EP1642647A1 - Dispositif et proeédé pour regler la temperature d'un liquide - Google Patents

Dispositif et proeédé pour regler la temperature d'un liquide Download PDF

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Publication number
EP1642647A1
EP1642647A1 EP04023309A EP04023309A EP1642647A1 EP 1642647 A1 EP1642647 A1 EP 1642647A1 EP 04023309 A EP04023309 A EP 04023309A EP 04023309 A EP04023309 A EP 04023309A EP 1642647 A1 EP1642647 A1 EP 1642647A1
Authority
EP
European Patent Office
Prior art keywords
temperature
liquid
control unit
sample vessels
sensor element
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP04023309A
Other languages
German (de)
English (en)
Inventor
Roger Sandoz
Frank Ulrich Schubert
Hans-Rudolf Bachmann
Renato Baumann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Roche Diagnostics GmbH
Original Assignee
F Hoffmann La Roche AG
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG, Roche Diagnostics GmbH filed Critical F Hoffmann La Roche AG
Priority to EP04023309A priority Critical patent/EP1642647A1/fr
Priority to EP05017580A priority patent/EP1642648A1/fr
Priority to CA002516885A priority patent/CA2516885C/fr
Priority to US11/226,818 priority patent/US7600438B2/en
Priority to JP2005286954A priority patent/JP4885506B2/ja
Publication of EP1642647A1 publication Critical patent/EP1642647A1/fr
Priority to US12/553,827 priority patent/US20090320617A1/en
Withdrawn legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/143—Quality control, feedback systems
    • B01L2200/147—Employing temperature sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1861—Means for temperature control using radiation

Definitions

  • the present invention relates to a device for adjusting a temperature of a liquid according to the preamble of claim 1 and a corresponding method.
  • the present invention is therefore based on the object of specifying a device for adjusting a temperature of a liquid, wherein the device does not have one or more of the disadvantages mentioned above.
  • the invention has the following advantages: Since the liquid to be examined contains absorption elements, the temperature setting in the liquid to be examined is considerably accelerated. Thus, the throughput of samples per unit time can be increased accordingly.
  • Fig. 1 shows a schematic representation of an embodiment of the invention, in which eight sample vessels 11 to 18 are arranged substantially on a straight line, wherein a transport unit 20 is provided to hold one hand, the sample vessels 11 to 18 in position and on the other hand, to a to ensure easy transport of the sample vessels 11 to 18.
  • a temperature control unit 2 is provided laterally along the sample vessels 11 to 18 or the transport unit 20, by means of which the temperature of the liquid present in the sample vessels 11 to 18 can be adjusted.
  • a control unit 1 is provided, which is operatively connected to the temperature control unit 2, ie in the control unit 1, a control signal is generated, which leads to a corresponding temperature radiation through the temperature control unit 2.
  • a first embodiment of the present invention is that the control unit 1 receives no feedback on the temperature generated in the sample vessels 11 to 18.
  • a further embodiment of the present invention is that sensor elements 3 are provided in the region of the sample vessels 11 to 18, by means of which the respective temperature of the existing in the sample vessels 11 to 18 fluids can be determined.
  • sensor elements 3 are provided in the region of the sample vessels 11 to 18, by means of which the respective temperature of the existing in the sample vessels 11 to 18 fluids can be determined.
  • the possibility that for each sample vessel 11 to 18 a sensor element 3 is present on the other hand the possibility that the temperature is measured only in one of the sample vessels 11 to 18, in which case it is assumed that the measured temperature value in all other sample vessels 11 to 18 is the same.
  • the embodiments of the present invention with sensor elements 3 enables the regulation of the temperature radiation of the temperature control unit 2, whereby a desired temperature of the liquids present in the sample vessels can be adjusted quickly and precisely.
  • FIG. 1 denoted by 5 is a system bus, via which the device according to the invention can be coupled, for example, to a higher-level system which assumes, for example, all the controls of a process.
  • an IR radiation unit is particularly suitable as tempering unit 2.
  • An IR radiation unit irradiates the liquid in the sample vessels 11 to 18 in the infrared wavelength range.
  • other wavelength ranges are also conceivable.
  • the tempering unit 2 used is realized, for example, as a surface radiator (two-dimensional) in thick-film or thin-film technology.
  • the absorption elements have the task to absorb the radiation energy emitted by the temperature control unit 2 and deliver it as heat to the liquids contained in the sample vessels 11 to 18.
  • the choice for an absorption element is therefore dependent on the temperature control unit 2 used or on the wavelength range of the radiation used.
  • absorption elements are, for example, spherical particles in the size of 0.5 to 5 microns. These are glass beads with enclosed magnetic pigments, for example iron oxide. Such absorption elements are also referred to as MGPs (Magnetic Glass Particles). Furthermore, the absorption can be increased by the use of polymers (PS) for the production of absorbent elements. Finally, the thermal conductivity and thus a Heat input into the liquids can be increased by absorption elements of other inert particles (for example, aluminum, ceramic or carbon fibers) are added.
  • PS Polymers
  • Particularly suitable absorption elements are particulate solids, as described, for example, in the known teachings of WO 96/41 811 or WO 00/32 762 or WO 01/37 291 of the same Applicant.
  • the disclosure of the above-mentioned international patent applications is therefore fully an integral part of the present patent application.
  • the absorption elements primarily have the task of converting radiation into heat and delivering it to the liquid to be heated in the sample vessel in order to be able to achieve a desired temperature of the liquid as quickly as possible.
  • embodiments are conceivable and desirable in which particles are used as absorption elements, to which nucleic acid can be reversibly bound, as has also been described in the aforementioned international patent application with the publication number WO 96/41 811.
  • the method consists of binding nucleic acids to the particles for cleaning. Through the connection, an extremely efficient heat transfer can be obtained.
  • the liquid to be examined is preferably aqueous, in particular a nucleic acid-containing Sample, for example, a body fluid or a liquid derived therefrom.
  • sample vessels 11 to 18 are made of a material with low heat capacity and / or reduced absorption.
  • COC cycloolefin copolymer
  • PP polypropylene
  • the measurement of the instantaneous temperature by means of the sensor elements 3 is preferably, but not necessarily, from above, that is on the Opening in the sample vessels 11 to 18. This allows a direct measurement of the temperature to be made, and there are none Messvertigschept due to lying between the sensor element 3 and the liquid vessel walls to be expected.
  • the liquid in the sample vessels 11 to 18 can be heated from below or from above. In this case, a temperature measurement from the side is preferred.
  • FIG. 2 shows a further embodiment of the device according to the invention with a linear IR incubator.
  • the embodiment according to FIG. 2 comprises a rake-shaped tempering unit, which consists of the essentially parallel tempering elements 2a to 2f.
  • the tempering elements 2a to 2f can also be produced with the mentioned thin film or thick film technologies.
  • the control unit 1 is individually connected to the temperature control 2a to 2f.
  • the temperature measurement takes place, as in the embodiment according to FIG. 1, via sensor elements 3, which are connected to the control unit 1 (shown in dashed lines in FIG. 2).
  • the sensor elements 3 are arranged above or below the sample vessels 11 to 15.
  • sensor elements 3 ' are provided directly on the temperature control elements 2a to 2f, as is indicated by way of example in the case of the first temperature control element 2a.
  • Fig. 3 shows a further embodiment of the inventive device is shown.
  • a so-called rotor-IR incubator is used, in which the sample vessels 11 to 18 are arranged on a circle. Accordingly, the sample vessels 11 to 18 are held in position by a circular transport unit 20.
  • the tempering unit 2 is arranged in the center of the circular transport unit 20, so that the heat rays run radially, thus impinging laterally on the sample vessels 11 to 18.
  • a single or multiple sensor elements 3 are also provided in that according to FIG. 3 in order to measure the temperature of the liquids contained in the sample vessels 11 to 18 and optionally to the control unit 1 for regulating the temperature to pass over the temperature control unit 2.
  • the sensor unit or the sensor units are to be suitably placed.
  • an arrangement is suitable the sensor unit 3 above the sample vessels 11 to 18, whereby a direct influence by the temperature control unit 2 is excluded.
  • FIG. 4 shows a further embodiment of the device according to the invention with a rotor-IR-incubator.
  • the embodiment according to FIG. 4 consists in that the temperature control unit 2 is arranged below one of the sample vessels 11 to 18. Conceivable and in a modification of the embodiment according to FIG. 4, a further embodiment variant is that a temperature control unit 2 is arranged under a plurality of or among all sample vessels 11 to 18.
  • a water temperature of 80 ° Celsius was reached after about 40 seconds.
  • the sample vessel is placed concentrically over a halogen lamp as a tempering unit, wherein the halogen lamp is arranged in a rotationally symmetrical mirror.
  • a wavelength filter is further provided between the tempering unit and the sample vessel.
  • a sensor element is provided as a non-contact temperature sensor, with which the control unit and the temperature control unit are operatively connected.
  • tempering unit 2 which generates beams in the infrared range, is particularly suitable for all of the described embodiments according to the invention. Nevertheless, tempering units are also conceivable which generate beams in other wavelength ranges. Decisive is a vote of the used rays in connection with the used materials for the absorption elements and for the sample vessels 11 to 18.
  • Suitable sample vessels are conventional so-called tubes, which consist of a cylindrical section and leak towards the closed end, for example, in a tip.
  • so-called flat cells are suitable, which consist essentially of one or more chambers with a small depth (a few hundred microns) in a carrier material, wherein the outer dimensions of the chamber and its geometry may be arbitrary.
  • hollow cylinders and capillary tubes are also suitable as sample vessels.
  • the present invention is particularly suitable for the following applications: incubators, thermal cyclers and all applications related to energy input.

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  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
EP04023309A 2004-09-30 2004-09-30 Dispositif et proeédé pour regler la temperature d'un liquide Withdrawn EP1642647A1 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP04023309A EP1642647A1 (fr) 2004-09-30 2004-09-30 Dispositif et proeédé pour regler la temperature d'un liquide
EP05017580A EP1642648A1 (fr) 2004-09-30 2005-08-12 Dispositif et proeédé pour regler la temperature d'un liquide
CA002516885A CA2516885C (fr) 2004-09-30 2005-08-24 Dispositif et methode de reglage de la temperature d'un liquide
US11/226,818 US7600438B2 (en) 2004-09-30 2005-09-13 Device and method for the adjustment of a temperature of a liquid
JP2005286954A JP4885506B2 (ja) 2004-09-30 2005-09-30 液体の温度を調節する装置および方法
US12/553,827 US20090320617A1 (en) 2004-09-30 2009-09-03 Device and Method for the Adjustment of a Temperature of a Liquid

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP04023309A EP1642647A1 (fr) 2004-09-30 2004-09-30 Dispositif et proeédé pour regler la temperature d'un liquide

Publications (1)

Publication Number Publication Date
EP1642647A1 true EP1642647A1 (fr) 2006-04-05

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ID=34926794

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EP04023309A Withdrawn EP1642647A1 (fr) 2004-09-30 2004-09-30 Dispositif et proeédé pour regler la temperature d'un liquide

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EP (1) EP1642647A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2514614A (en) * 2013-05-31 2014-12-03 Simon Johnson Chemical process apparatus and methods

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996041864A1 (fr) * 1995-06-13 1996-12-27 The Regents Of The University Of California Chambre de microreaction chauffee par laser a diode dotee d'un moyen de detection d'echantillons
US5721123A (en) * 1996-01-05 1998-02-24 Microfab Technology, Inc. Methods and apparatus for direct heating of biological material
WO1998008978A1 (fr) * 1996-08-27 1998-03-05 Visible Genetics Inc. Appareil et procede pour la mise en oeuvre d'un sequençage de polymeres nucleotidiques

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996041864A1 (fr) * 1995-06-13 1996-12-27 The Regents Of The University Of California Chambre de microreaction chauffee par laser a diode dotee d'un moyen de detection d'echantillons
US5721123A (en) * 1996-01-05 1998-02-24 Microfab Technology, Inc. Methods and apparatus for direct heating of biological material
WO1998008978A1 (fr) * 1996-08-27 1998-03-05 Visible Genetics Inc. Appareil et procede pour la mise en oeuvre d'un sequençage de polymeres nucleotidiques

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2514614A (en) * 2013-05-31 2014-12-03 Simon Johnson Chemical process apparatus and methods

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