CA2374649A1 - Appareil pour l'ecoulement tangentiel, la concentration et la fusion des cellules - Google Patents

Appareil pour l'ecoulement tangentiel, la concentration et la fusion des cellules Download PDF

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Publication number
CA2374649A1
CA2374649A1 CA002374649A CA2374649A CA2374649A1 CA 2374649 A1 CA2374649 A1 CA 2374649A1 CA 002374649 A CA002374649 A CA 002374649A CA 2374649 A CA2374649 A CA 2374649A CA 2374649 A1 CA2374649 A1 CA 2374649A1
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CA
Canada
Prior art keywords
cell
bladder
cells
fusion
liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002374649A
Other languages
English (en)
Inventor
Richard E. Walters
Alan D. King
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2374649A1 publication Critical patent/CA2374649A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B01D61/18—Apparatus therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B01D61/20—Accessories; Auxiliary operations
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/02—Separators
    • B03C5/022—Non-uniform field separators
    • B03C5/026—Non-uniform field separators using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion

Landscapes

  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Water Supply & Treatment (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Physics & Mathematics (AREA)
  • Electromagnetism (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention porte sur un appareil (12) utilisé pour l'écoulement tangentiel, la concentration et la fusion des cellules et comprenant un corps (24) transparent constitué d'un premier canal (1) d'entrée. Dans le corps (24), une chambre (26) de concentration, comprenant une extrémité proximale raccordée au premier canal (1) d'entrée, s'étend le long d'un axe (28) longitudinal. Un premier canal (5) de sortie est raccordé à une extrémité distale de la chambre (26) de concentration. Un filtre (3), servant de paroi à la chambre (26) de concentration, est placé entre le premier canal (1) d'entrée et le premier canal (5) de sortie et s'étend dans un sens parallèle à l'axe (28) longitudinal.
CA002374649A 1999-03-22 2000-03-21 Appareil pour l'ecoulement tangentiel, la concentration et la fusion des cellules Abandoned CA2374649A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12558399P 1999-03-22 1999-03-22
US60/125,583 1999-03-22
PCT/US2000/006420 WO2000060065A1 (fr) 1999-03-22 2000-03-21 Appareil pour l'ecoulement tangentiel, la concentration et la fusion des cellules

Publications (1)

Publication Number Publication Date
CA2374649A1 true CA2374649A1 (fr) 2000-10-12

Family

ID=22420420

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002374649A Abandoned CA2374649A1 (fr) 1999-03-22 2000-03-21 Appareil pour l'ecoulement tangentiel, la concentration et la fusion des cellules

Country Status (3)

Country Link
EP (1) EP1137765A4 (fr)
CA (1) CA2374649A1 (fr)
WO (1) WO2000060065A1 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1458846A4 (fr) * 2001-12-06 2005-02-09 Bio Rad Laboratories Insert de cuvette d'electroporation facilitant la fusion par membrane
EP1620936A2 (fr) * 2003-03-17 2006-02-01 Virginia Tech Intellectual Properties, Inc. Appareil et methode permettant d'eviter un inversement d'ecoulement dans la matiere de retour arriere de stator d'un srm biphase (tpsrm)
AU2003902363A0 (en) * 2003-05-15 2003-05-29 Life Therapeutics Limited Cell separation
EP2270130B1 (fr) 2005-06-13 2020-04-29 Tosoh Corporation Dispositif pour la fusion des cellules et procédé d'utilisation de celui-ci

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2583769B1 (fr) * 1985-06-19 1987-09-18 Centre Nat Rech Scient Procede perfectionne de fusion cellulaire
US4832814A (en) * 1987-12-28 1989-05-23 E. I. Du Pont De Nemours And Company Electrofusion cell and method of making the same
US6010613A (en) * 1995-12-08 2000-01-04 Cyto Pulse Sciences, Inc. Method of treating materials with pulsed electrical fields

Also Published As

Publication number Publication date
EP1137765A4 (fr) 2002-09-18
WO2000060065A1 (fr) 2000-10-12
EP1137765A1 (fr) 2001-10-04

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Legal Events

Date Code Title Description
FZDE Discontinued