CA2317941A1 - Vecteurs d'adenovirus comprenant des proteines capsidiques modifiees - Google Patents

Vecteurs d'adenovirus comprenant des proteines capsidiques modifiees Download PDF

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Publication number
CA2317941A1
CA2317941A1 CA002317941A CA2317941A CA2317941A1 CA 2317941 A1 CA2317941 A1 CA 2317941A1 CA 002317941 A CA002317941 A CA 002317941A CA 2317941 A CA2317941 A CA 2317941A CA 2317941 A1 CA2317941 A1 CA 2317941A1
Authority
CA
Canada
Prior art keywords
adenoviral
protein
cells
adenoviral vector
capsid protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002317941A
Other languages
English (en)
Inventor
Helen Romanczuk
Donna Armentano
Catherine R. O'riordan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genzyme Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2317941A1 publication Critical patent/CA2317941A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10345—Special targeting system for viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2810/00—Vectors comprising a targeting moiety
    • C12N2810/40—Vectors comprising a peptide as targeting moiety, e.g. a synthetic peptide, from undefined source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2810/00—Vectors comprising a targeting moiety
    • C12N2810/40—Vectors comprising a peptide as targeting moiety, e.g. a synthetic peptide, from undefined source
    • C12N2810/405—Vectors comprising RGD peptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2810/00—Vectors comprising a targeting moiety
    • C12N2810/50—Vectors comprising as targeting moiety peptide derived from defined protein
    • C12N2810/60—Vectors comprising as targeting moiety peptide derived from defined protein from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2810/00—Vectors comprising a targeting moiety
    • C12N2810/50—Vectors comprising as targeting moiety peptide derived from defined protein
    • C12N2810/60—Vectors comprising as targeting moiety peptide derived from defined protein from viruses
    • C12N2810/6009—Vectors comprising as targeting moiety peptide derived from defined protein from viruses dsDNA viruses
    • C12N2810/6018—Adenoviridae

Abstract

Cette invention concerne des vecteurs d'adénovirus comportant des protéines capsidiques modifiées qui comprennent des ligands hétérologues qui améliorent et/ou altèrent le potentiel infectieux du vecteur. Ces ligands sont capables de se lier à des cellules cibles, et leur inclusion dans des vecteurs d'adénovirus facilite la liaison et le potentiel infectieux des vecteurs. Dans une forme de réalisation préférée, les ligands sont des peptides et les cellules cibles sont des cellules épithéliales. Cette invention concerne également de nouveaux ligands hétérologues, des complexes ligand-récepteur, et des compositions contenant les vecteurs d'adénovirus de cette invention. D'autres aspects de l'invention portent sur des procédés d'utilisation des vecteurs d'adénovirus de cette invention pour apporter des transgènes à des cellules cibles.
CA002317941A 1998-01-16 1999-01-15 Vecteurs d'adenovirus comprenant des proteines capsidiques modifiees Abandoned CA2317941A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US7167498P 1998-01-16 1998-01-16
US60/071,674 1998-01-16
PCT/US1999/000913 WO1999036545A2 (fr) 1998-01-16 1999-01-15 Vecteurs d'adenovirus comprenant des proteines capsidiques modifiees

Publications (1)

Publication Number Publication Date
CA2317941A1 true CA2317941A1 (fr) 1999-07-22

Family

ID=22102858

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002317941A Abandoned CA2317941A1 (fr) 1998-01-16 1999-01-15 Vecteurs d'adenovirus comprenant des proteines capsidiques modifiees

Country Status (5)

Country Link
EP (1) EP1044274A2 (fr)
JP (1) JP2002508967A (fr)
AU (1) AU2321999A (fr)
CA (1) CA2317941A1 (fr)
WO (1) WO1999036545A2 (fr)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU4070499A (en) 1998-04-30 1999-11-16 Cornell Research Foundation Inc. Adenoviral vectors with tandem fiber proteins
US6455314B1 (en) 1998-09-11 2002-09-24 Genvec, Inc. Alternatively targeted adenovirus
AU7085500A (en) * 1999-09-08 2001-04-10 Genzyme Corporation Adenoviral vectors modified for increased and persistent expression of the cystic fibrosis transmembrane conductance regulator gene in human airway epithelia
US6955808B2 (en) 1999-09-24 2005-10-18 Uab Research Foundation Capsid-modified recombinant adenovirus and methods of use
ATE345819T1 (de) * 1999-09-24 2006-12-15 Uab Research Foundation Kapsidmodifiziertes rekombinantes adenovirusund verfahren zu dessen anwendung
WO2001058940A2 (fr) * 2000-02-09 2001-08-16 Genvec, Inc. Capside adenovirale contenant une proteine ix chimere
AU2000239309A1 (en) * 2000-03-29 2001-10-08 Dgi Biotechnologies Llc Insulin and igf-1 receptor agonists and antagonists
AU2001263689B2 (en) * 2000-05-31 2007-03-22 University Of Saskatchewan Modified bovine adenovirus having altered tropism
US20020168343A1 (en) * 2001-02-14 2002-11-14 Curiel David T. Combined transductional and transcriptional targeting system for improved gene delivery
WO2002083880A1 (fr) * 2001-04-17 2002-10-24 Vectorlogics, Inc. Vecteurs adenoviraux en mosaique
CA2448908C (fr) * 2001-05-30 2008-03-18 Transgene S.A. Proteine d'adenovirus ix, ses domaines participant a l'ensemble de capside, activite transcriptionnelle et reorganisation nucleaire
MXPA05013234A (es) * 2003-06-10 2006-03-09 Univ Saskatchewan Proteinas de capside de adenovirus quimericas.
EP2036980A1 (fr) 2007-09-14 2009-03-18 Gruber, Jens Dérégulation de l'expression génétique à l'aide de particules semblables à un virus chargées d'acide nucléique
EP2252316A2 (fr) * 2008-02-07 2010-11-24 Andrew Baker Modulation du tropisme adénoviral
CZ2008565A3 (cs) * 2008-09-15 2009-11-18 Vysoká škola chemicko technologická v Praze Príprava monoklonálních protilátek proti FoxP3 proteinu s použitím modifikovaných virum podobných cástic Masonova-Pfizerova opicího viru
EP2563387B1 (fr) * 2010-04-28 2014-02-26 Institut Gustave Roussy Utilisation médicale de vecteurs vaccinaux à base d'adénovirus
CN102559758B (zh) * 2011-12-16 2014-10-22 陕西师范大学 制备六联体蛋白经遗传修饰的腺病毒的载体及其构建方法
US9217159B2 (en) 2012-05-18 2015-12-22 The Trustees Of The University Of Pennsylvania Subfamily E simian adenoviruses A1302, A1320, A1331 and A1337 and uses thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5543328A (en) * 1993-08-13 1996-08-06 Genetic Therapy, Inc. Adenoviruses having modified fiber proteins
US6312699B1 (en) * 1994-03-28 2001-11-06 Uab Research Foundation Ligands added to adenovirus fiber
BR9612685A (pt) * 1995-11-28 1999-07-20 Genvec Inc Vetores e métodos para transferéncia de gene para células
US5871727A (en) * 1995-12-08 1999-02-16 Uab Research Foundation Targeted adenovirus vectors
US5877011A (en) * 1996-11-20 1999-03-02 Genzyme Corporation Chimeric adenoviral vectors
FR2761688A1 (fr) * 1997-04-02 1998-10-09 Transgene Sa Fibre adenovirale modifiee et adenovirus cibles

Also Published As

Publication number Publication date
WO1999036545A2 (fr) 1999-07-22
AU2321999A (en) 1999-08-02
EP1044274A2 (fr) 2000-10-18
WO1999036545A3 (fr) 1999-11-04
JP2002508967A (ja) 2002-03-26

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