CA2204641A1 - Detection d'acides nucleiques dans des cellules par amplification par deplacement de brins thermophiles - Google Patents

Detection d'acides nucleiques dans des cellules par amplification par deplacement de brins thermophiles

Info

Publication number
CA2204641A1
CA2204641A1 CA 2204641 CA2204641A CA2204641A1 CA 2204641 A1 CA2204641 A1 CA 2204641A1 CA 2204641 CA2204641 CA 2204641 CA 2204641 A CA2204641 A CA 2204641A CA 2204641 A1 CA2204641 A1 CA 2204641A1
Authority
CA
Canada
Prior art keywords
target sequence
amplification
amplification primer
seq
restriction endonuclease
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2204641
Other languages
English (en)
Inventor
Kenton L. Lohman
Mark Van Cleve
Robert Alan Reid
Natalie V. Ostrerova
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/531,749 external-priority patent/US5733752A/en
Priority claimed from US08/531,747 external-priority patent/US5631147A/en
Application filed by Individual filed Critical Individual
Priority to CA002305858A priority Critical patent/CA2305858A1/fr
Publication of CA2204641A1 publication Critical patent/CA2204641A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • AIDS & HIV (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Cette invention se rapporte à l'amplification par déplacement de brins thermophiles (tSDA) qui permet l'amplification de séquences cibles d'acides nucléiques in situ dans des cellules en suspension, sur des lamelles ou dans des tissus. On préserve l'excellente structure morphologique d'échantillons, et il est possible d'amplifier sélectivement soit des cibles d'ADN, soit des cibles d'ARN, soit ces deux types de cibles. L'amplification in situ par tSDA est compatible avec les techniques immunochimiques, de telle sorte qu'il est possible d'effectuer à la fois l'amplification des séquences cibles et la coloration immunologique sur le même échantillon.
CA 2204641 1995-09-21 1996-09-12 Detection d'acides nucleiques dans des cellules par amplification par deplacement de brins thermophiles Abandoned CA2204641A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA002305858A CA2305858A1 (fr) 1995-09-21 1996-09-12 Detection d'acides nucleiques dans des cellules par amplification par de placement de brins thermophiles

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US08/531,747 1995-09-21
US08/531,749 US5733752A (en) 1993-12-10 1995-09-21 Detection of nucleic acids in cells by thermophilic strand displacement amplification
US08/531,749 1995-09-21
US08/531,747 US5631147A (en) 1995-09-21 1995-09-21 Detection of nucleic acids in cells by thermophilic strand displacement amplification

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA002305858A Division CA2305858A1 (fr) 1995-09-21 1996-09-12 Detection d'acides nucleiques dans des cellules par amplification par de placement de brins thermophiles

Publications (1)

Publication Number Publication Date
CA2204641A1 true CA2204641A1 (fr) 1997-03-27

Family

ID=27063626

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2204641 Abandoned CA2204641A1 (fr) 1995-09-21 1996-09-12 Detection d'acides nucleiques dans des cellules par amplification par deplacement de brins thermophiles

Country Status (6)

Country Link
EP (1) EP0796347A2 (fr)
JP (2) JP3092163B2 (fr)
AU (1) AU702896B2 (fr)
BR (1) BR9606653A (fr)
CA (1) CA2204641A1 (fr)
WO (1) WO1997011196A2 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5916779A (en) * 1995-09-21 1999-06-29 Becton, Dickinson And Company Strand displacement amplification of RNA targets
US6187567B1 (en) * 1999-09-01 2001-02-13 Akzo Nobel N.V. Methods and reagents for in situ amplification
JP2002159295A (ja) * 2000-11-27 2002-06-04 Keio Gijuku Ｈｉｖ−１ｒｎａ−ｄｎａハイブリッドの定量法および診断キット
ATE319731T1 (de) * 2001-04-13 2006-03-15 Becton Dickinson Co Amplifikation und nachweis von mycoplasma pneumoniae
MXPA04000432A (es) 2001-07-15 2004-10-27 Keck Graduate Inst Amplificacion de fragmentos de acido nucleico empleando agentes de mella.
CA2492220C (fr) 2001-07-15 2014-03-18 Keck Graduate Institute Amplification d'acides nucleiques a l'aide d'agents de synchronisation
EP2071038B1 (fr) * 2003-04-25 2014-11-26 Becton Dickinson and Company Détection de virus d'herpes simplex de types 1 et 2 par amplification d'acides nucléiques
CN1918305B (zh) * 2004-02-09 2010-12-01 扶桑药品工业株式会社 核酸检测方法及其应用
FR2878254B1 (fr) * 2004-11-22 2010-08-20 Bio Rad Pasteur Composition pour l'amplification d'acides nucleiques
DK2473596T3 (en) 2009-09-03 2018-01-22 Becton Dickinson Co METHODS AND COMPOSITIONS FOR DIRECT CHEMICAL LYSIS
CN112391450A (zh) * 2020-11-19 2021-02-23 四川大学 一种基于修饰dNTP的高特异和高灵敏核酸检测方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1284931C (fr) * 1986-03-13 1991-06-18 Henry A. Erlich Procede de detection de variations specifiques de nucleotides et de polymorphismes genetiques dans les acides nucleiques
US5043272A (en) * 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
GB9116042D0 (en) * 1991-07-24 1991-09-11 Royal Free Hosp School Med Diagnostic method
US5270184A (en) * 1991-11-19 1993-12-14 Becton, Dickinson And Company Nucleic acid target generation
KR100325554B1 (ko) * 1993-03-26 2002-11-02 젠-프로브 인코포레이티드 사람의면역결핍 바이러스타입1의검출
US5523204A (en) * 1993-12-10 1996-06-04 Becton Dickinson And Company Detection of nucleic acids in cells by strand displacement amplification
AU687535B2 (en) * 1994-03-16 1998-02-26 Gen-Probe Incorporated Isothermal strand displacement nucleic acid amplification

Also Published As

Publication number Publication date
JP2000300281A (ja) 2000-10-31
EP0796347A2 (fr) 1997-09-24
MX9703730A (es) 1997-09-30
BR9606653A (pt) 1997-11-04
WO1997011196A2 (fr) 1997-03-27
WO1997011196A3 (fr) 1997-05-09
JP3092163B2 (ja) 2000-09-25
AU7019296A (en) 1997-04-09
AU702896B2 (en) 1999-03-11
JPH10500313A (ja) 1998-01-13

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Legal Events

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