CA2207278A1 - Method of producing and purifying enzymes - Google Patents
Method of producing and purifying enzymesInfo
- Publication number
- CA2207278A1 CA2207278A1 CA 2207278 CA2207278A CA2207278A1 CA 2207278 A1 CA2207278 A1 CA 2207278A1 CA 2207278 CA2207278 CA 2207278 CA 2207278 A CA2207278 A CA 2207278A CA 2207278 A1 CA2207278 A1 CA 2207278A1
- Authority
- CA
- Canada
- Prior art keywords
- enzyme
- host organism
- producing
- spores
- dna sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108090000790 Enzymes Proteins 0.000 title abstract 9
- 102000004190 Enzymes Human genes 0.000 title abstract 9
- 238000000034 method Methods 0.000 title abstract 2
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
- 230000002068 genetic effect Effects 0.000 abstract 2
- 108010093096 Immobilized Enzymes Proteins 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 239000012535 impurity Substances 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- 230000028070 sporulation Effects 0.000 abstract 1
- 238000003786 synthesis reaction Methods 0.000 abstract 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
A method of producing and purifying an enzyme which comprises selecting a spore forming host organism, preparing a genetic construct consisting of a DNA sequence encoding the desired enzyme and a DNA sequence directing synthesis of the desired enzyme during sporulation, inserting the genetic construct into the host organism, culturing the transformed host organism under sporulating conditions to obtain host organism spores with the enzyme integrally associated to the spores, and then treating the host organism and enzyme combination to remove any impurities, if necessary. The free enzyme can be obtained by cleaving the connection between the host organism and the enzyme. The combination of the enzyme and host organism is both a stabilized and an immobilized enzyme preparation.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/378,698 US5766914A (en) | 1995-01-26 | 1995-01-26 | Method of producing and purifying enzymes |
| US08/378,698 | 1995-01-26 | ||
| PCT/US1996/000728 WO1996023063A1 (en) | 1995-01-26 | 1996-01-22 | Method of producing and purifying enzymes |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2207278A1 true CA2207278A1 (en) | 1996-08-01 |
| CA2207278C CA2207278C (en) | 2000-03-28 |
Family
ID=29552594
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA 2207278 Expired - Fee Related CA2207278C (en) | 1995-01-26 | 1996-01-22 | Method of producing and purifying enzymes |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2207278C (en) |
-
1996
- 1996-01-22 CA CA 2207278 patent/CA2207278C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CA2207278C (en) | 2000-03-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |