CA2330233A1 - Genes codant pour les polypeptides de .beta.-galactosidase de la tomate - Google Patents

Genes codant pour les polypeptides de .beta.-galactosidase de la tomate Download PDF

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Publication number
CA2330233A1
CA2330233A1 CA002330233A CA2330233A CA2330233A1 CA 2330233 A1 CA2330233 A1 CA 2330233A1 CA 002330233 A CA002330233 A CA 002330233A CA 2330233 A CA2330233 A CA 2330233A CA 2330233 A1 CA2330233 A1 CA 2330233A1
Authority
CA
Canada
Prior art keywords
seq
galactosidase
nucleic acid
beta
nucleotide sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002330233A
Other languages
English (en)
Inventor
Kenneth C. Gross
David L. Smith
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
US Department of Agriculture USDA
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2330233A1 publication Critical patent/CA2330233A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne une nouvelle famille de gènes de .beta.-galactosidase, ainsi que les séquences d'ADN dérivées des clones d'ADN complémentaire de ces gènes. Cette invention concerne par exemple une protéine de .beta.-galactosidase II codée par un clone de l'ADN complémentaire, ci-après dénommé pZBG2-1-4. L'invention concerne également un procédé permettant de modifier le métabolisme pariétal, ce procédé consistant à modifier l'activité d'au moins une .beta.-galactosidase, et donc à modifier la qualité du fruit. La présente invention concerne par ailleurs un produit de recombinaison d'ADN comprenant la totalité ou une partie de la séquence d'ADN d'une .beta.-galactosidase, sous le contrôle d'une région d'initiation transcriptionnelle végétale, de sorte que ledit produit de recombinaison peut produire un ARN, et éventuellement un polypeptide de .beta.-galactosidase, dans des cellules végétales. La présente invention concerne en outre des vecteurs recombinants comprenant les molécules d'acide nucléique isolé de cette invention, ainsi que des cellules hôtes contenant ces vecteurs recombinants. Cette invention concerne également des procédés de fabrication de ces vecteurs et de ces cellules hôtes, ainsi que leur procédé d'utilisation permettant de produire des polypeptides ou des peptides de .beta.-galactosidase par des techniques de recombinaison. La présente invention concerne enfin: des cellules végétales contenant les produits de recombinaison d'ADN susmentionnés; des végétaux dérivés de ces cellules et présentant une expression génique de .beta.-galactosidase modifiée; et des graines produites à partir de ces végétaux.
CA002330233A 1998-06-09 1999-06-08 Genes codant pour les polypeptides de .beta.-galactosidase de la tomate Abandoned CA2330233A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US8880598P 1998-06-09 1998-06-09
US60/088,805 1998-06-09
PCT/US1999/012697 WO1999064564A1 (fr) 1998-06-09 1999-06-08 GENES CODANT POUR LES POLYPEPTIDES DE β-GALACTOSIDASE DE LA TOMATE

Publications (1)

Publication Number Publication Date
CA2330233A1 true CA2330233A1 (fr) 1999-12-16

Family

ID=22213590

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002330233A Abandoned CA2330233A1 (fr) 1998-06-09 1999-06-08 Genes codant pour les polypeptides de .beta.-galactosidase de la tomate

Country Status (12)

Country Link
EP (1) EP1084228A1 (fr)
JP (1) JP2002517226A (fr)
CN (1) CN1311816A (fr)
AU (1) AU778691B2 (fr)
BR (1) BR9911074A (fr)
CA (1) CA2330233A1 (fr)
CZ (1) CZ20004594A3 (fr)
HU (1) HUP0102605A2 (fr)
MX (1) MXPA00012210A (fr)
NZ (1) NZ508968A (fr)
PL (1) PL345269A1 (fr)
WO (1) WO1999064564A1 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT102511B (pt) * 2000-08-22 2007-08-01 Inst De Ciencia Aplic E Tecnol Genes codificantes de b-galactosidase, pectinametilesterase, poligalacturonase eexpansinas isolados de pêra
CN100370029C (zh) * 2005-10-11 2008-02-20 山东大学 一种高效转糖基β-半乳糖苷酶基因
GB0601901D0 (en) * 2006-01-31 2006-03-08 Product and Process
GB0606112D0 (en) * 2006-03-28 2006-05-03 Product and process
CN114457097A (zh) * 2021-12-27 2022-05-10 河南农业大学 苹果β-半乳糖苷酶基因Mdβ-gal18及在调控果实成熟软化中的应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9320930D0 (en) * 1993-10-12 1993-12-01 Zeneca Ltd Modified fruit
GB9403423D0 (en) * 1994-02-23 1994-04-13 Unilever Plc Novel exo-(1-4)- beta-D galactanase

Also Published As

Publication number Publication date
CZ20004594A3 (cs) 2002-05-15
EP1084228A1 (fr) 2001-03-21
MXPA00012210A (es) 2002-10-17
PL345269A1 (en) 2001-12-03
WO1999064564A1 (fr) 1999-12-16
HUP0102605A2 (hu) 2001-11-28
NZ508968A (en) 2003-10-31
BR9911074A (pt) 2002-01-02
CN1311816A (zh) 2001-09-05
JP2002517226A (ja) 2002-06-18
AU4549899A (en) 1999-12-30
AU778691B2 (en) 2004-12-16

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued