CA2394275A1 - Compositions et procede permettant de realiser des reactions biologiques - Google Patents

Compositions et procede permettant de realiser des reactions biologiques Download PDF

Info

Publication number
CA2394275A1
CA2394275A1 CA002394275A CA2394275A CA2394275A1 CA 2394275 A1 CA2394275 A1 CA 2394275A1 CA 002394275 A CA002394275 A CA 002394275A CA 2394275 A CA2394275 A CA 2394275A CA 2394275 A1 CA2394275 A1 CA 2394275A1
Authority
CA
Canada
Prior art keywords
hybridization
layer
array
substrate
adhesive
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002394275A
Other languages
English (en)
Inventor
George W. Hawkins
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Sweden AB
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/464,490 external-priority patent/US6589778B1/en
Application filed by Individual filed Critical Individual
Publication of CA2394275A1 publication Critical patent/CA2394275A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00702—Processes involving means for analysing and characterising the products
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0654—Lenses; Optical fibres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0822—Slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1816—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using induction heating
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B01L2400/049—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics vacuum
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
    • C07B2200/11—Compounds covalently bound to a solid support
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)

Abstract

La présente invention concerne un dispositif permettant de réaliser des réactions biologiques. D'une manière spécifique, l'invention concerne un dispositif permettant de réaliser des réactions d'hybridation de l'acide nucléique sur une couche substrat possédant plusieurs sites de liaison d'oligonucléotides, au moyen de substrats dotés de couvertures souples.
CA002394275A 1999-12-15 2000-12-15 Compositions et procede permettant de realiser des reactions biologiques Abandoned CA2394275A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US09/464,490 1999-12-15
US09/464,490 US6589778B1 (en) 1999-12-15 1999-12-15 Method and apparatus for performing biological reactions on a substrate surface
US60576600A 2000-06-28 2000-06-28
US09/605,766 2000-06-28
PCT/US2000/034145 WO2001044515A2 (fr) 1999-12-15 2000-12-15 Compositions et procede permettant de realiser des reactions biologiques

Publications (1)

Publication Number Publication Date
CA2394275A1 true CA2394275A1 (fr) 2001-06-21

Family

ID=27040998

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002394275A Abandoned CA2394275A1 (fr) 1999-12-15 2000-12-15 Compositions et procede permettant de realiser des reactions biologiques

Country Status (5)

Country Link
EP (1) EP1242819A2 (fr)
JP (1) JP2003517156A (fr)
AU (1) AU778696B2 (fr)
CA (1) CA2394275A1 (fr)
WO (1) WO2001044515A2 (fr)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE0004296D0 (sv) * 2000-11-23 2000-11-23 Gyros Ab Device and method for the controlled heating in micro channel systems
EP2169383A1 (fr) * 2001-06-15 2010-03-31 Bayer Technology Services GmbH Organe pour l'écoulement à travers des cuvettes et son utilisation
AU2002367840A1 (en) * 2001-07-26 2003-11-17 Motorola, Inc. System and methods for mixing within a microfluidic device
CZ297082B6 (cs) * 2002-06-03 2006-09-13 Ing. Ilja Krejcí-Engineering Soucástky s trojrozmernou strukturou pripravené tlustovrstvou technologií a zpusob jejich výroby
US20030235521A1 (en) * 2002-06-21 2003-12-25 Shea Laurence R. Array assay devices and methods of using the same
JP4057967B2 (ja) * 2002-07-31 2008-03-05 株式会社東芝 塩基配列自動解析装置
US7745203B2 (en) 2002-07-31 2010-06-29 Kabushiki Kaisha Toshiba Base sequence detection apparatus and base sequence automatic analyzing apparatus
US6913931B2 (en) 2002-10-03 2005-07-05 3M Innovative Properties Company Devices, methods and systems for low volume microarray processing
US20040101439A1 (en) * 2002-11-21 2004-05-27 Fusco Adam J Biological and chemical reaction devices and methods of manufacture
WO2005016532A2 (fr) * 2003-06-13 2005-02-24 Corning Incorporated Systeme automatise de chambre de reaction pour essais biologiques
DE10340473B4 (de) * 2003-09-03 2007-08-23 Eppendorf Ag Abdeckung einer Hybridisierungskammer
KR100601936B1 (ko) * 2003-12-17 2006-07-14 삼성전자주식회사 접착부 지지체와 2이상의 접착물질을 갖는 마이크로어레이반응 챔버용 패취
DE102004022263A1 (de) * 2004-05-06 2005-12-15 Clondiag Chip Technologies Gmbh Vorrichtung und Verfahren zum Nachweis von molekularen Wechselwirkungen
JP4732135B2 (ja) * 2005-11-10 2011-07-27 キヤノン株式会社 反応装置
JP4705504B2 (ja) * 2006-04-05 2011-06-22 アイダエンジニアリング株式会社 マイクロ流体チップ
PE20090965A1 (es) * 2007-10-12 2009-07-13 Bigtec Private Ltd Micro chip
US20100071614A1 (en) * 2008-09-22 2010-03-25 Momentive Performance Materials, Inc. Fluid distribution apparatus and method of forming the same
JP2010236969A (ja) * 2009-03-31 2010-10-21 Hitachi High-Technologies Corp 核酸分析デバイス，核酸分析装置、及び核酸分析方法
JP6094393B2 (ja) * 2013-06-13 2017-03-15 住友ベークライト株式会社 被覆体、及び被覆体の使用方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5229297A (en) * 1989-02-03 1993-07-20 Eastman Kodak Company Containment cuvette for PCR and method of use
EP0695941B1 (fr) * 1994-06-08 2002-07-31 Affymetrix, Inc. Procédé et appareil d'emballage de puces
US5856174A (en) * 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
EP1291441A3 (fr) * 1995-09-12 2003-03-19 Becton, Dickinson and Company Dispositif et procédé d'amplification et de dosage d'ADN
US5907703A (en) * 1996-05-08 1999-05-25 Mijenix Corporation Device driver for accessing computer files
AU1517999A (en) * 1997-10-15 1999-05-03 Aclara Biosciences, Inc. Laminate microstructure device and method for making same
NZ506204A (en) * 1998-02-20 2003-06-30 Nanogen Inc Advanced active devices and methods for molecular biological analysis and diagnostics

Also Published As

Publication number Publication date
WO2001044515A2 (fr) 2001-06-21
AU3264101A (en) 2001-06-25
WO2001044515A3 (fr) 2002-03-07
AU778696B2 (en) 2004-12-16
EP1242819A2 (fr) 2002-09-25
WO2001044515A9 (fr) 2002-07-04
JP2003517156A (ja) 2003-05-20

Similar Documents

Publication Publication Date Title
AU2001234593B2 (en) Compositions and methods for performing biological reactions
US20030157503A1 (en) Compositions and methods for performing biological reactions
AU2001234593A1 (en) Compositions and methods for performing biological reactions
AU778696B2 (en) Compositions and methods for performing biological reactions
US6875619B2 (en) Microfluidic devices comprising biochannels
US6589778B1 (en) Method and apparatus for performing biological reactions on a substrate surface
US20050009101A1 (en) Microfluidic devices comprising biochannels
JP6654681B2 (ja) 試料導入から結果出力までのプロセス化を提供する単一構造バイオチップおよび製造方法
US6605454B2 (en) Microfluidic devices with monolithic microwave integrated circuits
US9987636B2 (en) Method and apparatus for use in temperature controlled processing of microfluidic samples
US9086371B2 (en) Fluidics devices
EP2872892B1 (fr) Sensor adn support flexible et procédé pour son utilisation
US8697007B2 (en) Biodetection cassette with automated actuator
CA2393690A1 (fr) Dispositifs microfluidiques multicouches pour reactions de substances a analyser
US20030138969A1 (en) Closed substrate platforms suitable for analysis of biomolecules
WO2002052045A1 (fr) Plate-formes actives et biocompatibles preparees par polymerisation de films de revetement de surface
CA2393733A1 (fr) Procedes et compositions se rapportant a la detection electrique des reactions d'acides nucleiques
AU2002360499A1 (en) Microfluidic device and surface decoration process for solid phase affinity binding assays
EP1415715A1 (fr) Système de réseaux moléculaires multiples
US20030209432A1 (en) Methods and compositions relating to electrical detection of nucleic acid reactions
CN111944682A (zh) 一种核酸检测芯片、制备方法及核酸检测方法
US20030134410A1 (en) Compositions and methods for performing biological reactions
WO2011088588A1 (fr) Réacteur utilisable en vue d'une analyse quantitative d'acides nucléiques
US20230100295A1 (en) System and method for rapid multiplexed sample processing with applications for nucleic acid amplification assays

Legal Events

Date Code Title Description
FZDE Discontinued