CA2305341C - Stabilisation des glycoproteines trimeres d'enveloppe au moyen de liaisons bisulfure introduites dans un ectodomaine de la glycoproteine gp41 - Google Patents

Stabilisation des glycoproteines trimeres d'enveloppe au moyen de liaisons bisulfure introduites dans un ectodomaine de la glycoproteine gp41 Download PDF

Info

Publication number
CA2305341C
CA2305341C CA002305341A CA2305341A CA2305341C CA 2305341 C CA2305341 C CA 2305341C CA 002305341 A CA002305341 A CA 002305341A CA 2305341 A CA2305341 A CA 2305341A CA 2305341 C CA2305341 C CA 2305341C
Authority
CA
Canada
Prior art keywords
hiv
protein
derivative
glycoprotein
coiled coil
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA002305341A
Other languages
English (en)
Other versions
CA2305341A1 (fr
Inventor
Joseph G. Sodroski
Michael Farzan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dana Farber Cancer Institute Inc
Original Assignee
Dana Farber Cancer Institute Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dana Farber Cancer Institute Inc filed Critical Dana Farber Cancer Institute Inc
Publication of CA2305341A1 publication Critical patent/CA2305341A1/fr
Application granted granted Critical
Publication of CA2305341C publication Critical patent/CA2305341C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/18—Antivirals for RNA viruses for HIV
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16111—Human Immunodeficiency Virus, HIV concerning HIV env
    • C12N2740/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Virology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • AIDS & HIV (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention se rapporte à des glycoprotéines trimères d'enveloppe stabilisés. Ces trimères sont stabilisés par l'introduction de liaisons bisulfure dans certains sites de l'ectodomaine de gp41. On peut utiliser les molécules d'ADN codant ces trimères pour déclencher une réponse immunogénique.
CA002305341A 1997-10-01 1998-10-01 Stabilisation des glycoproteines trimeres d'enveloppe au moyen de liaisons bisulfure introduites dans un ectodomaine de la glycoproteine gp41 Expired - Fee Related CA2305341C (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US6081397P 1997-10-01 1997-10-01
US60/060,813 1997-10-01
US6080897P 1997-10-03 1997-10-03
US60/060,808 1997-10-03
PCT/US1998/020693 WO1999016883A2 (fr) 1997-10-01 1998-10-01 Stabilisation des glycoproteines trimeres d'enveloppe au moyen de liaisons bisulfure introduites dans un ectodomaine de la glycoproteine gp41

Publications (2)

Publication Number Publication Date
CA2305341A1 CA2305341A1 (fr) 1999-04-08
CA2305341C true CA2305341C (fr) 2009-01-06

Family

ID=26740384

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002305341A Expired - Fee Related CA2305341C (fr) 1997-10-01 1998-10-01 Stabilisation des glycoproteines trimeres d'enveloppe au moyen de liaisons bisulfure introduites dans un ectodomaine de la glycoproteine gp41

Country Status (4)

Country Link
EP (1) EP1019511A2 (fr)
AU (1) AU9678598A (fr)
CA (1) CA2305341C (fr)
WO (1) WO1999016883A2 (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2781676B1 (fr) * 1998-07-31 2004-04-02 Pasteur Merieux Serums Vacc Trimere du produit d'expression du gene env de hiv
CA2384271A1 (fr) 1999-09-17 2001-03-22 Joseph G. Sodroski Trimeres stabilises solubles de glycoproteines
US7179468B1 (en) 2000-06-08 2007-02-20 Cornell Research Foundation, Inc. Antigen for developing neutralizing antibodies to human immunodeficiency virus
US7276579B2 (en) 2000-12-27 2007-10-02 Dana-Farber Cancer Institute, Inc. Immunogenic proteoliposomes, and uses thereof
US7211659B2 (en) 2001-07-05 2007-05-01 Chiron Corporation Polynucleotides encoding antigenic HIV type C polypeptides, polypeptides and uses thereof
US7811577B2 (en) 2004-06-01 2010-10-12 Merck Sharp & Dohme Corp. Covalently stabilized chimeric coiled-coil HIV gp41 N-peptides with improved antiviral activity
ES2324755B1 (es) * 2007-04-04 2010-05-31 Consejo Superior De Investigaciones Cientificas (Csic) Polipeptido de la proteina gp120 capaz de inhibir el ciclo vital del virus del sida.
US9217011B2 (en) 2007-09-07 2015-12-22 Complix Nv Non-natural proteinaceous scaffold made of three non-covalently associated peptides
EP2161278B1 (fr) * 2008-09-08 2012-05-23 Complix N.V. Échafaudage à bobine à une seule chaîne
US10851133B2 (en) 2015-06-08 2020-12-01 New York University Stabilized minimal coiled-coil mimetics
US11891422B2 (en) 2018-11-16 2024-02-06 New York University NEMO coiled coil mimics and methods of using same
CN110184298B (zh) * 2019-05-15 2023-05-16 武汉璟泓科技股份有限公司 Hiv突变型表面糖蛋白及其纳米化抗原与制备方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4704694A (en) 1985-12-16 1987-11-03 Automation Intelligence, Inc. Learned part system
US4976778A (en) 1988-03-08 1990-12-11 Scm Metal Products, Inc. Infiltrated powder metal part and method for making same
EP0666923A1 (fr) 1991-09-05 1995-08-16 The University Of Connecticut Apport cible de polynucleotides ou d'oligonucleotides a des cellules
EP0745134A1 (fr) 1994-02-22 1996-12-04 Danafarber Cancer Institute Systeme de liberation d'acide nucleique, son procede de synthese et ses utilisations

Also Published As

Publication number Publication date
WO1999016883A2 (fr) 1999-04-08
CA2305341A1 (fr) 1999-04-08
AU9678598A (en) 1999-04-23
EP1019511A2 (fr) 2000-07-19
WO1999016883A3 (fr) 1999-06-17

Similar Documents

Publication Publication Date Title
US7429653B2 (en) Stabilized soluble glycoprotein trimers
US6716429B1 (en) Stabilization of envelope glycoprotein trimers by disulfide bonds introduced into a gp 41 glycoprotein ectodomain
Dowbenko et al. Epitope mapping of the human immunodeficiency virus type 1 gp120 with monoclonal antibodies
Xiang et al. A V3 loop-dependent gp120 element disrupted by CD4 binding stabilizes the human immunodeficiency virus envelope glycoprotein trimer
US7048929B1 (en) Stabilized primate lentivirus envelope glycoproteins
CA2305341C (fr) Stabilisation des glycoproteines trimeres d'enveloppe au moyen de liaisons bisulfure introduites dans un ectodomaine de la glycoproteine gp41
US7700115B2 (en) Therapeutic peptides and vaccines
EP1466924B9 (fr) Vaccins de peptide synthétiques pour HIV: l'épitope de CBD comme immunogen efficace pour obtenir des anticorps neutralisants contre VIH
JP2004522401A (ja) ワクチンおよび治療薬としてのｃｄ４非依存性のｈｉｖエンベロープタンパク質
US6908617B1 (en) Glycosylated modified primate lentivirus envelope polypeptides
Savarino et al. Human CD38 interferes with HIV‐1 fusion through a sequence homologous to the V3 loop of the viral envelope glycoprotein gp120.
EP0762895B1 (fr) Vaccin de synthese protegeant contre l'infection par le vih
EP1104310B1 (fr) Expression et caracterisation d'une proteine d'enveloppe vih-1 associee a une reponse d'anticorps de neutralisation largement reactifs
WO1997014436A9 (fr) Vaccin de synthese pour la protection contre l'infection par le virus de l'immunodeficience humaine
AU731975B2 (en) GP120 polypeptides having conformational discontinuous chemokine receptor binding sites and methods of inhibiting HIV infection
WO1999019501A1 (fr) Vecteur d'origine retrovirale d'adn non integrant a forte expression de proteines et antigenes immunogenes secretes
WO1998041536A1 (fr) Glycoproteines enveloppes de vih et vis a glycosylation deficiente
WO1998041536A9 (fr) Glycoproteines enveloppes de vih et vis a glycosylation deficiente
US7608688B2 (en) HIV-1 protein associated with broadly reactive neutralizing antibody response
WO1998015569A9 (fr) Polypeptides de gp120 possedant des sites de liaison aux recepteurs de la chimiokine a discontinuite de conformation et procedes d'inhibition des infections a vih
SODROSKI et al. Sommaire du brevet 2305341
SODROSKI et al. Patent 2305341 Summary
Barrett et al. Vaccines in AIDS: present state of knowledge
Wang et al. Three candidate peptide-vaccines in combination to induce high levels of multiantibodies against HIV-1
Woof et al. Antonis Mirsaliotis, Kulpash Nurkiyanova, Daniel Lamb

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed