CA2371727A1 - Analyse electrophoretique de molecules cibles au moyen de molecules adaptateurs - Google Patents

Analyse electrophoretique de molecules cibles au moyen de molecules adaptateurs Download PDF

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Publication number
CA2371727A1
CA2371727A1 CA002371727A CA2371727A CA2371727A1 CA 2371727 A1 CA2371727 A1 CA 2371727A1 CA 002371727 A CA002371727 A CA 002371727A CA 2371727 A CA2371727 A CA 2371727A CA 2371727 A1 CA2371727 A1 CA 2371727A1
Authority
CA
Canada
Prior art keywords
adapter
target
molecule
capture probe
nucleotide sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002371727A
Other languages
English (en)
Inventor
Lawrence Weir
T. Christian Boles
Ezra S. Abrams
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Exact Sciences Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2371727A1 publication Critical patent/CA2371727A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Abstract

L'invention concerne un gel universel et des procédés d'utilisation destinés à détecter la présence ou l'absence d'au moins une molécule cible dans un échantillon d'essai, consistant à former un complexe d'hybridation de gel universel dans lequel une molécule adaptateur est hybridée à une molécule cible et à une sonde de capture universelle.
CA002371727A 1999-04-02 2000-03-31 Analyse electrophoretique de molecules cibles au moyen de molecules adaptateurs Abandoned CA2371727A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US28538099A 1999-04-02 1999-04-02
US09/285,380 1999-04-02
PCT/US2000/008529 WO2000060118A2 (fr) 1999-04-02 2000-03-31 Analyse electrophoretique de molecules cibles au moyen de molecules adaptateurs

Publications (1)

Publication Number Publication Date
CA2371727A1 true CA2371727A1 (fr) 2000-10-12

Family

ID=23093992

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002371727A Abandoned CA2371727A1 (fr) 1999-04-02 2000-03-31 Analyse electrophoretique de molecules cibles au moyen de molecules adaptateurs

Country Status (5)

Country Link
EP (1) EP1235930A2 (fr)
JP (1) JP2003508015A (fr)
AU (1) AU4053900A (fr)
CA (1) CA2371727A1 (fr)
WO (1) WO2000060118A2 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4276301B2 (ja) 1997-05-16 2009-06-10 エグザクト サイエンシーズ コーポレイション 固定化プローブを用いる、分子の電気泳動分析
JP2003503011A (ja) * 1999-04-02 2003-01-28 エムティー テクノロジー，インコーポレイテッド 固定プローブを使用する微生物の検出方法
US6586177B1 (en) 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
US7993853B2 (en) * 2005-05-06 2011-08-09 Gen-Probe Incorporated Methods of nucleic acid target capture
JP5201573B2 (ja) * 2006-01-24 2013-06-05 株式会社テクノメディカ 試料中の被検物質の測定方法及び装置
JP2010518398A (ja) * 2007-02-07 2010-05-27 ライザー ダイアグノスティックス， インコーポレイテッド 電気泳動を使用する迅速な均一系イムノアッセイ
US9976984B2 (en) * 2013-05-10 2018-05-22 The Regents Of The University Of California Free-standing microfluidic gel electrophoresis devices and methods

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0347097A (ja) * 1989-07-13 1991-02-28 Hitachi Ltd ハイブリダイゼーション方法、これを用いた遺伝子変異検出方法及びその装置
US5445933A (en) * 1992-09-15 1995-08-29 Boehringer Mannheim Corporation Strand displacement assay and complex useful therefor
EP2573101B1 (fr) * 1996-02-09 2016-04-20 Cornell Research Foundation, Inc. Détection de différences entre séquences d'acide nucléique faisant appel à la réaction de détection de ligase et à des réseaux adressables
US5932711A (en) * 1997-03-05 1999-08-03 Mosaic Technologies, Inc. Nucleic acid-containing polymerizable complex
JP4276301B2 (ja) * 1997-05-16 2009-06-10 エグザクト サイエンシーズ コーポレイション 固定化プローブを用いる、分子の電気泳動分析
JP2002518026A (ja) * 1998-06-19 2002-06-25 エムティー テクノロジー， インコーポレイテッド Ｓｒｐｒｎａを用いる非ウイルス生物の検出

Also Published As

Publication number Publication date
EP1235930A2 (fr) 2002-09-04
JP2003508015A (ja) 2003-03-04
WO2000060118A3 (fr) 2002-07-11
WO2000060118A2 (fr) 2000-10-12
AU4053900A (en) 2000-10-23

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