CA2331921A1 - Nouvel adnc mucin intestinal des invertebres et produits et procedes correspondants - Google Patents

Nouvel adnc mucin intestinal des invertebres et produits et procedes correspondants Download PDF

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Publication number
CA2331921A1
CA2331921A1 CA002331921A CA2331921A CA2331921A1 CA 2331921 A1 CA2331921 A1 CA 2331921A1 CA 002331921 A CA002331921 A CA 002331921A CA 2331921 A CA2331921 A CA 2331921A CA 2331921 A1 CA2331921 A1 CA 2331921A1
Authority
CA
Canada
Prior art keywords
protein
iim
intestinal mucin
pro
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002331921A
Other languages
English (en)
Inventor
Robert R. Granados
Ping Wang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boyce Thompson Institute for Plant Research Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/103,429 external-priority patent/US6187558B1/en
Application filed by Individual filed Critical Individual
Publication of CA2331921A1 publication Critical patent/CA2331921A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/13—Immunoglobulins specific features characterized by their source of isolation or production isolated from plants
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Insects & Arthropods (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Pest Control & Pesticides (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

L'invention concerne un nouvel ADNc mucin intestinal de l'insecte. La protéine mucine intestinale de l'insecte (MII) a été identifiée et clonée au moyen de larves de T. ni. On a déterminé et découvert les séquences d'acides aminés et l'ADNc. La nouvelle protéine possède une masse moléculaire d'environ 400 kDa. Ces séquences peuvent servir à la production de vecteurs transgéniques ou recombinants, y compris des virus, des micro-organismes, des cellules, des plantes ou des animaux, lesdits virus, micro-organismes, cellules, plantes ou animaux étant le produit de l'insertion d'un vecteur d'expression génique comprenant un ADN qui code pour la séquence de la protéine MII. De ce fait, l'hôte obtenu par génie génétique de la séquence d'ADN MII est capable d'exprimer cette protéine MII sous une forme fonctionnelle. On a également trouvé utile une séquence purifiée et isolée d'ADN recombinant qui comprend une séquence d'ADN codant pour une protéine MII. La séquence utilisée d'ADN recombinant peut être une séquence d'ADNc pour MII 14 ou MII 22, SEQ. ID. Nos. 1 et 2, respectivement. La présente invention concerne également l'utilisation des séquences purifiées d'acides aminés pour MII 14 ou MII 22, SEQ. ID. Nos. 3 et 4, respectivement. Grâce à cette invention, la connaissance des composants protéiniques de la membrane péritrophique, et en particulier des protéines similaires à la mucine, on pourra améliorer l'efficacité des biopesticides obtenus par génie biologique et des vecteurs viraux recombinants, renforcer les défenses des plantes transgéniques ou protéger les insectes vecteurs vulnérables aux attaques des organismes utilisant l'enzyme "enhancine" ou similaires.
CA002331921A 1998-06-24 1999-06-23 Nouvel adnc mucin intestinal des invertebres et produits et procedes correspondants Abandoned CA2331921A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US09/103,429 1998-06-24
US09/103,429 US6187558B1 (en) 1998-06-24 1998-06-24 Invertebrate intestinal mucin cDNA and related products and methods
US09/294,663 1999-04-19
US09/294,663 US6765127B1 (en) 1998-06-24 1999-04-19 Invertebrate intestinal mucin cDNA and related products and methods
PCT/US1999/014220 WO1999067373A2 (fr) 1998-06-24 1999-06-23 Nouvel adnc mucin intestinal des invertebres et produits et procedes correspondants

Publications (1)

Publication Number Publication Date
CA2331921A1 true CA2331921A1 (fr) 1999-12-29

Family

ID=26800455

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002331921A Abandoned CA2331921A1 (fr) 1998-06-24 1999-06-23 Nouvel adnc mucin intestinal des invertebres et produits et procedes correspondants

Country Status (3)

Country Link
AU (1) AU4710899A (fr)
CA (1) CA2331921A1 (fr)
WO (1) WO1999067373A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SK11212002A3 (sk) * 2000-02-04 2003-01-09 Soci�T� Des Produits Nestl� S.A. Použitie proteínového zdroja, spôsob zachovania syntézy mucínov v tele pacienta a výživový prípravok
EP3415010A1 (fr) 2017-06-13 2018-12-19 Agrosavfe Nv Polypeptides anti-insectes et procédés

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ224663A (en) * 1987-05-28 1990-11-27 Amrad Corp Ltd Fusion proteins containing glutathione-s-transferase and expression of foreign polypeptides using glutathione-s-transferase encoding vectors
US5686600A (en) * 1994-06-28 1997-11-11 Novartis Finance Corporation Antibodies which bind to insect gut proteins and their use

Also Published As

Publication number Publication date
WO1999067373A2 (fr) 1999-12-29
WO1999067373A3 (fr) 2000-07-20
AU4710899A (en) 2000-01-10

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