CA2245004C - Dispositif d'amorcage de la congelation dans les systemes de conservation cryogenique - Google Patents

Dispositif d'amorcage de la congelation dans les systemes de conservation cryogenique Download PDF

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Publication number
CA2245004C
CA2245004C CA 2245004 CA2245004A CA2245004C CA 2245004 C CA2245004 C CA 2245004C CA 2245004 CA2245004 CA 2245004 CA 2245004 A CA2245004 A CA 2245004A CA 2245004 C CA2245004 C CA 2245004C
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Canada
Prior art keywords
rack
fluid
ice
containers
sprayrail
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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CA 2245004
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English (en)
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CA2245004A1 (fr
Inventor
Maury D. Cosman
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Organogenesis Inc
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Organogenesis Inc
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Publication date
Priority claimed from US08/593,587 external-priority patent/US5689961A/en
Application filed by Organogenesis Inc filed Critical Organogenesis Inc
Publication of CA2245004A1 publication Critical patent/CA2245004A1/fr
Application granted granted Critical
Publication of CA2245004C publication Critical patent/CA2245004C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Dispositif d'amorçage de la congélation pour conservation cryogénique d'échantillons biologiques tels que des cellules, des tissus prélevés et des constructions biologiques cellulaires telles que des équivalents de tissus de culture. Avec ce dispositif, l'amorçage de la congélation s'effectue en libérant un gaz liquifié ou refroidi (60), à partir d'une buse (21), sur la surface d'un contenant (40) abritant ledit échantillon biologique à conserver par le froid. A l'intérieur d'une chambre de congélation (70), les contenants (40) sont placés sur des grilles (30) disposées à proximité d'une rampe de diffusion (20) comportant des buses (21). L'amorçage de la congélation s'effectue par libération d'un gaz liquéfié ou refroidi (60) à partir de ces buses (21) en direction de la surface du contenant, à la température d'équilibre solide/liquide du conservateur cryogénique. Le refroidissement localisé de la surface du contenant, effectué par cette libération, entraîne la formation de glace à l'intérieur du contenant.
CA 2245004 1996-01-30 1997-01-30 Dispositif d'amorcage de la congelation dans les systemes de conservation cryogenique Expired - Lifetime CA2245004C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/593,587 US5689961A (en) 1996-01-30 1996-01-30 Ice seeding apparatus for cryopreservation systems
US08/593,587 1996-01-30
PCT/US1997/001619 WO1997028402A1 (fr) 1996-01-30 1997-01-30 Dispositif d'amorçage de la congelation dans les systemes de conservation cryogenique

Publications (2)

Publication Number Publication Date
CA2245004A1 CA2245004A1 (fr) 1997-08-07
CA2245004C true CA2245004C (fr) 2008-01-15

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ID=29422877

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Application Number Title Priority Date Filing Date
CA 2245004 Expired - Lifetime CA2245004C (fr) 1996-01-30 1997-01-30 Dispositif d'amorcage de la congelation dans les systemes de conservation cryogenique

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CA (1) CA2245004C (fr)

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Publication number Publication date
CA2245004A1 (fr) 1997-08-07

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