CA2241849A1 - Proteines de fusion comprenant des proteines regulatrices du cycle cellulaire - Google Patents

Proteines de fusion comprenant des proteines regulatrices du cycle cellulaire Download PDF

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Publication number
CA2241849A1
CA2241849A1 CA 2241849 CA2241849A CA2241849A1 CA 2241849 A1 CA2241849 A1 CA 2241849A1 CA 2241849 CA2241849 CA 2241849 CA 2241849 A CA2241849 A CA 2241849A CA 2241849 A1 CA2241849 A1 CA 2241849A1
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CA
Canada
Prior art keywords
fusion proteins
purification
cyclin
seq
human
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2241849
Other languages
English (en)
Inventor
Steven Kovacevic
Keith Allen Otto
Ramachandra Nagaraja Rao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eli Lilly and Co
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2241849A1 publication Critical patent/CA2241849A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4738—Cell cycle regulated proteins, e.g. cyclin, CDC, INK-CCR
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/026—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a baculovirus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne de nouvelles protéines de fusion comprenant des cyclines et des CDK. Une réalisation préférée de l'invention porte sur des protéines de fusion comprenant de la cycline D1 humaine et de la CDK4 humaine. Les protéines de l'invention présentent facultativement des modifications qui en facilitent la purification. L'addition de restes histidine à des produits de recombinaison sélectionnés permet une purification par chromatographie d'affinité à métaux immobilisés. Certaines réalisations de l'invention concernent des déterminants antigéniques permettant la purification par chromatographie d'affinité aux anticorps monoclonaux. Des sites de clivage de protéase sont incorporés dans les produits de recombinaison sélectionnés pour permettre le clivage des régions incorporées dans les protéines de fusion cycline-CDK destinées à la purification. Des modifications supplémentaires facilitant la purification concernent des domaines de liaison de la strépavadine et des déterminants antigéniques destinés à la chromatographie d'affinité aux anticorps.
CA 2241849 1996-01-05 1997-01-03 Proteines de fusion comprenant des proteines regulatrices du cycle cellulaire Abandoned CA2241849A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US962996P 1996-01-05 1996-01-05
US60/009,629 1996-01-05

Publications (1)

Publication Number Publication Date
CA2241849A1 true CA2241849A1 (fr) 1997-07-17

Family

ID=21738815

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2241849 Abandoned CA2241849A1 (fr) 1996-01-05 1997-01-03 Proteines de fusion comprenant des proteines regulatrices du cycle cellulaire

Country Status (4)

Country Link
EP (1) EP0871659A4 (fr)
AU (1) AU1526897A (fr)
CA (1) CA2241849A1 (fr)
WO (1) WO1997025345A1 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5986055A (en) * 1997-11-13 1999-11-16 Curagen Corporation CDK2 interactions
US6432668B1 (en) 1997-12-30 2002-08-13 Chiron Corporation Polynucleotides encoding human cyclin-dependent kinase (hPFTAIRE)
KR20000019889A (ko) * 1998-09-16 2000-04-15 성재갑 곤충세포를 이용한 cdk4/p16 복합체의 제조방법
JP2003524389A (ja) 1998-12-16 2003-08-19 カイロン コーポレイション ヒトサイクリン依存的キナーゼ（ｈＰＮＱＡＬＲＥ）
US6716626B1 (en) 1999-11-18 2004-04-06 Chiron Corporation Human FGF-21 nucleic acids
DE60043197D1 (fr) 1999-11-18 2009-12-03 Univ Kyoto
US20020082205A1 (en) 2000-03-08 2002-06-27 Nobuyuki Itoh Human FGF-23 gene and gene expression products
DE10113776B4 (de) 2001-03-21 2012-08-09 "Iba Gmbh" Isoliertes streptavidinbindendes, kompetitiv eluierbares Peptid, dieses umfassendes Fusionspeptid, dafür codierende Nukleinsäure, Expressionsvektor, Verfahren zur Herstellung eines rekombinanten Fusionsproteins und Verfahren zum Nachweis und/oder zur Gewinnung des Fusionsproteins
US20150299764A1 (en) * 2012-09-05 2015-10-22 Universite Libre De Bruxelles Immobilised cyclin-dependent kinase 4 fusion proteins and uses thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5196320A (en) * 1989-09-20 1993-03-23 Abbott Biotech, Inc. Method of producing engineered binding proteins

Also Published As

Publication number Publication date
EP0871659A4 (fr) 2000-04-26
WO1997025345A1 (fr) 1997-07-17
EP0871659A1 (fr) 1998-10-21
AU1526897A (en) 1997-08-01

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