CA2267999A1 - Procede tres sensible de detection sur de faibles volumes des atteintes a l'environnement - Google Patents

Procede tres sensible de detection sur de faibles volumes des atteintes a l'environnement Download PDF

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Publication number
CA2267999A1
CA2267999A1 CA002267999A CA2267999A CA2267999A1 CA 2267999 A1 CA2267999 A1 CA 2267999A1 CA 002267999 A CA002267999 A CA 002267999A CA 2267999 A CA2267999 A CA 2267999A CA 2267999 A1 CA2267999 A1 CA 2267999A1
Authority
CA
Canada
Prior art keywords
stress
detector
prokaryote
alters
promoter
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002267999A
Other languages
English (en)
Inventor
Robert Alan Larossa
Pablo Ariel Scolnik
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2267999A1 publication Critical patent/CA2267999A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Il est possible de détecter et mesurer d'infimes variations des contraintes environnementales à des niveaux sublétaux sous forme d'une réponse générique auxdites contraintes. L'invention porte sur un procédé amélioré de détection des susdites variation dans de très faibles volumes, ce qui permet des rendements d'analyse très élevés. Les variations des contraintes sont mises en évidence par des modifications de la luminescence d'un micro-organisme produit par génie génétique. Le complexe de gènes luminescents est commandé par un promoteur réagissant aux contraintes. Les volumes prévus vont d'environ 0,5 uL à environ 10 mL du milieu dans lequel le micro-organisme détecteur est en suspension.
CA002267999A 1996-11-15 1997-11-14 Procede tres sensible de detection sur de faibles volumes des atteintes a l'environnement Abandoned CA2267999A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US3127296P 1996-11-15 1996-11-15
US60/031,272 1996-11-15
PCT/US1997/020793 WO1998021347A1 (fr) 1996-11-15 1997-11-14 Procede tres sensible de detection sur de faibles volumes des atteintes a l'environnement

Publications (1)

Publication Number Publication Date
CA2267999A1 true CA2267999A1 (fr) 1998-05-22

Family

ID=21858542

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002267999A Abandoned CA2267999A1 (fr) 1996-11-15 1997-11-14 Procede tres sensible de detection sur de faibles volumes des atteintes a l'environnement

Country Status (4)

Country Link
EP (1) EP0943000A1 (fr)
JP (1) JP2001503990A (fr)
CA (1) CA2267999A1 (fr)
WO (1) WO1998021347A1 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001514501A (ja) * 1997-02-28 2001-09-11 イー・アイ・デユポン・ドウ・ヌムール・アンド・カンパニー 生体異物化学品の作用部位の同定のための方法
KR100414784B1 (ko) * 2000-01-18 2004-01-13 (주)바이오니아 연속식 수질 독성 검사 장치
GB2364307A (en) * 2000-04-03 2002-01-23 Univ Surrey Bioluminescent reporters
US20030108980A1 (en) * 2000-08-14 2003-06-12 Sayler Gary S. Bioluminescent methods for direct visual detection of environmental compounds
JP2002335979A (ja) 2000-12-22 2002-11-26 Pfizer Prod Inc 新規生物発光アッセイ及びそれに有用な細菌株
WO2004033421A2 (fr) 2002-10-04 2004-04-22 Genencor International, Inc. Production amelioree de souches bacteriennes
CN100334431C (zh) * 2003-02-20 2007-08-29 华为技术有限公司 一种环境应力实验自动测试方法
WO2004076685A2 (fr) * 2003-02-28 2004-09-10 Lux Biotechnology Limited Biocapteur et bioanalyse fongique
JP2008035819A (ja) * 2006-08-09 2008-02-21 Daikin Ind Ltd プロモーターアッセイ方法に用いる容器、該容器を含むプレートおよびそれらを用いるプロモーターアッセイ方法
US20140051101A1 (en) * 2012-08-20 2014-02-20 Carnegie Institution Of Washington Luciferase Reporter System for Roots and Methods of Using the Same

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5589337A (en) * 1992-07-06 1996-12-31 The President And Fellows Of Harvard College Methods and diagnostic kits for determining toxicity utilizing bacterial stress promoters fused to reporter genes
IL107815A (en) * 1992-12-04 2003-12-10 Du Pont Genetic constructs comprising a stress-responsive promoter linked to a lux reporter operon and methods of use in environmental testing
US5702883A (en) * 1993-10-22 1997-12-30 Kabushiki Kaisha Toyota Chuo Kenkyusho Methods for detection of mutagens using luminescence gene
CA2200702C (fr) * 1994-11-23 2000-12-05 E.I. Du Pont De Nemours And Company Reactif bacterien bioluminescent lyophilise servant a la detection d'agents toxiques

Also Published As

Publication number Publication date
EP0943000A1 (fr) 1999-09-22
WO1998021347A1 (fr) 1998-05-22
JP2001503990A (ja) 2001-03-27

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