CA2284097A1 - Shuffling of heterologous dna sequences - Google Patents
Shuffling of heterologous dna sequences Download PDFInfo
- Publication number
- CA2284097A1 CA2284097A1 CA002284097A CA2284097A CA2284097A1 CA 2284097 A1 CA2284097 A1 CA 2284097A1 CA 002284097 A CA002284097 A CA 002284097A CA 2284097 A CA2284097 A CA 2284097A CA 2284097 A1 CA2284097 A1 CA 2284097A1
- Authority
- CA
- Canada
- Prior art keywords
- sequences
- conserved region
- pcr
- primer
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
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- VGONTNSXDCQUGY-RRKCRQDMSA-N 2'-deoxyinosine Chemical group C1[C@H](O)[C@@H](CO)O[C@H]1N1C(N=CNC2=O)=C2N=C1 VGONTNSXDCQUGY-RRKCRQDMSA-N 0.000 description 1
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- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 1
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- 108010019653 Pwo polymerase Proteins 0.000 description 1
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- 108090000787 Subtilisin Proteins 0.000 description 1
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- 108700005078 Synthetic Genes Proteins 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
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- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK30497 | 1997-03-18 | ||
| DK0304/97 | 1997-03-18 | ||
| DK0432/97 | 1997-04-17 | ||
| DK43297 | 1997-04-17 | ||
| PCT/DK1998/000105 WO1998041623A1 (en) | 1997-03-18 | 1998-03-18 | Shuffling of heterologous dna sequences |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2284097A1 true CA2284097A1 (en) | 1998-09-24 |
Family
ID=26063733
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002284097A Abandoned CA2284097A1 (en) | 1997-03-18 | 1998-03-18 | Shuffling of heterologous dna sequences |
Country Status (10)
| Country | Link |
|---|---|
| EP (1) | EP1015575B1 (https=) |
| JP (1) | JP2001516216A (https=) |
| AT (1) | ATE468394T1 (https=) |
| AU (1) | AU738461B2 (https=) |
| CA (1) | CA2284097A1 (https=) |
| DE (1) | DE69841674D1 (https=) |
| DK (1) | DK1015575T3 (https=) |
| IL (1) | IL131986A0 (https=) |
| NZ (1) | NZ337910A (https=) |
| WO (1) | WO1998041623A1 (https=) |
Families Citing this family (60)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6335160B1 (en) | 1995-02-17 | 2002-01-01 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
| US6995017B1 (en) | 1994-02-17 | 2006-02-07 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US6309883B1 (en) | 1994-02-17 | 2001-10-30 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
| US6117679A (en) | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US20060257890A1 (en) | 1996-05-20 | 2006-11-16 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
| US6936289B2 (en) | 1995-06-07 | 2005-08-30 | Danisco A/S | Method of improving the properties of a flour dough, a flour dough improving composition and improved food products |
| US7148054B2 (en) | 1997-01-17 | 2006-12-12 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
| US6326204B1 (en) | 1997-01-17 | 2001-12-04 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
| US6153410A (en) * | 1997-03-25 | 2000-11-28 | California Institute Of Technology | Recombination of polynucleotide sequences using random or defined primers |
| ES2168236T3 (es) | 1997-04-09 | 2005-04-16 | Danisco A/S | Utilizacion de lipasa para mejorar las pastas de pan y los productos de panaderia. |
| US6537746B2 (en) | 1997-12-08 | 2003-03-25 | Maxygen, Inc. | Method for creating polynucleotide and polypeptide sequences |
| US7390619B1 (en) | 1998-02-11 | 2008-06-24 | Maxygen, Inc. | Optimization of immunomodulatory properties of genetic vaccines |
| IL139093A0 (en) | 1998-05-01 | 2001-11-25 | Maxygen Inc | Optimization of pest resistance genes using dna shuffling |
| US7153655B2 (en) | 1998-06-16 | 2006-12-26 | Alligator Bioscience Ab | Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence |
| US6846655B1 (en) | 1998-06-29 | 2005-01-25 | Phylos, Inc. | Methods for generating highly diverse libraries |
| JP2002522072A (ja) | 1998-08-12 | 2002-07-23 | マキシジェン， インコーポレイテッド | 工業用化学薬品の製造のためのモノオキシゲナーゼ遺伝子のｄｎａシャッフリング。 |
| JP2002526107A (ja) | 1998-10-07 | 2002-08-20 | マキシジェン， インコーポレイテッド | マイコトキシンの解毒のための核酸を生成するためのｄｎａシャッフリング |
| WO2000028018A1 (en) | 1998-11-10 | 2000-05-18 | Maxygen, Inc. | Modified adp-glucose pyrophosphorylase for improvement and optimization of plant phenotypes |
| US6436675B1 (en) | 1999-09-28 | 2002-08-20 | Maxygen, Inc. | Use of codon-varied oligonucleotide synthesis for synthetic shuffling |
| US6917882B2 (en) | 1999-01-19 | 2005-07-12 | Maxygen, Inc. | Methods for making character strings, polynucleotides and polypeptides having desired characteristics |
| US6376246B1 (en) | 1999-02-05 | 2002-04-23 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
| US6961664B2 (en) | 1999-01-19 | 2005-11-01 | Maxygen | Methods of populating data structures for use in evolutionary simulations |
| ES2341217T3 (es) | 1999-01-19 | 2010-06-17 | Maxygen, Inc. | Recombinacion de acidos nucleicos mediada por oligonucleotidos. |
| US7024312B1 (en) | 1999-01-19 | 2006-04-04 | Maxygen, Inc. | Methods for making character strings, polynucleotides and polypeptides having desired characteristics |
| JP2003524394A (ja) | 1999-02-11 | 2003-08-19 | マキシジェン， インコーポレイテッド | ハイスループット質量分析法 |
| US6531316B1 (en) | 1999-03-05 | 2003-03-11 | Maxyag, Inc. | Encryption of traits using split gene sequences and engineered genetic elements |
| WO2000061731A2 (en) * | 1999-04-13 | 2000-10-19 | Maxygen, Inc. | Modified starch metabolism enzymes and encoding genes for improvement and optimization of plant phenotypes |
| US7430477B2 (en) | 1999-10-12 | 2008-09-30 | Maxygen, Inc. | Methods of populating data structures for use in evolutionary simulations |
| US6686515B1 (en) | 1999-11-23 | 2004-02-03 | Maxygen, Inc. | Homologous recombination in plants |
| US7115712B1 (en) | 1999-12-02 | 2006-10-03 | Maxygen, Inc. | Cytokine polypeptides |
| IL150291A0 (en) | 2000-01-11 | 2002-12-01 | Maxygen Inc | Integrated systems and methods for diversity generation and screening |
| US6248541B1 (en) | 2000-04-21 | 2001-06-19 | Genencor International, Inc. | Screening under nutrient limited conditions |
| US6534292B1 (en) | 2000-05-08 | 2003-03-18 | Genencor International, Inc. | Methods for forming recombined nucleic acids |
| EP1283877A2 (en) * | 2000-05-23 | 2003-02-19 | California Institute Of Technology | Gene recombination and hybrid protein development |
| EP1360290A2 (en) | 2000-06-23 | 2003-11-12 | Maxygen, Inc. | Co-stimulatory molecules |
| WO2002000897A2 (en) | 2000-06-23 | 2002-01-03 | Maxygen, Inc. | Novel chimeric promoters |
| EP1383881A2 (en) | 2000-06-26 | 2004-01-28 | Novozymes A/S | Lipolytic enzymes from strains of fusarium and acremonium |
| US6994963B1 (en) | 2000-07-10 | 2006-02-07 | Ambion, Inc. | Methods for recombinatorial nucleic acid synthesis |
| US6858422B2 (en) | 2000-07-13 | 2005-02-22 | Codexis, Inc. | Lipase genes |
| AUPQ974900A0 (en) * | 2000-08-29 | 2000-09-21 | Macquarie Research Limited | Degenerate oligonucleotide gene-shuffling |
| US6582914B1 (en) | 2000-10-26 | 2003-06-24 | Genencor International, Inc. | Method for generating a library of oligonucleotides comprising a controlled distribution of mutations |
| US6958213B2 (en) | 2000-12-12 | 2005-10-25 | Alligator Bioscience Ab | Method for in vitro molecular evolution of protein function |
| BR0209154A (pt) | 2001-05-18 | 2004-07-20 | Danisco | Processo de preparação de uma massa com uma enzima |
| DK2295544T3 (en) | 2001-06-26 | 2016-09-26 | Novozymes As | Polypeptides with cellobiohydrolase I activity and the same coding polynucleotides |
| US7402383B2 (en) | 2001-07-23 | 2008-07-22 | Dsm Ip Assets B.V. | Process for preparing variant polynucleotides |
| DK1504098T4 (da) | 2002-05-17 | 2011-05-23 | Alligator Bioscience Ab | Fremgangsmåde til molekyulær udvikling in vitro af proteinfunktion |
| EP1578963B1 (en) | 2002-12-20 | 2015-02-25 | Novozymes A/S | Galactanase variants |
| DE60328715D1 (de) | 2002-12-20 | 2009-09-17 | Novozymes As | Polypeptide mit cellobiohydrolase ii-aktivität und dafür kodierende polynucleotide |
| DE602004030000D1 (de) | 2003-01-17 | 2010-12-23 | Danisco | Verfahren zur in-situ-herstellung eines emulgators in einem nahrungsmittel |
| ES2393058T3 (es) | 2003-05-02 | 2012-12-18 | Novozymes Inc. | Variantes de beta-glucosidasa |
| WO2005030926A2 (en) | 2003-08-25 | 2005-04-07 | Novozymes Inc. | Variants of glycoside hydrolases |
| DE602004030595D1 (de) | 2003-10-10 | 2011-01-27 | Novozymes As | Proteasevarianten |
| JP4891220B2 (ja) | 2004-02-25 | 2012-03-07 | ノボザイムス アクティーゼルスカブ | 菌類細胞壁分解酵素 |
| GB0405637D0 (en) | 2004-03-12 | 2004-04-21 | Danisco | Protein |
| US9045748B2 (en) | 2004-05-27 | 2015-06-02 | Novozymes, Inc. | Methods for transforming and expression screening of filamentous fungal cells with a DNA library |
| CN101052702B (zh) | 2004-07-16 | 2013-01-09 | 杜邦营养生物科学有限公司 | 脂肪分解酶及其在食品工业中的应用 |
| CN107667177A (zh) | 2015-04-07 | 2018-02-06 | 诺维信公司 | 用于选择具有增强活性的酶的方法 |
| WO2016164596A2 (en) | 2015-04-07 | 2016-10-13 | Novozymes A/S | Methods for selecting enzymes having lipase activity |
| CN109312333A (zh) | 2016-04-07 | 2019-02-05 | 诺维信公司 | 选择具有蛋白酶活性的酶的方法 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5605793A (en) * | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
-
1998
- 1998-03-18 AU AU66116/98A patent/AU738461B2/en not_active Ceased
- 1998-03-18 DK DK98907912.4T patent/DK1015575T3/da active
- 1998-03-18 NZ NZ337910A patent/NZ337910A/en unknown
- 1998-03-18 AT AT98907912T patent/ATE468394T1/de not_active IP Right Cessation
- 1998-03-18 IL IL13198698A patent/IL131986A0/xx unknown
- 1998-03-18 EP EP98907912A patent/EP1015575B1/en not_active Revoked
- 1998-03-18 JP JP54004498A patent/JP2001516216A/ja active Pending
- 1998-03-18 WO PCT/DK1998/000105 patent/WO1998041623A1/en not_active Ceased
- 1998-03-18 DE DE69841674T patent/DE69841674D1/de not_active Expired - Lifetime
- 1998-03-18 CA CA002284097A patent/CA2284097A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| DE69841674D1 (de) | 2010-07-01 |
| ATE468394T1 (de) | 2010-06-15 |
| JP2001516216A (ja) | 2001-09-25 |
| WO1998041623A1 (en) | 1998-09-24 |
| EP1015575A1 (en) | 2000-07-05 |
| EP1015575B1 (en) | 2010-05-19 |
| AU738461B2 (en) | 2001-09-20 |
| IL131986A0 (en) | 2001-03-19 |
| DK1015575T3 (da) | 2010-08-23 |
| NZ337910A (en) | 2001-10-26 |
| AU6611698A (en) | 1998-10-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Discontinued |