CA2367723A1 - Procedes d'obtention de sequences d'acide nucleique pleine longueur a l'aide de e. coli topoisomerase iii et ses homologues - Google Patents

Procedes d'obtention de sequences d'acide nucleique pleine longueur a l'aide de e. coli topoisomerase iii et ses homologues Download PDF

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Publication number
CA2367723A1
CA2367723A1 CA002367723A CA2367723A CA2367723A1 CA 2367723 A1 CA2367723 A1 CA 2367723A1 CA 002367723 A CA002367723 A CA 002367723A CA 2367723 A CA2367723 A CA 2367723A CA 2367723 A1 CA2367723 A1 CA 2367723A1
Authority
CA
Canada
Prior art keywords
mrna
cdna
expression vector
sequence
tag
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002367723A
Other languages
English (en)
Inventor
John A. Heyman
John D. Comiskey
Robert Marcil
Dorothy Phelan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2367723A1 publication Critical patent/CA2367723A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C07K14/4705—Regulators; Modulating activity stimulating, promoting or activating activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Abstract

L'invention porte sur un procédé d'obtention d'un ADNc codant pleine longueur. Ce procédé consiste à isoler un ARNm pleine longueur afin de synthétiser un premier brin d'ADNc, à fixer une étiquette non indigène sur l'ADNc et à utiliser le premier brin étiqueté pour synthétiser le second brin d'ADNc. L'ADNc final peut être ensuite amplifié et inséré dans un vecteur d'expression. L'invention porte également sur des ADNc codant pleine longueur préparés selon ce procédé, ainsi que sur des produits de recombinaison d'acide nucléique contenant les ADNc codant pleine longueur.
CA002367723A 1999-03-19 2000-03-13 Procedes d'obtention de sequences d'acide nucleique pleine longueur a l'aide de e. coli topoisomerase iii et ses homologues Abandoned CA2367723A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12512699P 1999-03-19 1999-03-19
US60/125,126 1999-03-19
PCT/US2000/006560 WO2000056878A1 (fr) 1999-03-19 2000-03-13 Procedes d'obtention de sequences d'acide nucleique pleine longueur a l'aide de e. coli topoisomerase iii et ses homologues

Publications (1)

Publication Number Publication Date
CA2367723A1 true CA2367723A1 (fr) 2000-09-28

Family

ID=22418306

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002367723A Abandoned CA2367723A1 (fr) 1999-03-19 2000-03-13 Procedes d'obtention de sequences d'acide nucleique pleine longueur a l'aide de e. coli topoisomerase iii et ses homologues

Country Status (5)

Country Link
EP (1) EP1165768A1 (fr)
JP (1) JP2002539784A (fr)
AU (1) AU3397800A (fr)
CA (1) CA2367723A1 (fr)
WO (1) WO2000056878A1 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5766891A (en) 1994-12-19 1998-06-16 Sloan-Kettering Institute For Cancer Research Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase
JP4447664B2 (ja) 1997-06-12 2010-04-07 スローン―ケタリング・インスティテュート・フォー・キャンサー・リサーチ ワクシニアトポイソメラーゼにより触媒されるｒｎａ鎖に対するｄｎａ鎖の共有結合
US6326175B1 (en) * 1999-07-13 2001-12-04 Incyte Pharmaceuticals, Inc. Methods and compositions for producing full length cDNA libraries
JP2003524422A (ja) 2000-02-25 2003-08-19 インヴィトロジェン コーポレーション トポイソメラーゼリンカー介在型増幅法
ATE315086T1 (de) 2000-08-21 2006-02-15 Invitrogen Corp Methoden und reagenzien für molekulares klonieren
US7033801B2 (en) 2000-12-08 2006-04-25 Invitrogen Corporation Compositions and methods for rapidly generating recombinant nucleic acid molecules
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US20040166499A1 (en) 2000-10-05 2004-08-26 Yoshihide Hayashizaki Oligonucleotide linkers comprising a variable cohesive portion and method for the praparation of polynucleotide libraries by using said linkers
US20060008817A1 (en) 2000-12-08 2006-01-12 Invitrogen Corporation Methods and compositions for generating recombinant nucleic acid molecules
EP1697534B1 (fr) 2003-12-01 2010-06-02 Life Technologies Corporation Molecule d'acide nucleique contenant des sites de recombinaison et leurs procedes d'utilisation

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2005252C (fr) * 1988-12-13 2001-07-17 Nahum Sonenberg Isolement de clones d'adn-c pleine longueur et de mrna chapeautes
JP3337748B2 (ja) * 1992-09-25 2002-10-21 財団法人神奈川科学技術アカデミー 完全長ｃＤＮＡの合成方法、その中間体の製造方法及び完全長ｃＤＮＡを含む組換えベクターの製造方法
US5766891A (en) * 1994-12-19 1998-06-16 Sloan-Kettering Institute For Cancer Research Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase
FR2733762B1 (fr) * 1995-05-02 1997-08-01 Genset Sa Methode de couplage specifique de la coiffe de l'extremite 5' d'un fragment d'arnm et preparation d'arnm et d'adnc complet
US5962271A (en) * 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
JP3441899B2 (ja) * 1996-11-01 2003-09-02 理化学研究所 完全長ｃＤＮＡライブラリーの作成方法
WO1998055502A1 (fr) * 1997-06-04 1998-12-10 Smithkline Beecham Corporation METHODES DE CLONAGE RAPIDE D'ADNc DE LONGUEUR TOTALE
JP4447664B2 (ja) * 1997-06-12 2010-04-07 スローン―ケタリング・インスティテュート・フォー・キャンサー・リサーチ ワクシニアトポイソメラーゼにより触媒されるｒｎａ鎖に対するｄｎａ鎖の共有結合

Also Published As

Publication number Publication date
JP2002539784A (ja) 2002-11-26
AU3397800A (en) 2000-10-09
WO2000056878A1 (fr) 2000-09-28
EP1165768A1 (fr) 2002-01-02

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Legal Events

Date Code Title Description
FZDE Discontinued