CA2358548A1 - Co-expression des proteines - Google Patents

Co-expression des proteines Download PDF

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Publication number
CA2358548A1
CA2358548A1 CA002358548A CA2358548A CA2358548A1 CA 2358548 A1 CA2358548 A1 CA 2358548A1 CA 002358548 A CA002358548 A CA 002358548A CA 2358548 A CA2358548 A CA 2358548A CA 2358548 A1 CA2358548 A1 CA 2358548A1
Authority
CA
Canada
Prior art keywords
protein
zero
plant
proteins
okd
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002358548A
Other languages
English (en)
Inventor
Suman Bagga
Champa Sengupta-Gopalan
John D. Kemp
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Arrowhead Center Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2358548A1 publication Critical patent/CA2358548A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254—Tryptophan or lysine
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C07K14/425—Zeins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8253—Methionine or cysteine

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Fodder In General (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne des matériaux et des procédés pour des plantes transformées et pour des tissus de plantes qui permettent d'exprimer de fortes teneurs en protéines stables qui sont localisées sous forme de corps protéiques dans la cellule de la plante. L'invention concerne des plantes transformées co-exprimant des fortes concentrations de protéines de zéine 15kD et 10 kD qui s'accumulent sous forme de taux élevés de corps protéiques dans le tissu végétatif de la plante. Les plantes transformées co-exprimant les protéines de zéine 15kD et 10kD permettent de constituer des cultures fourragères contenant des taux accrûs d'acides aminés à base de soufre, comme la méthionine, dans l'alimentation des animaux nourris par ces cultures. L'invention concerne également des plantes ou des tissus de plantes transformés comprenant des corps protéiques stables qui renferment un matériau protéinique hétérologue. Selon un mode de réalisation, un corps protéique stable est exprimé dans une plante ou un tissu de plante sous forme d'une protéine de fusion comprenant une protéine de zéine et une protéine ou un peptide à liaison opérationnelle. Les corps protéiques selon l'invention résiste à la dégradation par l'environnement ou par la digestion du ruminant.
CA002358548A 1998-12-31 1999-04-16 Co-expression des proteines Abandoned CA2358548A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US22465598A 1998-12-31 1998-12-31
US09/224,655 1998-12-31
PCT/US1999/008525 WO2000040738A1 (fr) 1998-12-31 1999-04-16 Co-expression des proteines

Publications (1)

Publication Number Publication Date
CA2358548A1 true CA2358548A1 (fr) 2000-07-13

Family

ID=22841589

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002358548A Abandoned CA2358548A1 (fr) 1998-12-31 1999-04-16 Co-expression des proteines

Country Status (8)

Country Link
EP (1) EP1141347A1 (fr)
JP (1) JP2002541768A (fr)
CN (1) CN1334877A (fr)
AR (1) AR016256A1 (fr)
AU (1) AU3652299A (fr)
BR (1) BR9916731A (fr)
CA (1) CA2358548A1 (fr)
WO (1) WO2000040738A1 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2224792B1 (es) * 2002-06-28 2007-02-16 Era Plantech, S.L. Produccion de peptidos y proteinas por acumulacion de cuerpos proteicos derivados de reticulos endoplasmico en plantas.
AR047658A1 (es) 2004-02-03 2006-02-01 Cargill Inc Concentrado de proteinas y corriente acuosa con carbohidratos hidrosolubles
EP2356133B1 (fr) 2008-10-10 2019-07-31 Zera Intein Protein Solutions, S.L. Corps de protéines recombinantes en tant qu'adjuvants spécifiques d'immunogènes
EP2418284A1 (fr) 2010-08-13 2012-02-15 ERA Biotech, S.A. Séquences de polypeptides induisant le corps protéiques
CN103261216A (zh) * 2010-12-10 2013-08-21 巴斯夫植物科学有限公司 经修饰的蛋白体标签及其制备方法
JP2015131781A (ja) * 2014-01-14 2015-07-23 カタギ食品株式会社 ポリクローナル抗体、ゴマアレルゲンの検出用キット、低アレルゲンゴマの判別方法、低アレルゲンゴマの作出方法及び低アレルゲンゴマ

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2744134B1 (fr) * 1996-01-29 1998-04-03 Biocem Proteines de reserve de plantes enrichies en acides amines, notamment alpha-zeine de mais enrichie en lysine plantes exprimant ces proteines

Also Published As

Publication number Publication date
BR9916731A (pt) 2001-11-06
JP2002541768A (ja) 2002-12-10
WO2000040738A1 (fr) 2000-07-13
AR016256A1 (es) 2001-06-20
EP1141347A1 (fr) 2001-10-10
AU3652299A (en) 2000-07-24
CN1334877A (zh) 2002-02-06

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Legal Events

Date Code Title Description
FZDE Discontinued