CA2403190A1 - Procede de separation de proteines par electrophorese capillaire et compositions de tampon pour electrophorese capillaire - Google Patents

Procede de separation de proteines par electrophorese capillaire et compositions de tampon pour electrophorese capillaire Download PDF

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Publication number
CA2403190A1
CA2403190A1 CA002403190A CA2403190A CA2403190A1 CA 2403190 A1 CA2403190 A1 CA 2403190A1 CA 002403190 A CA002403190 A CA 002403190A CA 2403190 A CA2403190 A CA 2403190A CA 2403190 A1 CA2403190 A1 CA 2403190A1
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Canada
Prior art keywords
capillary electrophoresis
buffer compositions
separating proteins
analysis buffer
constituents
Prior art date
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CA002403190A
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English (en)
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CA2403190C (fr
Inventor
Georges Nouadje
Frederic Robert
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Sebia SA
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Publication of CA2403190A1 publication Critical patent/CA2403190A1/fr
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Publication of CA2403190C publication Critical patent/CA2403190C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Dispersion Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)

Abstract

L'invention concerne un procédé d'électrophorèse capillaire en solution libre à pH alcalin pour l'analyse d'échan-tillons biologiques comportant des constituants protéiques, caractérisé en ce qu'il comporte au moins une étape où on introduit l'échantillon dans un tube capillaire contenant un tampon d'analyse, ledit tampon d'analyse comportant en outre au moins additif capable d'intercation hydrophobe avec un ou plusieurs constituants protéiques et capable d'apporter à ce ou ces constituants pro-téiques une ou plusieurs charges négatives et d'en modifier la mobilité
électrophorétique. Elle concerne également des compositions de tampon d'analyse pour sa mise en oeuvre.
CA002403190A 2001-01-19 2002-01-18 Procede de separation de proteines par electrophorese capillaire et compositions de tampon pour electrophorese capillaire Expired - Lifetime CA2403190C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR01/00762 2001-01-19
FR0100762A FR2819888B1 (fr) 2001-01-19 2001-01-19 Procede de separation de proteines par electrophorese capillaire et compositions de tampon pour electrophorese capillaire
PCT/FR2002/000216 WO2002057737A2 (fr) 2001-01-19 2002-01-18 Procede de separation de proteines par electrophorese capillaire et compositions de tampon pour electrophorese capillaire

Publications (2)

Publication Number Publication Date
CA2403190A1 true CA2403190A1 (fr) 2002-07-25
CA2403190C CA2403190C (fr) 2008-05-06

Family

ID=8859042

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002403190A Expired - Lifetime CA2403190C (fr) 2001-01-19 2002-01-18 Procede de separation de proteines par electrophorese capillaire et compositions de tampon pour electrophorese capillaire

Country Status (13)

Country Link
US (1) US7297244B2 (fr)
EP (1) EP1229325B1 (fr)
JP (1) JP4216598B2 (fr)
CN (2) CN1630816A (fr)
BR (1) BR0203699B1 (fr)
CA (1) CA2403190C (fr)
DK (1) DK1229325T3 (fr)
ES (1) ES2557740T3 (fr)
FR (1) FR2819888B1 (fr)
HK (1) HK1143862A1 (fr)
NZ (1) NZ549342A (fr)
PT (1) PT1229325E (fr)
WO (1) WO2002057737A2 (fr)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2819889B1 (fr) * 2001-01-19 2003-08-01 Sebia Sa Procede d'electrophorese capillaire en solution libre a ph alcalin
FR2862134B1 (fr) 2003-11-12 2007-07-27 Sebia Sa Analyse et typage de proteines monoclonales par electrophorese capillaire et immunodeplacement
FR2869995B1 (fr) * 2004-05-10 2006-09-22 Sebia Sa Procede ameliore de separation de proteines par electrophorese capillaire et compositions de tampon pour electrophorese capillaire
FR2878033B1 (fr) * 2004-11-18 2007-05-04 Sebia Sa Procede d'analyse de l'hemoglobine par electrophorese capillaire, trousse pour electrophorese capillaire et utilisation de ralentisseur de flux dans un tel procede
US20060160235A1 (en) * 2004-11-19 2006-07-20 Craighead Harold G Separation of denatured proteins in free solution
EP1845122B1 (fr) * 2005-02-04 2011-11-16 JSR Corporation Polyarylene, procede de synthese dudit polyarylene, polyelectrolyte solide et film conducteur de protons
CA2646944A1 (fr) * 2007-11-30 2009-05-30 Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of National Defence Composants de serum se liant a des agents de menace
KR100978055B1 (ko) 2008-08-25 2010-08-25 한국과학기술연구원 개선된 시료 주입법을 이용한 모세관 전기영동의 검출 감도향상 방법
EP2184606B1 (fr) 2008-11-03 2015-06-24 Helena Laboratories (UK) Ltd. Électrophorèse d'immunodéplacement
FR2947632B1 (fr) * 2009-07-01 2011-11-11 Sebia Sa Analyse et dosage d'hemoglobines glyquees par electrophorese capillaire, compositions tampon et kits pour electrophorese capillaire
JP6141272B2 (ja) 2011-07-14 2017-06-07 ディージェル エレクトロシステム インコーポレイティッドＤｇｅｌ Ｅｌｅｃｔｒｏｓｙｓｔｅｍ Ｉｎｃ． 電気泳動ゲルの電気泳動耐用期間を延長するための電気泳動バッファー
EP3355058B1 (fr) 2017-01-30 2019-12-25 Helena Laboratories (UK) Ltd. Nouveau test de surveillance de maladie rénale
EP3679364A1 (fr) * 2017-09-07 2020-07-15 Bristol-Myers Squibb Company Procédé de détermination de pureté par électrophorèse capillaire
WO2021154854A1 (fr) * 2020-01-27 2021-08-05 Bristol-Myers Squibb Company Matrice d'échantillon à focalisation isoélectrique
JP2024064687A (ja) 2022-10-28 2024-05-14 アークレイ株式会社 試料分析方法、キャピラリ電気泳動用溶液、及び試料分析用キット
JP2024064686A (ja) 2022-10-28 2024-05-14 アークレイ株式会社 試料分析方法、キャピラリ電気泳動用溶液、及び試料分析用キット
JP2024064685A (ja) 2022-10-28 2024-05-14 アークレイ株式会社 試料分析方法、試料の製造方法、アルブミン及びγ－グロブリン含有試料希釈用希釈液、及び試料分析用キット

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Publication number Priority date Publication date Assignee Title
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JPS59171849A (ja) * 1983-03-18 1984-09-28 Fuji Photo Film Co Ltd 電気泳動用媒体
US4778909A (en) * 1984-05-11 1988-10-18 Northeastern University Stable silica-based ether bonded phases for biopolymer separations
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4872865A (en) * 1985-10-09 1989-10-10 Harrington Arthritis Research Center Hypertonic solution for arthroscopic surgery
JPH0212059A (ja) * 1988-06-30 1990-01-17 Shimadzu Corp γ−アミノ酪酸分析法
US5120413A (en) * 1991-05-31 1992-06-09 Beckman Instruments, Inc. Analysis of samples utilzing capillary electrophoresis
FR2684998B1 (fr) * 1991-12-11 1994-10-28 Sebia Sa Procede de separation de la lp(a) par electrophorese, gels pour la mise en óoeuvre de ce procede, et application a la determination in vitro du risque atherogene lie a la presence de la lp(a).
US5536382A (en) 1994-05-23 1996-07-16 Advanced Molecular Systems, Inc. Capillary electrophoresis assay method useful for the determination of constituents of a clinical sample
US5599433A (en) * 1995-01-17 1997-02-04 Beckman Instruments, Inc. Capillary electrophoresis of glycosylated proteins
US5753094A (en) * 1995-09-20 1998-05-19 Beckman Instruments, Inc. Borate storage buffer and sample diluent
US5660701A (en) * 1996-02-29 1997-08-26 Bio-Rad Laboratories, Inc. Protein separations by capillary electrophoresis using amino acid-containing buffers
US5938930A (en) * 1997-09-29 1999-08-17 Bio-Rad Laboratories, Inc. Methods of improving peak resolution in reversed-phase electrochromatography
EP2278311A3 (fr) * 1999-05-14 2011-07-27 Waters Technologies Corporation Agents tensioactifs destructibles et leurs utilisations
FR2819889B1 (fr) * 2001-01-19 2003-08-01 Sebia Sa Procede d'electrophorese capillaire en solution libre a ph alcalin

Also Published As

Publication number Publication date
DK1229325T3 (en) 2016-01-18
JP2004517339A (ja) 2004-06-10
WO2002057737A2 (fr) 2002-07-25
JP4216598B2 (ja) 2009-01-28
EP1229325B1 (fr) 2015-10-07
BR0203699A (pt) 2003-01-07
BR0203699B1 (pt) 2014-12-30
HK1143862A1 (en) 2011-01-14
WO2002057737A3 (fr) 2002-12-12
FR2819888A1 (fr) 2002-07-26
US20020162744A1 (en) 2002-11-07
US7297244B2 (en) 2007-11-20
CN101799448B (zh) 2013-06-12
ES2557740T3 (es) 2016-01-28
CN101799448A (zh) 2010-08-11
PT1229325E (pt) 2016-02-04
CN1630816A (zh) 2005-06-22
FR2819888B1 (fr) 2003-09-12
EP1229325A1 (fr) 2002-08-07
NZ549342A (en) 2008-01-31
CA2403190C (fr) 2008-05-06

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