CA2272584C - Novel substituted pyrazole derivatives for the treatment of cardiocirculatory diseases - Google Patents

Abstract

The present invention relates to novel substituted pyrazole derivatives, a method for the production and the use thereof as a medicament, specially as a medicament to treat cardiocirculatory diseases.

Description

Le A 32 120-Foreign countries / R/Kr/SF' - .. - _ , . ...__~
:' --.E

New substituted pvrazole derivatives The present invention relates to new substituted pyrazole derivatives, processes for their preparation and their use as medicaments, in particular as medicaments for the treatment of cardiovascular disorders.

It has already been disclosed that 1-benzyl-3-(substituted heteroaryl)-fused pyrazole derivatives inhibit platelet aggregation (cf. EP 667 345 Al).

The present invention relates to new substituted pyrazole derivatives of the general formula (I) NI ~ (I) N FZa I
CHZ =A
in which R' represents a saturated or aromatic 5- or 6-membered heterocycle having up to 3 heteroatoms from the group consisting of S, N and/or 0, which can be bonded via a nitrogen atom and 'which is optionally substituted up to 3 times identically or differently by aimino, azido, formyl, mercaptyl, carboxyl, hydroxyl, straight-chain or branched acyl, alkoxy, alkylthio or alkoxycarbonyl each having up to 6 carbon atonis, nitro, cyano, halogen, phenyl or straight-chain or branched alkyl having up to 6 carbon atoms, which for its part can be substituted by hydroxyl, amino, az:ido, carboxyl, straight-chain or branched acyl, alkoxy, alkoxycarbonyl or acylamino each having up to 5 carbon atoms or by a radical of the formula -OR , Le A 32 120-Foreign countries in which Ra denotes straight-chain or branched acyl having up to 5 carbon atoms or a group of the formula -SiR5R6R7 , in which R5, R6 and R' are identical or different and denote aryl having 6 to carbon atoms or alkyl having up to 6 carbon atoms, and/or is substituted by a radical of the formula H2 ~ )(CH2)b CH3 I
I or OCH2 - (CHz)s C)(CH2)o -CH3 N' OR

-S(O),-NR9R10, 10 in which a, b and b' are identical or different and denote a number 0, 1, 2 or 3, ...

Rg denotes hydrogen or straight-chain or branched alkyl having up to 4 carbon atoms, c denotes a number ]. or 2 and R9 and R10 are identical or different and denote hydrogen or straight-chain or branched alkyl having up to 10 carbon atoms, which can optionally be substituted by cycloalkyl having 3 to 8 carbon atoms or by aryl having 6 to 10 carbon atoms, which for its part can be substituted by halogen, or Le A 32 120-Foreign countries denote aryl having 6 to 10 carbon atoms, which is optionally substituted by halogen, or denote cycloalkyl having 3 to 7 carbon atoms, or R9 and R10, together with the nitrogen atom, form a 5- to 7-membered saturated heterocycle which can optionally contain a further oxygen atom or a radical -NR", in which R" denotes hydrogen, straight-chain or branched alkyl having up to 4 carbon atoms or a radical of the formula / O
> or denotes benzyl or phenyl, ~ O

where the ring systems are optionally substituted by halogen, R2 and R', including the double bond, form a 6-membered saturated or aromatic heterocycle having up to 3 heteroatoms from the group consisting of N, S
and/or O, which is optionally substituted up to 3 times identically or differently by formyl, carboxyl, hydroxyl, mercaptyl, straight-chain or branched acyl, ,-.
alkylthio or alkoxycarbonyl each having up to 6 carbon atoms, nitro, cyano, halogen or straight-chain or branched alkyl or alkoxy each having up to 6 carbon atoms, which for its part can be substituted by hydroxyl, amino, carboxyl, straight-chain or branched acyl, alkoxy or alkoxvcarbonyl each having ?0 up to 5 carbon atoms, and/or the heterocycle is optionally substituted by a group of the formula -NR''R13 or -S(O)c=NR9'R10', in which R'' and R13 are identical or different and denote hydrogen or straight-chain or branched alkyl having up to 6 carbon atoms, or Le A 32 120-Foreign countries R''- denotes hydrogen and R13 denotes formyl c', R" and R10' have the meaning; of c, R9 and R'0 indicated above and are identical to or different from these and/or the heterocycle is optionally substituted by phenyl which for its part can be substituted up to 2 times identically or differently by halogen or by straight-~ chain or branched alkyl or alkoxy each having up to 6 carbon atoms and/or the heterocycle is optionally substituted by a group of the formula -N=CH-NR'aR'S, in which R14 and R1' are identical or different and denote hydrogen, phenyl or straight-chain or branched alkyl having up to 6 carbon atoms, A represents a 5- or 6-membered aromatic or saturated heterocycle having up to 3 heteroatoms from the group cotisisting of S, N and/or 0 or phenyl, each of which is optionally substituted up to 3 times identically or differently by amino, mercaptyl, hydroxyl, formyl, carboxyl, straight-chain or branched acyl, alkylthio, alkyloxyacyl, alkoxy or alkoxycarbonyl each having up to 6 carbon atoms, nitro, cvano, trifluoromethyl, azido, halogen, phenyl or straight-chain or branched alkyl having up to 6 carbon atoms, which for its part can be substituted by hydroxyl, carboxy:l, straight-chain or branched acyl, alkoxy or alkoxycarbonyl each having up tc- 5 carbon atoms, and/or is substituted by a group of the formula -(CO)d NR16R", in which d denotes a number 0 or 1, . ,.

Le A 32 120-Foreign countries R16 and R" are identical or different and denote hydrogen, phenyl, benzyl or straight-chain or branched alkyl or acyl each having up to 5 carbon atoms, and their isomeric forms and salts.

The compounds of the general formula (I) according to the invention can also be present in the form of their salts. In general, salts with organic or inorganic bases or acids may be mentioned here.
~-.

In the context of the present invention, physiologically acceptable salts are preferred.
Physiologically acceptable salts of the compounds according to the invention can be salts of the substances according to the iiivention with mineral acids, carboxylic acids or sulphonic acids. Particularly preferred salts are, for example, those with hydrochloric acid, hydrobromic acid, sulphuric acid, phosphoric acid, methanesulphonic acid, ethane-sulphonic acid, toluenesulphonic acid, benzenesulphonic acid, naphthalenedisulphonic acid, acetic acid, propionic acid, lactic acid, tartaric acid, citric acid, fumaric acid, maleic acid or benzoic acid.

Physiologically acceptable salts can also be metal or ammonium salts of the compounds according to the invention which have a free carboxyl group. Those particularly preferred are, for example, sodium, potassium, magnesium or calcium salts, and also ammonium salts which are derived from ammonia, or organic amines such as, for example, ethylamine, di- or triethylamine, di- or triethanolamine, dicyclohexylamine, dimethylaminoethanol, arginine, lysine or ethylenediamine.

The compounds according to the invention can exist in stereoisomeric forms which either behave as image and mirror image (enantiomers), or which do not behave as image and mirror image (diastereomers). The invention relates both to the enantiomers or diastereomers and their respective rnixtures. Like the diastereomers, the racemic forms can also be separated into the stereoisomerically uniform constituents in a known manner.

Le A 32 120-Foreign countries Heterocycle in the context of the invention, depending on the abovementioned substituents, in general represents a saturated or aromatic 5- or 6-membered heterocycle which can contain up to 3 heteroatoms from the group consisting of S. N and/or 0 and which in the case of a nitrogen atom can also be bonded via this. Examples which may be mentioned are: oxadiazolyl, thiadiazolyl, pyrazolyl, pyridyl, pyrimidinyl, pyridazinyl.
pyrazinyl, thienyl, furyl, pyrrolyl, tetrahydropyranyl, tetrahydrofuranyl, 1,2,3-triazolyl, thiazolyl, oxazolyl, imidazolyl, morpholinyl or piperidyl. Thiazolyl, furyl, oxazolyl, pyrazolyl, triazolyl, pyridyl, pyrimidinyl, pyridazinyl and tetrahydropyranyl are preferred.
,-.

Preferred compounds of the general formula (I) according to the invention are those in which R' represents pyrimidinyl, pyridazinyl, pyridyl, pyrazinyl, tetrahydropyranyl, tetra-hvdrofuranyl, pyrrolyl, furyl, thienyl, imidazolyl, oxazolyl, thiazolyl, 1.2.3-triazolyl, pyrazolyl, oxadiazolyl, thiadiazolyl, isoxazolyl, isothiazolyl, pyranyl or morpholinyl, each of which is optionally substituted up to 3 times identically or differently by amino, formyl, rnercaptyl, carboxyl, hydroxyl, straight-chain or branched acyl, alkoxy, alkylthio or alkoxycarbonyl each having up to 5 carbon atoms, nitro, cyano, azido, fluorine, chlorine, bromine, phenyl or straight-chain or branched alkyl having up to 5 carbon atoms, which for its part can be substituted by hydroxyl, amino, azido, carboxyl, straight-chain or branched acyl, alkoxy, alkoxycarbonyl or acylamino each having up to 4 carbon atoms or by a radical of the formula -OR4, in which R4 denotes straight-chain or branched acyl having up to 4 carbon atoms.
and/or by a radical of the formula Le A 32 120-Foreign countnes 0 ~ H2 J (CHZ)b -CH
I ~
-~ ~ I or OCH2 - (CH2)1 O(CH2)ti --CH3 N ' OR
-S(O),-NR9R10, in which a, b and b' are identical or different and denote a number 0, 1, 2 or 3, R8 denotes hydrogen or straight-chain or branched alkyl having up to 3 carbon atoms, c denotes a number 1 or 2 and R9 and R10 are identical or different and denote hydrogen or straight-chain or branched alkyl having up to 9 carbon atoms, which can optionally be substituted by cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cyclo-heptyl or naphthyl or phenyl, which for their part can be substituted by fluorine or chlorine, or denote phenyl or naphthyl, each of which is optionally substituted by fluorine or chlorine, or denote cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl or cycloheptyl, or R9 and R10, together with the n;itrogen atom, form a morpholine ring or a radical of the formula -N -N or -N N-R" , in which R" denotes hydrogen, methyl or a radical of the formula Le A 32 120-Foreign countries / C) >
~ or denotes benzyl or phenyl, where the ~ C) -c H2 ring systems are optionally substituted by fluorine or chlorine, R' and R3, including the double bond, form a pyridyl, pyrimidinyl, pyrazinyl or pyridazinyl ring, each of which is optionally substituted up to 3 times ~ identically or differently by forrnyl, carboxyl, hydroxyl, mercaptyl, straight-chain or branched acyl, alkylthio or alkoxycarbonyl each having up to 5 carbon atoms, nitro, cyano, azido, fluorine, chlorine, bromine or straight-chain or branched alkyl or alkoxy each having up to 5 carbon atoms, which for its part can be substituted by hydroxyl, amino, carboxyl, straight-chain or branched acyl, alkoxy or alkoxycarbonyl each having up to 4 carbon atoms, and/or the abovementioned heterocyclic rings are optionally substituted by a group of the formula -NR''-R13 or -S(O)c.NR"R10', in which R'' and R1' are identical or different and denote hydrogen or straight-chain or branched alkyl having up to 4 carbon atoms, or R'' denotes hydrogen and R'' denotes formyl c', R" and R10' have the meaning of c, R9 and R'0 indicated above and are identical to or different from these and/or the abovementioned heterocyclic rings are optionally substituted by phenyl, which for its part can be substituted by fluorine, chlorine, bromine or by straight-chain or branched alkyl or alkoxy each having up to 4 carbon atoms Le A 32 120-Foreign countries and/or the abovementioned heterocyclic rings are optionally substituted by a group of the formula _NN!R'4 RiS, in which R" and R15 denote hydrogen or straight-chain or branched alkyl having up to 4 carbon atoms, A represents thienyl, tetrahydropyranyl, tetrahydrofuranyl, phenyl, morpholinvl, pyrimidyl, pyrazinvl, pyridazinyl or pyridyl, each of which is optionally substituted up to 2 times identically or differently by hydroxyl, formyl, carboxyl, straight-chain or branched acyl, alkylthio, alkoxyacyl, alkoxv or alkoxycarbonyl each having up to 4 carbon atoms, fluorine, chlorine, bromine, nitro, cyano, trifluoromethyl or stiraight-chain or branched alkyl having up to 4 carbon atoms, which for its part can be substituted by hydroxyl, carboxyl, straight-chain or branched acyl, alkoxy or alkoxycarbonyl each having up to 4 carbon atoms, and/or by a group of the formula '-(CO)d-NR16R", in which d denotes a number 0 or 1, R16 and R" are identical or different and denote hydrogen, phenyl, benzyl or straight-chain or branched alkyl or acyl each having up to 4 carbon atoms, and their isomeric forms and salts.

Particularly preferred compounds of the general formula (1) according to the invention are those Le A 32 120-Foreil?n countries in which R' represents imidazolyl, furyl, pyridyl, pyrrolyl, pyrazinyl, pyrimidyl, isoxazolyl, oxazolyl or thiazolyl, each of which is optionally substituted up to 3 times identically or differently by formyl, fluorine, chlorine, amino, mercaptyl, cyano, straight-chain or branched acyl, alkylthio, alkoxy or alkoxvcarbonyl each havina up to 4 carbon atoms or straight-ct:Lain or branched alkyl having up to 4 carbon atoms, which for its part can be substituted by hydroxyl, carboxyl, amino, azido, straight-chain or branched acyl, alkoxy, alkoxycarbonyl or acylamino each having up to 3 carbon atomsõ

and/or by a radical of the formula \ I -CH2 ~ O(CH2)o__CH3 or OCH2 - (CH)a O(CHZ)o --CH3 N , ORe -S(O)c-NR9Rt0, in which ,...
a, b and b' are identical or different and denote a number 0, 1 or 2, R 8 denotes hydrogen or methyl, c denotes a number 1 or 2 a:nd R and R10 are identical or different and denote hydrogen or straight-chain or branched alkyl having up to 9 carbon atoms, which can optionally be substituted by phenyl or naphthyl, or denote phenyl or naphthyl, each of which is optionally substituted by fluorine or chlorine, or Le A 32 120-Foreign countries denote cyclopropyl or cycloheptyl, or R9 and R10, together with the nitrogen atom, form a morpholine ring or a radical of the formula -N -N or -N \ N-R" in which . ~~
R" denotes hydrogen, methyl or a radical of the formula ,=. / O
1 > or denotes benzyl or phenyl, where the ring ~ O
-CHZ
systems are optionally substituted by chlorine, R2 and R3, including the double bond, form a pyridyl, pyrazinyl, pyrimidinyl or pyridazinyl ring, each of which is optionally substituted up to 3 times identically or differently by formyl, mercaptyl, carboxyl, hydroYyl, straight-chain or branched acyl, alkoxy, alkylthio or alkoxycarbonyl each having up to 4 carbon atoms, nitro, cyano, fluorine, chlorine or straight-chain or branched alkyl or alkoxy each having up to 3 carbon atoms, which for its part can be substituted by hydroxyl, amino, carboxyl, straight-chain or branched acyl.
alkoxy or alkoxycarbonyl each having up to 3 carbon atoms, and/or the heterocyclic rings are optionally substituted by amino, N,N-dimethyl-amino or by a radical of the formula -NH-CHO or -N=CH-N(CH3)1 and/or by phenyl, which for its part can be substituted by a radical of the formula -O(CH,),-CHj, A represents tetrahydropyranyl, phenyl, pyrimidyl, thienyl or pyridyl, each of which is optionally substituted up to 2 times identically or differently by formyl, carboxyl, straight-chain or branched acyl, alkylthio, alkyloxyacyl.

Le A 32 120-Foreign countries alkoxy or alkoxycarbonyl each having up to 3 carbon atoms, fluorine, chlorine, bromine, nitro, cyano, trifluoromethyl or straight-chain or branched alkyl having up to 3 carbon atoms, which for its part can be substituted by hydroxyl, carboxyl, straight-chain or branched acyl, alkoxy or alkoxycarbonyl each having up to 3 carbon atoms, and their isomeric forms and salts.

Very particularly preferred compounds of the general formula (I) according to the ~ invention are those in which A represents phenyl, pyrimidyl or fluorine-substituted phenyl or pyrimidyl and their isomeric forms and salts.

Processes for the preparation of the compounds of the general formula (I) according to the invention have additionally been found, characterized in that, depending on the various meanings of the heterocycles mentioned under R2 and R' above, [A] compounds of the general formula (II) R'-D (II) in which R' has the meaning indicated above, and D represents radicals of the formula Le A 32 120-Foreign countries CN or CN ~CI Na "'O~CN
CN

in which R18 represents C,-C4-alkyl, are converted by reaction with co;mpounds of the general formula (III) A-CH,-NH-NH, (III) in which A has the meaning indicated above in inert solvents, if appropriate in the presence of a base, into the compounds of the general formula (IV) or (IVa) CHz A CH2 A
H2N NN and H=N N~N
T (IV) I(IVa) R NC R' in which A and R' have the meaning indicated above, and, in the case of the compounds of the general formula (IVa), then cyclized with carboxylic acids, nitriles, formamides or guanidinium salts, and, in the case of the compounds of the general formula (IV), then cyclized with 1,3-dicarbonyl derivatives, their salts, tautomers, enol ethers or enamines, in the presence of acids and, if appropriate, under microwaves, Le A 32 120-Foreign countries or [B] in the case where R'- and R' together form a pyrazine ring, compounds of the general formula (IV) are first converted by nitrosation into the compounds of the general formula (V) A
LN"I N
M.
HzN 11 N
in which 0 A and R' have the meaning indicated above, in a second step, by means of a reduction, the compounds of the general formula (VI) A
LN'N~
(VI), in which HZN NH2 ,-.

A and R' have the meaning indicated above, are prepared and finally cyclized with 1,2-dicarbonyl compounds, preferably aqueous glyoxal solution, or [C] compounds of the general formula (VII) Le A 32 120-Foreien countries I

N (VH) in which R

A', R'- and R3 have the meaning indicated above, and L represents a radical of the formula -SnR19R20R21, ZnR", iodine, bromine or triflate, in which R19, R'-0 and R'' are identical or different and denote straight-chain or branched alkyl having up to 4 carbon atoms, and R'-'- denotes halogen, are reacted with compounds of the general formula (VIII) R'-T (VIII) in which R' has the meaning indicated above and Le A 32 120-Foreign countries if L = SnR19R20R2' or ZnRZ'-, T represents triflate or halogen, preferably bromine, and if L = iodine, bromine or triflate, T represents a radical of the formula SnR19'R'0'R-' , ZnR22' or B-R''R-4 , in which R19', R'-0' , R''" and R"' have the meaning of R19, R'-0, R'-' and R'-' indicated above and are identical to or different from these, R'-3' and R'4' are identical or different and denote hvdroxyl, aryloxy having 6 to 10 carbon atoms or straight-chain or branched alkyl or alkoxy each having up to 5 carbon atoms, or together form a 5- or 6-membered carbocyclic ring, in a palladium-catalysed reaction in inert solvents, if appropriate in the presence of a base, [D] if R' _ _4 O

OH
in which Le A 32 120-Foreign countries R25 denotes (C,-C6)-alkyl which is optionally substituted by halogen, compounds of the general formula. (IX) C'C)-CI (IX), I N'N
in which A

A, R'- and R3 have the meaning indicated above, are converted either directly by reaction with the compound of the formula (X) Rss HZN { (~
in which ci I CI

R'-5 has the meaning indicated above, in the system NaOCO-CH3/N-methylpyrrolidine into the compounds of the general formula (Ia) RZ
R' Rzs / / ~~ I (Ia), /N,N O
IA oAc in which R'-, R' and A and R25 have the meaning indicated above, and then, by action of potassium hydroxide in methanol, the acetyl group is removed, Le A 32 120-Foreign countries or first by reaction of the compounds of the general formula (IX) with the compound of the formula (X) the compounds of the general formula (XI) R2s N, N CO--NH (XI), A
in which CI C!
.-, R'-. R3, A and R'S have the meaning indicated above, are prepared, and in a further step by action of potassium hydroxide the hydroxymethyl compounds are prepared, and in the case of the groups -S(O),NR9R10 and -S(O)c-NR"R", starting from the unsubstituted compounds of the general formula (I), first reacted with thionyl chloride and in a second step with the appropriate amines and, if appropriate, the substituents mentioned under R', R'-, R' and/or A are varied or introduced according to customary methods, preferably by chlorination, catalytic hydrogenation, reduction, oxidation, removal of protective groups and/or nucleophilic substitution.

The heterocycles mentioned under R' and R' can also be introduced by reaction of the appropriately substituted compounds of the general formula (II) according to other known heterocyclic syntheses.

The process according to the invention can be illustrated by way of example by the following reaction scheme:

Le A 32 120-Foreign countries [A]
~
o_CH, ~ i CN H2N N"N
ur\r i CN C6H ~" NH

O
~ \
N ~ POGs / N N
,., HCOOH ~ N N 0 Nr' N
HN

O O O
t/ \
~
N
-N
Pd(OH)2 / H2 N POC131 DMF
~
Vilsmeier formylation N(C2Hs)3, catat. hydrogenation ~ O

o / \
~
~N N N N
N I ~ N NaBH4 IN( / N

O / O
OH
CHO

Le A 32 120-Foreign countries -:20-[A]

I /
HO 0 H2N-NH-=CH2-C6Hs \
O
~ CN H2N \ N ~N

O
\'k OH
~ CF3COOH N
N
H \ H ~\ I ~N
' 0 O'Na' O
Malonaldehyde derivative or salts of the tautomers OH
[A] and derivatization p ~ \ / /
H~N N F Ph N ~N F DMF ~ ~ N
N~ F
PhCN
I N N /N POCh N~ I N
NC

O O O
N(CH3)?.
CHO
~
NH- NHZ HCO~ I /

~ j N F
'N
~ / NI~

H.N~N N'N F HN ~.-N

NH

Le A 32 120-Foreign countries (A]

\

~ _.
H2N N,i F COZCZHS HO j N, N F
O
O
[A]

\
( N NH?. I N F

N - N O Oc~~ CN-CH2-O
HZN-NH-CH2 --p F
CN~ N
~ p CN

Le A 32 120-Foreign countries [B]

1.) HNO2 HZN N, I F 2.) Pd-C/1~I2 N N F
3.) Glyoxa_I CN
N
LU O
OH ~ 0 OH
[C]

N
N

F
F I \ Pd(PPh3)2C1~ \
N iN -N
Sn-CH3 N
H3C~ ci L/ CFi, .~-Le A 32 120-Foreign countries [D]

N\ N\
O H2N ICH3 NaOAc N H, N, NM--~ N' N
F CI CI cl 1so'C N O
F
I I OAc KOH
- MeOH
N\ /

or via N, /
N
F NH--,,CHl \

/ ( I~ N
N CH
\ C1 CI RIN, N O
F
OH
\

Suitable solvents here for the individual steps of the processes are inert organic solvents which do not change under the reaction conditions.
~ These include ethers, such as diethyl ether or tetrahydrofuran, DME, dioxane, alcohols such as methanol and ethanol, halogenohydrocarbons such as dichloromethane, trichloromethane, tetrachloromethane, 1,2-dichloroethane, trichloroethane, tetrachloroethane, 1,2-dichloroethane or trichloroethylene, hvdrocarbons such as benzene, xylene, toluene, hexane, cyclohexane or petroleum fractions, nitromethane, dimethylformamide, acetone, acetonitrile or hexamethylphosphoramide. It is also possible to employ mixtures of the sollvents. Tetrahydrofuran, dimethylformamide, toluene, dioxane or dimethoxyethane are particularly preferred.

Bases employed for the processes according to the invention can in general be inorganic or organic bases. These preferably include alkali metal hydroxides such as, for example, sodium hydroxide or potassium hydroxide, alkaline earth metal hydroxides Le A 32 120-Foreign countries such as, for example, barium hydroxide, alkali metal carbonates such as sodium carbonate or potassium carbonate, alkaline earth metal carbonates such as calcium carbonate, or alkali metal or alkaline earth metal alkoxides such as sodium or potassium methoxide, sodium or potassium ethoxide or potassium tert-butoxide.
or organic amines (trialkyl(C,-C6)-amines) siach as triethylamine, or heterocycles such as 1.4-diazabicyclo[2.2.2] octane (DABCO), 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU), pyridine, diaminopyridine, methylpiperidine or morpholine. It is also possible to employ as bases alkali metals such as sodium and their hydrides such as sodium hydride. Sodium and potassium carbonate, triethylamine and sodium hydride are preferred.

The base is employed in an amount frorn 1 mol to 5 mol, preferably from I mol to 3 mol, relative to 1 mol of the compounci of the general formula (II).

The reaction is in general carried out in a temperature range from 0 C to 150 C, preferably from +20 C to +1 10 C.

The reaction can be carried out at normal, elevated or at reduced pressure (e.g. 0.5 to 5 bar). In general, it is carried out at normal pressure.

Suitable acids for the cyclization are in general protonic acids. These preferably include inorganic acids such as, for example, hydrochloric acid or sulphuric acid, or organic carboxylic acids having 1- 6 C atoms, optionally substituted by fluorine, chlorine and/or bromine, such as, for example, acetic acid, trifluoroacetic acid, trichloroacetic acid or propionic acid, or sulphonic acids having C,-C,,-alkyl radicals or aryl radicals such as, for example, methanesulphonic acid, ethanesulphonic acid, benzenesulphonic acid or toluenesulphonic acid.

The catalytic hydrogenation can in general be carried out by means of hydrogen in water or in inert organic solvents such as alcohols, ethers or halogenohydrocarbons, or mixtures thereof with catalysts such as Raney nickel, palladium, palladium on animal charcoal or platinum, or with hydrides or boranes in inert solvents, if appropriate in the Le A 32 120-Foreign countries - ?5 -presence of a catalyst.

Chlorination is in general carried out using the customary chlorinating agents such as, for example, PCI3, PC151 POC13 or elemental chlorine. POC13 is preferred in the context of the invention.

If the radicals of the formulae -S(O),NR9R10 and -S(O)r.NR 'R'0' are present, the corresponding unsubstituted compounds are first reacted with thionyl chloride.
In a further step, the reaction with the amines is carried out in one of the abovementioned ethers, preferably dioxane. If c = 2, an oxidation is then carried out according to customary methods. The reactions are carried out in a temperature range from 0 C to 70 C and normal pressure.

The nucleophilic substitutions and Vilsmeier reactions are carried out according to customary, published methods.

The reductions are in general carried out using reducing agents, preferably using those v.-hich are suitable for the reduction of carbonyl to hydroxyl compounds.
Reduction using metal hydrides or complex metal hyclrides in inert solvents is particularly suitable here, if appropriate in the presence of a trialkylborane. Preferably, the reduction is carried out using complex metal hydrides such as, for example, lithium borohydride, sodium borohydride, potassium borohydride, zinc borohydride, lithium trialkyl-borohydride, diisobutylaluminium hydride or lithium aluminium hydride.
Reduction with diisobutylaluminium hydride and sodium borohydride is very particularly preferred.

The reducing agent is in general employed in an amount from I mol to 6 mol, preferably from 1 mol to 4 mol relative to I mol of the compounds to be reduced.
The reduction in general proceeds in a teniperature range from -78 C to +50 C, prefer-ably from -78 C to 0 C in the case of DIF3AH, 0 C to room temperature in the case of NaBH4.

Le A 32 120-Foreign countries The reduction in general proceeds at normal pressure, but it is also possible to work at elevated or reduced pressure.

The compounds of the general formulae (II) and (III) are known per se or can be prepared by customary methods [cf for this: J. Hromatha et al., Monatsh. Chem.
1976.
~ 107, 233).

The compounds of the general formulae (IV), (IVa), (V) and (VI) are known in some cases and can be prepared as described above.
...

Suitable solvents here for process [C] are inert organic solvents which do not change under the reaction conditions. These include ethers, such as diethyl ether or tetrahydro-furan, DME, dioxane, halogenohydrocar=bons such as dichloromethane, trichloro-methane, tetrachloromethane, 1,2-dichloroethane, trichloroethane, tetrachloroethane, 1.2-dichloroethylene or trichloroethylene, hydrocarbons such as benzene, xylene, toluene, hexane, cyclohexane or petroleum fractions, nitromethane.
dimethylformamide, acetone. acetonitrile or hexamethylphosphoramide. It is also possible to employ mixtures of the solvents. Tetrahydrofuran, dimethylformamide, toluene, dioxane or dimethoxvethane is particularly preferred.

The reaction is in general carried out in, a temperature range from 0 C to 150 C, preferably from +20 C to +l 10 C.

The reaction can be carried out at normal, elevated or at reduced pressure (e.g. 0.5 to 5 bar). In general, it is carried out at nornnal pressure.

Suitable palladium compounds in the cor.itext of the present invention are in general PdCI,(P(C6H5)3),, palladium bis-dibenzylideneacetone (Pd(dba),), [l,l'-bis-(diphenyl-phosphino)ferrocene]-palladium(II) chloride (Pd(dppf)Cl,) or Pd(P(C6H;);)4.
Pd(P(C6Hs)3)4 is preferred.

The compounds of the general formula (VI11) are known per se or can be prepared by Le A 32 120-Foreign countries customary methods.

The compounds of the general formula (VII) are known in some cases or, in the case of the stannyls, are new and can then be prepared, for example, by reacting the compounds of the general formula (XII) I
R' N. I N (x1i) in which R L
R2, R3 and A have the meaning indicatecl above, L' represents triflate or halogen, preferably iodine, with compounds of the general formula i;XIII) (SnR19R20R21 ), (XIII) in which R'9, R'0, R'' have the meaning indicated above as described above with palladium catalysis.

The compounds of the general formulae (XII) and (XIII) are known per se or can be prepared by customary methods.

Process [D] according to the invention is carried out using one of the abovementioned bases, preferably in N-methylpyrrolidone, in a temperature range from 100 C to 200 C, preferably at 150 C.

Le A 32 120-Foreign countries The compounds of the general formulae (IX) and (X) are known or can be prepared by customary methods.

The compounds of the general formula (Ia) and (XI) are new and can be prepared as described above.

In the case in which typical protective groups are employed in the course of derivatiza-tion reactions, their removal is in general carried out in one of the abovementioned alcohols and/or THF or acetone, preferably methanol/THF in the presence of hydro-chloric acid or trifluoroacetic acid or toluenesulphonic acid in a temperature range from 0 C to 70 C, preferably at room temperature and normal pressure.

The compounds of the general formula (I) according to the invention show an unforeseeable, valuable spectrum of pharmacological action.

The compounds of the general formula (I) lead to a vasorelaxation/inhibition of platelet atigregation and to a blood pressure fall and also to an increase in the coronary blood flow. These actions are mediated via a direct stimulation of soluble guanylate cyclase and an intracellular cGMP increase. Additionally, the compounds according to the invention increase the action of substances which raise the cGMP level, such as, for example, EDRF (endothelium-derived relaxing factor), NO donors, protoporphyrin IX, arachidonic acid or phenylhydrazine derivatives.

They can therefore be employed in medicaments for the treatment of cardiovascular disorders such as, for example, for the treatment of high blood pressure and cardiac insufficiency, stable and unstable angina pectoris, peripheral and cardiac vascular disorders, arrhythmias, for the treatment of thromboembolic disorders and ischaemias such as myocardial infarct, stroke, transitory and ischaemic attacks, peripheral circulatory disorders, prevention of restenoses such as after thrombolysis therapy, percutaneous transluminal angioplasty (PTA), percutaneous transluminal coronary angioplasty (PTCA), bypass and also for the treatment of arteriosclerosis and disorders of the urogenital system such as, for example, prostate hypertrophy, erectile Le A 32 120-Foreign countries dysfunction and incontinence.

The invention moreover includes the cotnbination of the compounds of the general formula (I) according to the invention wilth organic nitrates and NO donors.

Organic nitrates and NO donors in the context of the invention are in general substances which display their therapeutic: action via the release of NO or NO
species.
Sodium nitroprusside, nitroglycerine, isosorbide dinitrate, isosorbide mononitrate, molsidomine and SIN-1 are preferred.

The invention additionally includes combination with compounds which inhibit the dearadation of cyclic guanosine monophosphate (cGMP). These are, in particular, inhibitors of phosphodiesterases 1, 2 and 5; nomenclature according to Beavo and Reifsnyder (1990) TiPS 11 p. 150 to 155. The action of the compounds according to the invention is potentiated and the desireci pharmacological effect is increased by these inhibitors.

To determine the cardiovascular actions, the following investigations were carried out:
in in vitro investigations on cells of vascular origin, the influx on guanylate cyclase-dependent cGMP formation was testeci with and without NO donor. The anti-aQgreaatory properties were shown on human platelets stimulated with collagen.
The vasorelaxant action was detetmined in rabbit aortal rings precontracted with phenyl-ephrine. The hypotentive action was investigated in anaesthetized rats.

Stimulation of soluble guanvlate cvclase in primarv endothelial cells Primary endothelial cells were isolated from rabbit aortas by treatment with collagenase soln. The cells were then cultured in culture medium at 37 C/5% CO2 until confluence was reached. For the investigations, the cells were passaged, inoculated into 24-hole cell culture plates and subcultured until reaching confluence (- 2 x 105 cells/hollow). For the stimulation of endothelial guanylate cyclase, the culture medium was aspirated and the cells were washed once with Ringer solution. After removing the Ringer solution, Le A 32 120-Foreiszn countries the cells were incubated for 10 minutes at 37 C/5% CO, in stimulation buffer with or without NO donor (sodium nitroprusside, SNP, 1 M). Following this, the test substances (final concentration I M) wer.e added to the cells by pipette and they were incubated for a further 10 minutes. After the end of the incubation time, the buffer solution was aspirated and cold stop buffer at 4 C was added to the cells. The cells were then lysed at -20 C for 16 hours. The supernatants containing the intracellular cGMP were then removed and the cGMP concentration was determined by means of the cGMP-SPA system (Amersham Buchler, Brunswick).

Table A

Ex. No. cGMP increase (%) 14 > 1000 17 > 1000 Vasorelaxant action in vitro Rabbits are anaesthetized by a blow to the neck and exsanguinated. The aorta is removed, freed from adhering tissue, divided into 1.5 mm wide rings and individually transferred under a pretension into 5 ml organ baths containing warm, carbogen aerated Krebs-Henseleit solution at 37 C of the following composition (mM): NaCI: 119;
KCI: 4.8; CaCl, x 2 H,O: 1; MgSO4 x 7 H7O: 1.4; KH1PO4: 1.2; NaHCO3: 25;
glucose: 10.
The contractility is detected using Statham UC2 cells, amplified and digitalized by means of A/D converters (DAS-1802 HC, Keithley Instruments Munich), and recorded Le A 32 120-Foreign countries in parallel on linear recorders. To produce a contraction, phenylephrine is added to the bath cumulatively in increasing concentration.

After several control cycles, the substance to be investigated is investigated in each further passage in increasing dosage in each case and the height of the contraction is compared with the height of the contraction achieved in the last preliminary passage.
From this, the concentration is calculated which is necessary in order to reduce the height of the control value by 50% (IC50). The standard administration volume is 5 t and the proportion of DMSO in the bath solution corresponds to 0.1%.
...

Table B

Ex. No. Aorta (IC50) M
14 1.8 13.0 16 1.7 ,.. Blood pressure measurements in anaesthetized rats 15 Male Wistar rats having a body weight of 300 - 350 g are anaesthetized with thiopental (100 mg/kg i.p.). After tracheotomy, a catheter is inserted in the femoral artery for blood pressure measurement. The substances to be tested are administered orally in various doses by means of stomach tube as a suspension in Tylose solution.

Le A 32 120-Foreign countries Table C

Ex. No. Dose Max. blood pressure fall Time (mg/kg) (mm Hg) (min) ,..

Inhibition of platelet aggregation in vitro For determination of the platelet aggregation-inhibiting action, blood from healthy subjects of both sexes was used. As an anticoagulant, 9 parts of blood were admixed to one part of 3.8% strength aqueous sodium citrate solution. By means of centrifugation, platelet-rich citrate plasma (PRP) was obtained from this blood (Jurgens/
Beller, Klinische Methoden der Blutgerinnungsanalyse [Clinical Methods of Blood Coagulation Analysis]; Thieme Verlag, Stuttgart, 1959).

For these investigations, 445 l of PRP and 5 l of the active compound solution were preincubated at 37 C in a water bath. The platelet aggregation was then determined at 37 C in an aggregometer by the turbiiiometric method (Born, G.V.R., J.
Physiol.
(London), 168, 178-195, 1963). For this purpose, the preincubated sample was treated with 50 l of coliagen, an aggregation-inducing agent, and the change in the optical density was determined. For quantitative evaluation, the maximum aggregation response was determined and from this the percentage inhibition compared to the control was calculated.

Le A 32 120-Foreign countries The compounds described in the present invention are also active compounds for the control of illnesses in the central nervous system which are characterized by disorders of the NO/cGMP system. In particular, they are suitable for the elimination of cognitive deficits, for the improvement of learning and memory power and for the treatment of Alzheimer's disease. They are also suitablle for the treatment of disorders of the central nervous svstem such as anxiety, tension and depressive states, central nervous system-related sexual dysfunctions and sleep disorders, and also for the regulation of pathological disorders of foodstuff, tea, coffee, alcohl, tobacco and addictive druc, intake.

Furthermore, these active compounds are also suitable for the regulation of the cerebral blood circulation and are thus effective agents for the control of migrane.

They are also suitable for the prophylaxis and control of the sequelae of cerebral infarcts (cerebral apoplexy) such as stroke, cerebral ischaemias and craniocerebral trauma. The compounds according to the invention can also be employed for the control of states of pain.

The present invention includes pharmaceutical preparations which, in addition to non-toxic, inert pharmaceutically acceptable excipients, contain one or more compounds according to the invention or which consist of one or more active compounds according to the invention, as well as processes foi- the production of these preparations.

The active compound(s) can optionally be present in one or more of the excipients indicated above and also in microencapsulated form.

The therapeutically active compounds should be present in the abovementioned pharmaceutical preparations in a concentration from approximately 0.1 to 99.5, preferably from approximately 0.5 to 95, % by weight of the total mixture.

In addition to the compounds accorcling to the invention, the abovementioned pharmaceutical preparations can also contain further pharmaceutical active compounds.

Le A 32 120-Foreign countries In general, it has proved advantageous both in human and in veterinary medicine to administer the active compound(s) in total amounts from approximately 0.5 to approximately 500, preferably 5 to 100, mg/kg of body weight every 24 hours, if appropriate in the form of several individual doses, to achieve the desired results. An individual dose contains the active compound(s) preferably in amounts from approxi-mately I to approximately 80, in particular 3 to 30, mg/kg of body weight.
Abbreviations MeOH = methanol E = ethanol EA = ethyl acetate T = toluene Ph = phenyl The numbers after the solvent abbreviations in the following tables under the column Rt. denote parts by weight.

.-~

-34a-The invention also provides uses of the compounds and compositions of the invention for (i) preparing a medicament for the treatment of cardiovascular or thrombolytic disorders, or ischaemias, and (ii) for the treatment of cardiovascular or thrombolytic disorders, or ischaemias.

The invention also provides commercial packages comprising a compound or composition of the invention and associated therewith instructions for the use thereof in the treatment of cardiovascular or thrombolytic disorders, or ischaemias.

Le A 32 120-Foreign countries - :35 -Starting compounds Example I A
5-Amino-l-benzyl-3-(5-hydroxymethyl-2-furyl)-pyrazole I ~

H
I N
.-~
~ ~IV
N
H

O
H44.1 g (817 mmol) of sodium methoxide is added with stirring to a solution of 79.5 g (408 mmol) of benzylhydrazine dihydroc:hloride in 1.3 1 of ethanol. After 15 min, 67.4 g (408 mmol) of 2-cyanomethylcarbonyl-5-hydroxymethylfuran are added and the mixture is stirred under reflux for 3 hours. After cooling, 1 1 of water is added, the ethanol part is evaporated in vacuo and t:he precipitated crystals are filtered off with suction. After washing with water and then with ether, the precipitate is dried over P,O;. 91 g (83% of theory) of product having an m.p. of 163 C are obtained.

The compounds shown in Table lA were prepared analogously:

Le A 32 120-Foreign countries Table 1 A:

F
N
PJ R
\
J-~
NFi2 Ex. No. R' m.p. [ C] Rf Yield [% of theory]
2A 2-furyl 124 0.63 (T 1 E 1) 49 3A 2-pyrimidinyl 178 0.49 (MeOH1E4) 66.5 4A 2-pyridyl 130 0.08 (T 1 E 1) 30 Example 5A

5-Amino-1-(2-fluorobenzyl)-3-(5-hydroxyrnethyl-2-furyl)-4-nitroso-pyrazole F pN O
C / OrH
H-N N
H O

g (34.8 mmol) of 5-amino-i-(2-fluorobenzyl)-3-(5-hydroxymethyl-2-furyl)-pyrazole are initially introduced in a mixture of 66 ml of ethanol and 26.7 ml of 5%
strength 10 aqueous hydrochloric acid, treated in the course of 5 minutes with 26.4 ml of a 15%
strength ethanolic solution of ethyl nitrite and stirred at room temperature for I h. The deep violet reaction solution is added to aqueous potassium carbonate solution and extracted with ethyl acetate. After evaporating the organic phase in vacuo, 8 g of the residue are obtained, which can be immediately reacted further. (Rf = 0.17, T
1 E 1, SiO,).

Le A 32 120-Foreign countries Example 6A

4.5-Diamino-l-(2-fluorobenzyl)-3-(5-hydroxymethyl-2-furyl)-pyrazole ' F , O
N - O, H
H-N N-H
H H

The compound from Example 5A (8 g) is dissolved in ethanol. treated with 0.5 g of 5% strength palladium on carbon and hydrogenated for 15 minutes in a Parr apparatus ~ at a hydrogen pressure of 2 bar. The solution is filtered off with suction through kieselguhr and used for the next batch (R.f = 0.21, T1E1, SiO,).

Example 7A
3-Amino-2-(2-fluorobenzyl)-pyrazole / I F N
I
N

/N--H
H

Was obtained analogously to the process described in the patent Fr. 1403372 (Chem.
Abstr. 1965, 63, 14871a).

Le A 32 120-Foreign countries Example 8A

1-(2-Fluorobenzyl)-pyrazolo[3,4-b]pyrimidine .~
F
1~1 N~N
\
N
~ 'I
32 g of 3-amino-2-(2-fluorobenzyl)-pyrazole are dissolved in 1.5 1 of dioxane and treated with 31.45 g of dimethylaminoaci-olein. The mixture is warmed to 50 C
and ~ 16.65 g of trifluoroacetic acid are then aclded. The mixture is boiled for 60 hours, the solvent is then evaporated in vacuo, the residue is treated with water and the mixture is extracted with ethyl acetate. The organic phase is dried using Na,S04 and evaporated in vacuo, and the residue is chromatographed on silica gel. After elution with toluene --+ toluene/ethyl acetate 9:1, 17.3 g(46.3 .'o of theory) of the title compound having an Rf of 0.69 (Si02, T,E,) are obtained.

Example 9A

3 -Bromo-l-(2-fluorobenzyl)-pyrazolo[3,4-b]pyrimidine QNF
NBr Le A 32 120-Foreijzn countries 8 g (35.2 mmol) of 1-(2-fluorobenzyl)pyrazolo[3,4-b]pyrimidine are dissolved in 284 ml of chloroform and treated slowly at room temperature with 14 g (87.3 mmol) of bromine. The mixture is stirred overnight and a further 1.2 ml of bromine are then added dropwise. After 2 h, the reaction iis terminated and evaporated in vacuo. The residue is treated with 20 ml of ethyl acetate and induced to crystallize.
After washing the crystals with ether, 7.5 g (70% of theory) of the title compound having an R,- of 0.2 (SiO,, toluene) are obtained.

Example l0A
1-(2-Fluorobenzyl)-3-trimethylstannylpyra.zolo[3,4-b]pyrimidine N
N
N F
~ n1C:H3 1.22 Q(4 mmol) of 3-bromo-l-(2-fluorobenzyl)pyrazolo[3,4-b]pyrimidine are dissolved in 200 ml of dioxane under argon and treated with 4.5 g(13.74 mmol) of hexamethyl-distannane and 1.2 g of tetrakis(triphenylphosphine)palladium. The mixture is stirred overnight at 100 C, added to water and extracted with ethyl acetate. The organic phase is dried using Na,SO4, evaporated in vacuo and chromatographed on silica gel.
After elution with toluene, 1.4 g (89.7% of theory) of the title compound having an R, of 0.074 (SiO,, toluene) are obtained.

Le A 32 120-Foreign countries Example 11A

NRY\

N

CI
CI

500 mg of 1-(2-fluorobenzyl)pyrazolo[3,4-b]pyridine-3-carboxylic acid (1.84 mmol) are partly dissolved in 10 ml of methylene chloride. 400 mg of N-(3-dimethylamino-propyl)-N'-ethylcarbodiimide hydrochloridle (2.3 mmol) are added. The mixture is stirred at RT for 10 min (complete solution), then a solution of 260 mg of 1,1-dichloro-3-amino-but-l-ene (1.84 mmol) in 5 ml of inethylene chloride is added dropwise.
After approximately 3 h, the mixture is concentrated and purified on silica gel (solvent:
cvclohexane/EA 1:1).340 mg(47%)of 1-(2-fluorobenzyl)-3-(1,1-dichlorobut-l-en-3-yl-amido)pyrazolo[3,4]pyridine are obtained, Rf 0.35 (cyclohexane:EA 2:1). MS
(ESI-POSITIVE): 417 (27, [M+Na]'); 415 (42., [M+Na]'); 395 (60, [M+H]'); 393 (100, [M+H] ).

..~

Le A 32 120-Foreign countries Preparation examples Example 1 1-(2-Fluorobenzyl)-3-(2-hydroxymethyl-2-furyl)pyrazolo[3,4-b]pyrazine F q-J~ O
~1 O
NN

The crude batch (8 g in 200 ml of ethanol) from Example 6A is treated with 4.61 g of a 40% strength aqueous glyoxal solution and stirred at room temperature for 10 h. The mixture is evaporated in vacuo and chromatographed on SiO, using toluene/ethyl acetate mixtures. After crystallization with ether, 0.57 g (7.6% of theory) of the title compound having an m.p. of 194 C is obtained.

Example 2 1-(2-Fluorobenzyl)-3-(2-fury l)-6-hydroxypyrazolo[3,4-b]pyridine F ,N O
N

N, HO~
7 g (27.2 mmol) of 5 -amino-l-(2-fluorobetlzyl)-3-(2-fury l)pyrazole, 3.94 g (27.3 mmol) of ethyl 3-ethoxyacrylate and 1.96 ml (27.3 mmol) of trifluoroacetic acid are intimately mixed and made to react for 2 minutes in a microwave oven. The mixture is added to Le A 32 120-Foreign countries a solution of 10 g of K,HPO4 in 500 ml of water and extracted with 500 ml of ethyl acetate. After drying of the organic phase with MgSO4, 30 g of silica gel are added and the mixture is evaporated in vacuo. The residue is chromatographed on a silica gel column using a toluene-ethyl acetate gradient. The first fraction is crystallized using ether and affords 1.8 g(21.4% of theory) of the title compound having an m.p.
of 250 C.

The compounds listed in Table I were prepared in analogy to the procedure of Example 2:
~

Table I

F rJ~N R~

OH

,., 10 Ex. No. R' R19 Yield R,lm.p. C
(% of theory) 3 * 2-furyl -CH; 24 226 4 2-pyridyl H 0.8 (EA) * from ethyl acetoacetate Le A 32 120-Foreign countrics Example 5 4-Amino-l-(2-fluorobenzyl)-3-(2-furyl)pyrazolo[3,4-d]pyrimidine F ,N 0 N

2 g (7.1 mmol) of 5-amino-4-cyano-l-(2-fluorobenzyl)-3-(2-furyl)pyrazole and 30 ml of formamide are stirred first at 100 C and then at 195 C for 3 h. The material crystallizing on cooling is filtered off with suction and washed with cold formamide, the residue is taken up in ethyl acetate ar-d the solution is washed with water. After drying and evaporating the organic phase, 2.13 g (97% of theory) of the title compound having an m.p. of 190 C are obtained. Rf = 0.07 (T 1 E 1).

The compounds listed in Table 2 are obtained in an analogous manner, for example.
from 2-propyloxyphenylnitrile, benzonitrile or guanidinium hydrogencarbonate:
Table 2 N
N'' NHZ
R2e>-N

Le A 32 120-Foreign countries Ex. R'-' R28 M.P. Rf Yield No. [ C] [% of theory]
6 F 2-propyloxyphenyl 187 0.6 (T 1 E 1) 42.7 7 F Ph 205 0.69 (T 1 E 1) 100 8 F NH, 205 0.22 (EA) 71 9 H H 174 0.15 (T1E1) 90 Example 10 1-Benzyl-3-(2-fury l)-4-hydroxypyrazolo [3,4-d] pyrimidine / o N
N
II a N
N OH O
J

6.14 g (23.2 mmol) of 5-amino-l-benzyl-4-cyano-3-(2-fury l)pyrazole (RF = 0.6) are boiled for 3.75 h in 100 ml of formic acid. The batch is then evaporated in vacuo. The residue is treated with water and the mixture is extracted by shaking with ethyl acetate.
The insoluble portion is filtered off with suction and affords 5.1 g of the target compound (m.p. = 242 C, Rf = 0.3, SiO,, 'toluene/ethyl acetate = 1:1). By evaporating the organic phase, further quantities can be isolated.

Le A 32 120-Foreign countries Example 11 1-Benzyl-4-chloro-3-(2-fury l)pyrazolo[3,4-d]pyrimidine N
N
N
II
N /

CI / O
i 6.6 g of 1-benzyl-3-(2-furyl)-4-hydroxypyrazolo[3,4-d]pyrimidine are boiled for 12 h in 100 ml of POC13. The mixture is evaporated in vacuo, the residue is stirred with aqueous K,HPO4 solution and the mixture is extracted with ethyl acetate. After drying using Na,SOa and concentrating in a rotary evaporator, 7.47 g of a solid are obtained xvhich can be reacted directlv for the next stage (Rf = 0.8, SiO,, toluene/ethyl acetate = 1:1).

Example 12 .-1-Benzyl-3-(2-furyl)pyrazolo[3,4-d]pyrimidine o r N N \
N
N

O
5.81 g of 1-benzyl-4-chloro-3-(2-fury l)pvrazolo[3,4-d]pyrimidine are dissolved in 450 ml of dioxane and hydrogenated in the Parr apparatus at a hydrogen pressure of 3 bar for 5 h using 4 g of 20% Pd(OH)2 on carbon after addition of 2.61 ml of triethyl-Le A 32 120-Foreign countries amine. After filtration through kieselguhr, evaporation and chromatography, 2.26 g of yellowish crystals are obtained (m.p. = 106 C, Rf = 0.2 toluene/ethyl acetate = 4:1).
Example 13 1-Benzyl-3-(5-hydroxymethyl-2-furyl)-1-H-pyrazolo[3,4-b]pyridine i ~
~

O
H~ 2.69 g(10 mmol) of 5-amino-l-benzyl-3-(5-hydroxymethyl-2-furyl)pyrazole and 1.4 a of malonaldehyde hydrate sodium salt are stirred at 100 C for 30 min in 100 ml of dioxane and slowly treated with 1.9 ml of trifluoroacetic acid during the course of 5.5 hours. The mixture is evaporated in vacuo, the residue is taken up in ethyl acetate.
the mixture is extracted by shaking with K,HPO, solution, and the organic phase is dried using Na,S04 and concentrated in vacuo in a rotary evaporator. The residue is chromatographed on silica gel. 200 mg (6.6% of theory) of crystals havinQ a melting point of 104 C are obtained.

The compounds mentioned in Table 3 are prepared in analogy to the abovementioned procedures:

Le A 32 120-Foreign countries Table 3 RZ'' u R N ( N\
/N
R3S ~

Ex. R29 R' R3o R31 R32 M.P. Rf Yield No. ~oCI (% of theory) 14 F o HTC*4 H H H 115 0.6 (EA) 1.1 15 F 0 H H H 45 0.69 68 (T1E1) 16 F N H H H 163 0.33 11 (T1E1) ,... N -17 F N H H H 71 0.65 41 (T1E1) 18 H 0 Me Me Mc 115 0.84 71 (T1E1) Le A 32 120-Foreign countries Table 3 - continuation Ex. R29 R' R30 R31 R32 m P Rf Yield No. (oC] (% of theory) 19 H p Me H Me 78 0.77 71 (T1E1) ,...

Example 20 3-(4,5-Dimethylpyrimidin-2-yl)-1-(2-fluorobenzyl)pyrazolo[3,4-b]pyrimidine N
N F
N

NN

1.4 g (3.59 mmol) of 1-(2-fluorobenzyl)-3-trimethylstannylpyrazolo[3,4-b]pyrimidine are boiled overnight in toluene under argon with 0.51 g(3.58 mmol) of 2-chloro-4.5-dimethylpyrimidine and 0.2 g (0.28 mmol) of bis(triphenylphosphine)dichloropalladium.
3 g of silica gel are added and the solvent is evaporated in vacuo. The residue is then chromatographed on silica gel and eluted with a toluene/ethyl acetate mixture.
0.34 a (28.4% of theory) of the title compound having an m.p. of 167 C and an Rf of 0.08 (SiO,, T4E) is obtained.

Le A 32 120-Foreign countries The example shown in Table 4 was prepared in an analogous manner.
N
N
N F
IR~

Table 4 ,~-Ex. No. R' N-Example 22 1-Benzyl-3-(5-formyl-2-furyl)pyrazolo[3,4-d]pyrimidine ~
/\ ~

(NN N

, o H
0.74 ml of POC13 is added at 0 C to 0.64 ml of DMF. The solidified mixture is allowed to come to room temperature andl 14 ml of 1,2-dichloroethane are added. A

Le A 32 120-Foreign countries solution of 2 g of 1-benzyl-3-(2-furyl)pyrazolo[3,4-d]pyrimidine (Rf = 0.45, SiO,, toluene/ethyl acetate = 1:1) in 14 ml of l, 2-dichloroethane is added dropwise at 15 C
to this solution and it is then warmed to 80 C. After 4 h, the entire batch is added dropwise to further Vilsmeier reagent, which was prepared from 1.5 ml of POC1;
and 1.3 ml of DMF, and stirred at 80 C for 24 h. The mixture is then added to a 50%
aqueous solution of K,HPO4 and briefly heated to 75 C with stirring. After extraction with ethyl acetate, drying of the organic phase, concentration in a rotary evaporator and chromatography on SiO,, 0.6 g (27% of theory) of an oil is obtained (Rf = 0.3, SiO,.
toluene/ethyl acetate = 1:1).
...

The examples from Table 5 are prepared in analogy to the abovementioned procedures.
Table 5 Yield Rf Ex. Structure M.P. (% of No. ( C) theory) 23 203 56.1 0.17 (EA) H O N-N

O
N N
H3C\N I~

I/

Le A 32 120-Foreiizn countries Table 5 - continuation M.P. Yield Rf (% of ( C) theory) F24[ tructure F 220 80,6 0.23 (EA) N_N t O~ N

H~C, N

25 F 205 100 0.61 (EA) N-N

~I
H3 C.N N/

26 F 225 22.1 0.74 (T/E = 2:1) N-'N ~ /
O /
O~ NI
H'N N 'J'N%\NiCl-la t I

Le A 32 120-Foreign countries -'_i2-Table 5 - continuation Ex. Structure M.P. Yield o Rf (% of No. ( C) theory) 27 F 220 75 0.34 (TtEI) ' Z N
N N
H'C\N /l/

,... H~C

28 157 79 0.44 ~ 0 -,/"CH3 (T1E1) I ~.
N I N ~
/N
CHN

H CNN O
a 29 118 13.09 0.49 9 (T4:E1) N O
N~ :C
N~ CH3 Le A 32 120-Foreipn countries -5:3-Table 5 - continuation Ex. Structure m P Yield Rf ( /o of No. ( C) theory) 30 125 27.56 0.46 (T4:E1) ~N O
O
N \ ~ \

31 123 93 0.63 ~ ~ (T1:E1) N O
F N O
N~

97 0.49 32 p O (T1:E1) O
F N
N\
I\\ h HO

Le A 32 120-Foreijzn countries Example 33 1-Benzyl-3-(5-hydroxymethyl-2-furyl)pyrazolo[3,4-d]pyrimidine r\) ~ N N\ I' N
N

'O

OH
0.6 g (1.97 mmol) of 1-benzyl-3-(5-formyl-2-furyl)pyrazolo[3,4-d]pyrimidine (Rf =
0.65, SiO,, ethyl acetate) is treated with 60 mg of NaBH4 with good stirring in 20 ml of 1-propanol at room temperature. After 15 min, 50 ml of water and 2.5 ml of glacial acetic acid are added. After partially conceritrating in a rotary evaporator, the mixture is extracted with ethyl acetate, and the extract is dried and concentrated in a rotary evaporator after addition of toluene. After chromatography on SiO,, 74.8 mg (12.4% of tlieorv) of product are obtained (m.p. 165 (:, R; = 0.43, SiO1, ethyl acetate).

~-- 10 The compounds shown in Table 6 are prepared in analogy to the procedure of Example 3 3:

Le A 32 120-Foreign countries - 5'i -Table 6 Ex. Structure M.P. Yield Rf No. ( /, of [ C] theory) 34 165 43.5 0.37 (EA) Z
N
fl'N
N

,.... O

OH
35 213 60 0.15 (EA) H'O N-N
O

...

36 F 207 63 0.21 (EA) H,0 N-N , O

Le A 32 120-Foreign countries Table 6 - continuation Yield Ex. Structure M.P. (% of Rf No. ( CI theory) 37 F 94 30 0,45 (EA) NC N-N
O /
\ / J
=~ HC~N N

38 F 205 27.6 0.11 (EA) HC N-N
o /
N

H N
H N H

39 F 225 23.5 0.29 (T1E1) O /
I
H'N \N
H

Le A 32 120-Foreign countries Table 6 - continuation Ex. Structure M.P. Yield (% of Rf No. [ C] theory) 40 F 189 23 0.43 (T1E1) C~~, ~ ~
~
N' N N
~--O\/NH O
~"
H

I
H

F O O- ti CH3 N,N, H
.
NII, N
0 , N H
/

Le A 32 120-Foreign countries Table 6 - continuation Ex. Structure Yield Rf (% of f No. ( C] theory) 6 15.46 0.25 (T i E l) 42 q 10 F ~ \ O
N O,H
=-= N~

O
O
.
CH:3 43 ~ 155 81.73 0.48 (T1E1) ,N\ O
N \ / O
N~ ~ C

H~C

Le A 32 120-Foreign countries Table 6 - continuation Ex. Structure m p Yield Rf (% of No. [ C] theory) 44 180 72.08 0.53 (T1E1) ~N O
N O
.=~ N CH3 H

45 0.21 (T1E1) HO N N
I \N F
~ O

H

Le A 32 120-Foreil?n countries Example 46 Preparation of 1-(2-fluorobenzyl)-3-[5-(piperidine-l-sulphinyl)furan-2-yl]-1 H-pyrazolo-[3,4-b]pyridine CN) \
S=0 F N'IN

,=-, -N~~
a) Preparation of 1-(2-fluorobenzyl)-3-[5-chlorosulphinylfuran-2-vl]pyrazolo[3,4-b]-pyridine CI
S=0 F N

0.85 g (2.86 mmol) of 1-(2-fluorobenzyl)-3-(2-furvl)pyrazolo[3,4-b]pyridine is stirred with 20 ml of thionyl chloride for 25 min at 70 C. The batch is then evaporated in vacuo and reacted further in crude form.

b) The above batch is taken up in 30 ml of dioxane, treated with 0.6 ml (about 6 mmol) of piperidine, vigorously shaken and allowed to stand overnight. It is added to water and the mixture is extracted by shaking with ethyl acetate.
After drying the organic phase using Na.,SO4, it is evaporated and the residue is chromatographed on silica gel using a toluene/ethyl acetate gradient. 0.49 g _ (40% of theory) of a brown, clear viscous svrup is obtained (Rf (SiO,; TIEI) 0.36).

Le A 32 120-Foreign countries - 61. -The compounds shown in Table 7 are prepared in analogy to the abovementioned procedures and that of Example 46.

Example 67 is prepared in analogy to the procedure of Example 20.
,..

Le A 32 120-Foreijzn countries Table 7 Ex. Structure Yield M.P. (% of Rt No. ( C) theory) 47 0 29.34 0.37 EA

F N'N\ \ \ H
N~ /

~ H-O

48 KIIII,N O 8.23 0.12 (T1E1) F
,H

\ \

N~ /
,-.

49 F 0 5 oil 10 0.37 (T1E1) 0 \NiH

NN~
N\

Le A 32 120-Foreign countries Table 7 - continuation Yield Ex. Structure M.P. (% of ~
No. ( C) theory) 50 a S 107 37 0.28 (T1E1) F

0 N" CFi3 N~N\ ~ 1 N~ /
.-~

51 0~' 126 27 0.43 (T1E1) Ct F0 N~N~ \ H
N~ /

52 KI-N o\ /- ~ 279 77 0.16 F S-N\- JN-L=H3 (EA/EtOH =
- O
\ \ 1 1) N53 /\ 0 69 56 0.49 (TIEI) \\ H
F S-N

N
N
N~ / H' Le A 32 120-ForeiQn countries Table 7 - continuation Ex. Structure m P Yield R-f ( /a of No. ( C) theory) 54 122 34 0.31 (T1:El) - o ~
N
N --N~

55 cH, oil 4,2 0.37 (T1E1) HN

F 50 11.0 N

Le A 32 120-Foreign countries Table 7 - continuation Ex. Structure m P Yield ~.
( / of No. ( C) theory) 56 H,C 136 76 0.33 (T1El) ~CH,1 HN~
S
~
F O ~O
_N
N NZ
Nx /

57 138 50 0.31 (TIEI) HN
~
S~O

N~
N~ /

Le A 32 120-Foreign countries Table 7 - continuation Ex. Structure M.P. Yield R, of No. (% ( C) theory) 58 F~C *-f CH3 109 59 0.38 (TlE1) HN
~
F O S~O
N' ~

N~
59 H3C 114 53 0.45 (TIEI) HN

F O SO

~-. N.' N--N~ /

Le A 32 120-Foreiizn countries Table 7 - continuation Ex. Structure M.P. Yield Rf (% of No. ( C) theory) 60 161 40 0.46 (T1E1) C) ~ HN

S
F I ~ e_N

N61 H3C 109 58 0.58 (T1E1) HN' ~, C'H~
:-O
S
~ O
F 0 ~

LN~N~ ~ ' N\ ~

Le A 32 120-Foreijzn countries Table 7 - continuation Yield g{
Ex. Structure m.P= ("/o of No. ( C) theory) 62 / c> oil 55 0.17 (TIEI) N

N
S~
F O ~o N~ \ \ \

N~

63 158 66 0.47 (T1E1) H N Z
S O
~. F e N

Le A 32 120-Foreign countries Table 7 - continuation Yield Rl_ Ex. Structure M.P. (% of No. ( C) theory) 64 H3C _ 132 60 0.41 (T1E1) S~~
F p O
I?. HN
~N~ ~\

N~ /

65 107 71 0.4 (T1E1) N
\ I
I / S

N~N~
N~

Le A 32 120-Foreign countries Table 7 - continuation ~.
Ex. Structure M.P. Yield (% of N ' (OC) theory) 66 Ci 110 62 0.57 (T1E1) \ HN

F I / 0 S~O

~= N67 P,"/ 106 6 0.23 N(hexane:EA
N CH3 3:1) ~
N A1,03 F ~
/ I
\

Le A 32 120-Foreign countries Example 68 1-(2-Fluorobenzyl)-3-(4-methyl-3-hydroxymethyloxazol-2-yl)pyrazolo[3,4b]pyridine / \ CH3 N / ' OH
~N O
F

330 mg of 1-(2-fluorobenzyl)-3-(1,1-dichlorobut-l-en-3-yl-amido)pyrazolo[3,4b]pyridine ~
(0.84 mmol), 1.7 ml of NaOH 1N (1.68 mmol) and 3.3 ml of 1-methyl-2-pyrrolidone are stirred overnight at 50 C, then allowed to cool. The mixture is treated with water and ethyl acetate. The organic phase is sepai-ated, dried over Na,SO4 and concentrated.
The mixture is dried in a high vacuum. The solid is then treated with cyclohexane/EA
2:1, crystals being formed. The crystals are filtered off with suction and stirred at RT
with ether. An insoluble impurity is separated. The ethereal solution is concentrated and chromatographically purified. 52.1 mg (18%) of 1-(2-fluorobenzyl)-3-(4-methyl-hydroxymethyloxazol-2-yl)pyrazolo[3,4b]pyridine are obtained. M.p. 145 C. Rf:
0.074 (cyclohexane:EA 22:1). NIS (ESI-POSITIVE): 339 (100, [M + H]').

Example 69 1-(2-Fluorobenzyl)-3 -(4-ethyl-3 -hydroxymethyloxazol-2-yl)pyrazolo [3,4b]pyridine N\ ~

N~ / ~
~
NI CHZ
N
F / I OH
\

The compound is prepared in analogy to the procedure of Example 68. Yield (52%
of theory), Rf 0.33 (hexane:EA 1:1).

Claims (14)

1. claims Substituted pyrazole derivatives of the general formula (I) in which R1 represents a saturated or aromatic 5- or 6-membered heterocycle having up to 3 heteroatoms from the group consisting of S, N and/or O, which can be bonded via a nitrogen atom and which is optionally substituted up to 3 times identically or differently by amino, azido, formyl, mercaptyl, carboxyl, hydroxyl, straight-chain or branched acyl. alkoxy, alkylthio or alkoxycarbonyl each having up to 6 carbon atoms, nitro, cyano, halogen, phenyl or straight-chain or branched alkyl having up to 6 carbon atoms, which for its part can be substituted by hydroxyl, amino, azido, carboxyl, straight-chain or branched acyl. alkoxy, alkoxycarbonyl or acylamino each having up to 5 carbon atoms or by a radical of the formula -OR4, in which R4 denotes straight-chain or branched acyl having up to 5 carbon atoms or a group of the formula -SiR5R6R7, in which R5, R6 and R7 are identical or different and denote aryl having 6 to 10 carbon atoms or alkyl having up to 6 carbon atoms, and/or is substituted by a radical of the formula -S(O)c-NR9R10, in which a, b and b' are identical or different and denote a number 0, 1, 2 or 3, R8 denotes hydrogen or straight-chain or branched alkyl having up to 4 carbon atoms, c denotes a number 1 or 2 and R9 and R10 are identical or different and denote hydrogen or straight-chain or branched alkyl having up to 10 carbon atoms, which can optionally be substituted by cycloalkyl having 3 to 8 carbon atoms or by aryl having 6 to carbon atoms, which for its part can be substituted by halogen, or denote aryl having 6 to 10 carbon atoms, which is optionally substituted by halogen, or denote cycloalkyl having 3 to 7 carbon atoms, or R9 and R10, together with the nitrogen atom, form a 5- to 7-membered saturated heterocycle which can optionally contain a further oxygen atom or a radical -NR11, in which R11 denotes hydrogen, straight-chain or branched alkyl having up to 4 carbon atoms or a radical of the formula or denotes benzyl or phenyl, where the ring systems are optionally substituted by halogen, R2 and R3, including the double bond, form a 6-membered saturated or aromatic heterocycle having up to 3 heteroatoms from the group consisting of N, S and/or O, which is optionally substituted up to 3 times identically or differently by formyl, carboxyl, hydroxyl, mercaptyl, straight-chain or branched acyl, alkylthio or alkoxycarbonyl each having up to 6 carbon atoms, nitro, cyano, halogen or straight-chain or branched alkyl or alkoxy each having up to 6 carbon atoms, which for its part can be substituted by hydroxyl, amino, carboxyl, straight-chain or branched acyl, alkoxy or alkoxycarbonyl each having up to 5 carbon atoms.
   and/or the heterocycle is optionally substituted by a group of the formula -NR12R13 or -S(O)c'NR9'R10', in which R12 and R13 are identical or different and denote hydrogen or straight-chain or branched alkyl having up to 6 carbon atoms, or R12 denotes hydrogen and R13 denotes formyl c', R9' and R10' have the meaning of c, R9 and R10 indicated above and are identical to or different from these and/or the heterocycle is optionally substituted by phenyl which for its part can be substituted up to 2 times identically or differently by halogen or by straight-chain or branched alkyl or alkoxy each having up to 6 carbon atoms and/or the heterocycle is optionally substituted by a group of the formula -N=CH-NR14R15, in which R14 and R15 are identical or different and denote hydrogen, phenyl or straight-chain or branched alkyl having up to 6 carbon atoms, A represents a 5- or 6-membered aromatic or saturated heterocycle having up to 3 heteroatoms from the group consisting of S, N and/or O or phenyl, each of which is optionally substituted up to 3 times identically or differently by amino, mercaptyl, hydroxyl, formyl, carboxyl, straight-chain or branched acyl, alkylthio, alkyloxyacyl, alkoxy or alkoxycarbonyl each having up to 6 carbon atoms, nitro, cyano, trifluoromethyl, azido, halogen, phenyl or straight-chain or branched alkyl having up to 6 carbon atoms, which for its part can be substituted by hydroxyl, carboxyl, straight-chain or branched acyl, alkoxy or alkoxycarbonyl each having up to 5 carbon atoms, and/or is substituted by a group of the formula -(CO)d-NR16R17, in which d denotes a number 0 or 1, R16 and R17 are identical or different and denote hydrogen, phenyl, benzyl or straight-chain or branched alkyl or acyl each having up to 5 carbon atoms, and their isomeric forms and salts.
2. 2. Compounds of the general formula (I) according to Claim 1, in which R1 represents pyrimidinyl, pyridazinyl, pyridyl, pyrazinyl.
   tetrahydropyranyl, tetrahydrofuranyl, pyrrolyl, furyl, thienyl, imidazolyl, oxazolyl, thiazolyl, 1,2,3-triazolyl, pyrazolyl, oxadiazolyl, thiadiazolyl, isoxazolyl, isothiazolyl, pyranyl or morpholinyl, each of which is optionally substituted up to 3 times identically or differently by amino, formyl, mercaptyl, carboxyl, hydroxyl, straight-chain or branched acyl, alkoxy, alkylthio or alkoxycarbonyl each having up to 5 carbon atoms, nitro, cyano, azido, fluorine, chlorine, bromine, phenyl or straight-chain or branched alkyl having up to 5 carbon atoms, which for its part can be substituted by hydroxyl, amino, azido, carboxyl, straight-chain or branched acyl, alkoxy, alkoxycarbonyl or acylamino each having up to 4 carbon atoms or by a radical of the formula -OR4, in which R4 denotes straight-chain or branched acyl having up to 4 carbon atoms, and/or by a radical of the formula -S(O)c-NR9R10, in which a, b and b' are identical or different and denote a number 0, 1, 2 or 3, R8 denotes hydrogen or straight-chain or branched alkyl having up to 3 carbon atoms, c denotes a number 1 or 2 and R9 and R10 are identical or different and denote hydrogen or straight-chain or branched alkyl having up to 9 carbon atoms, which can optionally be substituted by cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl or naphthyl or phenyl, which for their part can be substituted by fluorine or chlorine, or denote phenyl or naphthyl, each of which is optionally substituted by fluorine or chlorine, or denote cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl or cycloheptyl, or R9 and R10, together with the nitrogen atom, form a morpholine ring or a radical of the formula in which R11 denotes hydrogen, methyl or a radical of the formula or denotes benzyl or phenyl, where the ring systems are optionally substituted by fluorine or chlorine, R2 and R3, including the double bond, form a pyridyl, pyrimidinyl, pyrazinyl or pyridazinyl ring, each of which is optionally substituted up to 3 times identically or differently by formyl, carboxyl, hydroxyl, mercaptyl, straight-chain or branched acyl, alkylthio or alkoxycarbonyl each having up to 5 carbon atoms, nitro, cyano, azido, fluorine, chlorine, bromine or straight-chain or branched alkyl or alkoxy each having up to 5 carbon atoms, which for its part can be substituted by hydroxyl, amino.
   carboxyl, straight-chain or branched acyl, alkoxy or alkoxycarbonyl each having up to 4 carbon atoms, and/or the abovementioned heterocyclic rings are optionally substituted by a group of the formula -NR12R13 or -S(O)c'NR9'R10', in which R12 and R13 are identical or different and denote hydrogen or straight-chain or branched alkyl having up to 4 carbon atoms, or R12 denotes hydrogen and R13 denotes formyl c', R9' and R10' have the meaning of c, R9 and R10 indicated above and are identical to or different from these and/or the abovementioned heterocyclic rings are optionally substituted by phenyl, which for its part can be substituted by fluorine, chlorine, bromine or by straight-chain or branched alkyl or alkoxy each having up to 4 carbon atoms and/or the abovementioned heterocyclic rings are optionally substituted by a group of the formula in which R14 and R15 denote hydrogen or straight-chain or branched alkyl having up to 4 carbon atoms, A represents thienyl, tetrahydropyranyl, tetrahydrofuranyl, phenyl, morpholinyl, pyrimidyl, pyrazinyl, pyridazinyl or pyridyl, each of which is optionally substituted up to 2 times identically or differently by hydroxyl, formyl, carboxyl, straight-chain or branched acyl, alkylthio, alkoxyacyl, alkoxy or alkoxycarbonyl each having up to 4 carbon atoms, fluorine, chlorine, bromine, nitro, cyano, trifluoromethyl or straight-chain or branched alkyl having up to 4 carbon atoms, which for its part can be substituted by hydroxyl, carboxyl, straight-chain or branched acyl, alkoxy or alkoxycarbonyl each having up to 4 carbon atoms, and/or by a group of the formula -(CO)d-NR16R17, in which d denotes a number 0 or 1, R16 and R17 are identical or different and denote hydrogen, phenyl, benzyl or straight-chain or branched alkyl or acyl each having up to 4 carbon atoms, and their isomeric forms and salts.
3. 3. Compounds of the general formula (I) according to Claim 1, in which R1 represents imidazolyl, furyl, pyridyl, pyrrolyl, pyrazinyl, pyrimidyl, isoxazolyl, oxazolyl or thiazolyl, each of which is optionally substituted up to 3 times identically or differently by formyl, fluorine, chlorine, amino, mercaptyl, cyano, straight-chain or branched acyl, alkylthio, alkoxy or alkoxycarbonyl each having up to 4 carbon atoms or straight-chain or branched alkyl having up to 4 carbon atoms, which for its part can be substituted by hydroxyl, carboxyl, amino, azido, straight-chain or branched acyl, alkoxy, alkoxycarbonyl or acylamino each having up to 3 carbon atoms, and/or by a radical of the formula -S(O)c-NR9R10, in which a, b and b' are identical or different and denote a number 0, 1 or 2, R8 denotes hydrogen or methyl, c denotes a number 1 or 2 and R9 and R10 are identical or different and denote hydrogen or straight-chain or branched alkyl having up to 9 carbon atoms, which can optionally be substituted by phenyl or naphthyl. or denote phenyl or naphthyl, each of which is optionally substituted by fluorine or chlorine, or denote cyclopropyl or cycloheptyl, or R9 and R10, together with the nitrogen atom, form a morpholine ring or a radical of the formula in which R11 denotes hydrogen, methyl or a radical of the formula or denotes benzyl or phenyl, where the ring systems are optionally substituted by chlorine, R2 and R3, including the double bond, form a pyridyl, pyrazinyl, pyrimidinyl or pyridazinyl ring, each of which is optionally substituted up to 3 times identically or differently by formyl, mercaptyl, carboxyl, hydroxyl.
   straight-chain or branched acyl, alkoxy, alkylthio or alkoxycarbonyl each having up to 4 carbon atoms, nitro, cyano, fluorine, chlorine or straight-chain or branched alkyl or alkoxy each having up to 3 carbon atoms, which for its part can be substituted by hydroxyl, amino, carboxyl, straight-chain or branched acyl, alkoxy or alkoxycarbonyl each having up to 3 carbon atoms, and/or the heterocyclic rings are optionally substituted by amino, N,N-dimethylamino or by a radical of the formula -NH-CHO or -N=CH-N(CH3)2 and/or by phenyl, which for its part can be substituted by a radical of the formula -O(CH2)2-CH3, A represents tetrahydropyranyl, phenyl, pyrimidyl, thienyl or pyridyl, each of which is optionally substituted up to 2 times identically or differently by formyl, carboxyl, straight-chain or branched acyl, alkylthio.
   alkyloxyacyl, alkoxy or alkoxycarbonyl each having up to 3 carbon atoms, fluorine, chlorine, bromine, nitro, cyano, trifluoromethyl or straight-chain or branched alkyl having up to 3 carbon atoms, which for its part can be substituted by hydroxyl, carboxyl, straight-chain or branched acyl, alkoxy or alkoxycarbonyl each having up to 3 carbon atoms, and their isomeric forms and salts.
4. 4. Process for the preparation of compounds of the general formula (1) according to Claim 1, characterized in that, depending on the various meanings of the heterocycles defined under R2 and R3, either [A] compounds of the general formula (II) R1-D (II) in which R1 has the meaning indicated above, and D represents radicals of the formula in which R18 represents C1-C4-alkyl, are converted by reaction with compounds of the general formula (III) A-CH2-NH-NH2 (III) in which A has the meaning indicated above in inert solvents, if appropriate in the presence of a base, into the compounds of the general formula (IV) or (IVa) in which A and R1 have the meaning indicated above, and, in the case of the compounds of the general formula (IVa), then cyclized with carboxylic acids, nitriles, formamides or guanidinium salts, and, in the case of the compounds of the general formula (IV), cyclized with 1,3-dicarbonyl derivatives, their salts, tautomers, enol ethers or enamines, in the presence of acids and, if appropriate, under microwaves, or [B] in the case where R2 and R3 together form a pyrazine ring, compounds of the general formula (IV) are first converted by nitrosation into the compounds of the general formula (V) in which A and R1 have the meaning indicated above, in a second step, by means of a reduction, the compounds of the general formula (VI) in which A and R1 have the meaning indicated above, are prepared and finally cyclized with 1,2-dicarbonyl compounds, or [C] compounds of the general formula (VII) in which A1, R2 and R3 have the meaning indicated above, and L represents a radical of the formula -SnR19R20R21, ZnR22, iodine, bromine or triflate, in which R19, R20 and R21 are identical or different and denote straight-chain or branched alkyl having up to 4 carbon atoms, and R22 denotes halogen, are reacted with compounds of the general formula (VIII) R1-T (VIII) in which R1 has the meaning indicated above and if L= SnR19R20R21 or ZnR22 T represents triflate or halogen, and if L= iodine, bromine or triflate, T represents a radical of the formula SnR19R20R21, ZnR22 or BR23'R24', in which R19, R20, R21 and R22 have the meaning of R19, R20, R21 and R22 indicated above, R23 and R24 are identical or different and denote hydroxyl.
   
   aryloxy having 6 to 10 carbon atoms or straight-chain or branched alkyl or alkoxy each having up to 5 carbon atoms, or together form a 5- or 6-membered carbocyclic ring, in a palladium-catalysed reaction in inert solvents, if appropriate in the presence of a base, [D] if R1= in which R25 denotes (C1-C6)-alkyl which is optionally substituted by halogen, compounds of the general formula (IX) in which A, R2 and R3 have the meaning indicated above, are converted either directly by reaction with the compound of the formula (X) in which R25 has the meaning indicated above, in the system NaOCO-CH3/N-methylpyrrolidine into the compounds of the general formula (Ia) in which R2, R3 and A and R25 have the meaning indicated above, and then, by action of potassium hydroxide in methanol, the acetyl group is removed, or first, by reaction of the compounds of the general formula (IX) with the compound of the formula (X), the compounds of the general formula (XI) in which R2, R3, A and R25 have the meaning indicated above, are prepared, and in a further step, by action of potassium hydroxide, the hydroxymethyl compounds are prepared, and, if appropriate, converted into the corresponding alkoxy compounds by alkylation according to customary methods, and in the case of the groups -S(O)~ NR9R10 and -S(O)c NR9R10, starting from the unsubstituted compounds of the general formula (I), first reacted with thionyl chloride and in a second step with the appropriate amines and, if appropriate, the substituents mentioned under R1,R2, R3 and/or A are varied or introduced according to customary methods.
5. 5. The process of claim 4, wherein: [B] the cyclization is carried out with an aqueous glyoxal solution;
   wherein [C] T represents bromine; and wherein the substituents mentioned under R1, R2, R3 and/or A are varied or introduced by chlorination, catalytic hydrogenation, reduction, oxidation, removal of protective groups and/or nucleophilic substitution.
6. 6. A pharmaceutical composition comprising at least one compound as defined in any one of claims 1 to 3, or an isomeric form thereof or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient.
7. 7. The composition of claim 4, further comprising an organic nitrate or NO donor.
8. 8. The composition of claim 4, further comprising a compound which inhibits the degradation of cyclic guanosine monophosphate (cGMP).
9. 9. Use of a compound as defined in any one of claims 1 to 3, or a composition as defined in any one of claims 6 to 8, in the production of a medicament for the treatment of a cardiovascular disorder.
10. 10. Use of a compound as defined in any one of claims 1 to 3, or a composition as defined in any one of claims 6 to 8, in the production of a medicament for the treatment of a thromboembolic disorder and an ischaemia.
11. 11. Use of a compound as defined in any one of claims 1 to 3, or a composition as defined in any one of claims 6 to 8, for the treatment of a cardiovascular disorder.
12. 12. Use of a compound as defined in any one of claims 1 to 3, or a composition as defined in any one of claims 6 to 8, for the treatment of a thromboembolic disorder and an ischaemia.
13. 13. A commercial package comprising a compound as defined in any one of claims 1 to 3, or a composition as defined in any one of claims 6 to 8, and associated therewith instructions for the use thereof in the treatment of a cardiovascular disorder.
14. 14. A commercial package comprising a compound as defined in any one of claims 1 to 3, or a composition as defined in any one of claims 6 to 8, and associated therewith instructions for the use thereof in the treatment of a thromboembolic disorder and an ischaemia.