CA2320896A1 - Expression of trehalose biosynthetic genes in plants - Google Patents

Expression of trehalose biosynthetic genes in plants Download PDF

Info

Publication number
CA2320896A1
CA2320896A1 CA002320896A CA2320896A CA2320896A1 CA 2320896 A1 CA2320896 A1 CA 2320896A1 CA 002320896 A CA002320896 A CA 002320896A CA 2320896 A CA2320896 A CA 2320896A CA 2320896 A1 CA2320896 A1 CA 2320896A1
Authority
CA
Canada
Prior art keywords
plant
promoter
gene
trehalose
nucleotide sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002320896A
Other languages
English (en)
French (fr)
Inventor
Edouard Guillaume Lebel
Peter Bernard Heifetz
Stephen Arthur Goff
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Syngenta Participations AG
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2320896A1 publication Critical patent/CA2320896A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
    • C12N9/1051Hexosyltransferases (2.4.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214Plastid transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8217Gene switch
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223Vegetative tissue-specific promoters
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237Externally regulated expression systems
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA002320896A 1998-03-11 1999-03-09 Expression of trehalose biosynthetic genes in plants Abandoned CA2320896A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US7766598P 1998-03-11 1998-03-11
US60/077,665 1998-03-11
PCT/EP1999/001516 WO1999046370A2 (en) 1998-03-11 1999-03-09 Expression of trehalose biosynthetic genes in plants

Publications (1)

Publication Number Publication Date
CA2320896A1 true CA2320896A1 (en) 1999-09-16

Family

ID=22139381

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002320896A Abandoned CA2320896A1 (en) 1998-03-11 1999-03-09 Expression of trehalose biosynthetic genes in plants

Country Status (15)

Country Link
US (2) US6686516B2 (en:Method)
EP (1) EP1064365A2 (en:Method)
JP (1) JP2002505875A (en:Method)
KR (1) KR20010082520A (en:Method)
CN (1) CN1292824A (en:Method)
AU (1) AU748604B2 (en:Method)
BR (1) BR9909258A (en:Method)
CA (1) CA2320896A1 (en:Method)
EA (1) EA200000926A1 (en:Method)
HU (1) HUP0102598A3 (en:Method)
IL (1) IL138191A0 (en:Method)
IN (1) IN2000CN00368A (en:Method)
PL (1) PL343460A1 (en:Method)
TR (1) TR200002624T2 (en:Method)
WO (1) WO1999046370A2 (en:Method)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7214858B2 (en) * 1999-03-31 2007-05-08 E. I. Du Pont De Nemours And Company Plant genes encoding trehalose metabolism enzymes
AU6277600A (en) * 1999-07-27 2001-02-13 Syngenta Participations Ag Novel chimeric genes
EP1632576B1 (en) 1999-12-16 2017-05-31 Monsanto Technology, LLC Novel plant expression constructs
AU2001241809A1 (en) * 2000-02-29 2001-09-12 Auburn University Genetic engineering of drought tolerance via a plastid genome
US20040064846A1 (en) * 2001-02-28 2004-04-01 Henry Daniell Genetic engineering of drought tolerance via a plastid genome
EP1576101A4 (en) * 2002-04-23 2007-10-31 Scripps Research Inst EXPRESSION OF POLYPEPTIDES IN CHLOROPLASTS AND COMPOSITIONS AND METHODS OF EXPRESSION THEREOF
EP1375669A1 (en) * 2002-06-13 2004-01-02 Stichting Voor De Technische Wetenschappen Method for enhancing the disease resistance in plants by altering trehalose-6-phosphate levels
KR100440725B1 (ko) * 2002-06-20 2004-07-15 주식회사 그린진 바이오텍 비생물성 스트레스에 대한 단자엽 식물의 내성을증가시키는 방법
WO2004044143A2 (en) * 2002-11-06 2004-05-27 Cornell Research Foundation, Inc. Tps plant gene constructs and transformants
AU2003290635A1 (en) * 2002-11-08 2004-06-03 Paradigm Genetics, Inc. Methods for the identification of trehalose-6-phosphate synthase as antibiotics
AU2005235311B2 (en) * 2004-04-20 2009-10-08 Syngenta Participations Ag Regulatory sequences for expressing gene products in plant reproductive tissue
BRPI0518596A2 (pt) * 2004-12-03 2008-11-25 Syngenta Participations Ag tolerÂncia ao estresse em plantas atravÉs da inibiÇço seletiva da trealose-6-fosfato fosfatase
CN100355883C (zh) * 2004-12-10 2007-12-19 中国科学院上海生命科学研究院 水稻抗逆相关基因-海藻糖-6-磷酸磷酸化酶基因及其应用
CA2592704C (en) 2005-01-07 2016-05-24 E. I. Du Pont De Nemours And Company Nucleotide sequences encoding ramosa3 and sister of ramosa3 and methods of use for same
DE602005027621D1 (de) 2005-09-06 2011-06-01 Stichting Dienst Landbouwkundi Verwendung einer Nukleinsäure zur Herstellung einer transgenen Pflanze mit verbesserter Toleranz gegenüber Trockenheit
CN103298942A (zh) * 2007-04-03 2013-09-11 桌面能源公司 用于转化真核藻类的方法
US8268553B2 (en) 2007-06-01 2012-09-18 Sapphire Energy, Inc. High throughput screening of genetically modified photosynthetic organisms
WO2009017821A1 (en) * 2007-08-01 2009-02-05 Bioglow Inc. Bioluminescent plants comprising bacterial lux operon and methods of making same
CL2008002680A1 (es) * 2007-09-11 2009-10-16 Sapphire Energy Inc Composicion que comprende moleculas con atomos de hidrogeno y carbono que representan a lo menos 80% del peso de la composicion, y donde la distribucion de carbono 13 de la composicion es inferior a -32 0/00; producto combustible que comprende la composicion, y metodo para la generacion de dioxido de carbono.
NZ583701A (en) * 2007-09-11 2012-03-30 Sapphire Energy Inc Molecule production by photosynthetic organisms
CA2713208C (en) 2008-02-15 2021-02-16 Ceres, Inc. Drought and heat tolerance in plants
CN101289514B (zh) * 2008-06-13 2011-01-19 北京北方杰士生物科技有限责任公司 一种培育耐逆植物的方法及其专用dna片段
US9175300B2 (en) 2008-11-27 2015-11-03 Vanga Siva Reddy Method of expressing foreign protein in plastids
AU2010309990A1 (en) * 2009-10-22 2012-05-24 Basf Plant Science Company Gmbh Plants having enhanced yield-related traits and a method for making the same
JP2014527817A (ja) * 2011-09-13 2014-10-23 ストーラー エンタープライズィズ,インク トレハロースの使用による作物収穫高の増大方法
AU2012332343A1 (en) 2011-11-03 2014-05-22 Syngenta Participations Ag Polynucleotides, polypeptides and methods for enhancing photossimilation in plants
GB201609969D0 (en) 2016-06-07 2016-07-20 Univ Cape Town Drought resistance multigene construct
CN106801102B (zh) * 2017-02-23 2020-11-20 浙江省检验检疫科学技术研究院 苋属的实时荧光pcr鉴定方法
HUE065734T2 (hu) * 2017-05-04 2024-06-28 Cargill Inc Genetikailag módosított trehaláz-kiválasztó élesztõk és az ilyen genetikailag módosított élesztõket alkalmazó erjesztési eljárások
US11242536B2 (en) * 2017-09-26 2022-02-08 Rutgers, The State University Of New Jersey Quality protein maize based on increased sulfur reduction in leaf cells
CN108624598A (zh) * 2018-06-06 2018-10-09 福建农林大学 Tppf基因在调控植物海藻糖合成和提高植物抗旱性中的应用
CN108728457B (zh) * 2018-06-22 2021-09-17 山东省药学科学院 一种海藻糖合成酶基因优化序列及其应用

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5614395A (en) * 1988-03-08 1997-03-25 Ciba-Geigy Corporation Chemically regulatable and anti-pathogenic DNA sequences and uses thereof
US5693507A (en) * 1988-09-26 1997-12-02 Auburn University Genetic engineering of plant chloroplasts
US5422254A (en) * 1992-02-14 1995-06-06 Oy Alko Ab Method to increase the trehalose content of organisms by transforming them with the structural genes for the short and long chains of yeast trehalose synthase
FI943133A0 (fi) * 1994-06-29 1994-06-29 Alko Ab Oy Transgena vaexter
US5792921A (en) * 1992-02-14 1998-08-11 Londesborough; John Increasing the trehalose content of organisms by transforming them with combinations of the structural genes for trehalose synthase
DE69434173T2 (de) 1993-06-30 2005-05-19 Syngenta Mogen B.V. Herstellung von trehalose in pflanzen
US5780709A (en) * 1993-08-25 1998-07-14 Dekalb Genetics Corporation Transgenic maize with increased mannitol content
ATE317445T1 (de) * 1993-11-19 2006-02-15 Biotechnology Res & Dev Chimäre regulatorische regionen und gen - kassetten zur genexpression in pflanzen
US5545818A (en) * 1994-03-11 1996-08-13 Calgene Inc. Expression of Bacillus thuringiensis cry proteins in plant plastids
US5925806A (en) * 1995-06-06 1999-07-20 Mcbride; Kevin E. Controlled expression of transgenic constructs in plant plastids
MX205414B (es) * 1996-05-08 2001-12-07 Univ Mexico Nacional Autonoma Metodo para incrementar de trehalosa de los organismos por medio de su transformacion con el adnc de la trehalosa-6-fosfato sintasa/fosfatasa de selaginella lepidophylla.
AU708850B2 (en) * 1996-07-23 1999-08-12 Novartis Ag Chemically-inducible arabidopsis PR-1 promoter
ZA989886B (en) * 1997-10-30 1999-04-30 Mogen Int Nuclear male sterile plants method of producing same and methods to restore fertility

Also Published As

Publication number Publication date
US6686516B2 (en) 2004-02-03
US20040078848A1 (en) 2004-04-22
WO1999046370A2 (en) 1999-09-16
WO1999046370A3 (en) 1999-11-18
US20030009784A1 (en) 2003-01-09
IN2000CN00368A (en:Method) 2010-03-19
CN1292824A (zh) 2001-04-25
HUP0102598A2 (hu) 2001-11-28
BR9909258A (pt) 2000-11-28
JP2002505875A (ja) 2002-02-26
IL138191A0 (en) 2001-10-31
US7030294B2 (en) 2006-04-18
PL343460A1 (en) 2001-08-13
AU748604B2 (en) 2002-06-06
EA200000926A1 (ru) 2001-06-25
AU3329599A (en) 1999-09-27
KR20010082520A (ko) 2001-08-30
TR200002624T2 (tr) 2000-11-21
HUP0102598A3 (en) 2003-03-28
EP1064365A2 (en) 2001-01-03

Similar Documents

Publication Publication Date Title
AU748604B2 (en) Expression of trehalose biosynthetic genes in plants
EP1516931B1 (en) DSRNA-mediated regulation of gene expression in plants
EP1801215B1 (en) Regulation of viral gene expression
CA2322016A1 (en) Genes encoding mlo proteins and conferring fungal resistance upon plants
US5753507A (en) Plant geraniol/nerol 10-hydroxylase and DNA coding therefor
US5869719A (en) Transgenic plants having increased biotin content
AU773514B2 (en) Thioredoxin and grain processing
US7019195B1 (en) Method for conferring resistance or tolerance aganist furovirus, potyvirus, tospovirus, and cucomovirus to plant cells
AU700943B2 (en) Enhanced biotin biosynthesis in plant tissue
MXPA00008809A (en) Expression of trehalose biosynthetic genes in plants
HK1108917A (en) Regulation of viral gene expression
HK1034999B (en) Dsrna-mediated regulation of gene expression in plants

Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued