CA2385172A1 - Mutants de la sous-unite b de la toxine ab5 aux caracteristiques de conjugaison chimique modifiees - Google Patents

Mutants de la sous-unite b de la toxine ab5 aux caracteristiques de conjugaison chimique modifiees Download PDF

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Publication number
CA2385172A1
CA2385172A1 CA002385172A CA2385172A CA2385172A1 CA 2385172 A1 CA2385172 A1 CA 2385172A1 CA 002385172 A CA002385172 A CA 002385172A CA 2385172 A CA2385172 A CA 2385172A CA 2385172 A1 CA2385172 A1 CA 2385172A1
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CA
Canada
Prior art keywords
protein
subunit
toxin
amino acid
recombinant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002385172A
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English (en)
Inventor
Harold H. Handley
Tapio Haaparanta
Karla L. Ewalt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Active Biotech AB
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2385172A1 publication Critical patent/CA2385172A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/235—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bordetella (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/25—Shigella (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/28—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Vibrionaceae (F)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Oncology (AREA)
  • Transplantation (AREA)
  • Obesity (AREA)
  • Hematology (AREA)
  • Communicable Diseases (AREA)
  • Emergency Medicine (AREA)
  • Pain & Pain Management (AREA)
  • Rheumatology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention porte sur une protéine de recombinaison de la sous-unité B de AB¿5? comprenant au moins une mutation. Cette mutation modifie la quantité des restes d'acides aminés disponibles pour la modification chimique en comparaison avec une protéine de la sous-unité B de AB¿5? de génotype sauvage. Cette protéine de recombinaison conserve une affinité de liaison efficace du ligand cible. Par exemple, des mutations de détermination spécifique sont produites dans la sous-unité B de la toxine du choléra (CTB) de sorte qu'elle puisse toujours se lier avec une haute affinité à son récepteur, Gm-1, mais puisse être liée de manière covalente et spécifique au niveau des lysines ou cystéines à un immunogène ou vaccin. Le vaccin obtenu à partir de cette liaison est un vaccin contre l'infection des muqueuses qui a une immunogénicité élevée du fait de son interaction avec la CTB. Ce vaccin peut être produit facilement et à faible coût. En variante, l'invention porte également sur une technique de traitement de la CTB de sorte qu'il n'y ait pas de fixation par liaison non covalente avec un vaccin ou un immunogène.
CA002385172A 1999-10-08 2000-10-05 Mutants de la sous-unite b de la toxine ab5 aux caracteristiques de conjugaison chimique modifiees Abandoned CA2385172A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US15856199P 1999-10-08 1999-10-08
US60/158,561 1999-10-08
PCT/US2000/027607 WO2001027144A2 (fr) 1999-10-08 2000-10-05 Mutants de la sous-unite b de la toxine ab5 aux caracteristiques de conjugaison chimique modifiees

Publications (1)

Publication Number Publication Date
CA2385172A1 true CA2385172A1 (fr) 2001-04-19

Family

ID=22568704

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002385172A Abandoned CA2385172A1 (fr) 1999-10-08 2000-10-05 Mutants de la sous-unite b de la toxine ab5 aux caracteristiques de conjugaison chimique modifiees

Country Status (7)

Country Link
EP (1) EP1222202A2 (fr)
JP (1) JP2003511061A (fr)
AU (1) AU7865900A (fr)
CA (1) CA2385172A1 (fr)
IL (1) IL149009A0 (fr)
NZ (1) NZ518342A (fr)
WO (1) WO2001027144A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0115382D0 (en) * 2001-06-22 2001-08-15 Univ Bristol Mutant
EP1456651B1 (fr) 2001-11-30 2008-05-07 National Research Council Of Canada Nouvelles molecules d'auto-assemblage
US7455843B2 (en) 2003-09-25 2008-11-25 Research Foundation Of State University Of New York, The Adjuvant activities of mutants of LT-IIa and LT-IIb enterotoxin lacking binding to ganglioside
EP1934246B8 (fr) * 2005-10-07 2012-02-08 Istituto di Ricerche di Biologia Molecolare P. Angeletti S.R.L. Vaccin de metalloproteinase 11 matricielle
KR101303282B1 (ko) 2010-11-08 2013-09-03 한국생명공학연구원 Ａｂ타입 세균 독소의 톡신 ｂ－서브유닛 결손에 의해 ａｂ타입 세균 외독소가 결여된 균주 및 이의 용도
EP2970409A2 (fr) 2013-03-15 2016-01-20 Bioven 3 Limited Protéines de synthèse à auto-assemblage

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2636842B1 (fr) * 1988-09-27 1994-06-10 Liege Universite Etat Proteine de fusion d'une sequence derivee de la sous-unite b de la toxine cholerique et d'un antigene heterologue doue de proprietes immunogenes, compositions de vaccins les contenant et acides nucleiques recombinants contenant une sequence de nucleotides codant pour ladite proteine de fusion
JPH02115129A (ja) * 1988-10-21 1990-04-27 Kokuritsu Yobou Eisei Kenkyusho 抗ウィルス剤
US5834246A (en) * 1989-09-18 1998-11-10 Vitec Aktiebolag Recombinant systems for expression of cholera B-subunit with the aid of foreign promoters and/or leader peptides

Also Published As

Publication number Publication date
WO2001027144A3 (fr) 2002-01-17
AU7865900A (en) 2001-04-23
NZ518342A (en) 2004-04-30
EP1222202A2 (fr) 2002-07-17
IL149009A0 (en) 2002-11-10
JP2003511061A (ja) 2003-03-25
WO2001027144A2 (fr) 2001-04-19

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued