CA2343933A1 - Utilisation d'une sequence promoteur inhibiteur bifonctionnelle d'.alpha.-amylase subtilisine de l'orge pour conferer une expression dans des semences - Google Patents

Utilisation d'une sequence promoteur inhibiteur bifonctionnelle d'.alpha.-amylase subtilisine de l'orge pour conferer une expression dans des semences Download PDF

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Publication number
CA2343933A1
CA2343933A1 CA002343933A CA2343933A CA2343933A1 CA 2343933 A1 CA2343933 A1 CA 2343933A1 CA 002343933 A CA002343933 A CA 002343933A CA 2343933 A CA2343933 A CA 2343933A CA 2343933 A1 CA2343933 A1 CA 2343933A1
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CA
Canada
Prior art keywords
sequence
seq
gene
expression
plant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002343933A
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English (en)
Inventor
Agnelo Furtado
Robert James Henry
Kenneth John Scott
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SOUTHERN CROSS UNIVERSITY
University of Queensland UQ
Grains Research and Development Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2343933A1 publication Critical patent/CA2343933A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Reproductive Health (AREA)
  • Developmental Biology & Embryology (AREA)
  • Pregnancy & Childbirth (AREA)
  • General Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne un nouveau promoteur fonctionnel dans les semences de plantes, notamment l'albumen et/ou l'aleurone et/ou le scutellum. De préférence, la séquence promoteur isolée de l'invention module également l'expression d'un gène structurel en réponse aux phytohormones ABA et/ou GA. Par ailleurs ou également, la séquence promoteur de la présente invention peut être répressée ou encore régulée négativement en réponse à GA. La présente invention concerne également des constructions génétiques capables d'exprimer un gène structurel de manière fonctionnelle sous la commande de la séquence promoteur de l'invention, ainsi que des plantes transgéniques portant les constructions génétiques. La séquence promoteur de la présente invention est particulièrement utile pour modifier les traits de semences de plantes.
CA002343933A 1998-09-25 1999-09-24 Utilisation d'une sequence promoteur inhibiteur bifonctionnelle d'.alpha.-amylase subtilisine de l'orge pour conferer une expression dans des semences Abandoned CA2343933A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AUPP6175A AUPP617598A0 (en) 1998-09-25 1998-09-25 Genetic sequence and uses therefor
AUPP6175 1998-09-25
PCT/AU1999/000823 WO2000018926A1 (fr) 1998-09-25 1999-09-24 UTILISATION D'UNE SEQUENCE PROMOTEUR INHIBITEUR BIFONCTIONNELLE D'α-AMYLASE SUBTILISINE DE L'ORGE POUR CONFERER UNE EXPRESSION DANS DES SEMENCES

Publications (1)

Publication Number Publication Date
CA2343933A1 true CA2343933A1 (fr) 2000-04-06

Family

ID=3810392

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002343933A Abandoned CA2343933A1 (fr) 1998-09-25 1999-09-24 Utilisation d'une sequence promoteur inhibiteur bifonctionnelle d'.alpha.-amylase subtilisine de l'orge pour conferer une expression dans des semences

Country Status (3)

Country Link
AU (1) AUPP617598A0 (fr)
CA (1) CA2343933A1 (fr)
WO (1) WO2000018926A1 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ530094A (en) * 2001-05-31 2007-10-26 Performance Plants Inc Compositions and methods of increasing stress tolerance in plants transgenic for farnesyl transferase
AU2002952143A0 (en) * 2002-10-17 2002-10-31 Grains Research And Development Corporation Use of bifunctional alpha-amylase/subtilisin inhibitor promoter to direct expression in the pericarp of plants
FR2890859B1 (fr) 2005-09-21 2012-12-21 Oreal Oligonucleotide d'arn double brin inhibant l'expression de la tyrosinase
CN103409534B (zh) * 2013-08-16 2015-08-05 四川农业大学 检测小麦籽粒α-Amy3基因表达量的引物及其应用
CN105671060B (zh) * 2016-04-26 2019-07-23 杨燕 小麦穗发芽抗性相关基因及其应用
CN111955459B (zh) * 2020-08-20 2021-05-04 浙江大学 一种杂交水稻穗萌抑制剂及其制备方法和抑制杂交水稻穗萌的方法

Also Published As

Publication number Publication date
WO2000018926A1 (fr) 2000-04-06
AUPP617598A0 (en) 1998-10-22

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Legal Events

Date Code Title Description
FZDE Discontinued