CA2401855A1 - Genie genetique de la tolerance a la secheresse au moyen d'un genome de plaste - Google Patents

Genie genetique de la tolerance a la secheresse au moyen d'un genome de plaste Download PDF

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Publication number
CA2401855A1
CA2401855A1 CA002401855A CA2401855A CA2401855A1 CA 2401855 A1 CA2401855 A1 CA 2401855A1 CA 002401855 A CA002401855 A CA 002401855A CA 2401855 A CA2401855 A CA 2401855A CA 2401855 A1 CA2401855 A1 CA 2401855A1
Authority
CA
Canada
Prior art keywords
plant
vector
plastid
dna sequence
chloroplast
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002401855A
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English (en)
Inventor
Henry Daniell
Seung-Bum Lee
Myung Ok Byun
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Auburn University
University of Central Florida
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2401855A1 publication Critical patent/CA2401855A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis

Abstract

L'invention concerne un procédé pour conférer l'osmoprotéction aux végétaux. On transforme des génomes de plaste végétal, notamment le génome du chloroplaste, pour exprimer un osmoprotécteur. Les plantes transgéniques et leur descendance manifestent une résistance à la sécheresse; de plus, ces plantes transgéniques ne manifestent aucun effet pléiotropique négatif tel que la stérilité ou la croissance freinée.
CA002401855A 2000-02-29 2001-02-28 Genie genetique de la tolerance a la secheresse au moyen d'un genome de plaste Abandoned CA2401855A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US18565800P 2000-02-29 2000-02-29
US60/185,658 2000-02-29
PCT/US2001/006271 WO2001064850A1 (fr) 2000-02-29 2001-02-28 Genie genetique de la tolerance a la secheresse au moyen d'un genome de plaste

Publications (1)

Publication Number Publication Date
CA2401855A1 true CA2401855A1 (fr) 2001-09-07

Family

ID=22681915

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002401855A Abandoned CA2401855A1 (fr) 2000-02-29 2001-02-28 Genie genetique de la tolerance a la secheresse au moyen d'un genome de plaste

Country Status (4)

Country Link
EP (1) EP1263934A4 (fr)
AU (1) AU2001241809A1 (fr)
CA (1) CA2401855A1 (fr)
WO (1) WO2001064850A1 (fr)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100251425A9 (en) 1998-05-15 2010-09-30 University Of Central Florida Expression of human interferon in transgenic chloroplasts
WO2004005521A1 (fr) 2002-07-03 2004-01-15 University Of Central Florida Expression de igf-1 humain dans des plastes transgeniques
KR100440725B1 (ko) 2002-06-20 2004-07-15 주식회사 그린진 바이오텍 비생물성 스트레스에 대한 단자엽 식물의 내성을증가시키는 방법
DE50310378D1 (de) 2002-07-26 2008-10-02 Basf Plant Science Gmbh Neue selektionsverfahren
WO2004044143A2 (fr) * 2002-11-06 2004-05-27 Cornell Research Foundation, Inc. Genes chimeres et transformants vegetaux de la tps
EP2261364A3 (fr) 2005-05-27 2011-03-30 The University of Central Florida, Chloroplastes génétiquement modifiés pour exprimer des protéines pharmaceutiques
EP2141981B1 (fr) 2007-03-30 2016-10-26 The Trustees Of The University Of Pennsylvania Chloroplastes génétiquement modifiés afin d'exprimer des protéines pharmaceutiques dans des plantes comestibles
US8314222B2 (en) * 2007-10-05 2012-11-20 Sapphire Energy, Inc. System for capturing and modifying large pieces of genomic DNA and constructing organisms with chloroplasts
CA2713755A1 (fr) 2008-02-07 2009-08-13 Ceregene, Inc. Preservation de photorecepteurs par administration intravitreenne d'un vecteur d'expression codant une proteine therapeutique
US10689633B2 (en) 2008-02-29 2020-06-23 The Trustees Of The University Of Pennsylvania Expression of β-mannanase in chloroplasts and its utilization in lignocellulosic woody biomass hydrolysis
WO2010033275A2 (fr) * 2008-05-27 2010-03-25 University Of Central Florida Research Foundation, Inc. Vaccin contre yersinia pestis pouvant être administré par voie orale
CN101289514B (zh) * 2008-06-13 2011-01-19 北京北方杰士生物科技有限责任公司 一种培育耐逆植物的方法及其专用dna片段
CA2780362C (fr) 2009-11-09 2019-12-24 University Of Central Florida Research Foundation, Inc. Administration par voie orale d'agents de tolerance exprimes chez les plantes
WO2013063049A1 (fr) 2011-10-24 2013-05-02 University Of Central Florida Research Foundation, Inc. Sous-motif d'exendine 4 de la toxine cholérique b exprimé dans un plastide administré par voie orale pour le traitement du diabète de type 2
WO2015039272A1 (fr) * 2013-09-17 2015-03-26 创世纪转基因技术有限公司 Tréhalose synthase tps-2 de coton, gène codant et utilisation correspondants

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5693507A (en) * 1988-09-26 1997-12-02 Auburn University Genetic engineering of plant chloroplasts
CA2087036A1 (fr) * 1991-05-09 1992-11-10 Mitchell C. Tarczynski Plants transgeniques a teneur modifiee en polyol
US5792921A (en) * 1992-02-14 1998-08-11 Londesborough; John Increasing the trehalose content of organisms by transforming them with combinations of the structural genes for trehalose synthase
US5780709A (en) * 1993-08-25 1998-07-14 Dekalb Genetics Corporation Transgenic maize with increased mannitol content
CN1262661C (zh) * 1997-08-07 2006-07-05 奥本大学 通用的叶绿体整合和表达载体、其转化植物及产物
KR20010082520A (ko) * 1998-03-11 2001-08-30 한스 루돌프 하우스, 헨리테 브룬너, 베아트리체 귄터 식물에서 트레할로즈 생합성 유전자의 발현

Also Published As

Publication number Publication date
WO2001064850A1 (fr) 2001-09-07
EP1263934A1 (fr) 2002-12-11
EP1263934A4 (fr) 2004-05-06
AU2001241809A1 (en) 2001-09-12

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FZDE Discontinued