CA2288030A1 - Combinatorial polyketide libraries produced using a modular pks gene cluster as scaffold - Google Patents
Combinatorial polyketide libraries produced using a modular pks gene cluster as scaffold Download PDFInfo
- Publication number
- CA2288030A1 CA2288030A1 CA002288030A CA2288030A CA2288030A1 CA 2288030 A1 CA2288030 A1 CA 2288030A1 CA 002288030 A CA002288030 A CA 002288030A CA 2288030 A CA2288030 A CA 2288030A CA 2288030 A1 CA2288030 A1 CA 2288030A1
- Authority
- CA
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- Prior art keywords
- pks
- library
- alkyl
- compound
- colonies
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
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- 125000000335 thiazolyl group Chemical group 0.000 description 1
- 238000007079 thiolysis reaction Methods 0.000 description 1
- NSFFHOGKXHRQEW-AIHSUZKVSA-N thiostrepton Chemical compound C([C@]12C=3SC=C(N=3)C(=O)N[C@H](C(=O)NC(/C=3SC[C@@H](N=3)C(=O)N[C@H](C=3SC=C(N=3)C(=O)N[C@H](C=3SC=C(N=3)[C@H]1N=1)[C@@H](C)OC(=O)C3=CC(=C4C=C[C@H]([C@@H](C4=N3)O)N[C@H](C(N[C@@H](C)C(=O)NC(=C)C(=O)N[C@@H](C)C(=O)N2)=O)[C@@H](C)CC)[C@H](C)O)[C@](C)(O)[C@@H](C)O)=C\C)[C@@H](C)O)CC=1C1=NC(C(=O)NC(=C)C(=O)NC(=C)C(N)=O)=CS1 NSFFHOGKXHRQEW-AIHSUZKVSA-N 0.000 description 1
- 229940063214 thiostrepton Drugs 0.000 description 1
- 229930188070 thiostrepton Natural products 0.000 description 1
- NSFFHOGKXHRQEW-OFMUQYBVSA-N thiostrepton A Natural products CC[C@H](C)[C@@H]1N[C@@H]2C=Cc3c(cc(nc3[C@H]2O)C(=O)O[C@H](C)[C@@H]4NC(=O)c5csc(n5)[C@@H](NC(=O)[C@H]6CSC(=N6)C(=CC)NC(=O)[C@@H](NC(=O)c7csc(n7)[C@]8(CCC(=N[C@@H]8c9csc4n9)c%10nc(cs%10)C(=O)NC(=C)C(=O)NC(=C)C(=O)N)NC(=O)[C@H](C)NC(=O)C(=C)NC(=O)[C@H](C)NC1=O)[C@@H](C)O)[C@](C)(O)[C@@H](C)O)[C@H](C)O NSFFHOGKXHRQEW-OFMUQYBVSA-N 0.000 description 1
- HPGGPRDJHPYFRM-UHFFFAOYSA-J tin(iv) chloride Chemical compound Cl[Sn](Cl)(Cl)Cl HPGGPRDJHPYFRM-UHFFFAOYSA-J 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
- 238000003828 vacuum filtration Methods 0.000 description 1
- 239000003039 volatile agent Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- DFKDOZMCHOGOBR-NCSQYGPNSA-N zaragozic acid A Chemical compound C([C@@H](C)[C@H](OC(C)=O)C(=C)CC[C@]12[C@H](O)[C@H]([C@](O2)(C(O)=O)[C@@](O)([C@H](O1)C(O)=O)C(O)=O)OC(=O)/C=C/[C@@H](C)C[C@@H](C)CC)C1=CC=CC=C1 DFKDOZMCHOGOBR-NCSQYGPNSA-N 0.000 description 1
- DFKDOZMCHOGOBR-UHFFFAOYSA-N zaragozic acid A Natural products O1C(C(O)(C(O2)C(O)=O)C(O)=O)(C(O)=O)C(OC(=O)C=CC(C)CC(C)CC)C(O)C21CCC(=C)C(OC(C)=O)C(C)CC1=CC=CC=C1 DFKDOZMCHOGOBR-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H17/00—Compounds containing heterocyclic radicals directly attached to hetero atoms of saccharide radicals
- C07H17/04—Heterocyclic radicals containing only oxygen as ring hetero atoms
- C07H17/08—Hetero rings containing eight or more ring members, e.g. erythromycins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D323/00—Heterocyclic compounds containing more than two oxygen atoms as the only ring hetero atoms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oncology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Veterinary Medicine (AREA)
- Plant Pathology (AREA)
- Public Health (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Communicable Diseases (AREA)
- Pharmacology & Pharmacy (AREA)
- Saccharide Compounds (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Pyrane Compounds (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/846,247 | 1997-04-30 | ||
| US08/846,247 US6391594B1 (en) | 1993-09-20 | 1997-04-30 | Modified modular PKS with retained scaffold |
| US7691998P | 1998-03-05 | 1998-03-05 | |
| US60/076,919 | 1998-03-05 | ||
| PCT/US1998/008792 WO1998049315A2 (en) | 1997-04-30 | 1998-04-30 | Combinatorial polyketide libraries produced using a modular pks gene cluster as scaffold |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2288030A1 true CA2288030A1 (en) | 1998-11-05 |
Family
ID=26758639
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002288030A Abandoned CA2288030A1 (en) | 1997-04-30 | 1998-04-30 | Combinatorial polyketide libraries produced using a modular pks gene cluster as scaffold |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP0979286B1 (enExample) |
| JP (1) | JP2001524829A (enExample) |
| AU (1) | AU732909B2 (enExample) |
| CA (1) | CA2288030A1 (enExample) |
| IL (1) | IL132590A0 (enExample) |
| NZ (1) | NZ500693A (enExample) |
| WO (1) | WO1998049315A2 (enExample) |
Families Citing this family (46)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6063561A (en) * | 1991-01-17 | 2000-05-16 | Abbott Laboratories | Polyketide derivatives and recombinant methods for making same |
| US6066721A (en) * | 1995-07-06 | 2000-05-23 | Stanford University | Method to produce novel polyketides |
| US6495348B1 (en) | 1993-10-07 | 2002-12-17 | Regents Of The University Of Minnesota | Mitomycin biosynthetic gene cluster |
| US6399789B1 (en) | 1996-12-18 | 2002-06-04 | Kosan Biosciences, Inc. | Multi-plasmid method for preparing large libraries of polyketides and non-ribosomal peptides |
| GB9814006D0 (en) * | 1998-06-29 | 1998-08-26 | Biotica Tech Ltd | Polyketides and their synthesis |
| JP2002525026A (ja) | 1998-07-02 | 2002-08-13 | ザ ボード オブ リージェンツ オブ ザ レランド スタンフォード ジュニア ユニバーシティ | ポリケチドを作製するための方法 |
| CA2343880C (en) * | 1998-10-02 | 2008-08-26 | Kosan Biosciences, Inc. | Polyketide synthase enzymes and recombinant dna constructs therefor |
| JP2002528467A (ja) * | 1998-10-28 | 2002-09-03 | コーサン バイオサイエンシーズ， インコーポレイテッド | 新規な「不自然な（ｕｎｎａｔｕｒａｌ）」天然生成物のライブラリ |
| EP1135470A2 (en) | 1998-11-20 | 2001-09-26 | Kosan Biosciences, Inc. | Recombinant methods and materials for producing epothilone and epothilone derivatives |
| US6410301B1 (en) | 1998-11-20 | 2002-06-25 | Kosan Biosciences, Inc. | Myxococcus host cells for the production of epothilones |
| US6358712B1 (en) | 1999-01-05 | 2002-03-19 | Trustee Of Boston University | Ordered gene assembly |
| EP1141275B1 (en) | 1999-01-05 | 2009-08-12 | Trustees Of Boston University | Improved nucleic acid cloning |
| WO2000044717A2 (en) * | 1999-01-27 | 2000-08-03 | Kosan Biosciences, Inc. | Synthesis of oligoketides |
| EP1754700A3 (en) * | 1999-01-27 | 2007-05-09 | Kosan Biosciences, Inc. | Synthesis of oligoketides |
| CA2359801A1 (en) * | 1999-02-09 | 2000-08-17 | Rajesh S. Gokhale | Methods to mediate polyketide synthase module effectiveness |
| US7001748B2 (en) | 1999-02-09 | 2006-02-21 | The Board Of Trustees Of The Leland Stanford Junior University | Methods of making polyketides using hybrid polyketide synthases |
| US6451768B1 (en) | 1999-04-16 | 2002-09-17 | Kosan Biosciences, Inc. | Macrolide antiinfective agents |
| CN1373767A (zh) * | 1999-04-16 | 2002-10-09 | 奥索-麦克尼尔药品公司 | 酮式大环内酯抗菌剂 |
| DE60028956T2 (de) * | 1999-04-16 | 2006-11-30 | Kosan Biosciences, Inc., Hayward | Multi-plasmid verfahren zur herstellung von grossen bibliotheken von polyketiden und nicht-ribosomalen peptiden |
| US6590083B1 (en) | 1999-04-16 | 2003-07-08 | Ortho-Mcneil Pharmaceutical, Inc. | Ketolide antibacterials |
| GB9912563D0 (en) * | 1999-05-28 | 1999-07-28 | Biotica Tech Ltd | Polyketides and their synthesis |
| GB9913694D0 (en) * | 1999-06-11 | 1999-08-11 | Simper Adrian M | DNA manipulation methods and applications for synthetic enzymes |
| US6569867B2 (en) | 1999-10-01 | 2003-05-27 | Kosan Biosciences, Inc. | Polyketide derivatives |
| US7033818B2 (en) | 1999-10-08 | 2006-04-25 | Kosan Biosciences, Inc. | Recombinant polyketide synthase genes |
| US6524841B1 (en) | 1999-10-08 | 2003-02-25 | Kosan Biosciences, Inc. | Recombinant megalomicin biosynthetic genes and uses thereof |
| DE60026563D1 (de) | 1999-10-25 | 2006-05-04 | Kosan Biosciences Inc | Herstellung von polyketiden |
| MXPA02004153A (es) | 1999-10-27 | 2002-10-17 | Kosan Biosciences Inc | Produccion heterologa de policetidos. |
| ATE356880T1 (de) | 1999-10-29 | 2007-04-15 | Kosan Biosciences Inc | Rapamycin analoge |
| WO2001059126A2 (en) | 2000-02-08 | 2001-08-16 | Norges Teknisk Naturvitenskapelige Universitet | Gene cluster encoding a nystatin polyketide synthase and its manipulation and utility |
| WO2001079520A1 (en) * | 2000-04-13 | 2001-10-25 | Biotica Technology Limited | Hybrid glycosylated products and their production and use |
| US6838265B2 (en) | 2000-05-02 | 2005-01-04 | Kosan Biosciences, Inc. | Overproduction hosts for biosynthesis of polyketides |
| AU2001275012A1 (en) | 2000-05-30 | 2001-12-11 | Kosan Biosciences, Inc. | Design of polyketide synthase genes |
| WO2002099113A1 (en) * | 2001-06-01 | 2002-12-12 | Ajinomoto Co., Inc. | Macrolide compounds, antifungal agents using the same, macrolide compound-producing bacterium belonging to the genus sorangium and process for producing macrolide compounds using the same |
| US7189549B2 (en) * | 2002-06-14 | 2007-03-13 | Kosan Biosciences, Inc. | Recombinant polynucleotides encoding pro-geldanamycin producing polyketide synthase and accessory proteins, and uses thereof |
| US7427493B2 (en) | 2002-06-28 | 2008-09-23 | Kosan Biosciences Incorporated | Recombinant genes for polyketide modifying enzymes |
| KR100549690B1 (ko) | 2003-10-23 | 2006-02-07 | 상하이 지아오통 대학 | 에프알-008 폴리케타이드 합성에 관여하는 유전자 |
| EP1751279A4 (en) | 2004-05-05 | 2008-03-19 | Univ Minnesota | NUCLEIC ACIDS AND POLYPEPTIDES INVOLVED IN THE PREPARATION OF CRYPTOPHYCIN |
| US20050272166A1 (en) * | 2004-05-06 | 2005-12-08 | Li Jin | Methods and systems for detection, identification and quantitation of macrolides and their impurities |
| US7566558B2 (en) | 2006-07-28 | 2009-07-28 | Regents Of The University Of Michigan | Nucleic acids and polypeptides involved in the production of cryptophycin |
| US8759031B2 (en) | 2007-04-12 | 2014-06-24 | Wisconsin Alumni Research Foundation | Type I polyketide synthase extender units |
| WO2009121066A1 (en) | 2008-03-28 | 2009-10-01 | The Regents Of The University Of California | Producing dicarboxylic acids using polyketide synthases |
| CN102014623A (zh) * | 2008-04-29 | 2011-04-13 | 加利福尼亚大学董事会 | 使用聚酮化物合酶制备生物燃料 |
| US8852902B2 (en) | 2010-11-22 | 2014-10-07 | The Regents Of The University Of California | Producing a trimethylpentanoic acid using hybrid polyketide synthases |
| GB201312318D0 (en) * | 2013-07-09 | 2013-08-21 | Isomerase Therapeutics Ltd | Novel methods and compounds |
| US10233431B2 (en) | 2014-02-26 | 2019-03-19 | The Regents Of The University Of California | Producing 3-hydroxycarboxylic acid and ketone using polyketide synthases |
| CN112301005B (zh) * | 2020-11-09 | 2023-01-24 | 浙江大学 | 一种6-脱氧赤酮酸内酯b合酶复合体3的制备方法 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5712146A (en) * | 1993-09-20 | 1998-01-27 | The Leland Stanford Junior University | Recombinant combinatorial genetic library for the production of novel polyketides |
| EP0910633B1 (en) | 1996-07-05 | 2009-12-09 | Biotica Technology Limited | Hybrid polyketide synthase I gene |
-
1998
- 1998-04-30 IL IL13259098A patent/IL132590A0/xx unknown
- 1998-04-30 AU AU71722/98A patent/AU732909B2/en not_active Ceased
- 1998-04-30 EP EP98918891.7A patent/EP0979286B1/en not_active Expired - Lifetime
- 1998-04-30 JP JP54741398A patent/JP2001524829A/ja not_active Withdrawn
- 1998-04-30 WO PCT/US1998/008792 patent/WO1998049315A2/en not_active Ceased
- 1998-04-30 NZ NZ500693A patent/NZ500693A/en active Application Revival
- 1998-04-30 CA CA002288030A patent/CA2288030A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO1998049315A3 (en) | 1999-01-14 |
| JP2001524829A (ja) | 2001-12-04 |
| AU7172298A (en) | 1998-11-24 |
| AU732909B2 (en) | 2001-05-03 |
| IL132590A0 (en) | 2001-03-19 |
| NZ500693A (en) | 2004-01-30 |
| EP0979286A2 (en) | 2000-02-16 |
| EP0979286B1 (en) | 2014-01-29 |
| WO1998049315A2 (en) | 1998-11-05 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Discontinued |