CA2373873A1 - Competitive chemiluminescent assay for cyclic nucleotide monophosphates - Google Patents
Competitive chemiluminescent assay for cyclic nucleotide monophosphates Download PDFInfo
- Publication number
- CA2373873A1 CA2373873A1 CA002373873A CA2373873A CA2373873A1 CA 2373873 A1 CA2373873 A1 CA 2373873A1 CA 002373873 A CA002373873 A CA 002373873A CA 2373873 A CA2373873 A CA 2373873A CA 2373873 A1 CA2373873 A1 CA 2373873A1
- Authority
- CA
- Canada
- Prior art keywords
- cyclic nucleotide
- kit
- camp
- cyclic
- antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000003556 assay Methods 0.000 title claims description 26
- -1 cyclic nucleotide monophosphates Chemical class 0.000 title description 10
- 230000002860 competitive effect Effects 0.000 title description 7
- BVTJGGGYKAMDBN-UHFFFAOYSA-N Dioxetane Chemical compound C1COO1 BVTJGGGYKAMDBN-UHFFFAOYSA-N 0.000 claims abstract description 29
- 125000004122 cyclic group Chemical group 0.000 claims abstract description 27
- 238000006243 chemical reaction Methods 0.000 claims abstract description 17
- 102000002260 Alkaline Phosphatase Human genes 0.000 claims abstract description 16
- 108020004774 Alkaline Phosphatase Proteins 0.000 claims abstract description 16
- 238000005406 washing Methods 0.000 claims abstract description 7
- IVOMOUWHDPKRLL-KQYNXXCUSA-N Cyclic adenosine monophosphate Chemical compound C([C@H]1O2)OP(O)(=O)O[C@H]1[C@@H](O)[C@@H]2N1C(N=CN=C2N)=C2N=C1 IVOMOUWHDPKRLL-KQYNXXCUSA-N 0.000 claims abstract description 6
- 238000000354 decomposition reaction Methods 0.000 claims abstract description 6
- 150000003839 salts Chemical class 0.000 claims abstract description 6
- 238000012875 competitive assay Methods 0.000 claims abstract description 3
- 238000000034 method Methods 0.000 claims description 13
- 229910019142 PO4 Inorganic materials 0.000 claims description 12
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 11
- 239000010452 phosphate Substances 0.000 claims description 11
- 239000011541 reaction mixture Substances 0.000 claims description 9
- 239000000872 buffer Substances 0.000 claims description 8
- 239000003153 chemical reaction reagent Substances 0.000 claims description 6
- 238000011534 incubation Methods 0.000 claims description 6
- 239000003623 enhancer Substances 0.000 claims description 5
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 claims description 3
- 239000008896 Opium Substances 0.000 claims description 3
- 229920001213 Polysorbate 20 Polymers 0.000 claims description 3
- 230000027455 binding Effects 0.000 claims description 3
- HFNQLYDPNAZRCH-UHFFFAOYSA-N carbonic acid Chemical compound OC(O)=O.OC(O)=O HFNQLYDPNAZRCH-UHFFFAOYSA-N 0.000 claims description 3
- 238000002967 competitive immunoassay Methods 0.000 claims description 3
- 229960001027 opium Drugs 0.000 claims description 3
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims description 3
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims description 3
- DJJCXFVJDGTHFX-UHFFFAOYSA-N Uridinemonophosphate Natural products OC1C(O)C(COP(O)(O)=O)OC1N1C(=O)NC(=O)C=C1 DJJCXFVJDGTHFX-UHFFFAOYSA-N 0.000 claims description 2
- IERHLVCPSMICTF-XVFCMESISA-N cytidine 5'-monophosphate Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(O)=O)O1 IERHLVCPSMICTF-XVFCMESISA-N 0.000 claims description 2
- IERHLVCPSMICTF-UHFFFAOYSA-N cytidine monophosphate Natural products O=C1N=C(N)C=CN1C1C(O)C(O)C(COP(O)(O)=O)O1 IERHLVCPSMICTF-UHFFFAOYSA-N 0.000 claims description 2
- 239000000376 reactant Substances 0.000 claims description 2
- DJJCXFVJDGTHFX-XVFCMESISA-N uridine 5'-monophosphate Chemical compound O[C@@H]1[C@H](O)[C@@H](COP(O)(O)=O)O[C@H]1N1C(=O)NC(=O)C=C1 DJJCXFVJDGTHFX-XVFCMESISA-N 0.000 claims description 2
- ZOOGRGPOEVQQDX-KHLHZJAASA-N cyclic guanosine monophosphate Chemical compound C([C@H]1O2)O[P@](O)(=O)O[C@@H]1[C@H](O)[C@H]2N1C(N=C(NC2=O)N)=C2N=C1 ZOOGRGPOEVQQDX-KHLHZJAASA-N 0.000 claims 3
- 230000001960 triggered effect Effects 0.000 claims 3
- 239000003795 chemical substances by application Substances 0.000 abstract description 8
- 239000000203 mixture Substances 0.000 abstract description 2
- 230000035945 sensitivity Effects 0.000 description 13
- 238000001514 detection method Methods 0.000 description 6
- 125000001424 substituent group Chemical group 0.000 description 6
- 229910052739 hydrogen Inorganic materials 0.000 description 4
- 239000001257 hydrogen Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 230000004044 response Effects 0.000 description 4
- ZOOGRGPOEVQQDX-UUOKFMHZSA-N 3',5'-cyclic GMP Chemical compound C([C@H]1O2)OP(O)(=O)O[C@H]1[C@@H](O)[C@@H]2N1C(N=C(NC2=O)N)=C2N=C1 ZOOGRGPOEVQQDX-UUOKFMHZSA-N 0.000 description 3
- 238000012286 ELISA Assay Methods 0.000 description 3
- QWXOJIDBSHLIFI-UHFFFAOYSA-N [3-(1-chloro-3'-methoxyspiro[adamantane-4,4'-dioxetane]-3'-yl)phenyl] dihydrogen phosphate Chemical compound O1OC2(C3CC4CC2CC(Cl)(C4)C3)C1(OC)C1=CC=CC(OP(O)(O)=O)=C1 QWXOJIDBSHLIFI-UHFFFAOYSA-N 0.000 description 3
- 230000021736 acetylation Effects 0.000 description 3
- 238000006640 acetylation reaction Methods 0.000 description 3
- 125000000217 alkyl group Chemical group 0.000 description 3
- 229910052801 chlorine Inorganic materials 0.000 description 3
- 230000002503 metabolic effect Effects 0.000 description 3
- 150000004712 monophosphates Chemical class 0.000 description 3
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- 239000011534 wash buffer Substances 0.000 description 3
- NMPZCCZXCOMSDQ-XVFCMESISA-N 2',3'-cyclic CMP Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@@H]2OP(O)(=O)O[C@@H]2[C@@H](CO)O1 NMPZCCZXCOMSDQ-XVFCMESISA-N 0.000 description 2
- HWDMHJDYMFRXOX-XVFCMESISA-N 2',3'-cyclic UMP Chemical compound N1([C@@H]2O[C@@H]([C@H]3OP(O)(=O)O[C@H]32)CO)C=CC(=O)NC1=O HWDMHJDYMFRXOX-XVFCMESISA-N 0.000 description 2
- HWDMHJDYMFRXOX-UHFFFAOYSA-N CUMP Natural products C12OP(O)(=O)OC2C(CO)OC1N1C=CC(=O)NC1=O HWDMHJDYMFRXOX-UHFFFAOYSA-N 0.000 description 2
- 241000283707 Capra Species 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 229920002527 Glycogen Polymers 0.000 description 2
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 2
- 125000005073 adamantyl group Chemical group C12(CC3CC(CC(C1)C3)C2)* 0.000 description 2
- 230000001464 adherent effect Effects 0.000 description 2
- 125000003118 aryl group Chemical group 0.000 description 2
- 239000012131 assay buffer Substances 0.000 description 2
- 238000003149 assay kit Methods 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- WCPTXJJVVDAEMW-UHFFFAOYSA-N cCMP Natural products O=C1N=C(N)C=CN1C1C(O)C2OP(O)(=O)OCC2O1 WCPTXJJVVDAEMW-UHFFFAOYSA-N 0.000 description 2
- 125000001309 chloro group Chemical group Cl* 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 229940096919 glycogen Drugs 0.000 description 2
- 150000002367 halogens Chemical class 0.000 description 2
- 230000003054 hormonal effect Effects 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 150000002431 hydrogen Chemical class 0.000 description 2
- 230000002209 hydrophobic effect Effects 0.000 description 2
- 238000003018 immunoassay Methods 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 235000021317 phosphate Nutrition 0.000 description 2
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- UCTWMZQNUQWSLP-VIFPVBQESA-N (R)-adrenaline Chemical compound CNC[C@H](O)C1=CC=C(O)C(O)=C1 UCTWMZQNUQWSLP-VIFPVBQESA-N 0.000 description 1
- 229930182837 (R)-adrenaline Natural products 0.000 description 1
- DMJWGQPYNRPLGA-KQYNXXCUSA-N 3',5'-cyclic IMP Chemical compound C1=NC2=C(O)N=CN=C2N1[C@@H]1O[C@@H]2COP(O)(=O)O[C@H]2[C@H]1O DMJWGQPYNRPLGA-KQYNXXCUSA-N 0.000 description 1
- QSJFDOVQWZVUQG-XLPZGREQSA-N 3',5'-cyclic dTMP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@@H]2COP(O)(=O)O[C@H]2C1 QSJFDOVQWZVUQG-XLPZGREQSA-N 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 239000000055 Corticotropin-Releasing Hormone Substances 0.000 description 1
- 102000008130 Cyclic AMP-Dependent Protein Kinases Human genes 0.000 description 1
- 108010049894 Cyclic AMP-Dependent Protein Kinases Proteins 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- GMPKIPWJBDOURN-UHFFFAOYSA-N Methoxyamine Chemical compound CON GMPKIPWJBDOURN-UHFFFAOYSA-N 0.000 description 1
- 101100206458 Mus musculus Them4 gene Proteins 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 102000003982 Parathyroid hormone Human genes 0.000 description 1
- 108090000445 Parathyroid hormone Proteins 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- IVOMOUWHDPKRLL-UHFFFAOYSA-N UNPD107823 Natural products O1C2COP(O)(=O)OC2C(O)C1N1C(N=CN=C2N)=C2N=C1 IVOMOUWHDPKRLL-UHFFFAOYSA-N 0.000 description 1
- GXBMIBRIOWHPDT-UHFFFAOYSA-N Vasopressin Natural products N1C(=O)C(CC=2C=C(O)C=CC=2)NC(=O)C(N)CSSCC(C(=O)N2C(CCC2)C(=O)NC(CCCN=C(N)N)C(=O)NCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(CCC(N)=O)NC(=O)C1CC1=CC=CC=C1 GXBMIBRIOWHPDT-UHFFFAOYSA-N 0.000 description 1
- 102000002852 Vasopressins Human genes 0.000 description 1
- 108010004977 Vasopressins Proteins 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 125000003545 alkoxy group Chemical group 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- KBZOIRJILGZLEJ-LGYYRGKSSA-N argipressin Chemical compound C([C@H]1C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CSSC[C@@H](C(N[C@@H](CC=2C=CC(O)=CC=2)C(=O)N1)=O)N)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCN=C(N)N)C(=O)NCC(N)=O)C1=CC=CC=C1 KBZOIRJILGZLEJ-LGYYRGKSSA-N 0.000 description 1
- 125000003710 aryl alkyl group Chemical group 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- UASRYODFRYWBRC-UHFFFAOYSA-N cGMP Natural products N1C(N)=NC(=O)C2=C1N(C1C3OP(O)(=O)OC3C(CO)O1)C=N2 UASRYODFRYWBRC-UHFFFAOYSA-N 0.000 description 1
- DMJWGQPYNRPLGA-UHFFFAOYSA-N cIMP Natural products C1=NC(C(N=CN2)=O)=C2N1C1C(O)C2OP(O)(=O)OCC2O1 DMJWGQPYNRPLGA-UHFFFAOYSA-N 0.000 description 1
- 125000004432 carbon atom Chemical group C* 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 230000004640 cellular pathway Effects 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- IDLFZVILOHSSID-OVLDLUHVSA-N corticotropin Chemical compound C([C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(N)=O)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(O)=O)NC(=O)[C@@H](N)CO)C1=CC=C(O)C=C1 IDLFZVILOHSSID-OVLDLUHVSA-N 0.000 description 1
- 229960000258 corticotropin Drugs 0.000 description 1
- ZOOGRGPOEVQQDX-UHFFFAOYSA-N cyclic GMP Natural products O1C2COP(O)(=O)OC2C(O)C1N1C=NC2=C1NC(N)=NC2=O ZOOGRGPOEVQQDX-UHFFFAOYSA-N 0.000 description 1
- 229940095074 cyclic amp Drugs 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 235000019800 disodium phosphate Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229960005139 epinephrine Drugs 0.000 description 1
- 230000007185 extracellular pathway Effects 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 125000001153 fluoro group Chemical group F* 0.000 description 1
- RQFCJASXJCIDSX-UUOKFMHZSA-N guanosine 5'-monophosphate Chemical compound C1=2NC(N)=NC(=O)C=2N=CN1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O RQFCJASXJCIDSX-UUOKFMHZSA-N 0.000 description 1
- 235000013928 guanylic acid Nutrition 0.000 description 1
- 229910052736 halogen Inorganic materials 0.000 description 1
- 125000004404 heteroalkyl group Chemical group 0.000 description 1
- 230000002163 immunogen Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000008611 intercellular interaction Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 230000006662 intracellular pathway Effects 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000002934 lysing effect Effects 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 125000001624 naphthyl group Chemical group 0.000 description 1
- 230000009871 nonspecific binding Effects 0.000 description 1
- 239000000199 parathyroid hormone Substances 0.000 description 1
- 229960001319 parathyroid hormone Drugs 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- XYFCBTPGUUZFHI-UHFFFAOYSA-O phosphonium Chemical compound [PH4+] XYFCBTPGUUZFHI-UHFFFAOYSA-O 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 150000003180 prostaglandins Chemical class 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000003127 radioimmunoassay Methods 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-O sulfonium Chemical compound [SH3+] RWSOTUBLDIXVET-UHFFFAOYSA-O 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 229960003726 vasopressin Drugs 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Pathology (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Zoology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/307,797 US6686171B2 (en) | 1999-05-10 | 1999-05-10 | Competitive chemiluminescent assay for cyclic nucleotide monophosphates |
| US09/307,797 | 1999-05-10 | ||
| PCT/US2000/011726 WO2000067804A1 (en) | 1999-05-10 | 2000-05-02 | Competitive chemiluminescent assay for cyclic nucleotide monophosphates |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2373873A1 true CA2373873A1 (en) | 2000-11-16 |
Family
ID=23191203
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002373873A Abandoned CA2373873A1 (en) | 1999-05-10 | 2000-05-02 | Competitive chemiluminescent assay for cyclic nucleotide monophosphates |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US6686171B2 (enExample) |
| EP (2) | EP1181059B1 (enExample) |
| JP (1) | JP5021120B2 (enExample) |
| AT (1) | ATE477001T1 (enExample) |
| AU (1) | AU4811400A (enExample) |
| CA (1) | CA2373873A1 (enExample) |
| DE (1) | DE60044809D1 (enExample) |
| WO (1) | WO2000067804A1 (enExample) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6794156B2 (en) * | 1999-05-10 | 2004-09-21 | Applera Corporation | Cell growth, induction and lysis in an antibody-coated microplate for use in an ELISA |
| US8168592B2 (en) * | 2005-10-21 | 2012-05-01 | Amgen Inc. | CGRP peptide antagonists and conjugates |
| ES2452318T3 (es) | 2006-12-21 | 2014-03-31 | F. Hoffmann-La Roche Ag | Método para la detección de AMPc y GMPc |
| US20130017197A1 (en) * | 2010-03-31 | 2013-01-17 | Universite De Geneve | Stabilized antibody preparations and uses thereof |
| US10767234B2 (en) | 2017-03-31 | 2020-09-08 | Regents Of The University Of Minnesota | Methods for microbial screening and identification of targets of interest |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5825980B2 (ja) * | 1976-02-12 | 1983-05-31 | ヤマサ醤油株式会社 | サイクリックヌクレオチドの定量法 |
| JPS57146594A (en) * | 1981-03-06 | 1982-09-10 | Yamasa Shoyu Co Ltd | Preparation of anticyclic nucleotide antibody |
| US4962192A (en) | 1986-07-17 | 1990-10-09 | Board Of Governors Of Wayne State University | Chemiluminescent 1,2-dioxetane compounds |
| CA1340590C (en) * | 1986-07-24 | 1999-06-08 | John C. Voyta | Chemiluminescence enhancement |
| US5112960A (en) | 1989-07-17 | 1992-05-12 | Bronstein Irena Y | Chemiluminescent 3-(substituted adamant-2'-ylidene) 1,2-dioxetanes |
| US5856522A (en) | 1995-05-04 | 1999-01-05 | Tropix, Inc. | Method of using synthesis of 1,2-dioxetanes and kits therefore |
| US5089424A (en) * | 1988-06-14 | 1992-02-18 | Abbott Laboratories | Method and apparatus for heterogeneous chemiluminescence assay |
| US4931569A (en) | 1988-09-14 | 1990-06-05 | Tropix, Inc. | Purification of stable water-soluble dioxetanes |
| JPH05504683A (ja) * | 1989-12-20 | 1993-07-22 | ニコルス インスティテュート ダイアグノスティックス | 組み換えチロトロピン受容体 |
| US5547836A (en) * | 1990-08-30 | 1996-08-20 | Tropix, Inc. | Enhancement of chemiluminescent assays |
| JP2999810B2 (ja) * | 1990-09-10 | 2000-01-17 | トロピックス・インコーポレーテッド | １，２−ジオキセタン化合物 |
| DE69210225T2 (de) * | 1991-02-14 | 1996-12-05 | Baxter Int | Herstellungsverfahren für biegsame biologische Gewebeverpflanzungsmaterialen |
| US5399500A (en) * | 1992-06-26 | 1995-03-21 | Becton Dickinson And Company | Two step process for coating of antibodies to a solid phase |
| DE69430322T2 (de) * | 1993-12-23 | 2002-11-14 | Tropix, Inc. | Chemilumineszenz-assay mittels energietransfer |
| US5589344A (en) * | 1994-06-15 | 1996-12-31 | Johnson & Johnson Clinical Diagnostics, Inc. | Test kit and method for competitive specific binding assay |
| US5739001A (en) * | 1996-10-29 | 1998-04-14 | E. I. Du Pont De Nemours And Company | Solid phase cell-based assay |
-
1999
- 1999-05-10 US US09/307,797 patent/US6686171B2/en not_active Expired - Lifetime
-
2000
- 2000-05-02 AU AU48114/00A patent/AU4811400A/en not_active Abandoned
- 2000-05-02 EP EP00930260A patent/EP1181059B1/en not_active Expired - Lifetime
- 2000-05-02 AT AT00930260T patent/ATE477001T1/de not_active IP Right Cessation
- 2000-05-02 EP EP20100158650 patent/EP2213311B1/en not_active Expired - Lifetime
- 2000-05-02 WO PCT/US2000/011726 patent/WO2000067804A1/en not_active Ceased
- 2000-05-02 CA CA002373873A patent/CA2373873A1/en not_active Abandoned
- 2000-05-02 JP JP2000616829A patent/JP5021120B2/ja not_active Expired - Lifetime
- 2000-05-02 DE DE60044809T patent/DE60044809D1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| AU4811400A (en) | 2000-11-21 |
| US20020115123A1 (en) | 2002-08-22 |
| US6686171B2 (en) | 2004-02-03 |
| EP1181059A1 (en) | 2002-02-27 |
| WO2000067804A1 (en) | 2000-11-16 |
| DE60044809D1 (de) | 2010-09-23 |
| EP2213311A1 (en) | 2010-08-04 |
| ATE477001T1 (de) | 2010-08-15 |
| EP2213311B1 (en) | 2015-04-22 |
| JP5021120B2 (ja) | 2012-09-05 |
| EP1181059A4 (en) | 2005-01-12 |
| JP2002544493A (ja) | 2002-12-24 |
| EP1181059B1 (en) | 2010-08-11 |
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| Date | Code | Title | Description |
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| FZDE | Discontinued |