CA2395391C - Pyrophosphorolysis activated polymerization (pap): application to allele-specific amplification and nucleic acid sequence determination - Google Patents

Pyrophosphorolysis activated polymerization (pap): application to allele-specific amplification and nucleic acid sequence determination Download PDF

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Publication number
CA2395391C
CA2395391C CA2395391A CA2395391A CA2395391C CA 2395391 C CA2395391 C CA 2395391C CA 2395391 A CA2395391 A CA 2395391A CA 2395391 A CA2395391 A CA 2395391A CA 2395391 C CA2395391 C CA 2395391C
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Prior art keywords
nucleic acid
oligonucleotide
terminus
strand
extendable
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Expired - Fee Related
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CA2395391A
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English (en)
French (fr)
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CA2395391A1 (en
Inventor
Qiang Liu
Steve S. Sommer
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City of Hope
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City of Hope
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
  • Polymers With Sulfur, Phosphorus Or Metals In The Main Chain (AREA)
  • Enzymes And Modification Thereof (AREA)
CA2395391A 2000-02-23 2001-02-22 Pyrophosphorolysis activated polymerization (pap): application to allele-specific amplification and nucleic acid sequence determination Expired - Fee Related CA2395391C (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US18431500P 2000-02-23 2000-02-23
US60/184,315 2000-02-23
US18703500P 2000-03-06 2000-03-06
US60/187,035 2000-03-06
US23718000P 2000-10-03 2000-10-03
US60/237,180 2000-10-03
PCT/US2001/005527 WO2001062975A2 (en) 2000-02-23 2001-02-22 Pyrophosphorolysis activated polymerization (pap): application to allele-specific amplification and nucleic acid sequence determination

Publications (2)

Publication Number Publication Date
CA2395391A1 CA2395391A1 (en) 2001-08-30
CA2395391C true CA2395391C (en) 2011-08-02

Family

ID=27391815

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2395391A Expired - Fee Related CA2395391C (en) 2000-02-23 2001-02-22 Pyrophosphorolysis activated polymerization (pap): application to allele-specific amplification and nucleic acid sequence determination

Country Status (9)

Country Link
US (2) US6534269B2 (enExample)
EP (2) EP1259646B1 (enExample)
JP (1) JP4989004B2 (enExample)
AT (1) ATE441726T1 (enExample)
AU (2) AU2001241621B2 (enExample)
CA (1) CA2395391C (enExample)
DE (1) DE60139762D1 (enExample)
ES (1) ES2330408T3 (enExample)
WO (1) WO2001062975A2 (enExample)

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CN110603326B (zh) * 2017-09-26 2024-03-08 宁波尚宁生物技术有限公司 扩增靶核酸的方法
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ES2969883T3 (es) 2018-03-21 2024-05-23 Hoffmann La Roche ADN polimerasas para la incorporación eficiente y eficaz de dNTP metilados
US11489136B2 (en) 2018-05-07 2022-11-01 Oti Lumionics Inc. Method for providing an auxiliary electrode and device including an auxiliary electrode
US11268140B2 (en) * 2018-06-12 2022-03-08 Shaofeng Ding Delayed pyrophosphorolysis activated polymerization
JP2022508040A (ja) 2018-11-23 2022-01-19 オーティーアイ ルミオニクス インコーポレーテッド 光透過領域を含むオプトエレクトロニクスデバイス
CN116456753A (zh) 2019-03-07 2023-07-18 Oti照明公司 一种光电子器件
KR20250110358A (ko) 2019-04-18 2025-07-18 오티아이 루미오닉스 인크. 핵 생성 억제 코팅 형성용 물질 및 이를 포함하는 디바이스
WO2020225778A1 (en) 2019-05-08 2020-11-12 Oti Lumionics Inc. Materials for forming a nucleation-inhibiting coating and devices incorporating same
CN114097102B (zh) 2019-06-26 2023-11-03 Oti照明公司 包括具有光衍射特征的光透射区域的光电设备
US11832473B2 (en) 2019-06-26 2023-11-28 Oti Lumionics Inc. Optoelectronic device including light transmissive regions, with light diffraction characteristics
WO2021028800A1 (en) 2019-08-09 2021-02-18 Oti Lumionics Inc. Opto-electronic device including an auxiliary electrode and a partition
JP7403883B2 (ja) 2019-12-24 2023-12-25 オーティーアイ ルミオニクス インコーポレーテッド キャッピング層を含む発光デバイスおよびそれを製造するための方法
CN111108860B (zh) * 2020-01-19 2024-05-14 黑龙江丰沃非凡农业科技发展有限公司 一种仿形播种机
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Also Published As

Publication number Publication date
US20020127554A1 (en) 2002-09-12
WO2001062975A9 (en) 2002-10-24
US7105298B2 (en) 2006-09-12
JP2003526344A (ja) 2003-09-09
US6534269B2 (en) 2003-03-18
WO2001062975A2 (en) 2001-08-30
WO2001062975A3 (en) 2002-08-29
EP2112233A1 (en) 2009-10-28
EP1259646A2 (en) 2002-11-27
AU4162101A (en) 2001-09-03
EP1259646B1 (en) 2009-09-02
EP2112233B1 (en) 2013-10-23
JP4989004B2 (ja) 2012-08-01
US20030092051A1 (en) 2003-05-15
CA2395391A1 (en) 2001-08-30
AU2001241621B2 (en) 2005-08-25
DE60139762D1 (de) 2009-10-15
ATE441726T1 (de) 2009-09-15
ES2330408T3 (es) 2009-12-10

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