GB2366930A - Simultaneous image acquisition using multiple fluorophore probe dyes - Google Patents

Simultaneous image acquisition using multiple fluorophore probe dyes

Info

Publication number
GB2366930A
GB2366930A GB0128260A GB0128260A GB2366930A GB 2366930 A GB2366930 A GB 2366930A GB 0128260 A GB0128260 A GB 0128260A GB 0128260 A GB0128260 A GB 0128260A GB 2366930 A GB2366930 A GB 2366930A
Authority
GB
United Kingdom
Prior art keywords
optical signal
specimen
image acquisition
simultaneous image
multiple fluorophore
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB0128260A
Other versions
GB2366930B (en
GB0128260D0 (en
Inventor
Herman Deweerd
Jose D Hernandez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Virtek Vision Corp
Original Assignee
Virtek Vision Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Virtek Vision Corp filed Critical Virtek Vision Corp
Publication of GB0128260D0 publication Critical patent/GB0128260D0/en
Publication of GB2366930A publication Critical patent/GB2366930A/en
Application granted granted Critical
Publication of GB2366930B publication Critical patent/GB2366930B/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V10/00—Arrangements for image or video recognition or understanding
    • G06V10/10—Image acquisition
    • G06V10/12—Details of acquisition arrangements; Constructional details thereof
    • G06V10/14—Optical characteristics of the device performing the acquisition or on the illumination arrangements
    • G06V10/143—Sensing or illuminating at different wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels

Landscapes

  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Multimedia (AREA)
  • Theoretical Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

An optical instrument assembly for scanning biochips for DNA samples includes a transmitter for projecting an optical signal having at least a first and a second spectral array onto a DNA containing specimen. A detector includes a sensor for detecting an emitted optical signal from the specimen. A first drive mechanism varies the position of the optical signal on the specimen in a forward and a reverse direction. A second drive mechanism varies the position of the specimen relative to the optical signal. A controller terminates detection of one of the spectral arrays while varying the position of the optical signal in the forward direction and terminates detection of the other spectral array while varying the position of the optical signal in the reverse direction.
GB0128260A 1999-06-18 2000-06-16 Simultaneous image acquisition using multiple fluorophore probe dyes Expired - Fee Related GB2366930B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13999199P 1999-06-18 1999-06-18
PCT/US2000/016795 WO2000078993A1 (en) 1999-06-18 2000-06-16 Simultaneous image acquisition using multiple fluorophore probe dyes

Publications (3)

Publication Number Publication Date
GB0128260D0 GB0128260D0 (en) 2002-01-16
GB2366930A true GB2366930A (en) 2002-03-20
GB2366930B GB2366930B (en) 2003-11-19

Family

ID=22489229

Family Applications (1)

Application Number Title Priority Date Filing Date
GB0128260A Expired - Fee Related GB2366930B (en) 1999-06-18 2000-06-16 Simultaneous image acquisition using multiple fluorophore probe dyes

Country Status (4)

Country Link
AU (1) AU5877900A (en)
CA (1) CA2375901A1 (en)
GB (1) GB2366930B (en)
WO (1) WO2000078993A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4381122B2 (en) * 2003-02-14 2009-12-09 晶宇生物科技實業股▲分▼有限公司 Micro-array biochip reflective image access and analysis device with sidewall and method thereof
EP1447454A1 (en) * 2003-02-14 2004-08-18 DR. Chip Biotechnology Incorporation Method and apparatus for detecting pathogens

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4931223A (en) * 1986-07-24 1990-06-05 Tropix, Inc. Methods of using chemiluminescent 1,2-dioxetanes
US5817462A (en) * 1995-02-21 1998-10-06 Applied Spectral Imaging Method for simultaneous detection of multiple fluorophores for in situ hybridization and multicolor chromosome painting and banding
US6007994A (en) * 1995-12-22 1999-12-28 Yale University Multiparametric fluorescence in situ hybridization

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4931223A (en) * 1986-07-24 1990-06-05 Tropix, Inc. Methods of using chemiluminescent 1,2-dioxetanes
US5817462A (en) * 1995-02-21 1998-10-06 Applied Spectral Imaging Method for simultaneous detection of multiple fluorophores for in situ hybridization and multicolor chromosome painting and banding
US6007994A (en) * 1995-12-22 1999-12-28 Yale University Multiparametric fluorescence in situ hybridization

Also Published As

Publication number Publication date
AU5877900A (en) 2001-01-09
CA2375901A1 (en) 2000-12-28
GB2366930B (en) 2003-11-19
GB0128260D0 (en) 2002-01-16
WO2000078993A1 (en) 2000-12-28

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Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee

Effective date: 20050616