CA2230195A1 - A method to increase the density of antigen on antigen presenting cells - Google Patents
A method to increase the density of antigen on antigen presenting cells Download PDFInfo
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- CA2230195A1 CA2230195A1 CA002230195A CA2230195A CA2230195A1 CA 2230195 A1 CA2230195 A1 CA 2230195A1 CA 002230195 A CA002230195 A CA 002230195A CA 2230195 A CA2230195 A CA 2230195A CA 2230195 A1 CA2230195 A1 CA 2230195A1
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| US51737395A | 1995-08-21 | 1995-08-21 | |
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| CA2230195A1 true CA2230195A1 (en) | 1997-02-27 |
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| SE9604581D0 (sv) * | 1996-12-12 | 1996-12-12 | Karolinska Innovations Ab | An agent against cancer and virus infections |
| US6228640B1 (en) | 1997-02-07 | 2001-05-08 | Cem Cezayirli | Programmable antigen presenting cell of CD34 lineage |
| US6251665B1 (en) | 1997-02-07 | 2001-06-26 | Cem Cezayirli | Directed maturation of stem cells and production of programmable antigen presenting dentritic cells therefrom |
| US6977073B1 (en) | 1997-02-07 | 2005-12-20 | Cem Cezayirli | Method for stimulating an immune response |
| HUP0002724A3 (en) * | 1997-08-15 | 2001-04-28 | Millennium Pharm Inc | Clasto-lactacystin betha-lactone, analogs and intermediates thereof, process for their preparation and pharmaceutical compositions containing them |
| US6133308A (en) * | 1997-08-15 | 2000-10-17 | Millennium Pharmaceuticals, Inc. | Synthesis of clasto-lactacystin beta-lactone and analogs thereof |
| US7390619B1 (en) | 1998-02-11 | 2008-06-24 | Maxygen, Inc. | Optimization of immunomodulatory properties of genetic vaccines |
| EP1518927A3 (en) * | 1998-02-11 | 2005-06-22 | Maxygen, Inc. | Optimization of immunomodulatory properties of genetic vaccines |
| AU2674299A (en) * | 1998-02-11 | 1999-08-30 | Maxygen, Inc. | Targeting of genetic vaccine vectors |
| JP2002506622A (ja) * | 1998-03-20 | 2002-03-05 | ジェンザイム・コーポレーション | 抗原提示細胞上での亢進した抗原提示の方法、およびそれにより産生する組成物 |
| US6436392B1 (en) * | 1998-05-20 | 2002-08-20 | University Of Iowa Research Foundation | Adeno-associated virus vectors |
| GB9817693D0 (en) * | 1998-08-14 | 1998-10-07 | Bridgehead Technologies Limite | Virally-mediated targeting of drugs and genetic material |
| EP1153612A4 (en) * | 1999-02-10 | 2003-06-18 | Takashi Tsuruo | Anticancer agent potentiators |
| JP4969002B2 (ja) * | 1999-06-08 | 2012-07-04 | ユニバーシテイ・オブ・アイオワ・リサーチ・フアウンデーシヨン | ｒＡＡＶ形質導入を増加するための化合物および方法 |
| US7122335B1 (en) * | 1999-06-08 | 2006-10-17 | University Of Iowa Research Foundation | Compounds and methods to enhance rAAV transduction |
| AT408721B (de) * | 1999-10-01 | 2002-02-25 | Cistem Biotechnologies Gmbh | Pharmazeutische zusammensetzung enthaltend ein antigen |
| US7241447B1 (en) | 1999-10-07 | 2007-07-10 | University Of Iowa Research Foundation | Adeno-associated virus vectors and uses thereof |
| US6649593B1 (en) * | 1999-10-13 | 2003-11-18 | Tularik Inc. | Modulators of SREBP processing |
| AU2004227358B8 (en) * | 2003-03-31 | 2009-12-10 | Targeted Genetics Corporation | Compounds and methods to enhance rAAV transduction |
| US20060167106A1 (en) * | 2004-11-19 | 2006-07-27 | Mei Zhang | Compounds acting at the centrosome |
| WO2007120863A2 (en) | 2006-04-14 | 2007-10-25 | Epicentre Technologies | Kits and methods for generating 5' capped rna |
| CA2651180A1 (en) * | 2006-04-28 | 2007-11-08 | University Of Iowa Research Foundation | Methods and compounds to alter virus infection |
| US20140010821A1 (en) | 2006-08-18 | 2014-01-09 | Aili Lao | Use of CD83 in combination therapies |
| EP2557089A2 (en) | 2011-07-15 | 2013-02-13 | Fundació Institut d'Investigació Biomèdica de Bellvitge (IDIBELL) | Compositions and methods for immunomodulation |
| US11702672B2 (en) | 2016-02-08 | 2023-07-18 | University Of Iowa Research Foundation | Methods to produce chimeric adeno-associated virus/bocavirus parvovirus |
| EP3426787A1 (en) | 2016-03-07 | 2019-01-16 | University of Iowa Research Foundation | Aav-mediated expression using a synthetic promoter and enhancer |
| US12173305B2 (en) | 2016-05-26 | 2024-12-24 | University Of Iowa Research Foundation | cis and trans requirements for terminal resolution of human bocavirus 1 |
| US11142775B2 (en) | 2017-01-13 | 2021-10-12 | University Of Iowa Research Foundation | Bocaparvovirus small noncoding RNA and uses thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU660422B2 (en) * | 1990-09-06 | 1995-06-29 | Immulogic Pharmaceutical Corporation | Pathogen-specific CTL therapy |
| DE69430315T2 (de) * | 1993-08-06 | 2002-11-21 | Epimmune, Inc. | Methoden zur (ex vivo) therapie mittels peptid - bestueckten antigen - praesentierenden zellen zur aktivierung von ctl |
| WO1995006744A2 (en) * | 1993-09-03 | 1995-03-09 | Chiron Viagene, Inc. | Methods of suppressing immune response by gene therapy |
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1996
- 1996-08-20 US US08/700,035 patent/US5831068A/en not_active Expired - Fee Related
- 1996-08-20 AU AU70088/96A patent/AU7008896A/en not_active Abandoned
- 1996-08-20 WO PCT/US1996/013457 patent/WO1997007128A1/en not_active Ceased
- 1996-08-20 EP EP96931395A patent/EP0863913A4/en not_active Withdrawn
- 1996-08-20 CA CA002230195A patent/CA2230195A1/en not_active Abandoned
- 1996-08-20 JP JP9509560A patent/JPH11511968A/ja not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| EP0863913A4 (en) | 2001-04-11 |
| US5831068A (en) | 1998-11-03 |
| JPH11511968A (ja) | 1999-10-19 |
| EP0863913A1 (en) | 1998-09-16 |
| AU7008896A (en) | 1997-03-12 |
| WO1997007128A1 (en) | 1997-02-27 |
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