CA2296093A1 - Proteines multifonctionnelles hybrides et recepteurs de lepidopterane modifies destines a etre utilises pour reguler l'expression transgenique - Google Patents
Proteines multifonctionnelles hybrides et recepteurs de lepidopterane modifies destines a etre utilises pour reguler l'expression transgenique Download PDFInfo
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
- C07K14/721—Steroid/thyroid hormone superfamily, e.g. GR, EcR, androgen receptor, oestrogen receptor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2799/00—Uses of viruses
- C12N2799/02—Uses of viruses as vector
- C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
- C12N2799/027—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a retrovirus
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Toxicology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Endocrinology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Selon la présente invention, il a été découvert que des protéines réceptrices nucléaires isolées et issues du ver à soie bombyx mori (bR) sont utilisées pour réguler l'expression transgénique. Le bR est généralement censé être un régulateur de transcription efficace à l'intérieur des cellules dudit ver à soie. Selon la présente invention, il a été découvert que le bR est également fonctionnel dans des cellules de mammifères, que l'ajout de domaines activateurs au cadre ouvert de lecture du bR stimule l'activité du régulateur modulé par des ligands et ce, dans le but d'obtenir une induction de transcription de haut niveau. Enfin, l'invention concerne d'autres modifications du domaine de liaison aux ligands du bR qui permettent d'obtenir des récepteurs présentant des propriétés de transactivation uniques.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/891,298 | 1997-07-10 | ||
| US08/891,298 US6300488B1 (en) | 1997-07-10 | 1997-07-10 | Modified lepidopteran receptors and hybrid multifunctional proteins for use in transcription and regulation of transgene expression |
| US9187498P | 1998-07-07 | 1998-07-07 | |
| US60/091,874 | 1998-07-07 | ||
| PCT/US1998/014215 WO1999002683A1 (fr) | 1997-07-10 | 1998-07-10 | Proteines multifonctionnelles hybrides et recepteurs de lepidopterane modifies destines a etre utilises pour reguler l'expression transgenique |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2296093A1 true CA2296093A1 (fr) | 1999-01-21 |
Family
ID=26784430
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002296093A Abandoned CA2296093A1 (fr) | 1997-07-10 | 1998-07-10 | Proteines multifonctionnelles hybrides et recepteurs de lepidopterane modifies destines a etre utilises pour reguler l'expression transgenique |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP0998560A1 (fr) |
| AU (1) | AU738494B2 (fr) |
| CA (1) | CA2296093A1 (fr) |
| WO (1) | WO1999002683A1 (fr) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000015791A1 (fr) | 1998-09-10 | 2000-03-23 | Pioneer Hi-Bred International, Inc. | Recepteurs de l'ecdysone et procedes d'utilisation desdits recepteurs |
| GB9915126D0 (en) * | 1999-06-30 | 1999-09-01 | Imp College Innovations Ltd | Control of gene expression |
| AU3642601A (en) * | 1999-11-05 | 2001-05-30 | Avigen, Inc. | Ecdysone-inducible adeno-associated virus expression vectors |
| WO2001062780A1 (fr) * | 2000-02-24 | 2001-08-30 | The Salk Institute For Biological Studies | Systeme d'expression genique a base de recepteurs chimeres |
| CN1304578C (zh) * | 2000-03-22 | 2007-03-14 | 罗姆和哈斯公司 | 新的基于蜕皮激素受体的可诱导的基因表达系统 |
| US20040033600A1 (en) | 2001-03-21 | 2004-02-19 | Palli Subba Reddy | Ecdysone receptor-based inducible gene expression system |
| US8105825B2 (en) | 2000-10-03 | 2012-01-31 | Intrexon Corporation | Multiple inducible gene regulation system |
| EP1356066A2 (fr) * | 2000-10-24 | 2003-10-29 | Syngenta Participations AG | Controle de l'expression genique dans des plantes |
| US20030049689A1 (en) * | 2000-12-14 | 2003-03-13 | Cynthia Edwards | Multifunctional polypeptides |
| EP1373470B1 (fr) | 2001-02-20 | 2013-04-24 | Intrexon Corporation | Nouveaux recepteurs de mutants de substitution et utilisation de ceux-ci dans un systeme d'expression genique inductible a base de recepteurs nucleaires |
| JP4955904B2 (ja) | 2001-02-20 | 2012-06-20 | イントレキソン コーポレーション | 新規エクジソン受容体／無脊椎動物レチノイドｘ受容体−ベースの誘導性遺伝子発現システム |
| ES2390070T3 (es) | 2001-02-20 | 2012-11-06 | Intrexon Corporation | Nuevos receptores mutantes de sustitución y su uso en un sistema de expresión génica inducible basado en receptores nucleares |
| CA2438119C (fr) * | 2001-02-20 | 2014-12-16 | Rheogene Holdings, Inc. | Recepteurs x de retinoides chimeriques et utilisations de ceux-ci dans un nouveau systeme d'expression de gene inductible fonde sur un recepteur d'ecdysone |
| MXPA04002808A (es) | 2001-09-26 | 2005-06-06 | Rheogene Holdings Inc | Acidos nucleicos receptores de ecdisona de mosca blanca, polipeptidos, y sus usos. |
| US7563879B2 (en) | 2001-09-26 | 2009-07-21 | Intrexon Corporation | Leafhopper ecdysone receptor nucleic acids, polypeptides, and uses thereof |
| US7375093B2 (en) | 2002-07-05 | 2008-05-20 | Intrexon Corporation | Ketone ligands for modulating the expression of exogenous genes via an ecdysone receptor complex |
| US7304161B2 (en) | 2003-02-10 | 2007-12-04 | Intrexon Corporation | Diaclhydrazine ligands for modulating the expression of exogenous genes in mammalian systems via an ecdysone receptor complex |
| US7456315B2 (en) | 2003-02-28 | 2008-11-25 | Intrexon Corporation | Bioavailable diacylhydrazine ligands for modulating the expression of exogenous genes via an ecdysone receptor complex |
| US7935510B2 (en) * | 2004-04-30 | 2011-05-03 | Intrexon Corporation | Mutant receptors and their use in a nuclear receptor-based inducible gene expression system |
| CA2689137C (fr) | 2007-05-29 | 2017-05-02 | Intrexon Corporation | Ligands diacylhydrazine chiraux destines a moduler l'expression de genes exogenes par le biais d'un complexe recepteur de l'ecdysone |
| US8536354B2 (en) | 2008-03-14 | 2013-09-17 | Intrexon Corporation | Steroidal ligands and their use in gene switch modulation |
| BR112015022994A2 (pt) | 2013-03-15 | 2017-11-14 | Intrexon Corp | diacilhidrazinas contendo boro |
| AU2015317862A1 (en) | 2014-09-17 | 2017-04-06 | Intrexon Corporation | Boron-containing diacylhydrazine compounds |
| CA3049652A1 (fr) | 2017-01-10 | 2018-07-19 | Intrexon Corporation | Modulation de l'expression de polypeptides par l'intermediaire de nouveaux systemes d'expression de commutateurs geniques |
| KR20230115343A (ko) | 2017-06-07 | 2023-08-02 | 프레시전 인코포레이티드 | 신규의 세포 태그의 발현 |
| CN112135622A (zh) | 2018-03-06 | 2020-12-25 | 普莱西根股份有限公司 | 乙型肝炎疫苗及其用途 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX9709156A (es) * | 1995-05-26 | 1998-03-31 | Zeneca Ltd | Un interruptor de gen que comprende un receptor ecdisona. |
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1998
- 1998-07-10 AU AU83895/98A patent/AU738494B2/en not_active Ceased
- 1998-07-10 EP EP98934353A patent/EP0998560A1/fr not_active Withdrawn
- 1998-07-10 WO PCT/US1998/014215 patent/WO1999002683A1/fr not_active Application Discontinuation
- 1998-07-10 CA CA002296093A patent/CA2296093A1/fr not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP0998560A1 (fr) | 2000-05-10 |
| AU8389598A (en) | 1999-02-08 |
| AU738494B2 (en) | 2001-09-20 |
| WO1999002683A1 (fr) | 1999-01-21 |
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