CA2232233A1 - Identification of, and uses for, nematicidal bacillus thuringiensis genes, toxins, and isolates - Google Patents

Identification of, and uses for, nematicidal bacillus thuringiensis genes, toxins, and isolates Download PDF

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Publication number
CA2232233A1
CA2232233A1 CA002232233A CA2232233A CA2232233A1 CA 2232233 A1 CA2232233 A1 CA 2232233A1 CA 002232233 A CA002232233 A CA 002232233A CA 2232233 A CA2232233 A CA 2232233A CA 2232233 A1 CA2232233 A1 CA 2232233A1
Authority
CA
Canada
Prior art keywords
toxin
nematicidal
isolate
nrrl
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002232233A
Other languages
English (en)
French (fr)
Inventor
Jerald S. Feitelson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mycogen Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/540,104 external-priority patent/US5667993A/en
Application filed by Individual filed Critical Individual
Publication of CA2232233A1 publication Critical patent/CA2232233A1/en
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
CA002232233A 1995-10-06 1996-10-01 Identification of, and uses for, nematicidal bacillus thuringiensis genes, toxins, and isolates Abandoned CA2232233A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US08/540,104 US5667993A (en) 1995-10-06 1995-10-06 Primers and probes for the identification of bacillus thuringiensis genes and isolates
US540,104 1995-10-06
US08/620,717 US5670365A (en) 1995-10-06 1996-03-21 Identification of, and uses for, nematicidal bacillus thuringiensis genes, toxins, and isolates
US620,717 1996-03-21
PCT/US1996/015730 WO1997012980A1 (en) 1995-10-06 1996-10-01 Identification of, and uses for, nematicidal bacillus thuringiensis genes, toxins, and isolates

Publications (1)

Publication Number Publication Date
CA2232233A1 true CA2232233A1 (en) 1997-04-10

Family

ID=27066327

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002232233A Abandoned CA2232233A1 (en) 1995-10-06 1996-10-01 Identification of, and uses for, nematicidal bacillus thuringiensis genes, toxins, and isolates

Country Status (10)

Country Link
US (1) US5670365A (https=)
EP (1) EP0853671B1 (https=)
JP (1) JP4359653B2 (https=)
AT (1) ATE287955T1 (https=)
AU (1) AU729343C (https=)
CA (1) CA2232233A1 (https=)
DE (1) DE69634258T2 (https=)
ES (1) ES2235198T3 (https=)
NZ (1) NZ319495A (https=)
WO (1) WO1997012980A1 (https=)

Families Citing this family (22)

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Publication number Priority date Publication date Assignee Title
US6124359A (en) * 1995-10-20 2000-09-26 Mycogen Corporation Materials and methods for killing nematodes and nematode eggs
US6242669B1 (en) 1996-10-30 2001-06-05 Mycogen Corporation Pesticidal toxins and nucleotide sequences which encode these toxins
US7129212B2 (en) * 1996-10-30 2006-10-31 Mycogen Corporation Polynucleotides, pesticidal proteins, and novel methods of using them
AU5098398A (en) 1996-10-30 1998-05-22 Mycogen Corporation Novel pesticidal toxins and nucleotide sequences which encode these toxins
US6603063B1 (en) * 1999-05-07 2003-08-05 Mycogen Corp. Plants and cells transformed with a nucleic acid from Bacillus thuringiensis strain KB59A4-6 encoding a novel SUP toxin
US5986177A (en) * 1997-01-10 1999-11-16 Agricultural Genetic Engineering Research Institute Bacillus thuringiensis isolates with broad spectrum activity
AR012067A1 (es) * 1997-03-13 2000-09-27 Mycogen Corp Metodo para controlar una plaga vegetal con una toxina de bacillus thuringiensis, cultivo biologicamente puro, secuencia de polinucleotido quecodifica una toxina pesticida y toxina pesticida de un b.t. aislado
WO1998059067A1 (en) * 1997-06-20 1998-12-30 Mycogen Corporation Method to identify pesticidal microbes
US5973231A (en) 1998-05-12 1999-10-26 Mycogen Corporation Bacillus thuringiensis isolates, toxins, and genes for controlling certain coleopteran pests
US6887900B2 (en) 2002-03-04 2005-05-03 Divergence, Inc. Nematicidal compositions and methods
US20040157803A1 (en) * 2002-03-04 2004-08-12 Williams Deryck J. Nematicidal fatty acid and fatty acid ester related compounds
US7368629B2 (en) * 2004-02-04 2008-05-06 Divergence, Inc. Nucleic acids encoding anthelmintic agents and plants made therefrom
MX2007009206A (es) * 2005-01-31 2008-02-19 Athenix Corp Axmi-018, axmi-020 y axmi-021, una familia de genes de delta-endotoxina y metodos para su uso.
BRPI0918840A2 (pt) * 2008-08-25 2015-08-18 Dow Agrosciences Llc Vetor de expressão de planta, proteína modificada, método de produção da referida proteína e método para inibição de um nematoide
WO2010027793A1 (en) * 2008-08-25 2010-03-11 Dow Agrosciences Llc Nematode-resistant plants, and modified bacillus thuringiensis cry genes and proteins
PT2716748T (pt) 2011-05-26 2017-03-21 Sds Biotech Corp Estirpe pertencendo ao género bacillus, agente microbiológico e método de cultivo de plantas
US11692016B2 (en) 2012-03-09 2023-07-04 Vestaron Corporation High gene expression yeast strain
KR20220047395A (ko) 2012-03-09 2022-04-15 베스타론 코포레이션 독성 펩타이드 제조, 식물에서의 펩타이드 발현 및 시스테인 농후 펩타이드의 조합
CN104211790B (zh) * 2013-05-29 2017-10-10 吴小毅 一种高效杀灭同翅目害虫的Bt蛋白Cry21NJ、编码基因及其应用
CN106062175B (zh) 2013-10-17 2019-11-26 出光兴产株式会社 新型微生物及其用途
ES2561908B2 (es) 2014-07-31 2016-12-01 Universidad De Granada Uso de Bacillus methylotrophicus como estimulante del crecimiento vegetal y medio de control biológico, y cepas aisladas de dicha especie
WO2018075269A1 (en) 2016-10-21 2018-04-26 Vestaron Corporation Cleavable peptides and insecticidal and nematicidal proteins comprising same

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4448885A (en) * 1981-04-27 1984-05-15 Board Of The Regents Of The University Of Washington Bacillus thuringiensis crystal protein in Escherichia coli
US4467036A (en) * 1981-11-12 1984-08-21 The Board Of Regents Of The University Of Washington Bacillus thuringiensis crystal protein in Escherichia coli
US4797276A (en) * 1985-03-22 1989-01-10 Mycogen Corporation Control of cotton boll weevil, alfalfa weevil, and corn rootworm via contact with a strain of Bacillus thuringiensis
US4918006A (en) * 1985-07-01 1990-04-17 E. I. Du Pont De Nemours And Company Gene coding for insecticidal crystal protein
US4853331A (en) * 1985-08-16 1989-08-01 Mycogen Corporation Cloning and expression of Bacillus thuringiensis toxin gene toxic to beetles of the order Coleoptera
US5439881A (en) * 1987-08-12 1995-08-08 Mycogen Corporation Gene encoding nematode-active toxin PS63B cloned from Bacillus thuringiensis isolate
US5093120A (en) * 1987-08-12 1992-03-03 Mycogen Corporation Novel isolates of Bacillus thuringiensis having activity against nematodes
US5151363A (en) * 1990-07-27 1992-09-29 Mycogen Corporation Isolates of Bacillus thuringiensis that are active against nematodes
US5322932A (en) * 1987-08-12 1994-06-21 Mycogen Corporation Nematode-active toxin from a Bacillus thuringiensis isolate
US5262399A (en) * 1987-08-12 1993-11-16 Mycogen Corporation Compositions and methods for the control of flukes
US5236843A (en) * 1987-08-12 1993-08-17 Mycogen Corporation Gene encoding a nematode-active toxin cloned from a Bacillus thuringiensis isolate
US5281530A (en) * 1987-08-12 1994-01-25 Mycogen Corporation Genes encoding nematode-active toxins cloned from bacillus thuringiensis isolate PS17
US4948734A (en) * 1987-08-12 1990-08-14 Mycogen Corporation Novel isolates of bacillus thuringiensis having activity against nematodes
US4990332A (en) * 1988-10-25 1991-02-05 Mycogen Corporation Novel lepidopteran-active Bacillus thuringiensis isolate
US5039523A (en) * 1988-10-27 1991-08-13 Mycogen Corporation Novel Bacillus thuringiensis isolate denoted B.t. PS81F, active against lepidopteran pests, and a gene encoding a lepidopteran-active toxin
US5169629A (en) * 1988-11-01 1992-12-08 Mycogen Corporation Process of controlling lepidopteran pests, using bacillus thuringiensis isolate denoted b.t ps81gg
US5164180A (en) * 1989-05-18 1992-11-17 Mycogen Corporation Bacillus thuringiensis isolates active against lepidopteran pests
US5126133A (en) * 1989-06-27 1992-06-30 Mycogen Corporation Bacillus thuringiensis isolate active against lepidopteran pests, and genes encoding novel lepidopteran-active toxins
US5430137A (en) * 1989-10-25 1995-07-04 Mycogen Corporation Probes for the identification of Bacillus thuringiensis endotoxin genes
US5204237A (en) * 1989-10-25 1993-04-20 Mycogen Corporation Universal and specific probes for Bacillus thuringiensis endotoxin genes
US5602032A (en) * 1989-11-17 1997-02-11 Abbott Laboratories Bacillus thuringiensis mutants which produce high yields of crystal delta-endotoxin
CA2042868A1 (en) * 1990-06-11 1991-12-12 Kenneth E. Narva Bacillus thuringiensis microbes active against nematodes, and genes encoding novel nematode-active toxins cloned from bacillus thuringiensis isolates
US5270448A (en) * 1990-07-27 1993-12-14 Mycogen Corporation Isolates of Bacillus thuringiensis that are active against nematodes
US5589382A (en) * 1990-07-27 1996-12-31 Mycogen Corporation Bacillus thuringiensis genes encoding nematode-active toxins
NZ242560A (en) * 1991-05-03 1994-01-26 Mycogen Corp Toxin effective against nematodes and its use in controlling them.
US5596071A (en) * 1991-05-22 1997-01-21 Mycogen Corporation Bacillus thuringiensis toxins active against hymenopteran pests
BR9206032A (pt) * 1991-05-22 1994-12-27 Mycogen Corp Novos isolados de bacillus thuringiensis ativos contra pragas de himenópteros e genes codificando as toxinas ativas para himenópteros
US5554534A (en) * 1991-12-16 1996-09-10 Mycogen Corporation Bacillus thuringiensis toxins active against scarab pests
WO1994016079A2 (en) * 1992-12-31 1994-07-21 Mycogen Corporation Novel bacillus thuringiensis toxins active against corn rootworm larvae
US5527883A (en) * 1994-05-06 1996-06-18 Mycogen Corporation Delta-endotoxin expression in pseudomonas fluorescens

Also Published As

Publication number Publication date
AU7203696A (en) 1997-04-28
EP0853671A1 (en) 1998-07-22
DE69634258T2 (de) 2005-12-22
AU729343C (en) 2003-07-24
JPH11512616A (ja) 1999-11-02
EP0853671B1 (en) 2005-01-26
AU729343B2 (en) 2001-02-01
DE69634258D1 (de) 2005-03-03
ES2235198T3 (es) 2005-07-01
WO1997012980A1 (en) 1997-04-10
US5670365A (en) 1997-09-23
JP4359653B2 (ja) 2009-11-04
ATE287955T1 (de) 2005-02-15
NZ319495A (en) 1999-11-29

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued