CA2392239A1 - Use of fluorescence correlation spectroscopy to identify compounds that bind to target species under isothermal denaturing conditions - Google Patents
Use of fluorescence correlation spectroscopy to identify compounds that bind to target species under isothermal denaturing conditions Download PDFInfo
- Publication number
- CA2392239A1 CA2392239A1 CA002392239A CA2392239A CA2392239A1 CA 2392239 A1 CA2392239 A1 CA 2392239A1 CA 002392239 A CA002392239 A CA 002392239A CA 2392239 A CA2392239 A CA 2392239A CA 2392239 A1 CA2392239 A1 CA 2392239A1
- Authority
- CA
- Canada
- Prior art keywords
- target species
- test
- denaturation
- test compound
- isothermal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
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- 238000002060 fluorescence correlation spectroscopy Methods 0.000 title claims abstract description 30
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- 238000000034 method Methods 0.000 claims abstract description 73
- 238000012360 testing method Methods 0.000 claims abstract description 59
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- 235000011008 sodium phosphates Nutrition 0.000 description 1
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- 238000009987 spinning Methods 0.000 description 1
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- FRGKKTITADJNOE-UHFFFAOYSA-N sulfanyloxyethane Chemical compound CCOS FRGKKTITADJNOE-UHFFFAOYSA-N 0.000 description 1
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- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/805—Optical property
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/12—Condition responsive control
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/13—Tracers or tags
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/458,172 | 1999-12-09 | ||
| US09/458,172 US6582907B1 (en) | 1999-12-09 | 1999-12-09 | Use of fluorescence correlation spectroscopy to identify compounds that bind to target species under isothermal denaturing conditions |
| PCT/US2000/042642 WO2001044817A2 (en) | 1999-12-09 | 2000-12-07 | Fluorescence correlation spectroscopy to identify compound-target binding |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2392239A1 true CA2392239A1 (en) | 2001-06-21 |
Family
ID=23819671
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002392239A Abandoned CA2392239A1 (en) | 1999-12-09 | 2000-12-07 | Use of fluorescence correlation spectroscopy to identify compounds that bind to target species under isothermal denaturing conditions |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US6582907B1 (cg-RX-API-DMAC7.html) |
| EP (1) | EP1236047B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP2003517609A (cg-RX-API-DMAC7.html) |
| AT (1) | ATE265684T1 (cg-RX-API-DMAC7.html) |
| AU (1) | AU777163B2 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2392239A1 (cg-RX-API-DMAC7.html) |
| DE (1) | DE60010323T2 (cg-RX-API-DMAC7.html) |
| DK (1) | DK1236047T3 (cg-RX-API-DMAC7.html) |
| ES (1) | ES2218280T3 (cg-RX-API-DMAC7.html) |
| HK (1) | HK1047158B (cg-RX-API-DMAC7.html) |
| PT (1) | PT1236047E (cg-RX-API-DMAC7.html) |
| TR (1) | TR200401075T4 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2001044817A2 (cg-RX-API-DMAC7.html) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9425138D0 (en) * | 1994-12-12 | 1995-02-08 | Dynal As | Isolation of nucleic acid |
| US6517781B1 (en) * | 1997-06-02 | 2003-02-11 | Aurora Biosciences Corporation | Low fluorescence assay platforms and related methods for drug discovery |
| DE10013854A1 (de) * | 2000-03-17 | 2001-09-20 | Zeiss Carl Jena Gmbh | Anordnung und Verfahren zur Untersuchung von Wirkstoffen zur Beeinflussung intrazellulärer Vorgänge |
| AU2003202026A1 (en) | 2002-01-16 | 2003-09-02 | Dynal Biotech Asa | Method for isolating nucleic acids and protein from a single sample |
| CN1662810A (zh) | 2002-06-21 | 2005-08-31 | 奥林巴斯株式会社 | 生物分子分析装置 |
| EP1546675A4 (en) * | 2002-08-01 | 2008-03-26 | Sensor Technologies Inc | METHOD FOR MEASURING MOLECULAR INTERACTIONS |
| GB0229287D0 (en) * | 2002-12-16 | 2003-01-22 | Dna Res Innovations Ltd | Polyfunctional reagents |
| WO2006013109A1 (en) * | 2004-08-06 | 2006-02-09 | Epfl Swiss Federal Institute Of Technology-Lausanne | Method for testing a substance interacting with a target molecule |
| US7705987B2 (en) * | 2005-02-15 | 2010-04-27 | Tata Institute Of Fundamental Research | Fluorescence correlation microscopy with real-time alignment readout |
| DE102007045666A1 (de) * | 2007-09-25 | 2009-04-02 | Carl Zeiss Microimaging Gmbh | Verfahren und Anordnung zur Ausgabe von Restfehlern einer an eine Punktemenge angepassten Funktion |
| KR100963305B1 (ko) | 2008-01-23 | 2010-06-11 | 건국대학교 산학협력단 | 단백질 내의 도메인 간 변성과정 분석방법 |
| US12400741B2 (en) * | 2020-02-28 | 2025-08-26 | Howard University | Selective extraction of late exponentials |
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| US5585277A (en) | 1993-06-21 | 1996-12-17 | Scriptgen Pharmaceuticals, Inc. | Screening method for identifying ligands for target proteins |
| US5679582A (en) | 1993-06-21 | 1997-10-21 | Scriptgen Pharmaceuticals, Inc. | Screening method for identifying ligands for target proteins |
| CA2184195C (en) | 1995-10-25 | 2002-04-16 | Andrew Pakula | Screening method for identifying ligands for target proteins |
| CA2236339A1 (en) | 1995-12-07 | 1997-06-12 | Robert T. Sauer | A fluorescence-based screening method for identifying ligands |
| KR20000011069A (ko) | 1996-05-09 | 2000-02-25 | 스리-디멘셔널 파마슈티컬스 인코포레이티드 | 리간드 개발 및 다변수 단백질 화학의 최적화를 위한미량이식판 열 이동 분석 및 그 장치 |
| EP0836090A1 (en) | 1996-10-12 | 1998-04-15 | Evotec BioSystems GmbH | Method of analysis of samples by determination of the distribution of specific brightnesses of particles |
| WO1999029894A1 (en) | 1997-12-05 | 1999-06-17 | Pharmacia & Upjohn Company | Fluorescence-based high throughput screening assays for protein kinases and phosphatases |
| US6242190B1 (en) | 1999-12-01 | 2001-06-05 | John Hopkins University | Method for high throughput thermodynamic screening of ligands |
| US6376180B1 (en) * | 1999-12-09 | 2002-04-23 | Pharmacia & Upjohn Company | Methods of identifying compounds that bind to target species under isothermal denaturing conditions |
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- 2000-12-07 JP JP2001545854A patent/JP2003517609A/ja active Pending
- 2000-12-07 TR TR2004/01075T patent/TR200401075T4/xx unknown
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| EP1236047B1 (en) | 2004-04-28 |
| AU4519801A (en) | 2001-06-25 |
| DE60010323D1 (de) | 2004-06-03 |
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| JP2003517609A (ja) | 2003-05-27 |
| ATE265684T1 (de) | 2004-05-15 |
| US20030219820A1 (en) | 2003-11-27 |
| WO2001044817A3 (en) | 2002-05-10 |
| PT1236047E (pt) | 2004-08-31 |
| DK1236047T3 (da) | 2004-07-05 |
| DE60010323T2 (de) | 2005-05-25 |
| US6582907B1 (en) | 2003-06-24 |
| TR200401075T4 (tr) | 2004-07-21 |
| AU777163B2 (en) | 2004-10-07 |
| WO2001044817A2 (en) | 2001-06-21 |
| HK1047158A1 (en) | 2003-02-07 |
| ES2218280T3 (es) | 2004-11-16 |
| HK1047158B (en) | 2004-12-03 |
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