CA2293737A1 - Souches de levure transformees et leur utilisation dans la production de carboxylates aliphatiques a terminaison unique et a terminaison double - Google Patents

Souches de levure transformees et leur utilisation dans la production de carboxylates aliphatiques a terminaison unique et a terminaison double Download PDF

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Publication number
CA2293737A1
CA2293737A1 CA002293737A CA2293737A CA2293737A1 CA 2293737 A1 CA2293737 A1 CA 2293737A1 CA 002293737 A CA002293737 A CA 002293737A CA 2293737 A CA2293737 A CA 2293737A CA 2293737 A1 CA2293737 A1 CA 2293737A1
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CA
Canada
Prior art keywords
candida maltosa
cytochrome
gene
reductase
stably transformed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002293737A
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English (en)
Inventor
Robert D. Fallon
Mark S. Payne
Stephen K. Picataggio
Shijun Wu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2293737A1 publication Critical patent/CA2293737A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12N9/0042—NADPH-cytochrome P450 reductase (1.6.2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/165—Yeast isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/72—Candida
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/84—Pichia

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La présente invention concerne un processus biologique permettant de transformer des composés aliphatiques de la forme CH¿3?(CH¿2?)¿n?CH¿3?, dans laquelle n = 4 à 20, en carboxylates à terminaison unique et à terminaison double à l'aide d'organismes génétiquement modifiés. L'invention se rapporte à un processus permettant l'expression d'une activité d'hydroxylation d'alcane dans des levures Pichia pastoris et Candida maltosa génétiquement modifiées. En outre, l'invention concerne un processus permettant de produire des souches de Candida maltosa génétiquement modifiées déployant une activité de cytochrome P-450 améliorée et/ou des disruptions géniques de la voie d'oxydation .beta..
CA002293737A 1997-07-21 1998-07-20 Souches de levure transformees et leur utilisation dans la production de carboxylates aliphatiques a terminaison unique et a terminaison double Abandoned CA2293737A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US5321597P 1997-07-21 1997-07-21
US60/053,215 1997-07-21
PCT/US1998/014935 WO1999004014A2 (fr) 1997-07-21 1998-07-20 Souches de levure transformees et leur utilisation dans la production de carboxylates aliphatiques a terminaison unique et a terminaison double

Publications (1)

Publication Number Publication Date
CA2293737A1 true CA2293737A1 (fr) 1999-01-28

Family

ID=21982693

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002293737A Abandoned CA2293737A1 (fr) 1997-07-21 1998-07-20 Souches de levure transformees et leur utilisation dans la production de carboxylates aliphatiques a terminaison unique et a terminaison double

Country Status (6)

Country Link
EP (1) EP1003881A2 (fr)
JP (1) JP2001510048A (fr)
AU (1) AU8498298A (fr)
CA (1) CA2293737A1 (fr)
IL (1) IL133931A0 (fr)
WO (1) WO1999004014A2 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000065061A2 (fr) * 1999-04-24 2000-11-02 Max-Delbrück-Centrum für Molekulare Medizin Sequences d'acide nucleique issues de levures de candida, codant des polypeptides b5 cytochromes
DE10036491A1 (de) * 2000-07-25 2002-02-07 Roche Diagnostics Gmbh Expression von Alkalischer Phosphatase in Hefe
KR20110101198A (ko) * 2008-12-12 2011-09-15 이 아이 듀폰 디 네모아 앤드 캄파니 재생 가능 자원으로부터의 선형 다이카르복실산의 제조 방법
DE102010015807A1 (de) * 2010-04-20 2011-10-20 Evonik Degussa Gmbh Biokatalytisches Oxidationsverfahren mit alkL-Genprodukt
US8728798B2 (en) 2011-05-03 2014-05-20 Verdezyne, Inc. Biological methods for preparing adipic acid
WO2013006733A2 (fr) * 2011-07-06 2013-01-10 Verdezyne, Inc. Procédés biologiques pour la préparation d'acide gras dicarboxylique
EP3173478A1 (fr) * 2015-11-25 2017-05-31 Evonik Degussa GmbH Production biotechnologique d'acides carboxyliques fonctionalisés omega et esters associés
IT201700091114A1 (it) * 2017-08-09 2019-02-09 Novamont Spa Candida geneticamente modificata per la produzione di acidi grassi dicarbossilici
CN110684809A (zh) * 2018-07-06 2020-01-14 上海凯赛生物技术股份有限公司 一种发酵法生产的十二碳二元酸产品及其制备方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5019630B1 (fr) * 1969-11-10 1975-07-08
DE3721119A1 (de) * 1987-06-26 1989-01-05 Henkel Kgaa Fermentative herstellung von dicarbonsaeuren
US5254466A (en) * 1989-11-06 1993-10-19 Henkel Research Corporation Site-specific modification of the candida tropicals genome
DE19507546C2 (de) * 1995-03-03 2001-05-03 Max Delbrueck Centrum Verfahren zur regioselektiven Hydroxylierung von langkettigen Alkanen, Fettsäuren und anderen Alkylverbindungen

Also Published As

Publication number Publication date
JP2001510048A (ja) 2001-07-31
WO1999004014A3 (fr) 1999-05-20
WO1999004014A2 (fr) 1999-01-28
EP1003881A2 (fr) 2000-05-31
IL133931A0 (en) 2001-04-30
AU8498298A (en) 1999-02-10

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Legal Events

Date Code Title Description
FZDE Discontinued