CA2199131C - Hybrides vectoriels retroviraux et leur utilisation pour le transfert de genes - Google Patents

Hybrides vectoriels retroviraux et leur utilisation pour le transfert de genes Download PDF

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Publication number
CA2199131C
CA2199131C CA002199131A CA2199131A CA2199131C CA 2199131 C CA2199131 C CA 2199131C CA 002199131 A CA002199131 A CA 002199131A CA 2199131 A CA2199131 A CA 2199131A CA 2199131 C CA2199131 C CA 2199131C
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CA
Canada
Prior art keywords
virus
region
mesv
binding site
ltr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA002199131A
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English (en)
Other versions
CA2199131A1 (fr
Inventor
Christopher Baum
Carol Stocking-Harbers
Wolfram Ostertag
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Vision 7 GmbH
Original Assignee
Vision 7 GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19503952A external-priority patent/DE19503952A1/de
Application filed by Vision 7 GmbH filed Critical Vision 7 GmbH
Priority claimed from PCT/EP1995/003175 external-priority patent/WO1996007747A1/fr
Publication of CA2199131A1 publication Critical patent/CA2199131A1/fr
Application granted granted Critical
Publication of CA2199131C publication Critical patent/CA2199131C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/13043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Hybrides vectoriels rétroviraux à défauts de réplication, caractérisés en ce qu'ils contiennent a) dans la région leader, comme région U5 et/ou comme site de liaison amorce tRNA, la région U5 et/ou le site de liaison amorce tRNA de MESV et, b) comme régions U3 et R en 3'-LTR, les régions U3 et R d'un virus de leucémie murine Friend (F-MuLV). Les hybrides selon l'invention sont particulièrement appropriés pour un transfert efficace de gènes dans des cellules souches hématopoïétiques.
CA002199131A 1994-09-08 1995-08-10 Hybrides vectoriels retroviraux et leur utilisation pour le transfert de genes Expired - Fee Related CA2199131C (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DEP4431973.8 1994-09-08
DE4431973 1994-09-08
DE19503952A DE19503952A1 (de) 1994-09-08 1995-02-07 Retrovirale Vektorhybride und deren Verwendung zum Gentransfer
DE19503952.1 1995-02-07
PCT/EP1995/003175 WO1996007747A1 (fr) 1994-09-08 1995-08-10 Hybrides vectoriels retroviraux et leur utilisation pour le transfert de genes

Publications (2)

Publication Number Publication Date
CA2199131A1 CA2199131A1 (fr) 1996-03-14
CA2199131C true CA2199131C (fr) 2007-04-10

Family

ID=38007181

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002199131A Expired - Fee Related CA2199131C (fr) 1994-09-08 1995-08-10 Hybrides vectoriels retroviraux et leur utilisation pour le transfert de genes

Country Status (1)

Country Link
CA (1) CA2199131C (fr)

Also Published As

Publication number Publication date
CA2199131A1 (fr) 1996-03-14

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