CA2264961A1 - Appareil et procede pour la mise en oeuvre d'un sequencage de polymeres nucleotidiques - Google Patents

Appareil et procede pour la mise en oeuvre d'un sequencage de polymeres nucleotidiques Download PDF

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Publication number
CA2264961A1
CA2264961A1 CA002264961A CA2264961A CA2264961A1 CA 2264961 A1 CA2264961 A1 CA 2264961A1 CA 002264961 A CA002264961 A CA 002264961A CA 2264961 A CA2264961 A CA 2264961A CA 2264961 A1 CA2264961 A1 CA 2264961A1
Authority
CA
Canada
Prior art keywords
region
sequencing
dna
thermocycling
fragments
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002264961A
Other languages
English (en)
Inventor
Paul Waterhouse
Alexandre M. Izmailov
Henryk Zaleski
Thomas D. Yager
James M. Dunn
James Leushner
May Hui
Marina T. Larson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Visible Genetics Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2264961A1 publication Critical patent/CA2264961A1/fr
Abandoned legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44743—Introducing samples

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Cette invention concerne un appareil conçu pour traiter des échantillons contenant de l'ADN en vue de la production d'un mélange de fragments de séquençage. Ledit appareil comporte un élément de traitement des échantillons possédant une région de thermocyclage comprenant une ou plusieurs chambres conçues pour recevoir un mélange réactionnel de séquençage d'ADN et pour former à partir dudit mélange des fragments de séquençage, une région de séparation comportant une matrice servant à séparer les fragments de séquençage formés dans la région de thermocyclage, une région de détection permettant la détection des fragments de séquençage séparés, et un organe de régulation de la température, disposé dans la région de thermocyclage de l'élément de traitement des échantillons, servant à produire une pluralité de cycles thermiques, chacun de ces cycles comportant au moins une phase de dénaturation et une phase d'extension. On peut placer ledit appareil de traitement des échantillons dans un support associé d'une part, à un organe servant à appliquer un champ électrique à la région de séparation de l'appareil de traitement d'échantillons placé à l'intérieur dudit support de manière à provoquer la migration des fragments de séquençage des polynucléotides à travers la région de séparation, de la région de thermocyclage vers la région de détection, et d'autre part à un organe de détection des fragments de polynucléotides au sein de la région de détection de l'appareil de traitement des échantillons placé à l'intérieur dudit support.
CA002264961A 1996-08-27 1997-08-27 Appareil et procede pour la mise en oeuvre d'un sequencage de polymeres nucleotidiques Abandoned CA2264961A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US70371096A 1996-08-27 1996-08-27
US08/703,710 1996-08-27
PCT/US1997/015056 WO1998008978A1 (fr) 1996-08-27 1997-08-27 Appareil et procede pour la mise en oeuvre d'un sequençage de polymeres nucleotidiques

Publications (1)

Publication Number Publication Date
CA2264961A1 true CA2264961A1 (fr) 1998-03-05

Family

ID=24826465

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002264961A Abandoned CA2264961A1 (fr) 1996-08-27 1997-08-27 Appareil et procede pour la mise en oeuvre d'un sequencage de polymeres nucleotidiques

Country Status (4)

Country Link
EP (1) EP0927268A1 (fr)
AU (1) AU4236597A (fr)
CA (1) CA2264961A1 (fr)
WO (1) WO1998008978A1 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
FR2795426A1 (fr) * 1999-06-22 2000-12-29 Commissariat Energie Atomique Support d'analyse biologique a amplification
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
CA2557177A1 (fr) 2004-02-19 2005-09-01 Stephen Quake Procedes et kits pour analyser des sequences de polynucleotides
IL160869A0 (en) 2004-03-15 2004-08-31 Dnr Imaging System Ltd Integrated system for real time gel electrophoresis running and documenting
US7223949B2 (en) 2004-04-21 2007-05-29 Beckman Coulter, Inc. Analysis apparatus having improved temperature control unit
EP1642648A1 (fr) * 2004-09-30 2006-04-05 Roche Diagnostics GmbH Dispositif et proeédé pour regler la temperature d'un liquide
EP1642647A1 (fr) * 2004-09-30 2006-04-05 Roche Diagnostics GmbH Dispositif et proeédé pour regler la temperature d'un liquide
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5188963A (en) * 1989-11-17 1993-02-23 Gene Tec Corporation Device for processing biological specimens for analysis of nucleic acids
DE8814398U1 (fr) * 1988-11-17 1989-02-16 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften Ev, 3400 Goettingen, De
US5217591A (en) * 1990-05-14 1993-06-08 Labintelligence, Inc. Gel electrophoresis sample applicator/retriever
EP0595982A4 (en) * 1991-07-24 1995-10-11 Univ Partnerships Pty Ltd Single step amplification and sequencing of nucleic acids
CA2134476C (fr) * 1992-05-01 1999-08-10 Peter Wilding Manipulation de liquides dans des dispositifs d'analyse microfabriques
JPH05332992A (ja) * 1992-05-29 1993-12-17 Shimadzu Corp 電気泳動装置
US5639423A (en) * 1992-08-31 1997-06-17 The Regents Of The University Of Calfornia Microfabricated reactor
US5710628A (en) * 1994-12-12 1998-01-20 Visible Genetics Inc. Automated electrophoresis and fluorescence detection apparatus and method
US6017434A (en) * 1995-05-09 2000-01-25 Curagen Corporation Apparatus and method for the generation, separation, detection, and recognition of biopolymer fragments
AU2816897A (en) * 1996-05-01 1997-11-19 Visible Genetics Inc. Method for sequencing of nucleic acid polymers

Also Published As

Publication number Publication date
WO1998008978A1 (fr) 1998-03-05
AU4236597A (en) 1998-03-19
EP0927268A1 (fr) 1999-07-07

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