CA2372411A1 - Procede pour separer et/ou isoler des proteines plasmatiques par chromatographie annulaire - Google Patents
Procede pour separer et/ou isoler des proteines plasmatiques par chromatographie annulaire Download PDFInfo
- Publication number
- CA2372411A1 CA2372411A1 CA002372411A CA2372411A CA2372411A1 CA 2372411 A1 CA2372411 A1 CA 2372411A1 CA 002372411 A CA002372411 A CA 002372411A CA 2372411 A CA2372411 A CA 2372411A CA 2372411 A1 CA2372411 A1 CA 2372411A1
- Authority
- CA
- Canada
- Prior art keywords
- separation
- process according
- plasma proteins
- separation medium
- annular design
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 102000004506 Blood Proteins Human genes 0.000 title claims abstract description 36
- 108010017384 Blood Proteins Proteins 0.000 title claims abstract description 36
- 238000000034 method Methods 0.000 title claims abstract description 32
- 238000004587 chromatography analysis Methods 0.000 title claims description 15
- 238000000926 separation method Methods 0.000 claims abstract description 61
- 239000000203 mixture Substances 0.000 claims abstract description 21
- 239000003480 eluent Substances 0.000 claims abstract description 11
- 238000002955 isolation Methods 0.000 claims abstract description 4
- 239000000463 material Substances 0.000 claims description 20
- 210000002381 plasma Anatomy 0.000 claims description 7
- 102000003886 Glycoproteins Human genes 0.000 claims description 5
- 108090000288 Glycoproteins Proteins 0.000 claims description 5
- 108060003951 Immunoglobulin Proteins 0.000 claims description 5
- 102000018358 immunoglobulin Human genes 0.000 claims description 5
- 230000003993 interaction Effects 0.000 claims description 5
- 230000001419 dependent effect Effects 0.000 claims description 4
- 238000001542 size-exclusion chromatography Methods 0.000 claims description 4
- 239000012798 spherical particle Substances 0.000 claims description 4
- 239000002753 trypsin inhibitor Substances 0.000 claims description 4
- 229930003448 Vitamin K Natural products 0.000 claims description 3
- 238000005377 adsorption chromatography Methods 0.000 claims description 3
- 238000001042 affinity chromatography Methods 0.000 claims description 3
- 239000003114 blood coagulation factor Substances 0.000 claims description 3
- SHUZOJHMOBOZST-UHFFFAOYSA-N phylloquinone Natural products CC(C)CCCCC(C)CCC(C)CCCC(=CCC1=C(C)C(=O)c2ccccc2C1=O)C SHUZOJHMOBOZST-UHFFFAOYSA-N 0.000 claims description 3
- 235000019168 vitamin K Nutrition 0.000 claims description 3
- 239000011712 vitamin K Substances 0.000 claims description 3
- 150000003721 vitamin K derivatives Chemical class 0.000 claims description 3
- 229940046010 vitamin k Drugs 0.000 claims description 3
- 108090000935 Antithrombin III Proteins 0.000 claims description 2
- 102000004411 Antithrombin III Human genes 0.000 claims description 2
- 206010053567 Coagulopathies Diseases 0.000 claims description 2
- 108091006905 Human Serum Albumin Proteins 0.000 claims description 2
- 102000008100 Human Serum Albumin Human genes 0.000 claims description 2
- 108090000190 Thrombin Proteins 0.000 claims description 2
- 229940122618 Trypsin inhibitor Drugs 0.000 claims description 2
- 241000700605 Viruses Species 0.000 claims description 2
- 229960005348 antithrombin iii Drugs 0.000 claims description 2
- 230000035602 clotting Effects 0.000 claims description 2
- 230000005661 hydrophobic surface Effects 0.000 claims description 2
- 238000005342 ion exchange Methods 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 229960004072 thrombin Drugs 0.000 claims description 2
- 239000000523 sample Substances 0.000 description 12
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 7
- 229940098773 bovine serum albumin Drugs 0.000 description 7
- 235000018102 proteins Nutrition 0.000 description 7
- 102000004169 proteins and genes Human genes 0.000 description 7
- 108090000623 proteins and genes Proteins 0.000 description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 108010054218 Factor VIII Proteins 0.000 description 5
- 102000001690 Factor VIII Human genes 0.000 description 5
- 239000000872 buffer Substances 0.000 description 5
- 229960000301 factor viii Drugs 0.000 description 5
- 239000002245 particle Substances 0.000 description 5
- 102100022641 Coagulation factor IX Human genes 0.000 description 4
- 108010076282 Factor IX Proteins 0.000 description 4
- 102000003839 Human Proteins Human genes 0.000 description 4
- 108090000144 Human Proteins Proteins 0.000 description 4
- 229960004222 factor ix Drugs 0.000 description 4
- 230000002209 hydrophobic effect Effects 0.000 description 4
- 238000011084 recovery Methods 0.000 description 4
- 239000007858 starting material Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 238000010828 elution Methods 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- MYRTYDVEIRVNKP-UHFFFAOYSA-N 1,2-Divinylbenzene Chemical compound C=CC1=CC=CC=C1C=C MYRTYDVEIRVNKP-UHFFFAOYSA-N 0.000 description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
- 150000001450 anions Chemical class 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 230000023555 blood coagulation Effects 0.000 description 2
- 239000001110 calcium chloride Substances 0.000 description 2
- 229910001628 calcium chloride Inorganic materials 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 239000001509 sodium citrate Substances 0.000 description 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 1
- 108010049003 Fibrinogen Proteins 0.000 description 1
- 102000008946 Fibrinogen Human genes 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- 206010022528 Interactions Diseases 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 238000005349 anion exchange Methods 0.000 description 1
- 238000005571 anion exchange chromatography Methods 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 238000011033 desalting Methods 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 229940076247 disodium hydrogen phosphate dehydrate Drugs 0.000 description 1
- 229940116441 divinylbenzene Drugs 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 229940003169 factor viii / von willebrand factor Drugs 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 229940012952 fibrinogen Drugs 0.000 description 1
- 238000005227 gel permeation chromatography Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 238000004191 hydrophobic interaction chromatography Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 229910000403 monosodium phosphate Inorganic materials 0.000 description 1
- 235000019799 monosodium phosphate Nutrition 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 238000005498 polishing Methods 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 229920000193 polymethacrylate Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 108010012557 prothrombin complex concentrates Proteins 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 238000002444 silanisation Methods 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 108010047303 von Willebrand Factor Proteins 0.000 description 1
- 102100036537 von Willebrand factor Human genes 0.000 description 1
- 229960001134 von willebrand factor Drugs 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Landscapes
- Peptides Or Proteins (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19823814.2 | 1998-05-27 | ||
| DE19823814A DE19823814A1 (de) | 1998-05-27 | 1998-05-27 | Verfahren zur Trennung und/oder Isolierung von Plasmaproteinen mit annularer Chromatographie |
| CA002333316A CA2333316A1 (fr) | 1998-05-27 | 1999-05-27 | Procede pour separer et/ou isoler des proteines plasmatiques par chromatographie annulaire |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002333316A Division CA2333316A1 (fr) | 1998-05-27 | 1999-05-27 | Procede pour separer et/ou isoler des proteines plasmatiques par chromatographie annulaire |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2372411A1 true CA2372411A1 (fr) | 1999-12-02 |
Family
ID=25682366
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002372411A Abandoned CA2372411A1 (fr) | 1998-05-27 | 1999-05-27 | Procede pour separer et/ou isoler des proteines plasmatiques par chromatographie annulaire |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2372411A1 (fr) |
-
1999
- 1999-05-27 CA CA002372411A patent/CA2372411A1/fr not_active Abandoned
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Dead |