CA2277817A1 - Procedes et compositions a deux composants induisant la letalite cellulaire vegetale - Google Patents

Procedes et compositions a deux composants induisant la letalite cellulaire vegetale Download PDF

Info

Publication number
CA2277817A1
CA2277817A1 CA002277817A CA2277817A CA2277817A1 CA 2277817 A1 CA2277817 A1 CA 2277817A1 CA 002277817 A CA002277817 A CA 002277817A CA 2277817 A CA2277817 A CA 2277817A CA 2277817 A1 CA2277817 A1 CA 2277817A1
Authority
CA
Canada
Prior art keywords
plant
cell
polypeptide
expression cassette
promoter
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002277817A
Other languages
English (en)
Inventor
Neal Gutterson
Ed Ralston
Diane Burgess
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DNA Plant Technology Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2277817A1 publication Critical patent/CA2277817A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/8263Ablation; Apoptosis
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213Targeted insertion of genes into the plant genome by homologous recombination
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8217Gene switch
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237Externally regulated expression systems
    • C12N15/8239Externally regulated expression systems pathogen inducible
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289Male sterility
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases RNAses, DNAses

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Communicable Diseases (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Cultivation Of Plants (AREA)

Abstract

La présente invention concerne des procédés permettant d'inhiber la croissance d'une cellule ou de tuer une cellule dans un organisme, en particulier chez les plantes. L'invention se rapporte également à des cellules génétiquement modifiées permettant de tuer des cellules spécifiques ou d'exercer un effet bénéfique sur des cellules spécifiques.
CA002277817A 1997-01-24 1998-01-23 Procedes et compositions a deux composants induisant la letalite cellulaire vegetale Abandoned CA2277817A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US3648397P 1997-01-24 1997-01-24
US6598997P 1997-11-14 1997-11-14
US60/036,483 1997-11-14
US60/065,989 1997-11-14
PCT/US1998/001315 WO1998032325A1 (fr) 1997-01-24 1998-01-23 Procedes et compositions a deux composants induisant la letalite cellulaire vegetale

Publications (1)

Publication Number Publication Date
CA2277817A1 true CA2277817A1 (fr) 1998-07-30

Family

ID=26713211

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002277817A Abandoned CA2277817A1 (fr) 1997-01-24 1998-01-23 Procedes et compositions a deux composants induisant la letalite cellulaire vegetale

Country Status (5)

Country Link
EP (1) EP1006780A4 (fr)
JP (1) JP2002514065A (fr)
AU (1) AU744675B2 (fr)
CA (1) CA2277817A1 (fr)
WO (1) WO1998032325A1 (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9703681D0 (en) 1997-02-21 1997-04-09 Gene Shears Pty Ltd Protein complemention
US6852911B1 (en) * 1999-08-31 2005-02-08 Fertiseed Ltd. Method of producing a male sterile plant by exogenic allelism
GB0025225D0 (en) * 2000-10-14 2000-11-29 Cambridge Advanced Tech Plant cell death system
US10787673B2 (en) 2007-02-01 2020-09-29 Enza Zaden Beheer B.V. Disease resistant Brassica plants
US11685926B2 (en) 2007-02-01 2023-06-27 Enza Zaden Beheer B.V. Disease resistant onion plants
EP2455480B1 (fr) 2007-02-01 2017-10-18 SciENZA Biotechnologies 2 B.V. Plantes résistantes aux maladies
WO2008092505A1 (fr) 2007-02-01 2008-08-07 Enza Zaden Beheer B.V. Plantes résistantes à la maladie
US10501754B2 (en) 2007-02-01 2019-12-10 Enza Zaden Beheer B.V. Disease resistant potato plants
EP2622102B1 (fr) * 2010-09-30 2019-03-27 Rigel Pharmaceuticals, Inc. Système rapporteur in vivo
DE102012003848A1 (de) * 2012-02-29 2013-08-29 Kws Saat Ag Pathogenresistente transgene Pflanze
EP2781151A1 (fr) 2013-03-18 2014-09-24 Bayer CropScience AG Procédés de séparation de graines hybrides à partir d'un mélange d'agents de contrôle biologique de graines
CN105492600B (zh) 2013-07-22 2020-08-07 生物科学技术第5有限公司 向日葵中的霜霉菌抗性提供基因
CN106573963B (zh) 2014-06-18 2021-07-20 安莎种子公司 疫霉属抗性的属于茄科的植物
US20210071195A1 (en) 2017-08-29 2021-03-11 Scienza Biotechnologies 3 B.V. Soybean plants resistant to phytophthora sojae

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE8605621D0 (sv) * 1986-12-30 1986-12-30 Biokonsult Ab Heteromultimeric proteins and their manufacture
NL9000773A (nl) * 1990-04-02 1991-11-01 Rijkslandbouwhogeschool Werkwijze voor het beschermen van planten tegen pathogenen.
ATE173765T1 (de) * 1990-07-20 1998-12-15 Pioneer Hi Bred Int Binäres cryptocytotoxisches verfahren zur herstellung von hybrid-saatgut
WO1993025695A1 (fr) * 1992-06-12 1993-12-23 Plant Genetic Systems N.V. Entretien de plantes steriles males
GB9216151D0 (en) * 1992-07-29 1992-09-09 Ici Plc Containment of plant germplasm
GB9310177D0 (en) * 1993-05-18 1993-06-30 Cambridge Advanced Tech Cell specific necrosis
GB9401780D0 (en) * 1994-01-31 1994-03-23 Nickerson Biocem Ltd Modified plants
US6013859A (en) * 1994-07-14 2000-01-11 Pioneer Hi-Bred International, Inc. Molecular methods of hybrid seed production

Also Published As

Publication number Publication date
WO1998032325A1 (fr) 1998-07-30
EP1006780A1 (fr) 2000-06-14
EP1006780A4 (fr) 2005-03-09
AU744675B2 (en) 2002-02-28
AU6248298A (en) 1998-08-18
JP2002514065A (ja) 2002-05-14

Similar Documents

Publication Publication Date Title
US7115798B1 (en) Methods for regulated expression of triats in plants using multiple site-specific recombination systems
CN107072165B (zh) 小麦Ms1多核苷酸、多肽以及使用方法
JP5944320B2 (ja) 最適化エンドヌクレアーゼおよびその使用
AU2020202241B2 (en) Manipulation of dominant male sterility
AU744675B2 (en) Two component plant cell lethality methods and compositions
JP2017535296A (ja) ゲノム編集のための核酸構築物
AU2019240727B2 (en) Compositions and methods comprising male fertility sequences
WO2011064751A1 (fr) Endonucléases chimériques et utilisations de celles-ci
CZ274394A3 (en) Promotor elements of chimeric genes of alpha-tubulin, nucleic acids and chimeric genes containing such elements, and their use for transformation of plants
WO1993001283A1 (fr) Plantes transgeniques sans genes de selection
EP3270685A1 (fr) Procédés et compositions permettant d'accélérer l'introgression de caractères
US6392119B1 (en) Two component plant cell lethality methods and compositions
US7238854B2 (en) Method of controlling site-specific recombination
EP2800810A1 (fr) Recombinaison in planta
US20170240911A1 (en) Agrobacterium-mediated site specific integration
WO2018082611A1 (fr) Construction d'acide nucléique exprimant un gène exogène dans des cellules végétales et utilisation associée
WO2001007590A2 (fr) Nouveaux gènes chimères
EP1009843A1 (fr) Expression selective des genes chez les vegetaux
WO2020171192A1 (fr) Acide nucléique pour l'édition du génome d'une cellule végétale et son utilisation
López-Meyer et al. Transformation
US20010007154A1 (en) Materials and methods for hybrid seed production
CA2517856A1 (fr) Elimination de sequences de plastides a l'aide de recombinases specifiques de sites, a expression transitoire
CA2975709A1 (fr) Modification du genome mediee par agrobacterium sans integration de l'adn-t

Legal Events

Date Code Title Description
FZDE Discontinued