CA2374649A1 - Appareil pour l'ecoulement tangentiel, la concentration et la fusion des cellules - Google Patents
Appareil pour l'ecoulement tangentiel, la concentration et la fusion des cellules Download PDFInfo
- Publication number
- CA2374649A1 CA2374649A1 CA002374649A CA2374649A CA2374649A1 CA 2374649 A1 CA2374649 A1 CA 2374649A1 CA 002374649 A CA002374649 A CA 002374649A CA 2374649 A CA2374649 A CA 2374649A CA 2374649 A1 CA2374649 A1 CA 2374649A1
- Authority
- CA
- Canada
- Prior art keywords
- cell
- bladder
- cells
- fusion
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/18—Apparatus therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/20—Accessories; Auxiliary operations
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C5/00—Separating dispersed particles from liquids by electrostatic effect
- B03C5/02—Separators
- B03C5/022—Non-uniform field separators
- B03C5/026—Non-uniform field separators using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
Landscapes
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Water Supply & Treatment (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microelectronics & Electronic Packaging (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Electromagnetism (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
L'invention porte sur un appareil (12) utilisé pour l'écoulement tangentiel, la concentration et la fusion des cellules et comprenant un corps (24) transparent constitué d'un premier canal (1) d'entrée. Dans le corps (24), une chambre (26) de concentration, comprenant une extrémité proximale raccordée au premier canal (1) d'entrée, s'étend le long d'un axe (28) longitudinal. Un premier canal (5) de sortie est raccordé à une extrémité distale de la chambre (26) de concentration. Un filtre (3), servant de paroi à la chambre (26) de concentration, est placé entre le premier canal (1) d'entrée et le premier canal (5) de sortie et s'étend dans un sens parallèle à l'axe (28) longitudinal.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US12558399P | 1999-03-22 | 1999-03-22 | |
US60/125,583 | 1999-03-22 | ||
PCT/US2000/006420 WO2000060065A1 (fr) | 1999-03-22 | 2000-03-21 | Appareil pour l'ecoulement tangentiel, la concentration et la fusion des cellules |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2374649A1 true CA2374649A1 (fr) | 2000-10-12 |
Family
ID=22420420
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002374649A Abandoned CA2374649A1 (fr) | 1999-03-22 | 2000-03-21 | Appareil pour l'ecoulement tangentiel, la concentration et la fusion des cellules |
Country Status (3)
Country | Link |
---|---|
EP (1) | EP1137765A4 (fr) |
CA (1) | CA2374649A1 (fr) |
WO (1) | WO2000060065A1 (fr) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP4567337B2 (ja) * | 2001-12-06 | 2010-10-20 | バイオ−ラッド ラボラトリーズ,インコーポレイティド | メンブレン式融合を促進する電気穿孔キュベット用インサート |
US7015615B2 (en) * | 2003-03-17 | 2006-03-21 | Virginia Tech Intellectual Properties, Inc. | Apparatus and method that prevent flux reversal in the stator back material of a two-phase SRM (TPSRM) |
AU2003902363A0 (en) | 2003-05-15 | 2003-05-29 | Life Therapeutics Limited | Cell separation |
US20060281168A1 (en) * | 2005-06-13 | 2006-12-14 | Tosoh Corporation | Cell fusion chamber, cell fusion device, and method for cell fusion using the same |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2583769B1 (fr) * | 1985-06-19 | 1987-09-18 | Centre Nat Rech Scient | Procede perfectionne de fusion cellulaire |
US4832814A (en) * | 1987-12-28 | 1989-05-23 | E. I. Du Pont De Nemours And Company | Electrofusion cell and method of making the same |
US6010613A (en) * | 1995-12-08 | 2000-01-04 | Cyto Pulse Sciences, Inc. | Method of treating materials with pulsed electrical fields |
-
2000
- 2000-03-21 CA CA002374649A patent/CA2374649A1/fr not_active Abandoned
- 2000-03-21 EP EP00919388A patent/EP1137765A4/fr not_active Withdrawn
- 2000-03-21 WO PCT/US2000/006420 patent/WO2000060065A1/fr not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
EP1137765A1 (fr) | 2001-10-04 |
EP1137765A4 (fr) | 2002-09-18 |
WO2000060065A1 (fr) | 2000-10-12 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FZDE | Discontinued |