CA2321119A1 - Procede permettant d'identifier les sites reactifs des glycosylases de reparation des mesappariements, compose et utilisations de ce dernier - Google Patents

Procede permettant d'identifier les sites reactifs des glycosylases de reparation des mesappariements, compose et utilisations de ce dernier Download PDF

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Publication number
CA2321119A1
CA2321119A1 CA002321119A CA2321119A CA2321119A1 CA 2321119 A1 CA2321119 A1 CA 2321119A1 CA 002321119 A CA002321119 A CA 002321119A CA 2321119 A CA2321119 A CA 2321119A CA 2321119 A1 CA2321119 A1 CA 2321119A1
Authority
CA
Canada
Prior art keywords
dna
mismatch
compound
mismatches
duplex
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002321119A
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English (en)
Inventor
G. Mike Makrigiorgos
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dana Farber Cancer Institute Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/224,227 external-priority patent/US6174680B1/en
Application filed by Individual filed Critical Individual
Publication of CA2321119A1 publication Critical patent/CA2321119A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pyrane Compounds (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)

Abstract

La présente invention concerne un procédé permettant d'identifier des mutations dans une séquence d'ADN cible. Ledit procédé consiste à: (a) hybrider la séquence d'ADN cible avec une séquence d'ADN de contrôle, la séquence d'ADN de contrôle étant une séquence d'ADN de type sauvage correspondant à la séquence d'ADN cible afin de créer un duplex; (b) traiter le duplex pour enlever les aldéhydes spontanés; (c) mettre à réagir le duplex avec une glycosylase de réparation pour transformer les éventuels sites de mésappariement du duplex en sites réactifs contenant un site abasique renfermant un aldéhyde; (d) mettre à réagir le duplex avec un composé de la formule X-Z-Y, dans laquelle X est un fragment détectable, Y est NHNH¿2?, O-NH¿2? ou NH¿2?, et Z est un hydrocarbure, un alkylhydroxy, un alkyléthoxy, un alkylester, un alkyléther, un alkylamide ou une alkylamine, Z pouvant être substitué ou non; et dans laquelle Z peut renfermer un groupe clivable; pendant une durée assez longue et dans des conditions suffisantes pour provoquer une liaison covalente avec les sites réactifs; (e) détecter le composé lié pour identifier les sites de mésappariement; (f) déterminer l'emplacement des mésappariements; et (g) déterminer si le mésappariement est une mutation ou un polymorphisme.
CA002321119A 1998-02-23 1999-02-23 Procede permettant d'identifier les sites reactifs des glycosylases de reparation des mesappariements, compose et utilisations de ce dernier Abandoned CA2321119A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US7554298P 1998-02-23 1998-02-23
US60/075,542 1998-02-23
US09/224,227 US6174680B1 (en) 1998-12-30 1998-12-30 Method for identifying mismatch repair glycosylase reactive sites, compounds and uses thereof
US09/224,227 1998-12-30
PCT/US1999/003821 WO1999042622A1 (fr) 1998-02-23 1999-02-23 Procede permettant d'identifier les sites reactifs des glycosylases de reparation des mesappariements, compose et utilisations de ce dernier

Publications (1)

Publication Number Publication Date
CA2321119A1 true CA2321119A1 (fr) 1999-08-26

Family

ID=26756994

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002321119A Abandoned CA2321119A1 (fr) 1998-02-23 1999-02-23 Procede permettant d'identifier les sites reactifs des glycosylases de reparation des mesappariements, compose et utilisations de ce dernier

Country Status (5)

Country Link
EP (1) EP1060266A4 (fr)
JP (1) JP2002504352A (fr)
AU (1) AU756384B2 (fr)
CA (1) CA2321119A1 (fr)
WO (1) WO1999042622A1 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002538415A (ja) * 1998-12-30 2002-11-12 デイナ−ファーバー キャンサー インスティチュート，インコーポレイテッド 変異走査アレイ、およびその使用方法
FR2798138B1 (fr) * 1999-09-03 2004-05-21 Centre Nat Rech Scient Clonage, expression et caracterisation du gene spg4 responsable de la forme la plus frequente de paraplegie spastique autosomique dominante
US6365355B1 (en) * 2000-03-28 2002-04-02 The Regents Of The University Of California Chimeric proteins for detection and quantitation of DNA mutations, DNA sequence variations, DNA damage and DNA mismatches
EP1319081A2 (fr) * 2000-09-18 2003-06-18 Pioneer Hi-Bred International, Inc. Orthologue mlh1 du riz et utilisation de celui-ci
AU2002215345A1 (en) * 2000-10-13 2002-04-22 Eos Biotechnology, Inc. Methods of diagnosis of prostate cancer, compositions and methods of screening for modulators of prostate cancer
US20050089852A1 (en) * 2001-03-14 2005-04-28 Lee Shao C. Method and compositions for evaluating risk of developing type 2 diabetes in people of chinese descent
DE10159904A1 (de) * 2001-12-06 2003-07-03 Adnagen Ag Oligonukleotidanordnung, Verfahren zum Nukleotidnachweis sowie Vorrichtung hierfür

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3315116A1 (de) * 1983-03-23 1984-09-27 Europäische Atomgemeinschaft (EURATOM), Luxembourg Verfahren zur direkten bestimmung apurinischer und apyrimidinischer stellen in dna
US5516663A (en) * 1990-01-26 1996-05-14 Abbott Laboratories Ligase chain reaction with endonuclease IV correction and contamination control
CA2087724C (fr) * 1990-07-24 2003-09-16 John J. Sninsky Reduction de l'amplification non specifique lors de l'amplification de l'acide nucleique in vitro grace a des bases d'acide nucleique modifiees
WO1992007951A1 (fr) * 1990-10-19 1992-05-14 Yoke Wah Kow Quantification de lesions contenant un aldehyde dans des acides nucleiques
CA2226542C (fr) * 1995-07-11 2006-04-04 Thomas Valentine Mccarthy Detection de sequences nucleotidiques induite par la glycosylase sur des loci potentiels
US5719031A (en) * 1996-08-14 1998-02-17 Molecular Probes, Inc. Dye labeled polymers as reagents for measuring polymer degradation

Also Published As

Publication number Publication date
JP2002504352A (ja) 2002-02-12
AU756384B2 (en) 2003-01-09
EP1060266A1 (fr) 2000-12-20
EP1060266A4 (fr) 2004-09-22
AU2780799A (en) 1999-09-06
WO1999042622A1 (fr) 1999-08-26

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Legal Events

Date Code Title Description
FZDE Discontinued