr) | 2000-06-26 | 2001-06-26 | Production de vaccins par utilisation de plantes transgeniques ou de virus de plantes modifies comme vecteurs d'expression et proteines de coque virales transencapsidees utilisees comme systemes de presentation d'epitopes |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2414396A1 true CA2414396A1 (fr) | 2002-01-03 |
Family
ID=24417757
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002414396A Abandoned CA2414396A1 (fr) | 2000-06-26 | 2001-06-26 | Production de vaccins par utilisation de plantes transgeniques ou de virus de plantes modifies comme vecteurs d'expression et proteines de coque virales transencapsidees utiliseescomme systemes de presentation d'epitopes |
Country Status (3)
Country | Link |
---|---|
AU (1) | AU2001270164A1 (fr) |
CA (1) | CA2414396A1 (fr) |
WO (1) | WO2002000169A2 (fr) |
Families Citing this family (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2414396A1 (fr) * | 2000-06-26 | 2002-01-03 | The United States Of America, As Represented By The Secretary Of Agricul Ture | Production de vaccins par utilisation de plantes transgeniques ou de virus de plantes modifies comme vecteurs d'expression et proteines de coque virales transencapsidees utiliseescomme systemes de presentation d'epitopes |
US8101189B2 (en) | 2002-07-05 | 2012-01-24 | Folia Biotech Inc. | Vaccines and immunopotentiating compositions and methods for making and using them |
CN1665528B (zh) | 2002-07-05 | 2013-05-15 | 弗利亚生物技术公司 | 佐剂病毒颗粒 |
BRPI0410340A (pt) * | 2003-05-05 | 2006-05-30 | Dow Agrosciences Llc | vetores e células para preparar composições imunoprotetoras derivadas de plantas transgênicas |
WO2005108564A2 (fr) * | 2004-03-25 | 2005-11-17 | Large Scale Biology Corporation | Production de peptides dans des plantes sous forme de fusion de protéines d'enveloppe virale |
EP2471924A1 (fr) | 2004-05-28 | 2012-07-04 | Asuragen, INC. | Procédés et compositions impliquant du microARN |
US20050287539A1 (en) * | 2004-06-29 | 2005-12-29 | Emmanuel Labourier | Methods and compositions for preparing capped RNA |
CA2850323A1 (fr) | 2004-11-12 | 2006-12-28 | Asuragen, Inc. | Procedes et compositions comprenant des molecules de micro-arn et des molecules d'inhibiteur de micro-arn |
US20100047264A1 (en) | 2006-11-15 | 2010-02-25 | Folia Biotech Inc. | Immunogenic Affinity-Conjugated Antigen Systems Based on Papaya Mosaic Virus and Uses Thereof |
WO2009036332A1 (fr) | 2007-09-14 | 2009-03-19 | Asuragen, Inc. | Microarn exprimés de manière différentielle dans le cancer du col de l'utérus et leurs utilisations |
RU2370280C2 (ru) * | 2007-10-08 | 2009-10-20 | Институт физико-химической биологии им. А.Н.Белозерского | СПОСОБ СУПЕРПРОДУКЦИИ В РАСТЕНИИ АНТИТЕЛ ПРОТИВ ОНКОГЕНА HER2/neu |
US8258111B2 (en) | 2008-05-08 | 2012-09-04 | The Johns Hopkins University | Compositions and methods related to miRNA modulation of neovascularization or angiogenesis |
ES2380289T3 (es) | 2009-11-30 | 2012-05-10 | United Cancer Research Institute | Nuevo clon del virus de la enfermedad de Newcastle, su fabricación y aplicación en el tratamiento médico del cáncer |
US9644241B2 (en) | 2011-09-13 | 2017-05-09 | Interpace Diagnostics, Llc | Methods and compositions involving miR-135B for distinguishing pancreatic cancer from benign pancreatic disease |
CN108315493A (zh) * | 2018-04-23 | 2018-07-24 | 上海市农业科学院 | 一种用于检测大麦黄花叶病毒的lamp引物组及检测方法 |
CN109486992B (zh) * | 2018-12-11 | 2021-08-20 | 贵州省烟草科学研究院 | 烟草eIF4E-1位点大片段缺失突变的特异性共显性分子标记及应用 |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5041385A (en) * | 1986-11-01 | 1991-08-20 | Oxford Gene Systems Limited | Vector expressing fusion proteins and particles |
WO1990002184A1 (fr) * | 1988-08-19 | 1990-03-08 | The Upjohn Company | Genes de la proteine d'enveloppe de potyvirus et plantes transformees au moyen de tels genes |
US4970168A (en) * | 1989-01-27 | 1990-11-13 | Monsanto Company | Virus-resistant plants |
WO1991015587A1 (fr) * | 1990-04-06 | 1991-10-17 | Commonwealth Scientific And Industrial Research Organisation | Systeme d'expression auto-polymerisante a base de proteines enveloppantes de potyvirus modifiees |
GB9108386D0 (en) * | 1991-04-19 | 1991-06-05 | Agricultural Genetics Co | Modified plant viruses as vectors |
US5955342A (en) * | 1994-08-15 | 1999-09-21 | Connaught Laboratories Limited | Non-infectious, replication-defective, self-assembling HIV-1 viral particles containing antigenic markers in the gag coding region |
EP0787193A1 (fr) * | 1994-10-18 | 1997-08-06 | Scottish Crop Research Institute | Procede de production de proteines chimeres |
GB9712282D0 (en) * | 1997-06-12 | 1997-08-13 | Innes John Centre Innov Ltd | Epitope presentation system |
CA2414396A1 (fr) * | 2000-06-26 | 2002-01-03 | The United States Of America, As Represented By The Secretary Of Agricul Ture | Production de vaccins par utilisation de plantes transgeniques ou de virus de plantes modifies comme vecteurs d'expression et proteines de coque virales transencapsidees utiliseescomme systemes de presentation d'epitopes |
-
2001
- 2001-06-26 CA CA002414396A patent/CA2414396A1/fr not_active Abandoned
- 2001-06-26 AU AU2001270164A patent/AU2001270164A1/en not_active Abandoned
- 2001-06-26 WO PCT/US2001/020272 patent/WO2002000169A2/fr active Application Filing
Also Published As
Publication number | Publication date |
---|---|
WO2002000169A2 (fr) | 2002-01-03 |
WO2002000169A3 (fr) | 2002-07-18 |
AU2001270164A1 (en) | 2002-01-08 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FZDE | Discontinued |