CA2294619A1 - Procedes de preformage de recombinaison homologue d'apres une modification d'acides nucleiques dans des cellules presentant une deficience de recombinaison, et utilisation des produits d'acides nucleiques modifies de celles-ci - Google Patents

Procedes de preformage de recombinaison homologue d'apres une modification d'acides nucleiques dans des cellules presentant une deficience de recombinaison, et utilisation des produits d'acides nucleiques modifies de celles-ci Download PDF

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Publication number
CA2294619A1
CA2294619A1 CA002294619A CA2294619A CA2294619A1 CA 2294619 A1 CA2294619 A1 CA 2294619A1 CA 002294619 A CA002294619 A CA 002294619A CA 2294619 A CA2294619 A CA 2294619A CA 2294619 A1 CA2294619 A1 CA 2294619A1
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CA
Canada
Prior art keywords
reca
gene
protein
host cell
tssv
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002294619A
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English (en)
Inventor
Nathaniel Heintz
Peter Model
Xiangdong W. Yang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rockefeller University
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/880,966 external-priority patent/US6143566A/en
Application filed by Individual filed Critical Individual
Publication of CA2294619A1 publication Critical patent/CA2294619A1/fr
Abandoned legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/20—Pseudochromosomes, minichrosomosomes
    • C12N2800/202—Pseudochromosomes, minichrosomosomes of bacteriophage origin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/20—Pseudochromosomes, minichrosomosomes
    • C12N2800/204—Pseudochromosomes, minichrosomosomes of bacterial origin, e.g. BAC
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Environmental Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne un procédé simple destiné à modifier des gènes dans une cellule hôte présentant une déficience de recombinaison. De telles modifications consistent à produire une insertion, des délétions, des substitutions et/ou des mutations ponctuelles au niveau d'un quelconque site choisi dans le vecteur de clonage d'origine indépendante. Le gène modifié peut être contenu dans ce vecteur de clonage d'origine indépendante que l'on utilise pour introduire un gène hétérologue modifié dans une cellule. On peut employer un tel vecteur modifié dans la production d'un animal transgénique obtenu par lignée germinale, ou dans des protocoles de ciblage de gène dans des cellules eucaryotes.
CA002294619A 1997-06-23 1998-06-23 Procedes de preformage de recombinaison homologue d'apres une modification d'acides nucleiques dans des cellules presentant une deficience de recombinaison, et utilisation des produits d'acides nucleiques modifies de celles-ci Abandoned CA2294619A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US08/880,966 US6143566A (en) 1997-06-23 1997-06-23 Methods of performing homologous recombination based modification of nucleic acids in recombination deficient cells and use of the modified nucleic acid products thereof
US08/880,966 1997-06-23
US10249098A 1998-06-22 1998-06-22
US09/102,490 1998-06-22
PCT/US1998/012966 WO1998059060A1 (fr) 1997-06-23 1998-06-23 Procedes de preformage de recombinaison homologue d'apres une modification d'acides nucleiques dans des cellules presentant une deficience de recombinaison, et utilisation des produits d'acides nucleiques modifies de celles-ci

Publications (1)

Publication Number Publication Date
CA2294619A1 true CA2294619A1 (fr) 1998-12-30

Family

ID=26799422

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002294619A Abandoned CA2294619A1 (fr) 1997-06-23 1998-06-23 Procedes de preformage de recombinaison homologue d'apres une modification d'acides nucleiques dans des cellules presentant une deficience de recombinaison, et utilisation des produits d'acides nucleiques modifies de celles-ci

Country Status (5)

Country Link
EP (1) EP0998574A1 (fr)
JP (1) JP2002515764A (fr)
AU (1) AU730859B2 (fr)
CA (1) CA2294619A1 (fr)
WO (1) WO1998059060A1 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6821759B1 (en) 1997-06-23 2004-11-23 The Rockefeller University Methods of performing homologous recombination based modification of nucleic acids in recombination deficient cells and use of the modified nucleic acid products thereof
US6156574A (en) 1997-06-23 2000-12-05 The Rockefeller University Methods of performing gene trapping in bacterial and bacteriophage-derived artificial chromosomes and use thereof
US6355412B1 (en) * 1999-07-09 2002-03-12 The European Molecular Biology Laboratory Methods and compositions for directed cloning and subcloning using homologous recombination
WO2001005962A1 (fr) * 1999-07-20 2001-01-25 The Rockefeller University Recombinaison homologue conditionnelle de grands segments d'insertion de vecteur genomiques
AU2001270162A1 (en) * 2000-06-29 2002-01-14 The Rockefeller University Activation of gene expression in cloned eukaryotic genomic dna and methods of use thereof
JP5670755B2 (ja) 2008-03-12 2015-02-18 ザ ロックフェラー ユニバーシティ 翻訳プロファイリング及び分子表現型解析のための方法及び組成物

Also Published As

Publication number Publication date
EP0998574A1 (fr) 2000-05-10
AU730859B2 (en) 2001-03-15
JP2002515764A (ja) 2002-05-28
AU7984898A (en) 1999-01-04
WO1998059060A1 (fr) 1998-12-30

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Legal Events

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FZDE Discontinued