CA2391626A1 - Criblage combinatoire de populations mixtes d'organismes - Google Patents

Criblage combinatoire de populations mixtes d'organismes Download PDF

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Publication number
CA2391626A1
CA2391626A1 CA002391626A CA2391626A CA2391626A1 CA 2391626 A1 CA2391626 A1 CA 2391626A1 CA 002391626 A CA002391626 A CA 002391626A CA 2391626 A CA2391626 A CA 2391626A CA 2391626 A1 CA2391626 A1 CA 2391626A1
Authority
CA
Canada
Prior art keywords
biomolecule
bioactivity
nucleic acid
library
interest
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002391626A
Other languages
English (en)
Inventor
Jay M. Short
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF Enzymes LLC
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/663,620 external-priority patent/US7018793B1/en
Application filed by Individual filed Critical Individual
Publication of CA2391626A1 publication Critical patent/CA2391626A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention concerne une méthode permettant de cribler des bibliothèques génétiques dérivées d'une population mixte d'organismes afin de détecter une bioactivité d'une biomolécule d'intérêt. La population mixte d'organismes peut être une population cultivée ou une population non cultivée issue, par exemple, de l'environnement. L'invention concerne également des méthodes permettant de cribler des isolats ou des populations enrichies d'organismes, lesquels isolats comprennent une population qui est spatialement ou temporellement hiérarchisée, et qui fait partie, par exemple, d'une espèce, d'une genre, d'une famille ou d'une classe particulières d'organismes. Des clones identifiés contenant une biomolécule ou présentant une bioactivité d'intérêt peuvent être panachés ou l'ADN contenu dans ledit clone peut être panaché afin de créer de nouvelles biomolécules ou bioactivités d'intérêt.
CA002391626A 2000-09-15 2001-09-17 Criblage combinatoire de populations mixtes d'organismes Abandoned CA2391626A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/663,620 US7018793B1 (en) 1995-12-07 2000-09-15 Combinatorial screening of mixed populations of organisms
US09/663,620 2000-09-15
PCT/US2001/029712 WO2002022810A2 (fr) 2000-09-15 2001-09-17 Criblage combinatoire de populations mixtes d'organismes

Publications (1)

Publication Number Publication Date
CA2391626A1 true CA2391626A1 (fr) 2002-03-21

Family

ID=24662600

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002391626A Abandoned CA2391626A1 (fr) 2000-09-15 2001-09-17 Criblage combinatoire de populations mixtes d'organismes

Country Status (5)

Country Link
EP (1) EP1319068A2 (fr)
JP (1) JP2004524811A (fr)
AU (1) AU9120801A (fr)
CA (1) CA2391626A1 (fr)
WO (1) WO2002022810A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102690799A (zh) 2000-12-01 2012-09-26 维莱尼姆公司 具有脱卤素酶活性的酶及其使用方法
AU2006235875B8 (en) * 2000-12-01 2011-04-14 Basf Enzymes Llc Enzymes having dehalogenase activity and methods of use thereof
CN102590528A (zh) * 2012-01-18 2012-07-18 厦门大学 一种蛋白相互作用的检测方法
CN106124471B (zh) * 2016-07-19 2019-04-16 天津大学 时域荧光寿命成像结构及寿命测试获取方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6117679A (en) * 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
GB9701425D0 (en) * 1997-01-24 1997-03-12 Bioinvent Int Ab A method for in vitro molecular evolution of protein function

Also Published As

Publication number Publication date
EP1319068A2 (fr) 2003-06-18
WO2002022810A3 (fr) 2003-02-27
AU9120801A (en) 2002-03-26
WO2002022810A2 (fr) 2002-03-21
JP2004524811A (ja) 2004-08-19

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued