CA2183657A1 - Culture et isolation de cellules foetales a partir de sang peripherique maternel - Google Patents

Culture et isolation de cellules foetales a partir de sang peripherique maternel

Info

Publication number
CA2183657A1
CA2183657A1 CA002183657A CA2183657A CA2183657A1 CA 2183657 A1 CA2183657 A1 CA 2183657A1 CA 002183657 A CA002183657 A CA 002183657A CA 2183657 A CA2183657 A CA 2183657A CA 2183657 A1 CA2183657 A1 CA 2183657A1
Authority
CA
Canada
Prior art keywords
cells
fetal
sample
erythroid
peripheral blood
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002183657A
Other languages
English (en)
Inventor
Karen Pavelka
Anna Mahr
Katherine W. Klinger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genzyme Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2183657A1 publication Critical patent/CA2183657A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0641—Erythrocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/14—Erythropoietin [EPO]

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne des procédés de culture et d'isolation de cellules foetales, par exemple de cellules foetales de la lignée érythrocytaire, à partir d'un échantillon de sang périphérique prélevé sur une femme enceinte. Ces cellules provenant de l'échantillon de sang périphérique sont mises en culture dans un milieu contenant un facteur de croissance cellulaire, par exemple un facteur de croissance érythrocytaire tel que l'érythropoïétine, en vue de la culture de cellules foetales. Avant la culture, les cellules anucléées peuvent être retirées de l'échantillon de sang périphérique et/ou les cellules foetales peuvent être enrichies. Des propagations de cellules au stade de la métaphase peuvent être préparées à partir de cellules foetales de culture. Un marqueur de cellule foetale ou une séquence d'acide nucléique présentant un intérêt peuvent être détectés dans les cellules foetales de culture. Ces dernières peuvent être utilisées pour déterminer le sexe du foetus et pour détecter des mutations pathogènes et des anomalies chromosomiques dans un foetus.
CA002183657A 1994-03-29 1995-03-29 Culture et isolation de cellules foetales a partir de sang peripherique maternel Abandoned CA2183657A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US21950594A 1994-03-29 1994-03-29
US08/219,505 1994-03-29

Publications (1)

Publication Number Publication Date
CA2183657A1 true CA2183657A1 (fr) 1995-10-05

Family

ID=22819541

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002183657A Abandoned CA2183657A1 (fr) 1994-03-29 1995-03-29 Culture et isolation de cellules foetales a partir de sang peripherique maternel

Country Status (5)

Country Link
EP (1) EP0804614A1 (fr)
JP (1) JPH09510875A (fr)
AU (1) AU2274595A (fr)
CA (1) CA2183657A1 (fr)
WO (1) WO1995026417A1 (fr)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1294964B1 (it) * 1996-07-12 1999-04-23 Domenico Valerio Isolamento e cultura di cellule fetali dal sangue periferico materno
CA2445204C (fr) 2002-10-16 2014-08-12 Streck Laboratories, Inc. Methode et dispositif de prelevement et de conservation de cellules aux fins d'analyse
CA2998199A1 (fr) * 2004-06-01 2005-12-15 Kwalata Trading Limited Procedes destines a etre utilises avec des cellules souches impliquant la culture sur une surface avec des anticorps
EP2094838A1 (fr) * 2006-10-16 2009-09-02 Cellula, Inc. Procédés et compositions pour le développement différentiel de cellules foetales dans le sang maternel et leur utilisation
MX2010003116A (es) * 2007-09-21 2010-08-03 Biocept Inc Identificacion y aislamiento de celulas fetales y acido nucleico.
US11634747B2 (en) 2009-01-21 2023-04-25 Streck Llc Preservation of fetal nucleic acids in maternal plasma
EP3290530B1 (fr) 2009-02-18 2020-09-02 Streck Inc. Préservation d'acides nucléiques acellulaires
JP5527802B2 (ja) * 2009-11-24 2014-06-25 株式会社シームス 疾病予測システム
WO2012151391A2 (fr) 2011-05-04 2012-11-08 Streck, Inc. Compositions virales inactivées et leurs procédés de préparation
WO2012174225A2 (fr) * 2011-06-14 2012-12-20 The University Of North Carolina At Chapel Hill Isolement, expansion et utilisation de cellules souches pluripotentes autologues
EP4008270A1 (fr) 2012-06-22 2022-06-08 Preprogen LLC Procédé d'obtention de cellules foetales et composants cellulaires foetaux
WO2015013244A1 (fr) 2013-07-24 2015-01-29 Streck, Inc. Compositions et méthodes de stabilisation de cellules tumorales circulantes
JP2016136921A (ja) * 2015-01-29 2016-08-04 大日本印刷株式会社 細胞培養容器
WO2016125924A1 (fr) * 2015-02-03 2016-08-11 한국기초과학지원연구원 Dispositif et procédé de refroidissement d'échantillon permettant une détection d'image liée par un microscope optique et un microscope électronique
US11168351B2 (en) 2015-03-05 2021-11-09 Streck, Inc. Stabilization of nucleic acids in urine
US20170145475A1 (en) 2015-11-20 2017-05-25 Streck, Inc. Single spin process for blood plasma separation and plasma composition including preservative
WO2018022991A1 (fr) 2016-07-29 2018-02-01 Streck, Inc. Composition de suspension pour contrôle d'analyse hématologique

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2081203A1 (fr) * 1990-04-23 1991-10-24 Ronald J. Berenson Methode permettant d'enrichir les cellules d'un foetus a partir du sang maternel
CA2122138A1 (fr) * 1991-10-23 1993-04-29 Sheryl Williams Methode d'enrichissement de cellules foetales du sang maternel
US5275933A (en) * 1992-09-25 1994-01-04 The Board Of Trustees Of The Leland Stanford Junior University Triple gradient process for recovering nucleated fetal cells from maternal blood

Also Published As

Publication number Publication date
JPH09510875A (ja) 1997-11-04
WO1995026417A1 (fr) 1995-10-05
AU2274595A (en) 1995-10-17
EP0804614A1 (fr) 1997-11-05

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Legal Events

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