CA2375901A1 - Acquisition simultanee d'images utilisant plusieurs colorants d'echantillons fluorophores - Google Patents

Acquisition simultanee d'images utilisant plusieurs colorants d'echantillons fluorophores Download PDF

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Publication number
CA2375901A1
CA2375901A1 CA002375901A CA2375901A CA2375901A1 CA 2375901 A1 CA2375901 A1 CA 2375901A1 CA 002375901 A CA002375901 A CA 002375901A CA 2375901 A CA2375901 A CA 2375901A CA 2375901 A1 CA2375901 A1 CA 2375901A1
Authority
CA
Canada
Prior art keywords
specimen
optical signal
spectral
set forth
assembly
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002375901A
Other languages
English (en)
Inventor
Herman Deweerd
Jose D. Hernandez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Virtek Biotech Canada Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2375901A1 publication Critical patent/CA2375901A1/fr
Abandoned legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V10/00—Arrangements for image or video recognition or understanding
    • G06V10/10—Image acquisition
    • G06V10/12—Details of acquisition arrangements; Constructional details thereof
    • G06V10/14—Optical characteristics of the device performing the acquisition or on the illumination arrangements
    • G06V10/143—Sensing or illuminating at different wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels

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  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Multimedia (AREA)
  • Theoretical Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Selon cette invention, un ensemble d'instrument optique destiné à balayer des biopuces à la recherche d'échantillons d'ADN comprend un émetteur pour projeter un signal optique possédant au moins un premier et un deuxième réseaux spectraux vers un échantillon contenant un ADN. Un détecteur comprend un capteur pour détecter un signal optique émis à partir de cet échantillon. Un premier mécanisme d'entraînement déplace le signal optique en avant et en arrière sur l'échantillon. Un deuxième mécanisme d'entraînement modifie la position de l'échantillon par rapport au signal optique. Un contrôleur termine la détection d'un ou de plusieurs réseaux spectraux tout en déplaçant le signal optique vers l'avant et termine la détection de l'autre réseau spectral tout en déplaçant le signal optique vers l'arrière.
CA002375901A 1999-06-18 2000-06-16 Acquisition simultanee d'images utilisant plusieurs colorants d'echantillons fluorophores Abandoned CA2375901A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13999199P 1999-06-18 1999-06-18
US60/139,991 1999-06-18
PCT/US2000/016795 WO2000078993A1 (fr) 1999-06-18 2000-06-16 Acquisition simultanee d'images utilisant plusieurs colorants d'echantillons fluorophores

Publications (1)

Publication Number Publication Date
CA2375901A1 true CA2375901A1 (fr) 2000-12-28

Family

ID=22489229

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002375901A Abandoned CA2375901A1 (fr) 1999-06-18 2000-06-16 Acquisition simultanee d'images utilisant plusieurs colorants d'echantillons fluorophores

Country Status (4)

Country Link
AU (1) AU5877900A (fr)
CA (1) CA2375901A1 (fr)
GB (1) GB2366930B (fr)
WO (1) WO2000078993A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4381122B2 (ja) * 2003-02-14 2009-12-09 晶宇生物科技實業股▲分▼有限公司 側壁付微細アレイバイオチップ反射型画像アクセス及び分析装置、並びにその方法
EP1447454A1 (fr) * 2003-02-14 2004-08-18 DR. Chip Biotechnology Incorporation Procédé et appareil pour détecter des pathogènes

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4931223A (en) * 1986-07-24 1990-06-05 Tropix, Inc. Methods of using chemiluminescent 1,2-dioxetanes
US5817462A (en) * 1995-02-21 1998-10-06 Applied Spectral Imaging Method for simultaneous detection of multiple fluorophores for in situ hybridization and multicolor chromosome painting and banding
US6007994A (en) * 1995-12-22 1999-12-28 Yale University Multiparametric fluorescence in situ hybridization

Also Published As

Publication number Publication date
WO2000078993A1 (fr) 2000-12-28
GB0128260D0 (en) 2002-01-16
AU5877900A (en) 2001-01-09
GB2366930A (en) 2002-03-20
GB2366930B (en) 2003-11-19

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Legal Events

Date Code Title Description
FZDE Discontinued